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임용석,마선미,나성태,최홍근,신현철 한국육수학회 2005 생태와 환경 Vol.38 No.1
팔당호 연안대의 수생식물상과 식생을 파악하기 위하여 2003년 4월부터 2004년 4월까지 9곳의 조사 지점을 선정하여 선방형구법을 이용하여 조사하였다. 팔당호 연안대에는 128종류의 관속식물이 생육하고 있는 것으로 조사되었고, 이중 수생식물은 38종류였다. 수생식물의 경우 정수식물이 21종류로 가장 많았고, 침수식물은 8종류 였고, 침수식물 종류수는 이전 조사보다 감소한 것으로 파악되었다. 팔당호 주요 식생은 애기부들, 줄, 갈대가 우점하는 정수식물대로 파악되었고, 특히 두물머리, 광동교 근처에 넓게 정수식물대가 넓게 발달하였다. 귀화식물은 11종류가 분포하고 있었으나, 출현 빈도는 상대적으로 높았다. 팔당호 연안대에 분포하는 식물의 출현 빈도를 조사 지점을 기준으로 살펴보면, 조사 지점당 수생식물은 2.7종류, 습생식물은 2.5종류, 육지식물은 1.8종류로 파악 되었고, 수직대상분포 식생이 팔당호 연안대에서 발견되었다. 이러한 조사 결과는 팔당호 연안대가 수생태계의 전형적인 특성들을 지니고 있는 것으로 풀이된다. To investigate the flora and vegetation structure of vascular plants in the littoral zone of Paldang Reservoir, from April, 2003 to April 2004, nine sampling sites were selected. Along the Paldang Reservoir, 128 taxa, consisted of 51 families and 96 genera, were identified, among them, hydrophytes were confirmed as 38 taxa, which was comprised 29.7% to total taxa, whereas hygrophytes were 44 taxa and terrestrial plants were 46 taxa. Emergent hydrophytes consists of 21 taxa, including Phragmites australis and Typha angustifolia, and next, submerged hydrophytes were 8 taxa. However, the kinds and vegetation area of submerged hydorphytes were reduced compared to previous studies. In the littoral zone of Paldang Reservoir, the aquatic vegetation was widely developed near Dumulmori, Yangsuri, and Kwangdong Bridge, downstream of Kyungancheon. The average number of hydrophyte per sampling sites were 2.7 taxa, whereas hygrophytes were 2.5, and land plants were 1.8. In addition, the hydrophytes in the littoral zone of Paldang Reservoir showed the typical vertical zonation pattern like a natural swamp. These results mean that the littoral zone of Paldang Reservoir has the typical characteristics of aquatic plant ecosystem.
Lim Seong-Keun,Lim Seong-Keun,Hong Soo-Min,Suh Sang Jae,Lee Seung-Yeol,Jung Hee-Young 한국균학회 2023 Mycobiology Vol.51 No.2
The fungal strain belonging to the genus Monochaetia of the family Sporocadaceae was isolated from hairy long-horned toad beetle (Moechotypa diphysis) during the screening of microfungi associated with insects from Gangwon Province, Korea. The strain KNUF-6L2F produced white, light brown to dirty black surface, and olivaceous green colonies with the higher growth, while the closest strain M. ilicis KUMCC 15–0520T were light brown to brown, and M. schimae SAUCC 212201T light brown to brown toward center. The strain KNUF-6L2F produced shorter (5.7–14.0 μm) apical appendages than M. ilicis (6.0–24.0 μm), but similar to M. schimae (7.0–12.5 μm). Three median cells of KNUF-6L2F were light brown to olivaceous green, whereas brown and olivaceous cells were observed from M. ilicis and M. schimae, respectively. And the strain KNUF-6L2F produced larger conidiogenous cells than M. ilicis and M. schimae. Additionally, phylogenetic analyses based on molecular datasets of internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (TEF1α), and β-tubulin (TUB2) genes corroborated the strain’s originality. Thus, the strain is different from other known Monochaetia species, according to molecular phylogeny and morophology, hence we suggested the new species Monochaetia mediana sp. nov. and provided a descriptive illustration.
Improved Tools for Proteome Application of Basic Seed Proteins in Soybean (Glycine max L. Merr.)
Seong-Woo Cho,Hye-Lim Kim,Keun-Yook Chung,Hong-Sig Kim,Sun-Hee Woo 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
We improved the separation of the basic proteins from the soybean cotyledon, Glycine max L. Merr. by searching N-terminal sequences data in proteins isolated by two-dimensional electrophoresis (2-DE). After removed Hexane, proteins were extracted from cotyledon with a urea/Triton/2-mercaptoetanol solution. Using this method, the highly reproducible isoelectric focusing (IEF) can formed with polyacrylamide gels with pH 4.0-9.8. The IEF tube gels were used as the first dimension, and proteins were visualized by second-dimensional gel electrophoresis, and identify a number of soybean cotyledon proteins using mass spectrometry in the proteome analysis. These instruments of 2-DE and IEF tube gels were used 27 cm and investigate under various conditions. The total number of spots and features was obtained by PDQuest software (Bio-Rad). In this experiments performed, the IEF tube gels and instruments afforded good reproducibility in the number of PDQuest-detected spots from gel to gel while IPG offered better reproducibility in the total number of manually detected spots from gel to gel. In conclusion, we have separated of the basic 13 proteins in soybean. The glycinin subunit separations are also considered to play important roles in soybean breeding and biochemical characterization. The improved technique will be useful to dissect the genetic control of glycinin expression in soybean.
