주차원단위 산정 모형 개발에 관한 연구 -광주광역시 공동 주택 아파트를 대상으로-

권성대,고동봉,박제진,하태준,Kwon, Sung-Dae,Ko, Dong-Bong,Park, Je-Jin,Ha, Tae-Jun 대한토목학회 2014 대한토목학회논문집 Vol.34 No.2

도시의 급격한 팽창과 함께 주택부족 현상이 나타나게 되자, 정부는 주택부족 문제 해결을 위해 대규모 택지개발을 통하여 주택보급을 확대시켰다. 이러한 현상으로, 공동주택은 우리나라 전체 주택의 83% 수준을 유지하고 있고, 그 중 아파트가 차지하는 비중은 50%로 꾸준한 증가 추세를 보이고 있다. 이로 인해 아파트의 경우 입주민들의 승용차 보유 증가에 따른 아파트 단지 내 주차공간 부족문제 등 제반 주차 관련 문제가 발생하고 있다. 특히, 주차계획대수 수립 시 교통영향평가의 주차수요예측 중 전용면적을 고려한 주차원단위 산정 방법은 기존 계획보다 세대수는 증가하여도 전용면적이 작아지면 계획주차대수는 감소하는 것으로 나타나, 보다 현실적인 주차원단위 산정이 필요한 실정이다. 이에 본 연구는 공공주택 아파트를 대상으로 현실에 적합한 주차원단위를 산정하고자 한다. 현장조사 및 설문조사를 실시하고, 구득자료에 대한 분석을 수행함으로써, 기존 교통영향평가의 주차원단위 산정 문제점을 도출하였다. 또한, 주차수요예측에 영향을 미치는 요인 선정을 통해 주차원단위 산정모형을 개발하였다. 마지막으로 실제 조사된 아파트 주차원단위 자료를 통해 기존 교통영향평가의 주차원단위 산정과 본 연구에서 제시한 주차원단위 산정모형을 비교 분석하였다. 향후 본 연구에서 개발된 주차원단위 산정모형은 주차장법 기준 정립은 물론 보다 현실적인 주차수요예측 수행에 적극 활용될 수 있을 것으로 판단된다. The rapid expansion of cities led to the shortage of housing in urban areas. The government compensated for this shortage through large scale residential developments that increased the housing supply. The supply of condominium apartments remains above 83% of the entire housing supply, and the proportion of apartments are at a steady increase, at about 50%. Due to the increase, illegally parked cars resulting from the shortage of parking spaces within the apartment complex have become increasingly problematic as they block the transit of emergency vehicles, and heighten the tension among neighboring residents in obtaining a parking space. Especially, the future residents are considered to plan the parking based on the estimated demand for parking. However, the parking unit method utilized to estimate the parking demand accounts for the exclusive use of space, which is believed to be far from the parking demands in reality. The reason for this discrepancy is that, as the number of households decrease, and area of exclusive space is expanded, the planned parking increases. On the other hand, when the number of households increase, and the area of exclusive space is reduced, the planned parking decreases, thus methods to recalculate the parking units based on estimated parking demand is an urgent concern. To estimate the parking units based on condominium apartments, this study first examined the existing research literature, and appointed the field of investigation to collect the necessary data. In addition, field study data and surveys collected and analyzed, in order to identify the problems underlying parking units, and problems regarding the current traffic impact assessment parking unit calculation method were deduced. Through identifying the influential factors on parking demand estimates, and performing a factorial analysis based on the collected data, the variables were selected in relation to the parking demand estimates, to develop the parking unit estimate model. Finally, through comparing and verifying the existing traffic impact assessment parking unit estimate against the newly developed model using collected data, a far more realistic parking unite estimate was suggested, reflecting the characteristics of the residents. The parking unit estimate model developed in this study is anticipated to serve as the guidelines for future parking lot legislature, as wel as the basis to provide a more realistic estimate of parking demands based on the resident characteristics of an apartment complex.

