Cardiac Cell Differentiation of Mouse Embryonic Stem Cells in the Co-culture with Fetal Heart

Koung-min Park,Eun-Kyoung Kim,Hae-Lim Kim,Ok-Seong Moon,Young-Suk Won,Hyoung-Chin Kim,Byeongwoo Ahn,Seung-Ho Han,Ki-Hoan Nam 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.3

To investigate the effect of fetal cardiac cells on the differentiation of embryonic stem cells (ESCs) into cardiomyocytes, Mouse ESCs were co-cultured with heart cells prepared from fetus aged 11.5, 12.5, 13.5 or 14.5 days postcoitum (dpc). The fetal mouse heart was from ICR and 129 strains from which KH2 ES cell line was derived. In the present study, KH2 cells expressing EGFP directed by α-myosin heavy chain gene promoter were co-cultured with fetal heart isolated at different fetal stages. The cardiomyocytes derived from ESC in the co-culture could be identified by their EGFP expression. The EGFP expressing cells which were confirmed to be cardiomyocytes were measured as an indicator of cardiac cell differentiation of ESC. The fetal cardiac cells from 12.5 dpc were more effective in cardiac cell differentiation of ESC than the cells from fetus aged 11.5, 13.5 or 14.5 dpc. Fetal heart from 129 strain which is immunologically compatible with KH2 cells showed more strong cardiac cell differentiation induction activity compared with that from ICR mice. As the results, we demonstrated that fetal hearts at different developmental stages have different cardiac cell differentiation induction activity from ESC implying that fetal heart expresses cardiac cell differentiation induction factor(s).

Functional Magnetic Resonance Imaging and Diffusion Tensor Imaging for Language Mapping in Brain Tumor Surgery: Validation With Direct Cortical Stimulation and Cortico–Cortical Evoked Potential

Kang Koung Mi,Kim Kyung Min,Kim In Seong,Kim Joo Hyun,Kang Ho,Ji So Young,Dho Yun-Sik,Oh Hyongmin,Park Hee-Pyoung,Seo Han Gil,Kim Sung-Min,Choi Seung Hong,Park Chul-Kee 대한영상의학회 2023 Korean Journal of Radiology Vol.24 No.6

Objective: Functional magnetic resonance imaging (fMRI) and diffusion tensor imaging-derived tractography (DTI-t) contribute to the localization of language areas, but their accuracy remains controversial. This study aimed to investigate the diagnostic performance of preoperative fMRI and DTI-t obtained with a simultaneous multi-slice technique using intraoperative direct cortical stimulation (DCS) or corticocortical evoked potential (CCEP) as reference standards. Materials and Methods: This prospective study included 26 patients (23–74 years; male:female, 13:13) with tumors in the vicinity of Broca’s area who underwent preoperative fMRI and DTI-t. A site-by-site comparison between preoperative (fMRI and DTI-t) and intraoperative language mapping (DCS or CCEP) was performed for 226 cortical sites to calculate the sensitivity and specificity of fMRI and DTI-t for mapping Broca’s areas. For sites with positive signals on fMRI or DTI-t, the true-positive rate (TPR) was calculated based on the concordance and discordance between fMRI and DTI-t. Results: Among 226 cortical sites, DCS was performed in 100 sites and CCEP was performed in 166 sites. The specificities of fMRI and DTI-t ranged from 72.4% (63/87) to 96.8% (122/126), respectively. The sensitivities of fMRI (except for verb generation) and DTI-t were 69.2% (9/13) to 92.3% (12/13) with DCS as the reference standard, and 40.0% (16/40) or lower with CCEP as the reference standard. For sites with preoperative fMRI or DTI-t positivity (n = 82), the TPR was high when fMRI and DTI-t were concordant (81.2% and 100% using DCS and CCEP, respectively, as the reference standards) and low when fMRI and DTI-t were discordant (≤ 24.2%). Conclusion: fMRI and DTI-t are sensitive and specific for mapping Broca’s area compared with DCS and specific but insensitive compared with CCEP. A site with a positive signal on both fMRI and DTI-t represents a high probability of being an essential language area.