Seong-Su Kim,Byung-Hyun Choi,Kyeong-Lim Lee,Jong-In Jin,Tae-Kwang Suh,Cheol-Ho Son,Chan-Ho Park,Seung-Oh Shin,Kwang-Jin Han,Hyun-Tae Lim,Kyu-Woan Cho,Il-Keun Kong 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.2
This study was designed to evaluate the possibility of increase through dairy female offspring’s ratio by transfer of pre-selected transferrable blastocyst that was produced by pre-selected X-bearing semen with OPU derived oocytes. Elite dairy female cow is demanded strongly compared with male, the so called, farmer wants to produce only an elite female dairy offspring as a candidate female dairy cow for producing milk. In our study, we selected 2 elite dairy bull semen from National Agricultural Cooperative Federation to pre-select X-bearing semen and 5 elite dairy female cows as donor for collecting of OPU derived oocytes. OPU derived embryo production system was carried out an aspiration of immature oocytes from 5 donor cows 2 times per week, total 200 times for 2 to 7 months by an ultrasonographic guided follicular aspiration system and then produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture. Dairy donor semen selected H-319, 320 bull in National Agricultural Cooperative federation was sorted X-bearing semen by flow-cytometer and frozen for using IVF with OPU derived oocytes. Donor cows were selected 5 elite dairy cows from Gyeongju Dairy Cow Community and then disease tests such as 4 kinds of disease before selecting was checked. Oocyte proportion of grade 1 to 3 from total collected oocytes was significantly lower in donor A and B than those in donor C, D and E (82.16 and 70.03% vs. 90.0, 91.78 and 93.57%), respectively (p<0.05). However, number of oocytes per session in donor A, C and E was significantly higher than those in donor B and D (7.77 ± 3.26, 5.85 ± 2.10 and 7.03 ± 2.14 vs. 4.68 ± 2.61 and 5.21 ± 1.97 oocytes), but donor A was significantly higher than donor C (p<0.05). Development to blastocyst in donor B, C and E was significantly higher than those in donor A and D (31.0, 25.0 and 25.0% vs. 14.3 and 4.5%), but donor A was not different in donor C and E (p<0.05). Nine out of 10 blastocysts (90.0%) derived from OPU blastocysts were confirmed male embryos that was induced with Y-bearing semen to confirm sex ratio only. Total 96 blastocysts derived from female bearing semen were transferred into synchronized recipients and then confirmed 42 recipients (43.8%) pregnancy rate, 36 offspring (37.5%) and 91.7% female sex ratio (33 female vs. 3 male offspring). Taken together all data, elite dairy female offspring could be produced effectively by in vitro production system between pre-selected x-bearing semen and OPU derived oocytes that would be influential breeder in the breeding of dairy farm to increase effectively elite dairy offspring ratio as well as net income in the dairy farmer.
Seong-Keun Lim,Leonid N. Ten,Diane Avalos-Ruiz,류정주,In-Kyu Kang,Seung-Yeol Lee,Hee-Young Jung 한국균학회 2022 韓國菌學會誌 Vol.50 No.3
Two fungal strains, designated KNUF-21-006 and KNUF-21-028, were isolated from soil samples collected from Gyeongbuk Province, Korea. The strain KNUF-21-006 was similar to other Pestalotiopsis species in terms of morphological characteristics, including whitish to pale brown mycelium, conidial shape, and size. The isolate had aerial hyphae that produced black fruiting bodies on the mycelium. The conidia were fusoid to ellipsoid, four-septate, and appendage-bearing. Phylogenetic analysis using the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF), and β-tubulin (TUB) gene sequences confirmed that the closest relationship of the isolate at the species level was with Pestalotiopsis clavata. The strain KNUF-21-028 exhibits similar morphological characteristics to other Botryotrichum species, including white aerial mycelium with sulcate and irregular margins, conidial shape, and size. The conidia were globose, single, and hyaline. Upon molecular analysis—using the ITS region, large subunit (LSU) rRNA gene, and TUB gene sequences—the fungus was identified as Botryotrichum iranicum. This is the first record of these fungal species in Korea.
보령시와 서천군 주민의 대중관광 및 대안관광 평가에 따른 인식 연구
임근욱(Keun Uk Lim),최성환(Seong Hwan Choi),정동근(Dong Keun Chung) 한국호텔외식관광경영학회 2013 호텔경영학연구 Vol.22 No.5
The purpose of this study was to analyze the residents` attitude of mass-tourism and alternative tourism, using data collected from residents of Boryeong and Seocheon, Chungcheongnam-Do. The study findings reveal that community support is affected directly and indirectly by the community concern, attachment, environmental attitudes, and the perceived positive or negative impacts of tourism development. The more attached residents are to the community, the more positively they perceive alternative tourism. Residents who are highly concerned about their community and community issues are more likely to be concerned about the all tourism development, but to minimize the negative consequences of development, locals are likely to support the form of development that optimizes positive impacts while minimizing negative impacts. Level of environmental attitudes of locals was found to have direct significant impact on perception of the negative impacts of tourism development. residents with low level of environmental attitudes are likely to support mass tourism development. Overall, the results of this study suggest that locals see tourism development as either good or bad.