12M의 고해상도 360° 카메라를 사용한 주차장의 14면 주차 상태 판단 프로그램

이영지(Young-Ji Lee),이희열(Hee-Yeol Lee),고태영(Tae-Young Ko),곽동훈(Dong-Hoon Kwak),김재형(Jae-Hyung Kim),김주호(Joo-Ho Kim),오승진,이태윤(Tae-Yoon Lee),박상민(Sang-Min Park),이승호(Seung-Ho Lee) 대한전자공학회 2019 대한전자공학회 학술대회 Vol.2019 No.6

In this paper, we propose a program to identify 14 parking status in a parking lot using a high resolution 360° camera of 12M. The proposed program consists of three steps: Match with plane image, parking area detection algorithm, and discrimination of parking using learning method. Tests on a model car to evaluate the program to identify 14 parking status in a parking lot using a high-resolution 360° camera of 12M showed 100% accuracy for both parking and double parking. Therefore, the effectiveness of a program to identify 14 parking status in a parking lot using a high-resolution 360° camera of 12M proposed in this paper has been proved.

Protein kinase A-induced phosphorylation at the Thr154 affects stability of DJ-1

Ko, Yeon Uk,Kim, Su-Jeong,Lee, Juhyung,Song, Min-Young,Park, Kang-Sik,Park, Jun Bae,Cho, Hyun-Soo,Oh, Young J. Elsevier 2019 Parkinsonism & related disorders Vol.66 No.-

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>Most cases of Parkinson's disease (PD) are sporadic, but genetic variations have been discovered in PD patients. PARK7/DJ-1 is a known cause of early-onset autosomal-recessive PD and is implicated in neuroprotection against oxidative stress. Although several post-translational modifications of DJ-1 have been proposed, phospho-modification of DJ-1 and its functional consequences have been less studied.</P> <P><B>Methods</B></P> <P>Putative phosphorylation sites of DJ-1 were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS analysis). Subsequently, phosphorylation site of DJ-1 was confirmed by <I>in vitro</I> kinase assay and cell-based pull-down assay. Impaired dimer formation of phospho-null mutant was measured using DSS crosslinking assay and immunoprecipitation assay. To evaluate physiological consequences of this event, protein stability of DJ-1 WT and DJ-1 phospho-null mutant were compared using cycloheximide chase assay and ubiquitination assay.</P> <P><B>Results</B></P> <P>Here, we showed that DJ-1 directly bound to the catalytic subunit of protein kinase A (PKAcα). We found that PKAcα is responsible for phosphorylation of DJ-1 at the T154 residue. Interestingly, dimerization of DJ-1 was not detected in a DJ-1 T154A mutant. Furthermore, stability of the DJ-1 T154A mutant was dramatically reduced compared with that of wild-type DJ-1. We found that DJ-1 T154A was prone to degradation by the ubiquitin proteasome system (UPS).</P> <P><B>Conclusion</B></P> <P>We identified a novel phosphorylation site of DJ-1. Furthermore, we determined protein kinase A that is responsible for this posttranslational modification. Finally, we demonstrated physiological consequences of this event focusing on dimerization and protein stability of DJ-1.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PKAcα phosphorylates PARK7/DJ1 at the Thr154 residue. </LI> <LI> Disruption of Thr154 phosphorylation is linked to a lower stability. </LI> </UL> </P>

국립공원 3개 저수지에 서식하는 생태계교란 어종의 서식양상과 먹이생물, 어류상에 미치는 영향

박승철 ( Seung-chul Park ),이광열 ( Kwang Yeol Lee ),최광식 ( Kwang-seek Choi ),한미숙 ( Mee-sook Han ),고명훈 ( Myeong-hun Ko ) 한국어류학회 2021 韓國魚類學會誌 Vol.33 No.2