Costs and clinical outcomes of patients with diffuse large B-cell lymphoma in first remission: role of PET/CT surveillance

( Koung Jin Suh ),( Ki Hwan Kim ),( Ryul Kim ),( Ja Min Byun ),( Miso Kim ),( Jin Hyun Park ),( Bhumsuk Keam ),( Tae Min Kim ),( Jin-soo Kim ),( In Sil Choi ),( Dae Seog Heo ) 대한내과학회 2019 The Korean Journal of Internal Medicine Vol.34 No.4

Background/Aims: The role of [18F]-f luorodeoxyglucose positron emission tomography-computed tomography (PET/CT) in patients with diffuse large B-cell lymphoma (DLBCL) in first remission is unclear. Methods: Medical costs within the first 3 years of treatment completion and clinical outcomes of 118 patients with DLBCL in first remission with and without surveillance PET/CT (PET/CT [+] group [n = 76] and PET/CT [-] group [n = 42], respectively) were retrospectively analyzed. Results: In a propensity matched cohort with adjustment for International Prognostic Index risk and relapse, the PET/CT (+) group was shown to have similar medical costs as the PET/CT (-) group. Relapse-free survival (RFS) and overall survival (OS) were comparable between the two groups (median RFS not reached [NR] for both groups, p = 0.133; median OS NR, p = 0.542). Among 76 patients with surveillance PET/CT, 31 (40.8%) had findings suggestive of recurrence and 16 of these (51.6%) were later confirmed to have recurrent disease. Fifteen patients (48.4%) were confirmed to not have recurrence after follow-up CT or PET/CT evaluation (n = 10) and biopsy (n = 4). None of the patients with negative PET/CT findings had disease recurrence. Sensitivity, specificity, positive predictive value, and negative predictive value of PET/CT for detection of recurrence were 1, 0.75, 0.52, and 1, respectively. Conclusions: Surveillance PET/CT resulted in similar clinical outcomes and medical costs compared to no surveillance PET/CT. Approximately half of patients with PET/CT findings of recurrence had no recurrence after follow-up imaging and biopsy, which would not have been carried out if PET/CT had not been performed in the first place.

Antidepressant-like effects of the traditional Chinese medicine kami-shoyo-san in rats

PARK, SUNG-WOO,KIM, YOUNG-KOUNG,LEE, JUNG-GOO,KIM, SUN-HEE,KIM, JAE-MIN,YOON, JIN-SANG,PARK, YONG-KI,LEE, YONG-KYU,KIM, YOUNG-HOON Blackwell Publishing Asia 2007 PSYCHIATRY AND CLINICAL NEUROSCIENCES Vol.61 No.4

<P>Abstract </P><P>Kami-shoyo-san (KSS), a traditional Chinese medicine, has been used to treat patients with neuropsychiatric disorders. The aim of the present paper was to investigate whether KSS has antidepressant-like effects, and to assess its mechanism of action, using male Sprague–Dawley rats given 10-fold (KSS 10X) or 20-fold (KSS 20X) the typical human daily dosage. Immobility time was measured by the forced swimming test, and hippocampal neurogenesis was quantified under immobilization stress. Rats given KSS 20X, but not those given KSS 10X, had a significantly lower immobility time and improved neurogenesis in the hippocampus. These results suggest that KSS possesses an antidepressant-like effect at a behavioral and molecular level.</P>

이명환자에서 고실내 주입된 리도카인이 이음향방사에 미치는 영향

장민호,고의경,박중환,이석훈,전경명 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.9

부산지역에서 유행한 계절인플루엔자바이러스의 유전자 특성 및 계통분석(’06~’08 절기)

Yon-Koung Park(박연경),Nam-Ho Kim(김남호),Seung-Hwa Choi(최성화),Mi-Oak Lee(이미옥),Sang-Kee Min(민상기),Seong-Joon Kim(김성준),Kyung-Soon Cho(조경순),Young-Nan Na(나영란) 한국생명과학회 2010 생명과학회지 Vol.20 No.3

