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소 뇌막으로부터 5'-Nucleotidase의 효소적 용출에 대한 내재계면활성제의 효과
류희문,석대은 충남대학교 약학대학 의약품개발연구소 2000 藥學論文集 Vol.16 No.-
Glycosylphosphatidylinositol (GPI)-linked 5'-nucleotidase is released as both amphiphilic form (Amp-nucleotidase) and hydrophilic form (Hyd-nucleotidase) from bovine brain membrane. Exposure of brain membrane to deoxycholate, lysolecithin or monooleoylglycerol leads to a concentration-dependent release of Hyd-nucleotidase with deoxycholate being the most effective. Next, the brain GPI-PLD-catalyzed conversion of Amp-nucleotidase to Hyd-nucleotidase was investigated. The GPI-PLD-catalyzed conversion of required detergents, and among detergents used, monooleoylglycerol was the most effective for the enzymatic conversion. Monooleoylglycerol exhibited a concentration-dependent effect on GPI-PLD activity up to 0.3 mM, but its effect decreased at 1 mM. In addition, the combinational effect of monooleoylglycerol and lysolecithin on GPI-PLD action was not significant. Based on these results. it is suggested that the formation of Hyd-nucleotidase in brain tissue may be ascribed to the activities of GPI-PLC and GPI-PLD in the presence of bio-detergent.
합성품 Peptide와의 비교실험을 통한 Bovine Thyroglobulin의 50K Polypeptide내에 존재하는 Diiodotyrosine-1375의 구조동정
류희문,석대은 충남대학교 약학대학 의약품개발연구소 2000 藥學論文集 Vol.16 No.-
A peptide fragment containing residues 1218-1591, prepared from thermolysin-mediated proteolysis of bovine thyroglobulin, was reduced by dithiothreitol and then treated with iodoacetic acid. The carboxymethylated peptide was digested with endoproteinase Asp-N, and fractionated by RP-HPLC. The fractions were analyzed by LC/ESI-MS for the monitor of a peptide with a hormonogenic site at Tyr-1375, and a fraction was found to contain to a peptide (residues 1366-1381) containing Tyr-1375. This observation was positively confirmed by the comparison with synthetic peptide, DVEEALAGK (diiodotyrosine) LAGRFA, which was produced from the oxidative iodination of DVEEALAGKYLAGRFA by lactoperoxidase employing KI and (H_2)(O_2)-generating system.
Wang Tingting,Yang Yang,Liu Xinyue,Deng Jiajun,Wu Junqi,Hou Likun,Wu Chunyan,She Yunlang,Sun Xiwen,Xie Dong,Chen Chang 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.4
Objective: To investigate the association between CT imaging features and survival outcomes in patients with primary invasive mucinous adenocarcinoma (IMA). Materials and Methods: Preoperative CT image findings were consecutively evaluated in 317 patients with resected IMA from January 2011 to December 2015. The association between CT features and long-term survival were assessed by univariate analysis. The independent prognostic factors were identified by the multivariate Cox regression analyses. The survival comparison of IMA patients was investigated using the Kaplan-Meier method and propensity scores. Furthermore, the prognostic impact of CT features was assessed based on different imaging subtypes, and the results were adjusted using the Bonferroni method. Results: The median follow-up time was 52.8 months; the 5-year disease-free survival (DFS) and overall survival rates of resected IMAs were 68.5% and 77.6%, respectively. The univariate analyses of all IMA patients demonstrated that 15 CT imaging features, in addition to the clinicopathologic characteristics, significantly correlated with the recurrence or death of IMA patients. The multivariable analysis revealed that five of them, including imaging subtype (p = 0.002), spiculation (p < 0.001), tumor density (p = 0.008), air bronchogram (p < 0.001), emphysema (p < 0.001), and location (p = 0.029) were independent prognostic factors. The subgroup analysis demonstrated that pneumonic-type IMA had a significantly worse prognosis than solitary-type IMA. Moreover, for solitary-type IMAs, the most independent CT imaging biomarkers were air bronchogram and emphysema with an adjusted p value less than 0.05; for pneumonic-type IMA, the tumors with mixed consolidation and ground-glass opacity were associated with a longer DFS (adjusted p = 0.012). Conclusion: CT imaging features characteristic of IMA may provide prognostic information and individual risk assessment in addition to the recognized clinical predictors.
Xu Zhiyang,Zhou Kaixiang,Wang Zhenni,Liu Yang,Wang Xingguo,Gao Tian,Xie Fanfan,Yuan Qing,Gu Xiwen,Liu Shujuan,Xing Jinliang 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Ovarian cancer (OC) is the most lethal gynecologic tumor and is characterized by a high rate of metastasis. Challenges in accurately delineating the metastatic pattern have greatly restricted the improvement of treatment in OC patients. An increasing number of studies have leveraged mitochondrial DNA (mtDNA) mutations as efficient lineage-tracing markers of tumor clonality. We applied multiregional sampling and high-depth mtDNA sequencing to determine the metastatic patterns in advanced-stage OC patients. Somatic mtDNA mutations were profiled from a total of 195 primary and 200 metastatic tumor tissue samples from 35 OC patients. Our results revealed remarkable sample-level and patient-level heterogeneity. In addition, distinct mtDNA mutational patterns were observed between primary and metastatic OC tissues. Further analysis identified the different mutational spectra between shared and private mutations among primary and metastatic OC tissues. Analysis of the clonality index calculated based on mtDNA mutations supported a monoclonal tumor origin in 14 of 16 patients with bilateral ovarian cancers. Notably, mtDNA-based spatial phylogenetic analysis revealed distinct patterns of OC metastasis, in which a linear metastatic pattern exhibited a low degree of mtDNA mutation heterogeneity and a short evolutionary distance, whereas a parallel metastatic pattern showed the opposite trend. Moreover, a mtDNA-based tumor evolutionary score (MTEs) related to different metastatic patterns was defined. Our data showed that patients with different MTESs responded differently to combined debulking surgery and chemotherapy. Finally, we observed that tumor-derived mtDNA mutations were more likely to be detected in ascitic fluid than in plasma samples. Our study presents an explicit view of the OC metastatic pattern, which sheds light on efficient treatment for OC patients.