The motion rule of sand particles under control of the sand transportation engineering

Lin-gui Xin,Jian-jun Cheng,Bo-yu Chen,Rui Wang 한국풍공학회 2018 Wind and Structures, An International Journal (WAS Vol.27 No.4

In the desert and Gobi regions with strong wind and large sediment discharge, sand transporting engineering is more effective than sand blocking and sand fixing measures in sand prevention. This study uses the discrete phase model of 3D numerical simulation to study the motion trail, motion state and distribution rule of sand particles with different grain diameters when the included angle between the main shaft of the feather-row lateral transportation sand barrier and the wind direction changes, and conducts a comparison in combination with the wind tunnel test and the flow field rule of common sand barrier. According to the comparison, when wind-sand incoming flow passes through a feather-row sand barrier, sand particles slow down and deposit within the deceleration area under the resistance of the feather-row sand barrier, move along the transportation area formed by the transportation force, and accumulate as a ridge at the tail of the engineering. With increasing wind speed, the eolian erosion of the sand particles to the ground and the feather-row sand barrier is enhanced, and the sand transporting quantity and throw-over quantity of the feather-row sand barrier are both increased. When sand particles with different grain diameters bypass the feather-row sand barrier, the particle size of the infiltrating sands will increase with the included angle between the main shaft of the feather-row sand barrier and the wind direction. The obtained result demonstrates that, at a constant wind speed, the flow field formed is most suitable for the lateral transportation of the wind-drift flow when the included angle between the main shaft of the feather-row sand barrier lateral transportation engineering and the wind speed is less than or equal to 30°.

Scutellaria Extract Decreases the Proportion of Side Population Cells in a Myeloma Cell Line by Down-regulating the Expression of ABCG2 Protein

Lin, Mei-Gui,Liu, Li-Ping,Li, Chen-Yin,Zhang, Meng,Chen, Yuling,Qin, Jian,Gu, Yue-Yu,Li, Zhi,Wu, Xin-Lin,Mo, Sui-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Background and Aims: Scutellaria is one of the most popular traditional Chinese herbal remedies against various human diseases, including cancer. In this study, we examined the active effects of Scutellaria extract and its main flavonoid constituents on the proportion of side population cells within human multiple myeloma cell line RPMI8226 in vitro and explored the potential molecular mechanisms involved. Materials and Methods: The contents of flavonoids in ethanolic extract of Scutellaria baicalensis Georgi were determined using high performance liquid chromatography. The antiproliferative effect of the ethanolic extract on RPMI-8226 was determined by CCK assay. Apoptosis was measured by annexin combining with propidium iodide in a flow cytometer. Cell cycle analysis was performed by propidium iodide staining in combination with flow cytometry analysis. Hoechst 33342 exclusion assay was used for the identification of side population within RPMI8226 cells. The expression of ABCG2 protein was assessed by Western blotting assay. Results: The content of major flavonoids constitutents of Scutellaria extract was baicalin (10.2%), wogonoside (2.50%), baicalein (2.29%), and wogonin (0.99%), respectively. The crude Scutellaria extract did not show significant anti-proliferative effect, apoptosis induction and cell cycle arrest in RPMI-8226 within the concentrations of $1-75{\mu}g/mL$. However, the ethanolic extract, baicalein, wogonin and baicalin reduced the side population cells in RPMI-8226, and data showed that baicalein and wogonin had stronger inhibitory effects. Correspondingly, they also exhibited significant effects on decreasing the expression level of ABCG2 protein in RPMI-8226 in vitro. Conclusions: Our results for the first time demonstrated a novel mechanism of action for Scutellaria extract and its main active flavonoids, namely targeting SP cells by modulating the expression of ABCG2 protein. This study provides an insight for new therapeutic strategies targeting cancer stem cells of multiple myeloma.