2006년 10월부터 2008년 6월까지 총 인플루엔자 의사 환자 1,822건의 인후도찰물 및 비인후도찰물에서 277건의 인플루엔자바이러스를 분리했다. 절기별로는 2006~2007 절기의 1,154검체 중 52건(4.5%), 2007~2008절기의 668검체 중 210건(31.4%)에서 인플루엔자바이러스를 분리하였다. 인플루엔자바이러스 A/H1N1의 HA 유전자의 경우, 2008~2009 절기의 백신주인 A/Brisbane/59/2007과는 96.7%~97.7%, A/Solomon Islands/3/2006 96.5%~97.3%, A/New Caledonia/20/99와는 95.6%~96.6%의 유사성을 나타냈으며, NA 유전자의 경우, A/Brisbane/59/2007과는 97.8%~98.5%, A/Solomon Islands/3/2006과는 96.7%~97.6%, A/New Caledonia/20/99와는 96.8%~97.7%의 유사성을 보여 2008~2009절기의 백신주인 A/Brisbane/59/07과 가장 유사성이 컸다. 인플루엔자바이러스 A/H3N2의 분리주 중 1주를 제외한 모든 분리주가 HA 유전자에서 2008~2009 절기 백신주인 A/Brisbane/10/2007과는 98.4%~99.7%의 유사성을 보였고, A/Wisconsin/67/2005와는 96.5%~97.5%의 유사성을 보였으며, NA 유전자에서는 A/Brisbane/10/2007과는 98.9%~99.4%, A/Wisconsin/67/2005와는 98.0%~98.6%, A/California/7/2004와는 98.3%~98.9%의 유사성을 보였다. 인플루엔자바이러스 B의 HA 유전자의 경우는 2주를 제외하고는 2008~2009 절기의 백신주인 B/Florida/4/2006과는 96.5%~99.7%의 유사성을 보였으며, B/Malaysia/2506/2004와는 86.7%~87.7%의 유사성을 보여 B/Florida/4/2006과의 유사성이 크게 나타났다. NA 유전자의 경우는 reassortant분리주가 96.7%와 97.3%의 유사성을 나타내는 것을 제외하고는 B/Florida/4/2006에 98.9%~100%의 유사성을 나타냈으며, 분리주 유행시기의 백신주인 B/Malaysia/2506/2004와는 94.5%~96.7%의 유사성을 나타내어 2008~2009 절기의 백신주와 더 큰 유사성을 보였다. HA 유전자에서는 conserverd receptor binding site는 아미노산의 치환 없이 모든 분리주에서 잘 보존되어 있었으며, N-linked glycosylation site도 인플루엔자바이러스 A/H1 1주, A/H3 1주를 제외하고는 모두 같은 수의 N-linked glycosylation sites를 가졌으며, 인플루엔자바이러스 B의 경우는 2008~2009 절기의 백신주보다 1개가 많은 4개의 N-linked glycosylation sites를 가지고 있었다. Antigenic sites의 경우는 인플루엔자바이러스 A/H1의 Sb의 3개의 아미노산에서 백신주들과 다른 아미노산을 가지고 있으며, A/H3에서는 A, B, E 부위에서는 아미노산의 변화가 나타났고, C, D 부위에서는 변화가 없었다. 인플루엔자바이러스 B의 4개의 분리주에서는 150 loop와 160 loop에서 B/Florida/4/2006과 비교하여 1개의 아미노산에서 치환이 나타났으며, 190 helix에서 모든 분리주가 B/Florida/4/2006과 비교하여 1개의 아미노산에서 치환이 나타났다. To monitor newly emerged influenza virus variants and to investigate the prevalence pattern, our laboratory performed isolation of the viruses from surveillance sentinel hospitals. In the present study, we analysed influenza A/H1N1, A/H3N2, B viruses isolated in Busan during the 2006/07 and 2007/08 seasons by sequence analysis of the hemagglutinin (HA1 subunit) and neuraminidase (NA) genes. The isolates studied here were selected by the stratified random sample method from a total of 277 isolates, in which 15 were A/H1N1, 16 were A/H3N2 and 29 were B. Based on the phylogenetic tree, the HA1 gene showed that A/H1N1 isolates had a 96.7% to 97.7% homology with the A/Brisbane/59/2007, A/H3N2 isolates had a 98.4% to 99.7% homology with the A/Brisbane/10/2007, and B isolates had a 96.5% to 99.7% homology with the B/Florida/4/2006(Yamagata lineage), which are all the vaccine strains for the Northern Hemisphere in 2008~2009 season. In the case of the NA gene, A/H1N1 isolates had 97.8% to 98.5% homologies, A/H3N2 isolates had 98.9% to 99.4% homologies, and B isolates had 98.9% to 100% homologies with each vaccine strain in the 2008~2009 season, respectively. Characterization of the hemagglutinin gene revealed that amino acids at the receptor-binding site and N-linked glycosylation site were highly conserved. These results provide useful information for the control of influenza viruses in Busan and for a better understanding of vaccine strain selection.

