Apoptosis and necroptosis‑inducing effects of arctigenin on nasal septum carcinoma RPMI‑2650 cells in 2D and 3D culture

Yoon‑Jin Lee,Kwan‑Sik Park,Byoung Joon Baek,Kyong‑Ae Lee,Sang‑Han Lee 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Backgrounds Arctigenin derived from the seeds of Arctium lappa Linnaeus is known as an anticancer drug candidate by targeting various pathways involved in anticancer therapy. Methods Using 2D monolayer and 3D spheroid culture systems in nasal septum carcinoma RPMI-2650 cells, the effects of arctigenin and dexamethasone on cell viability, ROS levels, ATP level, mitochondrial function, apoptosis and necroptosis were examined. Results The combination treatment of both compounds induced strong cytotoxicity, accompanied by increases of sub-G0/ G1 peak, annexin V-PE-positive cells, and ROS levels, loss of mitochondrial membrane potential, and decrease of cellular ATP content. These changes were observed as simultaneous induction of DNA damage, apoptosis, and necroptosis. A series of changes by arctigenin and dexamethasone were efficiently restored by decreasing ROS levels or supplementing ATP. Treatment of 3D spheroids with arctigenin and dexamethasone decreased cell viability in the spheroids, but it was slightly resistant than cells under 2D conditions. In addition, this phenomenon was accompanied by an increase in mediators for both apoptosis and necroptosis. Conclusion Results of this study suggest that the apoptosis and necroptosis-inducing effects of arctigenin are associated with ATP depletion due to oxidative mitochondrial dysfunction.

중학교 복싱선수의 심리훈련이 심박수 및 운동발현에 미치는 영향

이윤관,이한우 창원대학교 체육과학연구소 2002 경남 체육연구 Vol.7 No.2

본 스포츠에서의 승패는 강한 체력과 고도의 기술 그리고 강인한 정신력의 집합체라고 할 수 있다. 체력이나 기술도 중요하지만 그보다 중요한 것은 심리적 요인인 정신력이라고 보고하였는데, 이는 정신력이 경쟁불안이나 스트레스 등과 같은 긴장상태가 유발되면 캐논의 정서 위급설에서와 같이 운동수행이 불가능해지는 사태가 발생하기 때문이라고 할 수 있다. 따라서 심리훈련이 복싱선수들에게 긴장과 불안감을 줄이며 집중력을 향상시키고, 또 복싱지도자들이 자신들의 경험에 의한 훈련지도에서 벗어나서 신체 훈련과 함께 심리훈련의 효과와 중요성을 인식하고 훈련 현장에 적용하여 침체되어 있는 복싱 경기력 향상의 계기가 되었으면 한다. 심리훈련의 결과는 심신의 건강에 긍정적인 효과를 나타내는 것으로 인식되고 있으며 특히, 스포츠의 장면에서는 경쟁불안이나 스트레스 해소에 비교적 그 효과가 크다고 보고되고 있는 반면, 신체적 변화를 위한 생리적 요인의 증강과 운동능력의 강화에 대해서는 이론이나 학설들이 각기 다른 양상을 보이고 있는 실정이므로 이에 대한 보완적인 연구가 계속적으로 필요하다. 본 연구는 J중학교 복싱선수 15명을 대상으로 심리훈련을 통하여 심박수, 혈압, 악력과 제자리멀리뛰기가 어떤 영향을 미치는가를 알아보기 위하여 12주 동안 심리훈련 전과 심리훈련 후 측정한 결과 다음과 같은 결론을 얻었다. 1. 심박수 심신훈련 전 평상시와 훈련 후의 비교에서 심박수에 있어서는 훈련 전, 후 의미 있는 차이가 나타났다(p<.05). 2. 혈압 수축기 혈압의 경우 의미 있는 차이가 나타났으나(p<.05) 이완기 혈압에 있어서는 의미 있는 차이가 나타나지 않았다. 3. 악력과 제자리멀리뛰기 심리훈련전과 훈련후의 비교에서는 악력과 제자리멀리뛰기에서 증가를 보였으나 유의한 차이는 나타나지 않았다.

