Acteoside reduces testosterone by inhibiting cAMP, p450scc, and StAR in rat Leydig cells

Shuqiang Liu,Junwen Zhang,Weixuan Li,Tianxiang Zhang,Defu Hu 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.1

The present study investigated the in vivo and in vitro effects of acteoside on testosterone production in Sprague-Dawley (SD) rats. The in vivo experiment revealed that acteoside reduced the testosterone level in serum significantly (P⁄0.05). The in vitro experiment also illustrated that acteoside significantly reduced testosterone production in SD rat Leydig cells in primary culture (P⁄0.05). Enzyme-linked immunosorbent assay results demonstrated that acteoside significantly reduced the cyclic adenosine 3′, 5′-monophosphate (cAMP) level (P⁄0.05), and Western blot analysis showed that acteoside significantly reduced cholesterol side-chain cleavage enzyme (p450scc) and steroidogenic acute regulatory (StAR) expression (P⁄ 0.05). Hoechst 33342 staining and Western blotting showed that acteoside did not induce apoptosis in Leydig cells. Together, these results suggest that the acteoside-induced reduction in testosterone production in rat may be at least partially due to down-regulation of cAMP, p450scc, and StAR, but not apoptosis.

Study on Stabilit of Surrounding Rock in Deep Tunnel and It’s Support Design in Donghai Mine

JIN Zhupeng,ZHANG junwen,LI yulin 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.7

In order to control the deformation of surrounding rock in deep mine and study the corresponding patterns, the determination of its mechanics parameters and property, numerical simulation and the design of support scheme on the surrounding rock in fifth mining area of Donghai mine are conductive to the study of effective control of deformation and failure. By analyzing the characteristics of deformation and failure of surrounding rock, its regularity and corresponding reasons are finally found—— high stress make rock soft and lead to the large deformation of surrounding rock, which inevitably contribute to the broken roof and heaved floor. In addition, based on the numerical simulation, we further analyze the stress distribution and deformation characteristics of surrounding rock and proposed the anchor- injection - net – cable, the double support scheme. We also came up with the whole sealing and coordinated supporter for the fracturing roadway of large deformation, especially in the intersection roadways, witch undoubtedly is beneficial to the research of deformation mechanism and control of surrounding rock in deep mine.

Preparation of Al2O3/AZ91D Mg Interpenetrating Composites Using Lost Foam Casting Combined with Layered Extrusion Forming

Wenming Jiang,Guangyu Li,Feng Guan,Junwen Zhu,Dongping Zhang,Zitian Fan 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.4

A novel method named lost foam casting (LFC) process combined with layered extrusion forming (LEF) technology wasproposed to prepare the Al2O3/AZ91D Mg interpenetrating composites, and the microstructural characteristics, thermalexpansion performance and wear resistance of the Al2O3/AZ91D interpenetrating composites were investigated in this work. The results indicated that a superior bonding between AZ91D magnesium alloy and Al2O3porous ceramic was achieved. The Al2O3/AZ91D interpenetrating composites exhibited obvious improvements in the thermal expansion performance andthe wear resistance compared to the AZ91D alloy. Therefore, the LFC process combined with the LEF technology providesa promising method for the preparation of the Al2O3/AZ91D interpenetrating composites.

Microstructure, Mechanical Properties and Fracture Behavior of Magnesium/Steel Bimetal Using Compound Casting Assisted with Hot-Dip Aluminizing

Wenming Jiang,Haixiao Jiang,Guangyu Li,Feng Guan,Junwen Zhu,Zitian Fan 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.8

In this work, microstructure, mechanical properties and fracture behavior of the magnesium/steel bimetal using compoundcasting assisted with hot-dip aluminizing were investigated, and the interface bonding mechanism of the magnesium/steelbimetal were also analyzed. The results indicate that the magnesium/steel bimetal obtained without hot-dip aluminizing hadlarger gaps through the whole interface without reaction layers between magnesium and steel, leading to a poor mechanicalbonding. After the steel substrate was hot-dip aluminized, an intermetallic layer along with an Al topcoat layer wereformed on the surface of the steel substrate, and the intermetallic layer was constituted by Fe2Al5,τ10-Al9Fe4Si3, FeAl3andτ6-Al4.5FeSi phases. In the case of the magnesium/steel bimetal obtained with hot-dip aluminizing, a compact and uniforminterface layer with an average thickness of about 17 μm that consisted of Fe2Al5,τ10-Al9Fe4Si3, FeAl3and Al12Mg17intermetalliccompounds was formed between the magnesium and the steel, obtaining a superior metallurgical bonding. The interfacelayer had much higher nano-hardnesses compared to the magnesium and steel matrixes, and its average nano-hardness wasup to 11.1 GPa, while there were respectively 1.1 and 4.2 GPa for the magnesium and steel matrixes. The shear strength ofthe magnesium/steel bimetal with hot-dip aluminizing reached to 23.3 MPa, which increased by 8.59 times than that of thecomposites without hot-dip aluminizing. The fracture of the magnesium/steel bimetal with hot-dip aluminizing representeda brittle fracture nature, initiating from the interface layer.

