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Islam, Md. Taohidul,Mohiuddin, Mohammad,Hossain, Muhammad Tofazzal,Rahman, Md. Bahanur,Rahman, Md. Mostafizur,Rahman, Md. Siddiqur,Song, Hee-Jong,Islam, Md. Alimul The Korean Society of Veterinary Service 2012 韓國家畜衛生學會誌 Vol.35 No.1
The objective of the present study was to isolate and identify infectious bursal disease viruses (IBDVs) from broiler and layer chickens of outbreaks of infectious bursal disease (IBD) in three districts of Bangladesh. A total of 70 bursal samples were collected from dead broiler (n=40) and layer (n=30) chickens showing specific lesions of IBD from seven commercial poultry farms of three different districts (Mymensingh, Chittagong and Tangail) of Bangladesh during the year 2007. Five representative bursal samples from each farm were used for the isolation of IBDVs using 9-day-old embryonated eggs of seronegative flock of layer birds and for identification the samples were subjected to agar gel immunodiffusion test (AGIDT), immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR). Out of 35 bursal samples, IBDVs were successfully isolated from 28 (80%) samples. By AGIDT, 32 (91.4%) samples were found positive for IBDV antigen. Results of AGIDT clearly indicated that IBDVs detected in 29 bursal samples of six affected farms were identical to each other but not to IBDVs present in the remaining three samples of another farm. Indirect immunoperoxidase staining of the bursal sections revealed the presence of IBDV antigen in 32 (91.4%) samples and the IBDV antigen was detected mainly in the cortex of the lymphoid follicles of the bursal tissues. In histopathology, cell depletion, atrophy and necrosis were observed in many bursal follicles with severe edema of interfollicular septa. Of the 35 bursal samples, 34 (97.1%) samples generated 254 bp product by RT-PCR. In conclusion, the results of virus isolation and identification by AGIDT, IHC and the analysis of viral genome by RT-PCR confirmed the outbreaks of acute IBD in commercial poultry of Bangladesh. Moreover, histopathological findings and results of AGIDT gave a clear indication that the isolates from six outbreaks were different from classical strain and it seems to be of very virulent strain. On the other hand, the isolates from the other outbreak were similar to the classical strain.
Transparent and Flexible Haptic Actuator based on Cellulose Acetate Stacked Membranes
Md Mohiuddin,Hyun-U Ko,김현찬,김재환,김상연 한국정밀공학회 2015 International Journal of Precision Engineering and Vol.16 No.7
The tactile sensation is the human interact with machines in the form of felt sensations in the hand or other parts of body skin. This paper presents a film type actuator for haptic feedback devices, based on electrostatic actuation of plasticized cellulose acetate stacked membranes actuator, which is fabricated and evaluated for suitability of haptic feedback devices. The stacked membranes actuator shows many promising properties such as high transparency, lightweight, wide range of actuation frequency and high vibration acceleration for instance. The actuator structure shows intense vibration acceleration due to several layers of stacked membranes associated with the intensified electrostatic attraction force between chargeable cellulose acetate membranes. Experiment for measuring vibrational acceleration was conducted over a wide frequency range and actuation voltage to prove actuator’s great potential application as tactile actuator of haptic feedback devices. In addition, the operating principle, fabrication method and performance measurements are explained in details.
Mohiuddin, Md,Akther, Asma,Jo, Eun Byul,Kim, Hyun Chan,Kim, Jaehwan Professional Engineering Publishing Ltd 2016 Proceedings of the Institution of Mechanical Engin Vol. No.
<P>The present study investigates a film actuator made with dielectric cellulose acetate films separated by narrow spacers as a means of electrostatic actuation for potential haptic application. Fabrication process for the actuator is explained along with experiments conducted over a wide frequency range of actuation frequency. A valid finite element simulation of the actuator is made on the quarter section of the actuator by using full 3D finite elements. Vibration characteristics such as fundamental natural frequency, mode shape and output velocity in the frequency range for haptic feeling generation are obtained from the finite element analysis and compared with the experimental results. Experimental results demonstrate that the finite element model is practical and effective enough in predicting the vibration characteristics of the actuator for haptic application. The film actuator shows many promising properties like high transparency, wide range of actuation frequency and high vibration velocity for instance.</P>
Sk Mohiuddin Choudhury,Mohammad Musharraf Uddin Bhuiyan,Mohammad Moshiur Rahman,Md. Masudur Rahman,Md. Newaz Sharif,Jayonta Bhattacharjee,Farida Yeasmin Bari,Nasrin Sultana Juyena 사단법인 한국동물생명공학회 2017 한국동물생명공학회지 Vol.32 No.4
Cryopreservation of oocytes by vitrification technique may contribute a lot in the field of reproductive biotechnology. The objectives of the present study were to evaluate the effectiveness of two cryo-devices for vitrification of immature oocytes of indigenous zebu cows. Slaughter house derived immature cumulus-oocyte-complexes (COCs) of cows were vitrified using 15% dimethyl sulphoxide (DMSO) as cryoprotective agent (CPA) with 0.5 mol sucrose in TCM 199 supplemented with 20% FBS. Vitrification of COCs was completed after immediate plunging of COCs loaded cryotop or French mini straw into the liquid nitrogen (LN2). Then the COCs containing cryotop or French mini straws were warmed in 0.25 mol sucrose and 20% FBS supplemented TCM 199 followed by in vitro culture in 50 μl droplets of bicarbonate buffered TCM 199 supplemented with 10% FBS, pyruvate, FSH and oestradiol for 24 hrs at 39°C with 5% CO2 in humidified air. After maturation culture, oocytes were denuded and examined under inverted microscope for presence of polar body as the indication of maturation. Denuded oocytes were also stained by whole mount technique using 1% orcein to examine the maturation by presence of MII chromosomes. The in vitro maturation rate was significantly (p<0.05) higher in oocytes vitrified and warmed using crytop (47.1±6.9%) than that of French mini straw (15.9±12.5%). Moreover, in vitro maturation rate was significantly (p<0.05) highe r in control oocytes (not vitrified) (84.5±14.2%) than that of vitrified oocytes. In conclusion, cryotop is better than French mini straw as cryo-device for vitrification of bovine immature oocytes
