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The two-scale analysis method for bodies with small periodic configurations
Cui, J.Z.,Shih, T.M.,Wang, Y.L. Techno-Press 1999 Structural Engineering and Mechanics, An Int'l Jou Vol.7 No.6
The mechanical behaviours of the structure made from composite materials or the structure with periodic configurations depend not only on the macroscopic conditions of structure, but also on the detailed configurations. The Two-Scale Analysis (TSA) method for these structures, which couples the macroscopic characteristics of structure with its detailed configurations, is configurations, is presented for 2 or 3 dimensional case in this paper. And the finite element algorithms based on TSA are developed, and some results of numerical experiments are given. They show that TSA with its finite element algorithms is more effective.
Cui, M.H.,Yoo, K.S.,Hyoung, S.,Nguyen, H.T.K.,Kim, Y.Y.,Kim, H.J.,Ok, S.H.,Yoo, S.D.,Shin, J.S. North-Holland Pub ; Elsevier Science Ltd 2013 FEBS letters Vol.587 No.12
We have characterized the function of a plant R2R3-MYB transcription factor, Arabidopsis thaliana MYB20 (AtMYB20). Transgenic plants overexpressing AtMYB20 (AtMYB20-OX) enhanced salt stress tolerance while repression lines (AtMYB20-SRDX) were more vulnerable to NaCl than wild-type plants. Following NaCl treatment, the expressions of ABI1, ABI2 and AtPP2CA, which encode type 2C serine/threonine protein phosphatases (PP2Cs) that act as negative regulators in abscisic acid (ABA) signaling, were suppressed in AtMYB20-OX but induced in AtMYB20-SRDX. The electrophoretic mobility shift assay results revealed that AtMYB20 binds to the promoter regions containing the MYB recognition sequence (TAACTG) and an ACGT core element of ABI1 and AtPP2CA. These findings suggest that AtMYB20 down-regulates the expression of PP2Cs, the negative regulator of ABA signaling, and enhances salt tolerance.
Byun, M.Y.,Lee, J.,Cui, L.H.,Kang, Y.,Oh, T.K.,Park, H.,Lee, H.,Kim, W.T. Elsevier Scientific Publishers Ireland Ltd 2015 Plant science Vol.236 No.-
Deschampsia antarctica is an Antarctic hairgrass that grows on the west coast of the Antarctic peninsula. In this report, we have identified and characterized a transcription factor, D. antarctica C-repeat binding factor 7 (DaCBF7), that is a member of the monocot group V CBF homologs. The protein contains a single AP2 domain, a putative nuclear localization signal, and the typical CBF signature. DaCBF7, like other monocot group V homologs, contains a distinct polypeptide stretch composed of 43 amino acids in front of the AP2 motif. DaCBF7 was predominantly localized to nuclei and interacted with the C-repeat/dehydration responsive element (CRT/DRE) core sequence (ACCGAC) in vitro. DaCBF7 was induced by abiotic stresses, including drought, cold, and salinity. To investigate its possible cellular role in cold tolerance, a transgenic rice system was employed. DaCBF7-overexpressing transgenic rice plants (Ubi:DaCBF7) exhibited markedly increased tolerance to cold stress compared to wild-type plants without growth defects; however, overexpression of DaCBF7 exerted little effect on tolerance to drought or salt stress. Transcriptome analysis of a Ubi:DaCBF7 transgenic line revealed 13 genes that were up-regulated in DaCBF7-overexpressing plants compared to wild-type plants in the absence of cold stress and in short- or long-term cold stress. Five of these genes, dehydrin, remorin, Os03g63870, Os11g34790, and Os10g22630, contained putative CRT/DRE or low-temperature responsive elements in their promoter regions. These results suggest that overexpression of DaCBF7 directly and indirectly induces diverse genes in transgenic rice plants and confers enhanced tolerance to cold stress.