Triterpenoid Saponins from the Seeds of Caragana microphylla

Gui-Lin Jin,Cheng-Jian Zheng,Wen-Bo Xin,Zhu-Jun Mao,Pei-Xin Sun,Zhi-Xin Zeng,Lu-Ping Qin 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.6

Two new triterpenoid saponins, namely caraganoside C (1) and caraganoside D (2), were isolated from the seeds of Caragana microphylla. Their structures were elucidated on the basis of spectroscopic analyses, including homo- and hetero-nuclear correlation NMR experiments (COSY, HSQC and HMBC). Both 1 and 2 exhibited moderate inhibitory activity against NO production in LPS-stimulated RAW264.7 cells with IC_50 values of 26.4 μM and 32.2 μM, respectively. In addition, 1 showed weak cytotoxicity against MCF-7, HL-60, HCT116, and A549 cell lines.

The motion rule of sand particles under control of the sand transportation engineering

Xin, Lin-gui,Cheng, Jian-jun,Chen, Bo-yu,Wang, Rui Techno-Press 2018 Wind and Structures, An International Journal (WAS Vol.27 No.4

In the desert and Gobi regions with strong wind and large sediment discharge, sand transporting engineering is more effective than sand blocking and sand fixing measures in sand prevention. This study uses the discrete phase model of 3D numerical simulation to study the motion trail, motion state and distribution rule of sand particles with different grain diameters when the included angle between the main shaft of the feather-row lateral transportation sand barrier and the wind direction changes, and conducts a comparison in combination with the wind tunnel test and the flow field rule of common sand barrier. According to the comparison, when wind-sand incoming flow passes through a feather-row sand barrier, sand particles slow down and deposit within the deceleration area under the resistance of the feather-row sand barrier, move along the transportation area formed by the transportation force, and accumulate as a ridge at the tail of the engineering. With increasing wind speed, the eolian erosion of the sand particles to the ground and the feather-row sand barrier is enhanced, and the sand transporting quantity and throw-over quantity of the feather-row sand barrier are both increased. When sand particles with different grain diameters bypass the feather-row sand barrier, the particle size of the infiltrating sands will increase with the included angle between the main shaft of the feather-row sand barrier and the wind direction. The obtained result demonstrates that, at a constant wind speed, the flow field formed is most suitable for the lateral transportation of the wind-drift flow when the included angle between the main shaft of the feather-row sand barrier lateral transportation engineering and the wind speed is less than or equal to $30^{\circ}$.

Preventive Effect of Polysaccharide of Larimichthys crocea Swim Bladder on Reserpine Induced Gastric Ulcer in ICR Mice

Li, Gui-Jie,Sun, Peng,Wang, Rui,Zhou, Ya-Lin,Qian, Yu,Zhao, Xin The Korean Society of Pharmacology 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.2

This project's aim was to determine the reserpine-induced gastric ulcer preventive effect of polysaccharide of Larimichthys crocea swim bladder (PLCSB) in ICR mice. The anti-gastric ulcer effects of polysaccharide of Larimichthys crocea swim bladder was evaluated in mice model using morphological test, serum levels assay, cytokine levels assay, tissue contents analysis, reverse transcription-polymerase chain reaction (RT-PCR) analysis and western bolt assay. High concentration (50 mg/kg dose) of PLCSB reduced IFN-${\gamma}$ as compared to low concentration (25 mg/kg dose) and control mice. SS and VIP serum levels of PLCSB treated mice were higher than those of control mice, and MOT and SP serum levels were lower than control mice. Gastric ulcer inhibitory index of PLCSB treatment groups mice were much lower than control mice, and the high concentration treated mice were similar to the ranitidine treated mice. The SOD and GSH-Px activities of PLCSB treated mice were higher than control mice, close to normal mice and ranitidine treated mice. PLCSB treated mice also showed the similar contents of NO and MDA to normal group. By RT-PCR and western blot assay, PLCSB significantly induced inflammation in tissues of mice by downregulating NF-${\kappa}B$, iNOS, and COX-2, and upregulating $I{\kappa}B-{\alpha}$. These results suggest that PLCSB showed a good gastric ulcer preventive effect as the gastric ulcer drug of ranitidine. Polysaccharide of Larimichthys crocea swim bladder may be used as a drug material from marine products.