본 연구는 국립공원 내 생태계교란 어종 배스와 블루길의 도입에 따른 영향을 파악하기 위해 2020년 금계지, 삼가지, 내장지에서 조사를 실시하였다. 금계지에서는 7과 11종 1,221개체가 채집되었고, 배스는 우점종 (96.3%), 블루길은 소수 (0.3%) 서식하고 있었고, 배스는 주로 밀어 (IRI, 37.2%)와 잠자리목(25.6%), 뱀잠자리목 (11.6%), 배스 (7.0%) 등을 섭식하였다. 삼가지에서는 5과 10종 854개체가 채집되었고, 배스는 우점종(60.8%)으로 주로 새우류 (33.6%)와 잠자리목 (34.4%), 밀어(21.2%), 피라미 (6.1%) 등을 섭식하였다. 내장지에서는 7과 13종 1,075개체가 채집되었고, 블루길은 우점종 (38.1%), 배스는 우세종 (9.5%)으로 나타났으며, 블루길은 주로 물벼룩류 (IRI, 77.5%)와 파리목 (9.8%), 새우류 (4.0%) 등을 섭식하였고 배스는 주로 밀어 (73.3%)와 새우류 (21.2%) 등을 섭식하였다. 금계지와 삼가지의 배스는 10여 년 전에 도입된 것으로 추정되고, 도입 이후 대부분의 어류는 개체수가 급격히 감소하였다. 내장지의 블루길은 20여 년 전에 도입된 것으로 추정되며, 도입 이후 상대풍부도가 점점 증가하여 40%대에 도달하였고, 배스는 5~6년 전에 도입되었고 도입 이후 자생 어류의 종수와 개체수가 급격히 감소하였다. 마지막으로 국립공원 내 배스와 블루길의 서식현황 및 관리방안에 대해서 논의하였다. This study was conducted to elucidate the impact of invasive species, Micropterus salmoides and Lepomis macrochirus in Geumgyeji, Samgaji and Naejangji reservoirs of National Parks, Korea in 2020. In the Geumgyeji, 1,221 individuals of 11 species in 7 families were collected including M. salmoides (relative abundance, 96.3%) and L. macrochirus (0.3%), M. salmoides fed mainly on Rhinogobius brunneus (IRI, 37.2%), Odonata (25.6%), Megaloptera (11.6%), and M. salmoides (7.0%). In the results of Samgaji showed that 854 individuals of 10 species in 5 families were collected including M. salmoides (60.8%), and M. salmoides fed mainly on Decapoda (shrimp, 33.6%), Odonata (34.4%), R. brunneus (21.2%), and Zacco platypus (6.1%). In the Naejangji showed that 1,075 individuals of 13 species belonging to 7 families were collected including L. macrochirus (38.1%) and M. salmoides (9.5%), and L. macrochirus fed mainly on Branchiopoda (77.5%), Diptera (9.8%), Decapoda (4.0%) and M. salmoides fed mainly on R. brunneus (73.3%), Decapoda (21.2%). M. salmoides of Geumgyeji and Samgaji were apparently introduced more than a 10 years ago. The fish population declined rapidly since the introduction of M. salmoides. L. macrochirus of Naejangji was introduced more than 20 years ago, which increased its relative abundance to 40%. M. salmoides was introduced five to six years ago, and the fish species and population declined rapidly since the introduction of M. salmoides. Finally, we discussed the inhabitat status and management of M. salmoides and L. macrochirus in the National Park.

Gene cloning and utility phophorylation assay of a protein-fused substrate for a highly sensitive detection of cdc2 protein kinase using a radioisotope detection technique for the development of a protein biochip

Ko, Kyong-Cheol,Choi, Mi Hee,Park, Sang Hyun John Wiley Sons, Ltd. 2009 Journal of labelled compounds & radiopharmaceutica Vol.52 No.4

<P>The prototype of the cdc2 protein kinase in mammalian cells regulates its entry into mitosis by phosphorylating a group of key proteins in the major cell cycle transitions. In this study, using the mep45 gene encoding the 45 kDa major envelope protein (Mep45) of Selenomonas ruminantium, a rumen bacteria, a Mep45-fused substrate (PKTPKKAKKL-Mep45, MFS-cdc2) was cloned to detect the activity of cdc2 protein kinase. We report here on a strategy for the detection of a phosphorylation of a substrate catalyzed by cdc2 protein kinase by using a radioisotope detection technique. It is possible to constantly obtain a reasonable quantity of MFS-cdc2 for the cdc2 protein kinase assay and its cost can be as low as a synthesized peptide. Results of the study indicate that the Mep45-fused protein can be used effectively as a substrate for detecting the activity of cdc2 protein kinase and it can be used in developing a protein biochip for a high-throughput screening and also for studying protein–protein interactions. Copyright © 2009 John Wiley & Sons, Ltd.</P> <B>Graphic Abstract</B> <P>Gene cloning and utility phophorylation assay of a protein-fused substrate for a highly sensitive detection of cdc2 protein kinase using a radioisotope detection technique for the development of a protein biochip Kyong-Cheol Ko, Mi Hee Choi, Sang Hyun Park<SUP>*</SUP>Using the mep45 gene encoding the 45 kDa major envelope protein (Mep45) of Selenomonas ruminantium, a rumen bacteria, a Mep45-fused substrate (PKTPKKAKKL-Mep45, MFS-cdc2) was cloned to detect the activity of cdc2 protein kinase. We report here on a strategy for the detection of a phosphorylation of a substrate catalyzed by cdc2 protein kinase by using a radioisotope detection technique. Copyright © 2009 John Wiley & Sons, Ltd. <img src='wiley_img/03624803-2009-52-4-JLCR1579-gra001.gif' alt='wiley_img/03624803-2009-52-4-JLCR1579-gra001'> </P>

