Classifcation of atrial fbrillation and normal sinus rhythm based on convolutional neural network

Mei-Ling Huang,Yan-Sheng Wu 대한의용생체공학회 2020 Biomedical Engineering Letters (BMEL) Vol.10 No.2

Electrocardiogram (ECG) technology plays a vital role in detecting arrhythmia. Numerous achievements have been marked in ECG-related research. Most methods frst pre-process ECG signals, then extract features, and fnally classify them. Most of the ECG signals used in the related studies were analyzed in specifc time intervals or using a fxed number of samples. However, it is not always possible to see signifcant changes in a short term, and the symptoms of some patients are relatively short-lived. Misjudgments are possible because the ECG signal was not accurately extracted. This study proposes a computeraided diagnosis (CAD) system for classifcation of Atrial Fibrillation and Normal Sinus Rhythm based on ECG signals through convolutional neural network. The proposed system considers a single heartbeat, rather than a specifc number of seconds. This study eschews the one-dimensional digital ECG signal used in previous studies and uses convolutional neural networks to analyze two-dimensional ECG image. This study explores whether two-dimensional image ECG requires signal fltering. The fnal classifcation results in fltered ECG signals is accuracy of 99.23%, sensitivity of 99.71%, and specifcity of 98.66%. The best result in non-fltered ECG signals achieves accuracy of 99.18%, sensitivity of 99.31%, and specifcity of 99.03%. With no cumbersome artifcial settings, the results of this study are comparable to the related studies. The proposed CAD system has high generalizability; it can help doctors to diagnose diseases efectively and reduce misdiagnosis.

Heart Rate Variability Biofeedback Increased Autonomic Activation and Improved Symptoms of Depression and Insomnia among Patients with Major Depression Disorder

I-Mei Lin,Sheng-Yu Fan,Cheng-Fang Yen,Yi-Chun Yeh,Tze‐Chun Tang,Mei-Feng Huang,Tai-Ling Liu,Peng-Wei Wang,Huang-Chi Lin,Hsin-Yi Tsai,Yu-Che Tsai 대한정신약물학회 2019 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.17 No.2

Objective: Autonomic imbalance is considered a psychopathological mechanism underlying major depressive disorder (MDD). Heart rate variability (HRV) is an index for autonomic activation. Poor sleep quality is common among patients with MDD. HRV biofeedback (BF) has been used for regulating autonomic balance among patients with physical illness and mental disorders. The purpose of present study was to examine the effects of HRV-BF on depressive symptoms, sleep quality, pre-sleep arousal, and HRV indices, in patients with MDD and insomnia. Methods: In this case-controlled study, patients with MDD and Pittsburgh Sleep Quality Index (PSQI) score higher than 6 were recruited. The HRV-BF group received weekly 60-minute protocol for 6 weeks, and the control group who have matched the age and sex received medical care only. All participants were assessed on Beck Depression Inventory-II, Back Anxiety Inventory, PSQI, and Pre-Sleep Arousal Scale. Breathing rates and electrocardiography were also performed under resting state at pre-testing, and post-testing conditions and for the HRV-BF group, also at 1-month follow-up. Results: In the HRV-BF group, symptoms of depression and anxiety, sleep quality, and pre-sleep arousal were significantly improved, and increased HRV indices, compared with the control group. Moreover, in the HRV-BF group, significantly improved symptoms of depression and anxiety, decreased breathing rates, and increased HRV indices were detected at post-testing and at 1-month follow-up, compared with pre-testing values. Conclusion: This study confirmed that HRV-BF is a useful psychosocial intervention for improving autonomic balance, baroreflex, and symptoms of depression and insomnia in MDD patients.

Cloning, Expression, and Characterization of Endoglucanase Gene egIV from Trichoderma viride AS 3.3711

( Huang Xiao Mei ),( Jin Xia Fan ),( Qian Yang ),( Xiu Ling Chen ),( Zhi Hua Liu ),( Yun Wang ),( Da Qing Wang ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.3

Endoglucanase gene egIV was cloned from Trichoderma viride AS 3.3711, an important cellulose-producing fungus, by using an RT-PCR protocol. The egIV cDNA is 1,297 bp in length and contains a 1,035 bp open reading frame encoding a 344 amino acid protein with an estimated molecular mass of 35.5 kDa and isoelectronic point (pI) of 5.29. The expression of gene egIV in T. viride AS 3.3711 could be induced by sucrose, corn straw, carboxymethylcellulose (CMC), or microcrystalline cellulose, but especially by CMC. The transcripts of egIV were regulated under these substrates, but the expression level of the egIV gene could be inhibited by glucose and fructose. Three recombinant vectors, pYES2-xegIV, pYES2Mα-egIV, and pYES2Mα-xegIV, were constructed to express the egIV gene in Saccharomyces cerevisiae H158. The CMCase activity of yeast transformants IpYES2Mα-xegIV was higher than that of transformant IpYES2-xegIV or IpYES2Mα-egIV, with the highest activity of 0.13 U/ml at induction for 48 h, illustrating that the modified egIV gene could enhance CMCase activity and that MFα signal peptide from S. cerevisiae could regulate exogenous gene expression more effectively in S. cerevisiae. The recombinant EGIV enzyme was stable at pH 3.5 to 7.5 and temperature of 35oC to 65oC. The optimal reaction condition for EGIV enzyme activity was at the temperature of 55oC, pH of 5.0, 0.75 mM Ba2+, and using CMC as substrate. Under these conditions, the highest activity of EGIV enzyme in transformant IpYES2Mα-xegIV was 0.18 U/ml. These properties would provide technical parameters for utilizing cellulose in industrial bioethanol production.