고등학교 복싱선수의 지구성 훈련이 혈당, 혈청지질 및 지단백의 변화에 미치는 영향

이윤관,이영웅,권판근,이한우 창원대학교 체육과학연구소 2002 경남 체육연구 Vol.7 No.2

트레이닝에 따른 인체의 반응 및 적응성 변화 양상은 운동의 강도, 빈도, 형태 및 기간에 따라서 달라지며, 피검자의 단련수준에 따라서도 달라진다. 이러한 변화 요인 중에서 혈청지질(Serum Lipid) 지단백(Lipoprotein)의 수준 변화는 심장계 질환 및 관상동맥 질환의 예방과 치료에 있어서 의학계는 물론 스포츠 과학분야에 있어서도 매우 많은 관심을 갖고 있으며, 이에 대한 연구가 활발하게 진행되고 있다. 유산소적 운동은 혈청지질 및 지단백, 그리고 혈당에 미치는 영향이 크다는 견해이지만 실제 연습방법에 따른 이러한 변화의 특성에 관한 보고는 거의 없는 실정이다. 따라서 본 연구자는 질병 예방과 운동선수의 훈련 효과를 높이기 위해 복싱선수들을 대상으로 하여 연습방법을 달리한 지구성 훈련을 시켜 혈청지질 및 지단백 그리고 혈당의 변화를 비교, 분석하여 운동선수들의 연습방법에 도움을 줄 수 있는 자료를 제공하고자 한다. 집중적 연습과 분산적 연습에 따른 유산소 지구성 운동을 13주 4일간 실시한 후 혈당, 혈청지질 및 지단백의 변화에 미치는 영향을 알아보기 위해 복싱선수 15명을 대상으로 Total-Cholesterol, HDL-Cholesterol, Triglyceride, Total-protein 및 glucose를 측정하여 비교 분석한 결과 다음과 같은 결론을 얻었다. 1.Total-Cholesterol은 훈련전 보다 13주 4일간 훈련후 모든 집단에서 증가하였으며, 집단간에 유의한(p<.05) 차이를 나타내었다. 2.HDL-Cholesterol은 훈련전 보다 13주 4일간 훈련후 집중적 연습집단에서는 증가하였으며, 집단간 유의한(p<.05)차이를 나타내었다. 3.Triglyceride는 훈련전보다 13주 4일간 훈련후 모든 집단에서 감소하였으며, 집단간 매우 유의한(p<.001) 차이를 나타내었다. 4.Total-Protein은 훈련전 보다 13주 4일간 훈련후 모든 집단에서 증가하였으며, 집단간 매우 유의한(p<.001) 차이를 나타내었다. 5.Glucose는 훈련전보다 13주 4일간 훈련후 모든 집단에서 감소하였으며, 집단간 유의한(p<.05) 차이를 나타내었다.

표준 용매 벤젠의 Rayleigh 비의 온도 의존성

박일현,이종근,윤관한,조동환,민병길,방대석 金烏工科大學校 2000 論文集 Vol.21 No.-

The Rayleigh ratio of benzene is a very important physical constant with which the integrated scattered intensity of laser light scattering can be converted into the absolute value. In this study temperature dependence of Rayleigh ratio of this solvent has been investigated with two different methods in the temperature range of 25-75℃. It was found that in the direct measurement the non-linear behavior of its Rayleigh ratio were observed as increasing temperature even if the scattering volume correction was applied to the scattered intensity of benzene. However the result of indirect measurement based upon the invariance of molecular weight of standard polystyrene polymer for any polymer solution temperature showed us that its temperature dependence up to 75℃ could be expressed with the Benoit's linear equation rather than the non-linear one. It seems that the scattered intensity in direct method has more complicate contributions at the elevated temperature. Further research works are necessary in order to solve such discrepancy clearly.

