방사선조사가 당뇨 백서의 측두하악관절에 미치는 영향

안기동,황의환,이상래 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.2

Purpose : To investigate the histopathological changes in the temporomandibular joint in streptozotocin-induced diabetic rat following irradiation. Materials and Methods : Sprague-Dawley rats weighing about 250 gm were divided into three groups: control, diabetic, and diabetic-irradiated groups. Diabetes mellitus was induced in the rats by injecting streptozotocin. Rats in the control group were injected with citrate buffer only. After 5 days, the head and neck region of the rats in diabetic- irradiated group were irradiated with single absorbed dose of 10 Gy. The rats were killed at 1, 3, 7, 14, 21, and 28 days after irradiation. The specimen including the temporomandibular joint were sectioned and observed using a histopathological method. Results : In the diabetic group, severe bone resorption in the mandibular condyle was observed throughout the period of experiment. Necrosis of bone marrow and trabeculae was observed at 28 days after diabetic state. Atrophy and fibrosis in the retrodiscal tissue was gradually progressed during the time of the experiment. In the diabeticirradiated group, severe bone resorption in the mandibular condyle was observed during the early experimental phases, but regeneration of bone marrow was initiated at 14 days after diabetic state and irradiation. Also, calcification of abnormal trabeculae was observed at 28 days after diabetic state and irradiation. The retrodiscal tissue was degenerated in the early experimental phases, but it had been gradually regenerated during the experimental time. Conclusion : This experiment suggests that bone resorption and degeneration in the mandibular condyle are caused by the induction of diabetes, and abnormal bone formation is induced after irradiation in diabetic state.

Reconstruction of Rabbit Corneal Epithelium on Lyophilized Amniotic Membrane Using the Tilting Dynamic Culture Method

Ahn, Jae-Il,Lee, Doo-Hoon,Ryu, Yang-Hwan,Jang, In-Keun,Yoon, Mun-Young,Shin, Youn Ho,Seo, Young-Kwon,Yoon, Hee-Hoon,Kim, Jae-Chan,Song, Kye-Yong,Yang, Eun-Kyung,Kim, Ki-Ho,Park, Jung-Keug Blackwell Publishing Inc 2007 Artificial Organs Vol.31 No.9

<P>Abstract: </P><P>Rabbit corneal epithelium was reconstructed using tilting dynamic culture with a self-manufactured, amniotic membrane (AM) supporter and a lyophilized amniotic membrane (LAM). Rabbit corneal epithelial (RCE) cells were cultured and cryopreserved after isolation from the limbus. The second- and third-passage RCE cells were plated onto the epithelial side of the LAM of Ahn's AM supporter. Two days later, the air–liquid interface culture was maintained with third-passage RCE cells for 6 days and second-passage corneal epithelial cells for 9 days. The average viability of thawed RCE cells, assessed using trypan blue dye exclusion, was 77.42%. The reconstructed corneal epithelium was characterized by histological (hematoxylin and eosin) and immunohistochemical staining (proliferating cell nuclear antigen) for light microscopy, and by reverse transcriptase-polymerase chain reaction, glucose assay, and transmission electron microscopy. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the increase in cell proliferation and differentiation caused by the tilting dynamic culture, as opposed to static culture. Tilting dynamic culture was useful for the reconstruction of the epithelium using easily damaged epithelial cells and resulted in more stratum cell layers. Moreover, cytokeratin (CK3) mRNA expression in tilting dynamic cultured third-passage RCE cells seeded onto AM was greater than in static cultured third-passage RCE cells. The morphology of the reconstructed corneal epithelium on LAM by tilting dynamic culture for 9 days resembled that of the skin epidermis. This was thought to be because the tilting dynamic culture not only accelerated the proliferation and differentiation of cells by physical or mechanical stimulation, but also ensured that the supply of medium was delivered to the basal cells more efficiently. Thus, the reconstruction of the corneal epithelium using LAM and tilting dynamic culture was considered to be a good in vitro model for autologous or allogeneic transplantation of corneal epithelium and skin epidermis in patients with damaged epithelia. </P>