Optomizing Transfection Efficiency of Cervical Cancer Cells Transfected by Cationic Liposomes Lipofectamine^TM2000

Huang, Fei,Zhao, Feng,Liang, Li-Ping,Zhou, Mei,Qu, Zhi-Ling,Cao, Yan-Zhen,Lin, Chen Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Background: Currently, cationic liposome has become the commonly used vehicles for gene transfection. Furthermore, one of the most significant steps in microRNAs expression studies is transferring microRNAs into cell cultures successfully. In this study we aim to approach the feasibility of transfection of cervical cancer cell lines mediated by liposome and to obtain the optimized transfection condition for cervical cancer cell lines. Materials and Methods: $Lipofectamine^{TM}2000$ as the carrier, miR-101 mimic was transfected into Hela cells and Siha cells. Using green fluorescent protein as reporter gene, to set different groups according to cell seeding density, the amount of miRNA, miRNA and the proportion of Liposomes, Whether to add serum into medium to study their impact on the liposomal transfection efficiency. Finally, MTT assay was used to analyze the relative minimal cell toxicity of liposome reagents. Results: The seeding density of Hela cell line and Siha are $1.5{\times}10^4$ (per well of 24 well plates), miRNA amount is 1ul of both, the ratio of miRNA and liposome is 1:0.5 of Hela cell line; 1:0.7 of Siha cell line respectively, after 24 hours we can get the highest transfection efficiency. Compared with serum medium, only Siha cells cultured with serum-free medium obtained higher transfection efficiency before transfection (P<0.01). MTT assay showed that according to the above conditions which has the lowest cytotoxicity. Conclusions: The method of Liposome to transfected is a suitable way and it can be an efficient reagent for miRNA delivery for Hela cells and Siha cells in vitro. It may serve as a reference for the further research or application.

The complete mitochondrial genome sequence of the little grebe (Tachybaptus ruficollis)

Mei-dong Jing,Ling Huang,Yi-cheng Wang,Yi Zou,Xiao-min Sun,Jie Gong 한국유전학회 2017 Genes & Genomics Vol.39 No.1

Podicipediformes comprises one family (Podicipedidae) including 6 genera, 22 species, and the phylogenetic placement of this order was still in debate. In this study, we sequenced the complete mitochondrial genome (mitogenome) of little grebe (Tachybaptus ruficollis) in Podicipediformes, and explored the phylogenetic position of this order with mitogenome sequences of 21 species from ten families in seven orders. The genome was 16,688 bp in length, and contained 37 genes typical to avian mitogenomes and one control region. The gene organization and characters were similar with other two mitogenomes available in Podicipediformes to date. Phylogenetic tree was constructed with Bayesian method based on mitogenome sequences excluding the control regions. The results supported the closest relationship between Podicipediformes and Phoenicopteriformes, and the topology of our tree was generally similar with the conclusions of previous molecular systematic investigations. Our results furtherly proved the validity of mitogenome data in taxonomic and phylogenetic studies.

A Project in Increasing the Self-Monitoring Rate of Outpatient Adult Asthma Patients at Home

Chia-Ling Hsieh,Chin-Chain Huang,Mei-Chuan Wu,Su-Chin Ho 한국간호과학회 2009 한국간호과학회 학술대회 Vol.2009 No.10

Vibration Frequency Matching in Fiber-based Interferometer for Femtogram Measurement

Jing-Yi Huang,Mei-Ling Chen,J. Andrew Yeh 제어로봇시스템학회 2006 제어로봇시스템학회 국제학술대회 논문집 Vol.2006 No.10

Two mitogenomes in Gruiformes (Amaurornis akool/A. phoenicurus) and the phylogenetic placement of Rallidae

Jie Gong,Ling Huang,Ruoping Zhao,Qingrong Huang,Xiaomin Sun,Mei-dong Jing 한국유전학회 2017 Genes & Genomics Vol.39 No.9