의료관계 해부 감정예의 분석적 연구(I) : 1990년 국립과학수사연구소 감정예

姜信夢,李垣兌,李漢榮,尹順雄,全基悳,金相鉉,徐在冠,尹重鎭 大韓法醫學會 1991 대한법의학회지 Vol.15 No.2

A study of 118 autopsy cases associated with medical care in 1990 was present according to the medical department, the mode of treatment and the cause of death. The Pediatrics showed the highest rate (25.4%), followed by the Obstetrics & Gynecology (22.9%), and Internal medicine (20.2%). According to the mode of treatment, medical accidents during operation and anesthesia were 29 cases (24.6%) with highest rate in Obstetrics & Gynecology (13 cases), 38 cases (32.2%) were during medication and/or injection with highest rate in Internal Medicine (18 cases), and 51 cases (43.2%) were during general medical practice with highest rate in pediatrics (19 cases). The most prevalent cause of death was the disease of respiratory system (34.9%, 30 cases) and the diseases of cardiovascular and urogenital system accounted for 31.4% and 11.6% respectively. The main cause of death in Internal medicine was the disease of cardiovascular system (11/24 cases), the respiratory system in pediatrics (15/30 cases) and the disease of urogenital system in Obstetrics & Gynecology (9/27 cases. ) Asphyxial deaths were 7 cases and the death due to adverse drug reaction accounted for 10 cases.

사람폐암세포주 (PC-14)에서 Cyclosporin A에 의한 Adriamycin 내성의 극복

김영환,홍원선,송재관,강윤구,이진오,강태웅,김건열,한용철 대한내과학회 1990 대한내과학회지 Vol.38 No.3

Cyclosporin A and verapamil were tested using MTT assay to evalute the modification effect on the resistance to adriamycin in a human lung cancer cell line(PC-14) and its resistant subline(PC-14/A). PC-14/A was derived by the continuous exposure of PC-14 to incremental concentrations of adriamycin. PC-14/A was 2.5 times more resistant to adriamycin in terms of ICso than PC-14. Cyclosporin A alone, at a concentration of 2.5㎍/㎖, inhibited the growth of PC-14 to 68.3%. 2.5㎍/ ㎖ and 5.0㎍/㎖ of cyclosporin A showed an increase in the cytotoxicity of adriamycin (p<0.01) with 5.0㎍/㎖ being greater than 2.5㎍/㎖(p<0.01). Excluding the direct cytotoxic effect, however, cyclosporin A did not increase in the sensitivity of PC-14 to adriamycin but only showed an additional cytotoxic effect with adriamycin. Verapamil (up to 6.0㎍/㎖) did not inhibit the growth of PC-14. 3.0㎍/㎖ of verapamil did not increase the cytotoxic effect of adriamycin. The combination of cyclosporin A and verapamil with adriamycin enhanced the cytotoxicity of adriamycin, but the result was similar to that of cyclosporin A with adriamycin. 5.0㎍/㎖ of cyclosporin A modified the adriamycin resistance of PC-14/A(SR, 3.2). However, 3.0㎍/㎖ of verapamil did not significantly reverse the adriamycin resistance of PC-14/A. The modified effect of the combination of 5.0㎍/㎖ of cyclosporin A and 3.0㎍/㎖ of verapamil was similar to that of 5.0㎍/㎖ of cyclosporin A alone in PC-14/A. These results demonstrate that cyclosporin A has an additional cytotoxic effect with adriamycin in PC-14 and PC-14/A and has overcome the acquired resistance to adriamycin in PC-14/A. They also suggest that cyclospoin A may have the therapeutic potential in the treatment of human lung cancer.

Comparison in Disease Development and Gas Exchange Rate of Pinus densiflora Seedlings Artificially Inoculated with Bursaphelenchus xylophilus and B. mucronatus

( Kwan Soo Woo ),( Jun Hyuck Yoon ),( Su Young Woo ),( Seong Han Lee ),( Sang Urk Han ),( Hye Rim Han ),( Saeng Geul Baek ),( Chang Soo Kim ) 한국산림과학회 2010 Forest Science And Technology Vol.6 No.2