Cytotoxicity and Cells Growth Status on Ag+-implanted Pyrolytic Carbon and TiN/Ag Multilayer Films
X. M. Cai,H. Q. Tang,T. Liu,J. Zhao,H. Q. Gu,R. Z. Cui 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.-
Ag+-implanted pyrolytic carbon and TiN/Ag multilayer films were prepared by ion implantation and ion beam assisted deposition. L-929 cells (mice fibroblast) were cultured in the extracted medium of Ag+-implanted pyrolytic carbon and TiN/Ag multilayer film samples for cytotoxicity testing. The adhesion, spreading, proliferation and morphology of human umbilical vein endothelial cells (HUVEC) on both samples were also investigated. The results show that the cytotoxicity grade of Ag+-implanted pyrolytic carbon is less than 1˚ when the implanted dose is under 1×10 16 ions/cm² and the cytotoxicity grade of TiN/Ag multilayer films with modulation period of 7.5 nm is within the range of 0 to 1˚ indicating that both samples have no cytotoxicity to L-929. HUVEC on both Ag+-implanted pyrolytic carbon and TiN/Ag multilayer film sample grows well, showing that they have good biocompatibility. Ag+-implanted pyrolytic carbon and TiN/Ag multilayer films were prepared by ion implantation and ion beam assisted deposition. L-929 cells (mice fibroblast) were cultured in the extracted medium of Ag+-implanted pyrolytic carbon and TiN/Ag multilayer film samples for cytotoxicity testing. The adhesion, spreading, proliferation and morphology of human umbilical vein endothelial cells (HUVEC) on both samples were also investigated. The results show that the cytotoxicity grade of Ag+-implanted pyrolytic carbon is less than 1˚ when the implanted dose is under 1×10 16 ions/cm² and the cytotoxicity grade of TiN/Ag multilayer films with modulation period of 7.5 nm is within the range of 0 to 1˚ indicating that both samples have no cytotoxicity to L-929. HUVEC on both Ag+-implanted pyrolytic carbon and TiN/Ag multilayer film sample grows well, showing that they have good biocompatibility.
Kim, J.K.,Cui, C.H.,Yoon, M.H.,Kim, S.C.,Im, W.T. Elsevier Science Publishers 2012 Journal of biotechnology Vol.161 No.3
This study focuses on the cloning, expression, and characterization of recombinant ginsenoside hydrolyzing glycosidase from Sanguibacter keddieii in order to biotransform ginsenosides efficiently. The gene, termed bglSk, consists of 1857bp and revealed significant homology to that of glycoside hydrolase family 3. The enzyme was over-expressed in Escherichia coli BL21 (DE3) using a GST-fused pGEX 4T-1 vector system. The over-expressed recombinant enzymes could convert six major ginsenosides Rb<SUB>1</SUB>, Rb<SUB>2</SUB>, Rc, Rd, Re and Rg<SUB>1</SUB> into more pharmacologically active rare ginsenosides such as C-Y, C-Mc, C-K, Rg<SUB>2</SUB>(S), and F<SUB>1</SUB>. Especially, BglSk could completely convert the Rg<SUB>1</SUB> into F<SUB>1</SUB>. The GST-fused BglSk was purified with GST.bind agarose resin and then characterized. The kinetic parameters for β-glucosidase had apparent K<SUB>m</SUB> values of 0.456+/-0.009 and 0.167+/-0.003mM and V<SUB>max</SUB> values of 30.2+/-0.7 and 4.1+/-0.1μmolmin<SUP>-1</SUP>mg of protein<SUP>-1</SUP> against p-nitrophenyl-β-d-glucopyranoside and Rb<SUB>1</SUB>, respectively.
Variable number of tandem repeats of 9 Plasmodium vivax genes among Southeast Asian isolates
Wang, B.,Nyunt, M.H.,Yun, S.G.,Lu, F.,Cheng, Y.,Han, J.H.,Ha, K.S.,Park, W.S.,Hong, S.H.,Lim, C.S.,Cao, J.,Sattabongkot, J.,Kyaw, M.P.,Cui, L.,Han, E.T. Verlag fur Recht und Gesellschaft 2017 Acta tropica Vol.170 No.-
<P>The variable number of tandem repeats (VNTRs) provides valuable information about both the functional and evolutionary aspects of genetic diversity. Comparative analysis of 3 Plasmodium falciparum genomes has shown that more than 9% of its open reading frames (ORFs) harbor VNTRs. Although microsatellites and VNTR genes of P. vivax were reported, the VNTR polymorphism of genes has not been examined widely. In this study, 230 P. vivax genes were analyzed for VNTRs by SERV, and 33 kinds of TR deletions or insertions from 29P. vivax genes (12.6%) were found. Of these, 9 VNTR fragments from 8 P. vivax genes were used for PCR amplification and sequence analysis to examine the genetic diversity among 134 isolates from four Southeast Asian countries (China, Republic of Korea, Thailand, and Myanmar) with different malaria endemicity. We confirmed the existence of extensive polymorphism of VNTR fragments in field isolates. This detection provides several suitable markers for analysis of the molecular epidemiology of P. vivax field isolates. (C) 2017 Elsevier B.V. All rights reserved.</P>