Cucumber Growth and Nitrogen Uptake as Affected by Solution Temperature and NO₃^-:NH₄⁺ Ratios during the Seedling

Yan, Qiu-Yan,Duan, Zeng-Qiang,Li, Jun-Hui,Li, Xun,Dong, Jin-Long Korean Society of Horticultural Science 2013 원예과학기술지 Vol.31 No.4

The effect of solution temperature and nitrogen form on cucumber (Cucumis sativus L.) growth, photosynthesis and nitrogen metabolism was investigated in hydroponic culture. Cucumber plants were grown for 35 days in a greenhouse at three constant solution temperatures ($15^{\circ}C$, $20^{\circ}C$, and $25^{\circ}C$) within a natural aerial temperature ($15-30^{\circ}C$). Four nitrate:ammonium ($NO{_3}^-:NH{_4}^+$) ratios (10:0, 8:2, 5:5, and 2:8 $mmol{\cdot}L^{-1}$) at constant nitrogen (N) concentration of $10mmol{\cdot}L^{-1}$ were applied within each solution temperature treatment. Results showed an increasing solution temperature enhanced plant growth (height, dry weight, and leaf area) in most N treatments. Dry weight accumulation was greatest at the 10:0 $NO{_3}^-:NH{_4}^+$ ratio in the $15^{\circ}C$ solution, the 5:5 ratio in the $20^{\circ}C$ solution and the 8:2 ratio in the $25^{\circ}C$ solution. Photosynthetic rate (Pn) response to solution temperature and $NO{_3}^-:NH{_4}^+$ ratio was similar to that of plant growth. Probably, the photosynthate shortage played a role in the reduced biomass formation. Increasing solution temperature enhanced the nitrate reductase (NR) activity, and further reduced shoots nitrate content. Our results indicate that the optimal ratio of nitrate to ammonium that promotes growth in hydroponic cucumber varies with solution temperature.

On the Potential of Limitation-oriented Malware Detection and Prevention Techniques on Mobile Phones

Qiang Yan,Robert H. Deng,Yingjiu Li,Tieyan Li 보안공학연구지원센터 2010 International Journal of Security and Its Applicat Vol.4 No.1

Analysis of the Relationship between MHC-DRB1 Gene Polymorphism and Hydatidosis in Kazakh Sheep

Li, Ren-Yan,Jia, Bin,Zhang, Wen-Ju,Zhao, Zong-Sheng,Shi, Guo-Qing,Shen, Hong,Peng, Qiang,Lv, Li-Min,Zhou, Qi-Wei,Du, Ying-Chun Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.9

The objective of this work was to analyze the relationship between ovine major histocompatibility complex (MHC) DRB1 gene polymorphism and genetic resistance to hydatidosis in Kazakh sheep. The Ovar (ovine MHC) class II DRB1 second exon was amplified by polymerase chain reaction (PCR) from DNA samples of 702 Kazakh sheep, including 302 sheep with hydatidosis and 400 health controls. PCR products were characterized by the restriction fragment length polymorphism (RFLP) technique using five restriction enzymes, i.e., MvaI, HaeIII, SacI, SacII and Hin1I, yielding 14 alleles and 28 genotypes. Comparing the frequency of genotypes in hydatidosis sheep with the control group, it was found that the genotype frequencies of MvaIbc, Hin1Iab, SacIIab, HaeIIIde, HaeIIIdf and HaeIIIdd in control sheep were significantly (p<0.01) higher than in hydatidosis sheep, indicating that a significant correlation existed between these genotypes and resistance to hydatidosis. Genotype frequencies of MvaIbb, SacIIaa, Hin1Ibb and HaeIIIef in sheep with hydatidosis were extremely significantly (p<0.01) higher than in the control group, and the genotype frequency of HaeIIIab was significantly higher (p<0.05), indicating that a marked correlation existed between these genotypes and susceptibility to hydatidosis. By way of analyzing haplotype with these resistant genotypes, the hydatidosis resistant haplotype MvaIbc-SacIIab-Hin1Iab of Kazakh sheep was screened out, and then verified through artificial hydatid infection in sheep. The results indicated that the infection rate of sheep with the resistant haplotype of hydatidosis was significantly lower (p<0.01) than without this resistant haplotype. It showed that the genic haplotype MvaIbc-SacIIab-Hin1Iab of Ovar-DRB1 exon 2 was the resistant haplotype of hydatidosis in Kazakh sheep.