Four-year-old seedlings of Pinus densiflora were inoculated with a suspension of B. xylophilus, or B. mucronatus adjusted to 3,000 nematodes per 50 μL sterilized distilled water in a greenhouse on July 21, 2008 to evaluate initial symptom development and the changes of gas exchange rate. B. xylophilus and B. mucronatus were distinguished by four restriction enzymes except Rsa I, and the result of ITSRFLP of B. xylophilus used in this experiment was completely matched to that of pine wood nematode reported previously. Needle dehydration and subsequent yellowing were observed in all seedlings inoculated with B. xylophilus isolate while the appearance of the seedlings was normal in all seedlings inoculated with B. mucronatus and control. Needle dehydration was observed in most seedlings inoculated with B. xylophilus between 2 and 3 weeks after inoculation. In seedlings inoculated with B. xylophilus, continuous decrease in photosynthetic rate was observed after 6 days of inoculation. Photosynthetic rate decreased more markedly after 12 days of inoculation when external symptoms appeared in most seedlings, and ceased almost completely 19 days after inoculation. Photosynthetic decline in seedlings inoculated with B. mucronatus was only observed at 19 days after inoculation but recovered above control level after 25 days of inoculation. We found that disease development and the changes of gas exchange rate in the seedlings of Pinus densiflora inoculated with B. xylophilus were not observed on those inoculated with B. mucronatus.

Effect of Heat-treatment on Stretch of Poly(trimethylene terephthalate) Woven Fabric

Yoon, Kwan-Han,Jeong, Young-Jin,Min, Byung-Ghyl The Korean Fiber Society 2003 Fibers and polymers Vol.4 No.2

The properties of a woven fabric made of poly(trimethylene terephthalate) (PTT) were investigated. The PTT fab-ric of draw textured yarn (DTY) showed excellent stretch as good as a fabric containing spandex. However, the unique stretch of the PTT fabric reduced dramatically by simple heat-treatment even at as low as $80^{\circ}C$. To understand the phenomenon, the crimp rigidity of the DTY was observed by SEM. It was found that the drastic reduction of stretch was caused by irreversible uncrimping of PTT DTY after heat-treatment. Conclusively, it is of importance to optimize the texturing conditions for PTT DTY to make the crimp more stable.

Damping Properties and Transmlission Loss of Polyurethane. II. PU Layer and Copolymer Effect

Yoon, kwan-Han,Kim, Ji-Gon,Bang, Dae-Suk The Korean Fiber Society 2003 Fibers and polymers Vol.4 No.2

Polyurethane (PU) layer and copolymer consisted of the different molecular weights (1000 and 2000 g/mol) of poly(propylene glycol) (PPG) were prepared. The damping and mechanical properties of these materials were compared with PU 1000 made by PPG having the molecular weight of 1000 g/mol. The optimum composition of PU2000 used for PU layer and copolymer was diphenylmethane diioscynate (MDI)/propylene glycol (PPG)/butanediol (BD) (1/0.3/0.7) based on the damping and mechanical properties. The damping peak of PU copolymer was higher than those of PU layer and PUI 1000 in low temperature range (-30- $10^{\circ}C$). For application in noise reduction, the transmission loss of the mechanical vibration through solid structure was measured. PU layer and copolymer were used as a damping layer. The transmission loss of PU copolymer was more effective than those of PU layer and PU 1000 in the experimental frequency range.

Properties of poly(ethylene terephthalate) containing epoxy-functionalized polyhedral oligomeric silsesquioxane

Yoon, Kwan Han,Polk, Malcolm B,Park, Jae Hong,Min, Byung G,Schiraldi, David A John Wiley Sons, Ltd. 2005 Polymer international Vol.54 No.1

<P>Poly(ethylene terephthalate) (PET) containing epoxy-functionalized polyhedral oligomeric silsesquioxane (POSS) was prepared by melt-mixing and in situ polymerization methods. The melt-mixed composite showed phase separation while the in situ polymerized composite did not, based on SEM characterization. During melt mixing, the reaction between the epoxy groups of POSS and hydroxyl groups of PET occurred, based on DSC results. DSC results on the in situ polymerization product showed formation of a lower-melting component compared with PET. The tensile strength and modulus of the melt-mixed composite fiber decreased compared with those properties of PET, whereas those of the in situ polymerized composite showed slightly higher values than PET despite the relatively small amounts (1 wt%) of POSS used. Dynamic mechanical analysis results showed an increase in storage modulus for the in situ polymerized composite of POSS and PET compared with PET over the temperature range of 40 °C to 140 °C. Copyright © 2004 Society of Chemical Industry</P>