Experimental and Numerical Investigation of the Tensile Performance and Failure Process of a Modified Portland Cement

Jianhang Chen,Kangming Tao,Banquan Zeng,Lei Liu,Hongbao Zhao,Junwen Zhang,Danqi Li 한국콘크리트학회 2023 International Journal of Concrete Structures and M Vol.17 No.1

A better understanding of the tensile performance and tensile failure mechanism of cement paste is significant in preventing rock reinforcement failure. Therefore, this paper aims to reveal the tensile performance and failure mechanism of a modified Portland cement: Stratabinder HS cement. To achieve this objective, the split tensile test was conducted on specimens followed by simulating the failure mechanism numerically. The results indicated that the water–cement rate significantly influenced the tensile performance of the cement paste. When the water–cement rate increased from 0.35 to 0.42, the tensile strength declined from 1.9 MPa to 1.5 MPa. It was also observed that vertical tensile failure constantly occurred regardless of the water–cement rate. During the testing process, tensile cracks and shear cracks occurred. The increasing rate in the number of specimen cracks was dependent on the tensile stress state. Before the tensile stress reached the peak, the crack quantity increased slightly. After the peak, the crack quantity increased dramatically. During the vertical loading process, horizontal tensile stress occurred in the specimen. This horizontal tensile stress zone showed a diamond shape. The higher the tensile stress is, the larger the area of the horizontal tensile stress zone. When the tensile strength was reached, horizontal tensile stress mainly concentrated at the vertical centre of the specimen. This finally led to tensile failure of the specimen. This paper indicated that the water–cement rate was the key factor in evaluating the tensile strength of the Stratabinder HS cement.

Establishment of hybridization breeding of Ganoderma lucidum by protoplast monokaryogenesis method

Wu Jiaojiao,Fu Lizhong,Wu Xueqian,Xu Xiuhong,Li Haibo,Cheng Junwen,Wu Qingqi,Wei Hailong 한국버섯학회 2010 한국버섯학회 국제학술대회 Vol.6 No.-

Establishment of hybridization breeding of Ganoderma lucidum by protoplast monokaryogenesis method

Wu Jiaojiao,Fu Lizhong,Wu Xueqian,Xu Xiuhong,Li Haibo,Wu Qingqi,Wei Hailong,Cheng Junwen 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

Four Ganoderma lucidum strains, Chizhi 05, Jingda, Huizhou and Xinzhou, were screened out as hybrid parent in order to establish G. lucidum cross breeding system that based on protoplast monokaryogenesis method. Monokaryotic strains of each parental strains were obtained and mating type of each monokaryotic strains were determined. One to three monokaryotic strains that have different mating types were mated, and hybrids were identified by clamp connection observation and antagonist response. The results showed that the number of monokaryon came from Chizhi 05, Jingda, Huizhou and Xinzhou was 9, 14, 40 and 38, respectively. Only one mating type was obtained from Jingda, and two mating types were obtained from the other three strains, Chizhi 05, Huizhou and Xinzhou, respectively. Chi-square test showed that the ratio of two mating types of the three strains was 1:1. Fourteen monokaryotic strains of different mating types from 4 parental strains were select as a cross- breeding materia, and 17 hybrids were obtained, which were identified by clamp connection observation and antagonist response. This study proclaimed that the practicality of the hybridization breeding of G. lucidum by protoplast monokaryogenesis method.

W-대역 도파관 전압조정발진기를 위한 건 다이오드 마운트의 실험적설계

민재용,리준문,안병철,노진입,김동환,Min Jae-Yong,Li junwen,Ahn Bierng-Chearl,Roh Jin-Eep,Kim Dong-Hwan 한국전자파학회 2005 한국전자파학회논문지 Vol.16 No.1

본 논문에서는 W-대역 도파관 전압조정발진기에 적용할 수 있는 건 다이오드 마운트를 실험적으로 설계하였다. 건 다이오드 마운트에 의해서 건 다이오드의 낮은 임피던스는 도파관의 높은 임피던스에 정합된다. 건 다이오드 마운트에서 디스크 직경, 디스크 높이, 금속봉 직경에 따른 전압조정 발진기의 중심 주파수, 주파수 조정범위, 출력전력 등 특성 변화 경향을 시뮬레이션에 의해 확인한 후 실험적인 방법으로 최적의 건 다이오드 마운트를 설계하였다. 설계된 전압조정 발진기는 중심 주파수 93.9 GHz, $2{\%}$ 주파수 선형성 범위 600 MHz, 출력전력 16 dBm 등의 우수한 특성을 보였다. In this paper, the Gunn diode mount is experimentally designed for use in a W-band waveguide voltage controlled oscillator(VCO). The role of the Gunn diode mount is to match the low impedance of the Gunn diode to the high impedance of waveguide. Computer simulations of VCO characteristics such as center frequency, frequency tuning range, and output power are carried out for various values of disc diameter, disc height, post diameter, and utilized in the experimental optimization of the Gunn diode mount. The designed VCO shows excellent characteristics; 93.9 GHz center fiequency, 600 MHz frequency tuning range with $2{\%}$ linearity, 16 dBm output power.

High-temperature-mixing hydrothermal synthesis of ZnO nanocrystals with wide growth window

Jun Wen,Yonghong Hu,Kongjun Zhu,Yufang Li,Jizhong Song 한국물리학회 2014 Current Applied Physics Vol.14 No.3

High-quality and controllable growth of nanocrystals (NCs) have been attracting great attention. Here, a high-temperature-mixing hydrothermal (HTMH) method was designed to synthesize ZnO NCs with high crystallinity and narrow size distribution in a wide growth window. Compared with conventional hydrothermal (CH) growth, zinc source and alkali precursors were intentionally separated in temperaturerising stage and permitted to mix at the starting of heat preservation stage of HTMH growth. Highly crystalline ZnO NCs with uniform spherical morphology can be formed at alkali concentration and temperature windows as wide as 0.1e0.5 M and 160e200 C, respectively. However, the products via CH method have much larger changes in not only morphology but also size. These results demonstrated that the high-temperature-mixing reaction greatly facilitates nucleation but depresses grain growth. Considering the simplicity and reproducibility, such HTMH method could have wide potentials for the fabrication of various functional nanocrystals.