Biodegradable Poly(lactic-co-glycolic acid) Maxpol-T/S as Novel Scaffold for Adipose Derived Stem Cells and Fibroblast Growth In Vitro

( Zhe Jin ),( Yan Qing Gong ),( Xin Cheng Qin ),( Jian Zhang ),( Yi Chen Zhu ),( Gui Ting Lin ),( Tom F Lue ),( Zhong Cheng Xin ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4

To explore the ability of MaxPol-T/S, a novelbiodegradable poly-lactic-co-glycolic acid(PLGA) scaffold, in providing a cell-matrix interaction interface for cell growth, we generated GFP positive adipose derived stem cell(ADSCGFP+) and fibroblasts(FCGFP+) for investigating the cellular growth on this biomaterial. The MaxPol-T/S was produced through salt-leaching/particulate-leaching technology for tissue engineering, which provides a modified surface for the best cellular attachment sites and nutrient supply and waste exchange conditions. The morphological features of MaxPol-T/S were studied with a scanning electron microscope and the ADSCGFP+ and FCGFP+ were confirmed by auto-fluorescence microscopy and flow cytometry assay. Being seeded onto the MaxPol-T/S in vitro, the growth and morphology of ADSCGFP+ and FCGFP+ were further verified by auto-fluorescence microscopy and MMT test. The ADSCs and fibroblasts, expressed strong GFP signals in the cytoplasm and nucleus, adhered and proliferated on the surface of scaffold MaxPol-T/S. Both cell lines survived on the scaffold more than 21 days in vitro and formed three-dimensional colonies on the surface of the MaxPol-T. In conclusion, MaxPol-T/S is a novel PLGA scaffold for ADSCGFP+ and FCGFP+ and implies a promising technique for tissue engineering.

On snap-buckling of FG-CNTR curved nanobeams considering surface effects

Yuan Yuan Zhang,Yu X. Wang,Xin Zhang,Huo M. Shen,Gui-Lin She 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.38 No.3

The aim of this paper is to analyze the nonlinear bending of functionally graded (FG) curved nanobeams reinforced by carbon nanotubes (CNTs) in thermal environment. Chen-Yao’s surface elastic theory and geometric nonlinearity are also considered. The nanobeams are subjected to uniform loadings and placed on three-parameter substrates. The Euler-Lagrange equations are employed to deduce the equations of equilibrium. Then, the asymptotic solutions and boundary value problems are analytically determined by utilizing the two-step perturbation technique. Finally, the effects of the surface parameters, geometric factors, foundation stiffness, volume fraction, thermal effects and layout type of CNTs on the nonlinear bending of the nanobeams are discussed.

UHRF2 mRNA Expression is Low in Malignant Glioma but Silencing Inhibits the Growth of U251 Glioma Cells in vitro

Wu, Ting-Feng,Zhang, Wei,Su, Zuo-Peng,Chen, San-Song,Chen, Gui-Lin,Wei, Yong-Xin,Sun, Ting,Xie, Xue-Shun,Li, Bin,Zhou, You-Xin,Du, Zi-Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

UHRF2 is a member of the ubiquitin plant homeo domain RING finger family, which has been proven to be frequently up-regulated in colorectal cancer cells and play a role as an oncogene in breast cancer cells. However, the role of UHRF2 in glioma cells remains unclear. In this study, we performed real-time quantitative PCR on 32 pathologically confirmed glioma samples (grade I, 4 cases; grade II, 11 cases; grade III, 10 cases; and grade IV, 7 cases; according to the 2007 WHO classification system) and four glioma cell lines (A172, U251, U373, and U87). The expression of UHRF2 mRNA was significantly lower in the grade III and grade IV groups compared with the noncancerous brain tissue group, whereas its expression was high in A172, U251, and U373 glioma cell lines. An in vitro assay was performed to investigate the functions of UHRF2. Using a lentivirus-based RNA interference (RNAi) approach, we down-regulated UHRF2 expression in the U251 glioma cell line. This down-regulation led to the inhibition of cell proliferation, an increase in cell apoptosis, and a change of cell cycle distribution, in which S stage cells decreased and G2/M stage cells increased. Our results suggest that UHRF2 may be closely related to tumorigenesis and the development of gliomas.