Prognostic effects of abnormal DNA damage response protein expression in breast cancer

Suh, Koung Jin,Ryu, Han Suk,Lee, Kyung-Hun,Kim, Hyojin,Min, Ahrum,Kim, Tae-Yong,Yang, Yaewon,Lee, Han-Byoel,Moon, Hyeong-Gon,Han, Sae-Won,Oh, Do-Youn,Han, Wonshik,Park, In Ae,Noh, Dong-Young,Im, Seock Springer-Verlag 2019 Breast cancer research and treatment Vol.175 No.1

Fibrous Dysplasia of Facial Bones Clinical Review of 6 Cases

李英敏,朴敬浩,李秉春,朱良子 醫藥係社 1963 綜合 醫學 Vol.8 No.10

Acute tubular necrosis as a part of vancomycin induced drug rash with eosinophilia and syste­mic symptoms syndrome with coincident post­infectious glomerulonephritis

Kim, Kyung Min,Sung, Kyoung,Yang, Hea Koung,Kim, Seong Heon,Kim, Hye Young,Ban, Gil Ho,Park, Su Eun,Lee, Hyoung Doo,Kim, Su Young The Korean Pediatric Society 2016 Clinical and Experimental Pediatrics (CEP) Vol.59 No.3

Drug rash with eosinophilia and systemic symptoms (DRESS) syndrome is a rare and potentially fatal condition characterized by skin rash, fever, eosinophilia, and multiorgan involvement. Various drugs may be associated with this syndrome including carbamazepine, allopurinol, and sulfasalazine. Renal involvement in DRESS syndrome most commonly presents as acute kidney injury due to interstitial nephritis. An 11-year-old boy was referred to the Children's Hospital of Pusan National University because of persistent fever, rash, abdominal distension, generalized edema, lymphadenopathy, and eosinophilia. He previously received vancomycin and ceftriaxone for 10 days at another hospital. He developed acute kidney injury with nephrotic range proteinuria and hypocomplementemia. A subsequent renal biopsy indicated the presence of acute tubular necrosis (ATN) and late exudative phase of postinfectious glomerulonephritis (PIGN). Systemic symptoms and renal function improved with corticosteroid therapy after the discontinuation of vancomycin. Here, we describe a biopsy-proven case of severe ATN that manifested as a part of vancomycin-induced DRESS syndrome with coincident PIGN. It is important for clinicians to be aware of this syndrome due to its severity and potentially fatal nature.

Transforming Growth Factor β Receptor Type I Inhibitor, Galunisertib, Has No Beneficial Effects on Aneurysmal Pathological Changes in Marfan Mice

( Jeong-ho Park ),( Min-seob Kim ),( Seokran Ham ),( Eon Sub Park ),( Koung Li Kim ),( Wonhee Suh ) 한국응용약물학회 2020 Biomolecules & Therapeutics(구 응용약물학회지) Vol.28 No.1

Marfan syndrome (MFS), a connective tissue disorder caused by mutations in the fibrillin-1 (Fbn1) gene, has vascular manifestations including aortic aneurysm, dissection, and rupture. Its vascular pathogenesis is assumed to be attributed to increased transforming growth factor β (TGFβ) signaling and blockade of excessive TGFβ signaling has been thought to prevent dissection and aneurysm formation. Here, we investigated whether galunisertib, a potent small-molecule inhibitor of TGFβ receptor I (TβRI), attenuates aneurysmal disease in a murine model of MFS (Fbn1^C1039G/+) and compared the impact of galuninsertib on the MFSrelated vascular pathogenesis with that of losartan, a prophylactic agent routinely used for patients with MFS. Fbn1^C1039G/+ mice were administered galunisertib or losartan for 8 weeks, and their ascending aortas were assessed for histopathological changes and phosphorylation of Smad2 and extracellular signal-regulated kinase 1/2 (Erk1/2). Mice treated with galunisertib or losartan barely exhibited phosphorylated Smad2, suggesting that both drugs effectively blocked overactivated canonical TGFβ signaling in Fbn1^C1039G/+ mice. However, galunisertib treatment did not attenuate disrupted medial wall architecture and only partially decreased Erk1/2 phosphorylation, whereas losartan significantly inhibited MFS-associated aortopathy and markedly decreased Erk1/2 phosphorylation in Fbn1C1039G/+ mice. These data unexpectedly revealed that galunisertib, a TβRI inhibitor, showed no benefits in aneurysmal disease in MFS mice although it completely blocked Smad2 phosphorylation. The significant losartaninduced inhibition of both aortic vascular pathogenesis and Smad2 phosphorylation implied that canonical TGFβ signaling might not prominently drive aneurysmal diseases in MFS mice.