지역냉방 시스템용 초고효율 판형 열교환기 개발에 관한 연구

박재홍(Jae-Hong Park),임혁(Hyug-Lim),조성열(Sung-Youl Cho),강인성(In-Sung Kang),김정규(Jung-Kyu Kim),허인은(In-Eun Hur),고성규(Seong-Kyu Ko),곽승식(Sung-Sik Kwak),김종재(Jong-Jae Kim) 한국마린엔지니어링학회 2010 한국마린엔지니어링학회 학술대회 논문집 Vol.2010 No.10

Plate heat exchanger consists of thin, rectangular, pressed sheet metal plates that are sandwiched between full peripheral gaskets and clamped together in a frame. The frame has a fixed end-cover plate, fitted with the connecting ports, which is bolted together with a movable cover plate to hold the embossed plates in between; the top and bottom carrying bars allow proper alignment of the plate stacks. When compared with the well-established shell and tube heat exchangers, the plate heat exchanger shows a lot of advantages like high NTU values, compactness, low cost, multi duties and reduced fouling etc. Plate heat exchangers are often used to transfer heating or cooling water which is produced in the energy production( district heating or district cooling) facilities to residential areas and industrial parks. The district cooling systems of Middle East are the biggest market of plate heat exchanger, but the technical specifications for cooling system are different from the domestic one. To achieve its specifications, the new plate heat exchanger was developed, and its thermal performance results are introduced in this paper.

Generation of Transgenic Pigs Carrying a Construct for Endothelial Specific Expression of CD73

Keon Bong Oh,Bella Kim,Nayoung Ko,Dae-Jin Kwon,Dong-Hoon Kim,Gi-Sun Im,Jin-Ki Park,Seongsoo Hwang 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Despite of the absence of hyperacute rejection and acute humoral xenograft rejection, the organ graft of the a1,3-galactosyltransferase (GalT) gene knockouted (KO) and complement regulatory protein (CRP) expressing pig into a nonhuman primate is rejected by development of a thrombotic microangiopathy and/or a consumptive coagulopathy. Thus further introduction of genes to overcome the coagulation incompatibilities between pig and primate under GalT KO/CRP genetic background has been strongly suggested. CD73 (ecto-5'-nucelotidase) is an enzyme attached via a glycosyl phosphoinositol anchor to the extracellular membrane of endothelial cells, which catalyses the hydrolysis of adenosine triphosphate to adenosine. Loss of activity of CD73 results in activation and aggregation of platelets by a reduced capacity to convert nucleotides to adenosine. In previous study, we reported generation of GalT KO fibroblasts concurrently expressing membrane cofactor protein and produced cloned pigs by nuclear transfer of the fibroblast cells (1). In this study, we constructed a vector for expression of human CD73 under control of promoter of pig Icam2 gene expressed specifically at endothelial cells. This vector was introduced into porcine fibroblasts using the nucleofection technology, by which we had forty three fibroblasts clones carrying pIcam2- CD73 vector. Somatic cell nuclear transfer resulted in generation of two transgenic piglets survived.

