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Differentiation of Lymphocystis Disease Virus Genotype by Multiplex PCR
Kitamura Shin Ichi,Jung Sung-Ju,Oh Myung-Joo The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.2
Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease. The viruses have been divided into three genotypes (genotype I for LCDV-1, II for Japanese flounder isolates, and III for rockfish isolates) on the basis of major capsid protein (MCP) gene sequences. In this study, we developed a multiplex PCR primer set in order to distinguish these genotypes. We also analyzed the MCP gene of a new LCDV isolate from the sea bass (SB98Yosu). Comparison of sequence identities between SB98Yosu and eight Japanese flounder isolates, revealed identity of more than 90.1 % at nucleotide level and 96.5% at deduced amino acid level, respectively. Phylogenetic analyses based on the MCP gene showed that SB98Yosu belongs to genotype II, along with Japanese flounder isolates. Multiplex PCR based on the MCP gene allowed us to identify these genotypes in a simple and rapid manner, even in a sample that contained two genotypes, in this case genotypes II and III.
Kitamura, C.,Yasuda, Y.,Kobayashi, T.,Nomura, T.,Shimada, K. Asian Australasian Association of Animal Productio 1999 Animal Bioscience Vol.12 No.6
To evaluate direct and maternal effects on calf market weight (CMW) and carcass weight (CW) in Japanese Black cattle under an animal model, genetic parameters were estimated using 51,320 records of CMW and 11,944 records of CW, respectively. Direct and maternal heritabilities, and direct-maternal genetic correlation were estimated to be 0.22, 0.06 and 0.27 for CMW, and 0.23, 0.12 and -0.40 for CW, respectively. Correlation coefficient between maternal breeding values for CMW and CW was 0.521 for 157 sires appeared in both CMW and CW data sets. These results suggest that the maternal genetic effect on pre-weaning growth carries over to carcass weight. Maternal breeding values for both calf market weight and carcass weight could be used as the indicator traits of maternal ability in Japanese Black cattle.
Temperature - Dependent Micro Crack Healing Process
Kitamura, Kaxuhiro,Maksimov, Igor L .,Nishioka, Kazumi 대한금속재료학회(대한금속학회) 1998 METALS AND MATERIALS International Vol.4 No.6
The crack healing process is studied theoretically on the basis of the extended the Thomson-Obreimoff model. The system free-energy F as a function of the crack length l and temperature T is calculated using the self-consistent Einstein model. It is found that with the temperature increase, the energy barrier becomes lower and even disappears at the $quot;healing$quot; temperature T=T_h. It is demonstrated that the crack-lattice trapping in the Obreimoff model is controlled by the free-energy barrier height OF(T). The latter exhibits the universal power-law behavior. On the basis of the results obtained, the crack healing time is evaluated.
Rehabilitation Support Robot for Self-Standing-Up Training of Hemiplegic Stroke Patients
Mizuki Kitamura,Lin Han,Takahito Yamaji,Shunya Kaneko,Yoshifumi Morita,Hirofumi Tanabe 제어로봇시스템학회 2016 제어로봇시스템학회 국제학술대회 논문집 Vol.2016 No.10
The final goal of our research is to develop a rehabilitation support robot for self-standing-up training of hemiplegic stroke patients to restore normal standing-up motion. To improve the motor function of the patient’s paralyzed leg, a guided standing-up training is effective. The therapist controls the patient’s left/right load balance by pulling or pushing the patient’s waist during standing-up training. The purpose of this study was to clarify the patient’s waist movement guided by the therapist during the guided standing-up motion. For this purpose, we investigated the relationship between the patient’s waist movement and the left/right load balance during guided standing-up motion. From the analysis results, we found the ideal pattern of load balance ratio during guided standing-up training. Moreover, we found that the patient’s waist movement corresponded to the ideal pattern of load balance ratio. This movement trajectory is expected to be useful in designing a controller of a rehabilitation support robot.