A WIND TUNNEL EXPERIMENTAL STUDY OF ICING ON WIND TURBINE BLADE AIRFOIL

Yan LI,Kotaro TAGAWA,Fang FENG,Qingbin HE,Qiang LI 한국신재생에너지학회 2012 AFORE Vol.2012 No.-

JCAD deficiency attenuates activation of hepatic stellate cells and cholestatic fibrosis

Li Xie,Hui Chen,Li Zhang,Yue Ma,Yuan Zhou,Yong-Yu Yang,Chang Liu,Yu-Li Wang,Ya-Jun Yan,Jia Ding,Xiao Teng,Qiang Yang,Xiu-Ping Liu,Jian Wu 대한간학회 2024 Clinical and Molecular Hepatology(대한간학회지) Vol.30 No.2

Background/Aims: Cholestatic liver diseases including primary biliary cholangitis (PBC) are associated with active hepatic fibrogenesis, which ultimately progresses to cirrhosis. Activated hepatic stellate cells (HSCs) are the main fibrogenic effectors in response to cholangiocyte damage. JCAD regulates cell proliferation and malignant transformation in nonalcoholic steatoheaptitis-associated hepatocellular carcinoma (NASH-HCC). However, its participation in cholestatic fibrosis has not been explored yet. Methods: Serial sections of liver tissue of PBC patients were stained with immunofluorescence. Hepatic fibrosis was induced by bile duct ligation (BDL) in wild-type (WT), global JCAD knockout mice (JCAD-KO) and HSC-specific JCAD knockout mice (HSC-JCAD-KO), and evaluated by histopathology and biochemical tests. In situ-activated HSCs isolated from BDL mice were used to determine effects of JCAD on HSC activation. Results: In consistence with staining of liver sections from PBC patients, immunofluorescent staining revealed that JCAD expression was identified in smooth muscle α-actin (α-SMA)-positive fibroblast-like cells and was significantly up-regulated in WT mice with BDL. JCAD deficiency remarkably ameliorated BDL-induced hepatic injury and fibrosis, as documented by liver hydroxyproline content, when compared to WT mice with BDL. Histopathologically, collagen deposition was dramatically reduced in both JCAD-KO and HSC-JCAD-KO mice compared to WT mice, as visualized by Trichrome staining and semi-quantitative scores. Moreover, JCAD deprivation significantly attenuated in situ HSC activation and reduced expression of fibrotic genes after BDL. Conclusions: JCAD deficiency effectively suppressed hepatic fibrosis induced by BDL in mice, and the underlying mechanisms are largely through suppressed Hippo-YAP signaling activity in HSCs.

First Enzymatic Galactosylation of Acyclic Nucleoside Drugs by β-Galactosidase: Synthesis of Water-soluble β-D-Galactosidic Prodrugs

Li-Qiang Yan,Ning Liu,Min-Hua Zong 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.4

Acyclic nucleoside analogs constitute animportant group of antiviral agents. However, thesenucleoside drugs suffer from poor water solubility and loworal bioavailability in the clinic use. In the present work,the enzymatic synthesis of the water-soluble galactosidicprodrugs of acyclic nucleosides by using bovine liverβ-galactosidase was described. In the enzymatic transgalactosylationbetween acyclovir (ACV) and o-nitrophenylβ-galactopyranoside (oNPGal), the optimum enzyme dosage,buffer pH, temperature and molar ratio of ACV to oNPGalwere 0.225 U/mL, 7.0, 40°C and 2.5, respectively, underwhich the initial reaction rate and the yield reached0.40 mM/h and 29%, respectively. In addition, this enzymecould accept ganciclovir (GCV) and penciclovir (PCV) assubstrates, affording the corresponding 4’-β-galactosylatedderivatives with the yields of 26% and 71%, respectively.

Shear resistance of corrugated web steel beams with circular web openings: Test and machine learning-based prediction

Yan-Wen Li,Guo-Qiang Li,Lei Xiao,Michael C. H. Yam,JingZhou Zhang 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.1

This paper presents an investigation on the shear resistance of corrugated web steel beams (CWBs) with a circular web opening. A total of five specimens with different diameters of web openings were designed and tested with vertical load applied on the top flange at mid-span. The ultimate strengths, failure modes, and load versus middle displacement curves were obtained from the tests. Following the tests, numerical models of the CWBs were developed and validated against the test results. The influence of the web plate thickness, steel grade, opening diameter, and location on the shear strength of the CWBs was extensively investigated. An XGBoost machine learning model for shear resistance prediction was trained based on 256 CWB samples. The XGBoost model with optimal hyperparameters showed excellent accuracy and exceeded the accuracy of the available design equations. The effects of geometric parameters and material properties on the shear resistance were evaluated using the SHAP method.