불법 주정차 차량 단속을 위한 차량 검지 및 추적 기법

김승균,김효각,장동니,박상희,고성제,Kim, Seung-Kyun,Kim, Hyo-Kak,Zhang, Dongni,Park, Sang-Hee,Ko, Sung-Jea 한국전기전자학회 2009 전기전자학회논문지 Vol.13 No.2

본 논문은 불법 주정차 단속을 위한 정지 차량 검지 및 추적 기법을 제안한다. 제안하는 알고리즘은 크게 네 부분으로 구성되어 있다. 먼저, 입력 영상으로부터 움직이는 차량을 구분하기 위하여 향상된 코드북 물체 검지 알고리즘을 이용한 차량 검지 알고리즘을 제안한다. 두 번째로 차량의 기하학적 특징을 이용하여 차량이 아닌 물체는 제외시키는 전처리 기법을 사용한다. 그런 다음, 검지된 결과 차량들을 히스토그램 추적 기법과 칼만 필터를 결합한 추적 알고리즘을 이용하여 추적한다. 추적 결과를 더 정확하게 하기 위하여, 히스토그램 추적 결과를 칼만 필터의 측정 데이터로 사용한다. 마지막으로, 정지 차량 검지 알고리즘의 신뢰성 있고 정확한 성능을 위하여 실제 정지 카운터 (RSC)를 제안한다. 실험결과로부터 제안한 시스템은 복잡한 실제 도로 환경에서도 여러 차량을 동시에 추적할 수 있고, 정지 차량을 성공적으로 검지해냄을 확인한다. This paper presents a robust vehicle detection and tracking algorithm for supervision of illegal parking. The proposed algorithm is composed of four parts. First, a vehicle detection algorithm is proposed using the improved codebook object detection algorithm to segment moving vehicles from the input sequence. Second, a preprocessing technique using the geometric characteristics of vehicles is employed to exclude non-vehicle objects. Then, the detected vehicles are tracked by an object tracker which incorporates histogram tracking method with Kalman filter. To make the tracking results more accurate, histogram tracking results are used as measurement data for Kalman filter. Finally, Real Stop Counter (RSC) is introduced for trustworthy and accurate performance of the stopped vehicle detection. Experimental results show that the proposed algorithm can track multiple vehicles simultaneously and detect stopped vehicles successfully in the complicated street environment.

고등학생의 도덕적 판단능력과 자아개념과의 관계 연구

박영준,박고훈 木浦大學校 敎育硏究所 2001 교육연구 Vol.14 No.-

The purpose of this study is to find out the relations between moral judgement ability and self-concept of high school students. The findings of this study are as follows : 1.The moral judgement ability of high school students was on the fourth stage of Kohlberg's moral judgement stage. 2.The general high school students are higher than the industrial high school students in moral judgement ability. 3.The general high school students are higher than the industrial high school student in self-concept. 4.There are not any significant differences between moral judgement ability and self-concept.

전교조의 이념과 활동에 대한 중등교사의 인식에 관한 연구

박고훈,김익수 木浦大學校 敎育硏究所 2002 교육연구 Vol.15 No.-

전교조가 합법화된 상황속에서 전교조 교육이념의 인식정도를 알아보고, 교원노조법에 대해 교사들의 인식과 전교조 활동으로 인한 학교현장의 변화에 대한 교사들의 의견을 알아보고자 본 연구를 수행하였다. 본 연구에 따르면 교사들이 전교조에 가입한 동기는 교육제도의 모순을 개선하고 참교육실천에 관심이 있어서 이고 전교조의 교육이념에 대해 현장교사들은 대부분 꼭 필요한 이념이라고 생각하는 것으로 나타났으며, 전체의 반 이상의 교사들이 학습권을 침해하지 않는 범위내에서의 단체행동권과 교원노조의 교섭범위 및 활동이 법적으로 보장되어야 한다고 생각하였고, 교원노조의 정당가입과 정치활동에 대해서는 반반의 분포를 보였다. 마지막으로 전교조가 중점적으로 해야할 사업으로는 교육환경을 개선해야 한다는 의견이 지배적이었다. This study aims to examine the ideas and movement history of Korean Teacher's Union, to contemplate its influences on the teacher society and to consider the development objects and the roles to contribute toward the desirable teacher society. For the research, references about the ideas and movement history of Korean Teacher's Union and systems and organizations of teachers labor unions were examined and a questionnaire was used to investigate the teachers understanding of ideas and activities of Korean Teacher's Union. According to the results, most of the teachers joined in the association to improve inconsistencies of the education system. They are considering how they can apply the ideas of the association to the school education field. In addition, the right of collective action of the association should be admitted and all sorts of activities, which include the political activities and bargaining range of the association, should be guaranteed legally. The crises of public education collapse should be restored through improvement of educational environment and it should be the prior task of the association.