Rallidae, with 34 genera including 142 species, is the largest family in the Gruiformes, the phylogenetic placement of this family was still in debate. The complete mitochondrial genomes (mitogenomes), with many advantageous characters, have become popular markers in phylogenetic analyses. We sequenced the mitogenomes of brown crake (Amaurornis akool) and white-breasted waterhen (Amaurornis phoenicurus), analyzed the genomic characters of mitogenomes in Rallidae, and explored the phylogenetic relationships between Rallidae and other four families in Gruiformes based on mitogenome sequences of 32 species with Bayesian method. The mitogenome of A. akool/A. phoenicurus was 16,950/17,213 bp in length, and contained 37 genes typical to avian mitogenomes and one control region, respectively. The genomic characters of mitogenomes in Rallidae were similar. The phylogenetic results indicated that, among five families, Rallidae had closest relationship with Heliornithidae, which formed a sister taxa to Gruidae, while Rhynochetidae located in the basal lineage. Within Rallidae, Rallina was ancestral clade. Gallirallus & Rallus and Aramides were closely related, Gallicrex & Amaurornis and Fulica & Gallinula had close relationships, and these two taxa formed a sister clade to Porphyrio & Coturnicops. Our phylogenetic analyses provided solid evidence for the phylogenetic placement of Rallidae and the evolutionary relationships among different genus within this family. In addition, the mitogenome data presented here provide useful information for further molecular systematic investigations on Gruiformes as well as conservation biology research of these species.

Micellization behavior of binary mixtures of amino sulfonate amphoteric surfactant with different octylphenol polyoxyethylene ethers in aqueous salt solution: Both cationic and hydrophilic effects

Zhao Hua Ren,Jing Huang,Yue Luo,Yan Cheng Zheng,Ping Mei,Lu Lai,Yan Ling Chang 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.36 No.-

The effect of different valent inorganic cations and the hydrophilic group of surfactant on themicellization behavior of binary surfactant mixtures constituted by an amphoteric surfactant, sodium3-(N-dodecyl ethylenediamino)-2-hydropropyl sulfonate (C12AS), and three nonionic surfactantsoctylphenol polyoxyethylene ether (OP-n) with different numbers of oxyethylene glycol ethers (n),namely, OP-10, OP-7, and OP-4 was investigated in aqueous solution. These inorganic cations includeNa+, K+, Mg2+, Ca2+, Ba2+, Al3+ and Fe3+. Both the tensiometry and the UV–vis spectrophotometry usingpyrene as a probe were adopted to determine the critical micelle concentrations (cmc) of individual ormixed surfactants. Interaction parameters between two surfactants and other parameters were obtainedbased on the regular solution theory, the pseudophase separation model, Rubingh’s model, etc. Thermodynamic parameters including Gibbs energy of micellization and thermodynamic stability werealso calculated by both the equation proposed by Molyneux et al. and Maeda’s treatment, respectively. The effect of different valent cations on both the mixed cmc of surfactant mixtures and the molar fractionof C12AS in mixed micelle (X1) can be explained theoretically by the salting-out effect, electrostaticinteraction and steric effect. For three binary surfactant mixtures, the chain length of hydrophilic groupof surfactant results in different micellization behaviors. On adding salts, the interaction parametersshow that there exists a synergistic effect between two surfactants, and the effect increases withincreasing the ratio of valence (Z) and atom radius (R) of cation, especially, for the C12AS/OP-4 mixture. With increasing the Z/R value of cation, a deviation of X1 from the ideal value shows a divergence forthree binary surfactant mixtures because of different hydrophilicities of surfactant. Thermodynamicparameters indicate that the addition of cations with a large Z/R value can be contributive moreeffectively to the formation of stable mixed micelle than the case in the presence of cations with a smallZ/R value.

Effects of inclusion level and amino acid supplementation on energy values of soybean oil determined with difference or regression methods in growing pigs

Qiuyun Wang,Chengfei Huang,Mei Liu,Ling Liu,Shuai Zhang 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11

Objective: This study was conducted to evaluate the effects of inclusion level and amino acid (AA) supplementation on energy values of soybean oil (SO) as determined by difference method or regression method when fed to growing pigs. Methods: Thirty-six barrows (initial body weight: 28.0±1.3 kg) were randomly assigned to one of 6 dietary treatments, which included 2 control diets formulated using a basal diet with or without AA supplementation, and 4 experimental diets with 5% or 10% SO addition in the 2 control diets, respectively. All pigs were individually housed in metabolism crates for 19 d, and during the last 5 d, total urine and feces production were collected. The nutrient digestibility in diets and the digestible energy (DE) and metabolizable energy (ME) values of SO were determined using the difference method and the regression method, respectively. Results: Our results showed that there were no interaction effects (p>0.05) between AA supplementation and SO inclusion levels on energy values of SO and dietary nutrient digestibility. The DE and ME values of SO determined by the difference method were not affected (p>0.05) by AA supplementation, however, the ME value of SO increased (p<0.05) as the inclusion level of SO increased. Moreover, the energy values of SO determined using the regression method were close to those determined using difference method with 10% SO inclusion, but were greater than those obtained using difference method with 5% SO inclusion. Conclusion: We concluded that the DE and ME values of SO increased with the inclusion level but were not affected by AA supplementation in the range of 0% to 10%. The difference method can substitute for the regression method to determine the DE and ME values of SO when the inclusion level is 10%, but not at 5% inclusion level.