Cucumber Growth and Nitrogen Uptake as Affected by Solution Temperature and NO3-:NH4+ Ratios during the Seedling

Qiu-yan Yan,Zeng-qiang Duan,Jun-hui Li,Xun Li,Jin-long Dong 한국원예학회 2013 원예과학기술지 Vol.31 No.4

The effect of solution temperature and nitrogen form on cucumber (Cucumis sativus L.) growth,photosynthesis and nitrogen metabolism was investigated in hydroponic culture. Cucumber plants were grown for 35 days in a greenhouse at three constant solution temperatures (15°C, 20°C, and 25°C) within a natural aerial temperature (15-30°C). Four nitrate:ammonium (NO3-:NH4+) ratios (10:0, 8:2, 5:5, and 2:8 mmol・L-1)at constant nitrogen (N) concentration of 10 mmol・L-1 were applied within each solution temperature treatment. Results showed an increasing solution temperature enhanced plant growth (height, dry weight, and leaf area)in most N treatments. Dry weight accumulation was greatest at the 10:0 NO3-:NH4+ ratio in the 15°C solution,the 5:5 ratio in the 20°C solution and the 8:2 ratio in the 25°C solution. Photosynthetic rate (Pn) response to solution temperature and NO3-:NH4+ ratio was similar to that of plant growth. Probably, the photosynthate shortage played a role in the reduced biomass formation. Increasing solution temperature enhanced the nitrate reductase (NR) activity, and further reduced shoots nitrate content. Our results indicate that the optimal ratio of nitrate to ammonium that promotes growth in hydroponic cucumber varies with solution temperature.

Cucumber Growth and Nitrogen Uptake as Affected by Solution Temperature and NO₃<SUP>-</SUP>

Qiu-yan Yan,Zeng-qiang Duan,Jun-hui Li,Xun Li,Jin-long Dong 한국원예학회 2013 원예과학기술지 Vol.31 No.4

The effect of solution temperature and nitrogen form on cucumber (Cucumis sativus L.) growth, photosynthesis and nitrogen metabolism was investigated in hydroponic culture. Cucumber plants were grown for 35 days in a greenhouse at three constant solution temperatures (15°C, 20°C, and 25°C) within a natural aerial temperature (15-30°C). Four nitrate:ammonium (NO₃<SUP>-</SUP>:NH₄<SUP>+</SUP>) ratios (10:0, 8:2, 5:5, and 2:8 mmol・L<SUP>-1</SUP>) at constant nitrogen (N) concentration of 10 mmol・L<SUP>-1</SUP> were applied within each solution temperature treatment. Results showed an increasing solution temperature enhanced plant growth (height, dry weight, and leaf area) in most N treatments. Dry weight accumulation was greatest at the 10:0 NO₃<SUP>-</SUP>:NH₄<SUP>+</SUP> ratio in the 15°C solution, the 5:5 ratio in the 20°C solution and the 8:2 ratio in the 25°C solution. Photosynthetic rate (Pn) response to solution temperature and NO₃<SUP>-</SUP>:NH₄<SUP>+</SUP> ratio was similar to that of plant growth. Probably, the photosynthate shortage played a role in the reduced biomass formation. Increasing solution temperature enhanced the nitrate reductase (NR) activity, and further reduced shoots nitrate content. Our results indicate that the optimal ratio of nitrate to ammonium that promotes growth in hydroponic cucumber varies with solution temperature.

Anti-metastasis Activity of Black Rice Anthocyanins Against Breast Cancer: Analyses Using an ErbB2 Positive Breast Cancer Cell Line and Tumoral Xenograft Model

Luo, Li-Ping,Han, Bin,Yu, Xiao-Ping,Chen, Xiang-Yan,Zhou, Jie,Chen, Wei,Zhu, Yan-Feng,Peng, Xiao-Li,Zou, Qiang,Li, Sui-Yan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: Increasing evidence from animal, epidemiological and clinical investigations suggest that dietary anthocyanins have potential to prevent chronic diseases, including cancers. It is also noteworthy that human epidermal growth factor receptor 2 (ErbB2) protein overexpression or ErbB2 gene amplification has been included as an indicator for metastasis and higher risk of recurrence for breast cancer. Materials and Methods: The present experiments investigated the anti-metastasis effects of black rice anthocyanins (BRACs) on ErbB2 positive breast cancer cells in vivo and in vitro. Results: Oral administration of BRACs (150 mg/kg/day) reduced transplanted tumor growth, inhibited pulmonary metastasis, and decreased lung tumor nodules in BALB/c nude mice bearing ErbB2 positive breast cancer cell MDA-MB-453 xenografts. The capacity for migration, adhesion, motility and invasion was also inhibited by BRACs in MDA-MB-453 cells in a concentration dependent manner, accompanied by decreased activity of a transfer promoting factor, urokinase-type plasminogen activator (u-PA). Conclusions: Together, our results indicated that BRACs possess anti-metastasis potential against ErbB2 positive human breast cancer cells in vivo and in vitro through inhibition of metastasis promoting molecules.