Choudhury, Sk Mohiuddin,Bhuiyan, Mohammad Musharraf Uddin,Rahman, Mohammad Moshiur,Rahman, Md. Masudur,Sharif, Md. Newaz,Bhattacharjee, Jayonta,Bari, Farida Yeasmin,Juyena, Nasrin Sultana The Korean Society of Embryo Transfer 2017 한국동물생명공학회지 Vol.32 No.4
Cryopreservation of oocytes by vitrification technique may contribute a lot in the field of reproductive biotechnology. The objectives of the present study were to evaluate the effectiveness of two cryo-devices for vitrification of immature oocytes of indigenous zebu cows. Slaughter house derived immature cumulus-oocyte-complexes (COCs) of cows were vitrified using 15% dimethyl sulphoxide (DMSO) as cryoprotective agent (CPA) with 0.5 mol sucrose in TCM 199 supplemented with 20% FBS. Vitrification of COCs was completed after immediate plunging of COCs loaded cryotop or French mini straw into the liquid nitrogen ($LN_2$). Then the COCs containing cryotop or French mini straws were warmed in 0.25 mol sucrose and 20% FBS supplemented TCM 199 followed by in vitro culture in $50{\mu}l$ droplets of bicarbonate buffered TCM 199 supplemented with 10% FBS, pyruvate, FSH and oestradiol for 24 hrs at $39^{\circ}C$ with 5% CO2 in humidified air. After maturation culture, oocytes were denuded and examined under inverted microscope for presence of polar body as the indication of maturation. Denuded oocytes were also stained by whole mount technique using 1% orcein to examine the maturation by presence of MII chromosomes. The in vitro maturation rate was significantly (p<0.05) higher in oocytes vitrified and warmed using crytop ($47.1{\pm}6.9%$) than that of French mini straw ($15.9{\pm}12.5%$). Moreover, in vitro maturation rate was significantly (p<0.05) highe r in control oocytes (not vitrified) ($84.5{\pm}14.2%$) than that of vitrified oocytes. In conclusion, cryotop is better than French mini straw as cryo-device for vitrification of bovine immature oocytes.
Shaikh J. Mohiuddin,Md. Ashraful Haque,Md. Manjurul Haque,Md. Tofazzal Islam,Partha S Biswas 한국육종학회 2020 Plant Breeding and Biotechnology Vol.8 No.4
Molecular mapping and application of quantitative trait loci (QTL) associated with a higher level of grain Zinc is aviable option to enhance zinc content in rice through breeding. An F2 population derived from a cross between a high yielding variety,BRRI dhan28, and a locally adapted Zn enriched cultivar, Kalobokri was used to map QTLs associated with higher levels of Zn in ricegrain. The F2:3 progenies varied significantly (P < 0.0001) in Zinc contents with a mean value remarkably higher than those in thesuperior parent. Through marker by trait analysis using IciMapping, we detected a large-effect QTL, qGZn3 on chromosome 3 betweenRM5419 and RM1164 spanning 1.83 Mb interval at the 0.05 level of significance with a threshold LOD of 10.61. This QTL showed a21.1% (R2value) contribution to the total phenotypic variation for zinc content in the unpolished rice grains with 4.68 μg/g additiveeffect of Kalobokri alleles. We also detected 11 metal homeostasis related genes within the interval of qGZn3. In-silico analysis showedthat four expressed sequence tags of one candidate gene (LOC_Os03g22810) encoding Cu/Zn superoxide dismutase, a metal-bindingprotein, are highly active in the endosperm as well as in the embryonic tissue of rice grain. Taken together, our results suggest thatqGZn3 is a major QTL associated with high grain Zn content in the F2 progenies of rice. Our findings offer valuable genetic resourcesto facilitate breeding for high yielding and Zinc-enriched rice variety.
Ferdous, Nadim,Reza, Mahjerin Nasrin,Emon, Md. Tabassum Hossain,Islam, Md. Shariful,Mohiuddin, A.K.M.,Hossain, Mohammad Uzzal Korea Genome Organization 2020 Genomics & informatics Vol.18 No.3
Streptomyces coelicolor is a gram-positive soil bacterium which is well known for the production of several antibiotics used in various biotechnological applications. But numerous proteins from its genome are considered hypothetical proteins. Therefore, the present study aimed to reveal the functions of a hypothetical protein from the genome of S. coelicolor. Several bioinformatics tools were employed to predict the structure and function of this protein. Sequence similarity was searched through the available bioinformatics databases to find out the homologous protein. The secondary and tertiary structure were predicted and further validated with quality assessment tools. Furthermore, the active site and the interacting proteins were also explored with the utilization of CASTp and STRING server. The hypothetical protein showed the important biological activity having with two functional domain including POD-like_MBL-fold and rhodanese homology domain. The functional annotation exposed that the selected hypothetical protein could show the hydrolase activity. Furthermore, protein-protein interactions of selected hypothetical protein revealed several functional partners those have the significant role for the bacterial survival. At last, the current study depicts that the annotated hypothetical protein is linked with hydrolase activity which might be of great interest to the further research in bacterial genetics.