( Hiroki Kitamura ),( Astryd Viandila Dahlan ),( Fumitake Takahashi ) 한국폐기물자원순환학회(구 한국폐기물학회) 2016 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2016 No.-
In Japan, municipal solid waste incineration (MSWI) fly ash is landfilled after immobilization treatment owing to high contents of heavy metals. Major treatment method is chelate treatment. Its immobilization mechanism is considered as complexation between chelating substances and heavy metals. Although mineralogical characteristics of MSWI fly ash are changed dramatically by secondary mineral formation in chelate treatment process, MSWI fly ash has been considered as homogeneous because fly ash is fine particles. However, micro-characteristics of fly ash might have non-negligible impact on leaching behavior of heavy metals if fly ash is heterogeneous. Therefore, this study investigated heterogeneity of raw and chelate-treated MSWI fly ash particles by elemental line profile analysis. Experimental samples are raw and chelate-treated MSWI fly ash collected from a Japanese MSWI facility plant equipped with stoker-type incinerator (incineration capacity : 250 Mg/d). Elemental mapping was conducted to investigate elemental distribution of fly ash particles by scanning electron microscope (SEM) equipped with energy dispersive X-ray analyzer (EDX). After elemental mapping, the surface of a fly ash particle was divided to 5 sections from the side to the other side horizontally. Line profile analysis was conducted at each divided section to analyze relative intensity of constituent elements. The coefficient of variation (C.V) of each element was calculated based on line profiles to evaluate heterogeneity in each particle. Elemental mapping showed that raw and chelate-treated MSWI fly ash particles consist mainly of Al, Ca, Cl, Na, O, and Si. In some cases, they contained other elements (K, Mg, and S) as well as heavy metals (e.g. Cr, Fe, Mn, Ti, and Zn). Calculated C.V. of major elements are shown in histogram (see Fig. 1). The C.V of Al, Na, O, and Si are mainly distributed in the range of 0 to 0.8. However, they often distributed around 1.0. Therefore, these elements seem to be heterogeneous in each particle. The C.V. of Ca are distributed in the range of 0 to 0.5. Therefore, Ca seems to be slightly heterogeneous in each particle. On the other hand, the C.V. of Cl are distributed widely. This means that Cl is very heterogeneous in each particle. As a result, these results suggest that a MSWI fly ash particle is heterogeneous although it has been considered as homogeneous without sufficient observations. In addition, its heterogeneity might have non-negligible impact on leaching behaviors of heavy metals. Line profile analysis is useful for micro-scale characterization of MSWI fly ash particles. However, only 51 particles were analyzed in this study. Therefore, further observations are required to homogenize experimental results.
Cervical nerve roots and the dural sheath: a histological study using human fetuses near term
Kei Kitamura,Masahito Yamamoto,Yoshinosuke Hirota,Noriyuki Sato,Toshimasa Machida,Noboru Ishikawa,Hitoshi Yamamoto,Gen Murakami,Shinichi Abe 대한해부학회 2020 Anatomy & Cell Biology Vol.53 No.4
We have previously reported that the thoracolumbar posterior nerve root shows a tortuous epidural course, based on studies of human fetuses near term. For comparison with the cervical nerve, examinations were conducted using frontal, sagittal and horizontal sections of cervical vertebrae from 22 fetuses at 30-38 weeks of gestation. The cervical nerve root showed a short, straight and lateral course near the zygapophysial joint. Multiple rather than single bundles of the cervical posterior root seemed to account for the majority of sensory nerve fibers innervating the upper extremity. Fasciculation of rootlets was evident near the thoracolumbar spinal cord, whereas it was seen in the dural pocket at the nerve exit from the dural sac although both sites were subdural. As in the thoracolumbar region, the nerve sheath was continuous with the dura mater and independently surrounded each of the anterior and posterior roots. Radicular arteries were few in the cervical region. In 2 of the 22 fetuses (31 weeks and 33 weeks), there was a segmental, unilateral abnormality of nerve rootlet fasciculation where the dorsal root ganglion was located lateral or peripheral to the intervertebral region. Long nerve roots running inferiorly are a necessary adaptation to the delayed and marked growth of the thoracolumbar vertebral column. In children, the cervical nerve roots are likely to be affected by movement or dislocation of the vertebrae. The segmental abnormality of the cervical nerve root may be linked to rare variations in the brachial plexus.