방사선조사가 Streptococcus mutans에 미치는 영향

안기동,김규태,최용석,황의환 대한구강악안면방사선학회 2007 Imaging Science in Dentistry Vol.36 No.1

Purpose : To observe direct effect of irradiation on cariogenic Streptooccus mutans. Materials and Methods : S. mutans GS5 was exposed to irradiation with a single absorbed dose of 10, 20, 30, and 40 Gy. Viability and changes in antibiotic sensitivity, morphology, transcription of virulence factors, and protein profile of bacterium after irradiation were examined by pour plate, disc diffusion method, transmission electron microscopy, RT-PCR, and SDS-PAGE, respectively. Results : After irradiation with 10 and 20 Gy, viability of S. mutans was reduced. Further increase in irradiation dose, however, did not affect the viability of the remaining cells of S. mutans. Irradiated S. mutans was found to have become sensitive to antibiotics. In particular, the bacterium irradiated with 40 Gy increased its susceptibility to cefotaxime, penicillin, and tetracycline. Under the transmission electron microscope, number of morphologically abnormal cells was increased as the irradiation dose was increased. S. mutans irradiated with 10 Gy revealed a change in the cell wall and cell membrane. As irradiation dose was increased, a higher number of cells showed thickened cell wall and cell membrane and lysis, and appearance of ghost cells was noticeable. In RT-PCR, no difference was detected in expression of gtfB and spaP between cells with and without irradiation of 40 Gy. In SDS-PAGE, proteins with higher molecular masses were gradually diminished as irradiation dose was increased. Conclusion : These results suggest that irradiation affects the cell integrity of S. mutans, as observed by SDS-PAGE, and as manifested by the change in cell morphology, antibiotic sensitivity, and eventually viability of the bacterium. (Korean J Oral Maxillofac Radiol 2007; 37: 35-43)

음향방출 및 초음파시험을 이용한 CFRP 시험편의 파괴 거동 해석

안석환,남기우 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.3

단조인장시험하에서 CFRP적층재의 손상과정을 음향방출과 초음파시험에 의해 평가하였다. 시험편으로부터의 음향방출신호의 진폭 분포는 기지재 균열(matrix crack), 박리(debonding), 섬유 pull-out 및 섬유 파단(fiber fracture)과 같은 CFRP에서의 파괴기구를 분석하는데 도움을 주며, 초음파 진폭감쇠의 특성 또한 각각의 파괴기구를 분석하는데 유용하다. 여러종류의 CFRP시험편을 사용하여 음향방출신호와 초음파신호의 진폭감쇠를 조사하였다. 끝으로 하중제하중(loading-unloading) 시험이 초음파내의 진폭비에 대해 felicity effect와 감쇠를 조사하기 위해서 수행되었다. 전실험에 걸쳐 초음파신호의 진폭감쇠와 음향방출 파라미터(parameter)들은 동시에 검출되었다. 이로써 음향방출과 초음파시험의 두 파라미터들이 CFRP에서의 파괴기구를 분석하기 위해 효과적으로 사용될 수 있음이 확인되었다. Damage process of CFRP laminates under monotonic tensile test was characterized by the correlation between Acoustic Emission(AE) and Ultrasonic Test(UT). The amplitude distribution of AE signal from a specimens is an aid to the determination of the extent of the different fracture mechanism such as matrix crack, debonding, fiber pullout and fiber fracture as load is increased. In addition, the characteristics of ultrasonic amplitude attenuation are useful for analysis of the different type of fracture mechanism. Different orientation of carbon fiber reinforced plastic specimens were used to investigate the AE amplitude range an ultrasonic amplitude attenuation. Finally, loading-unloading tests were carried out to check Felicity effect. During the tests, ultrasonic amplitude was investigated at the same time and compared with AE parameters. The result showed that two parameters of both AE and UT could be effectively used for analysis of fracture mechanism in CFRP laminates.

STS316L 모재 및 용착부의 열처리에 따른 초음파 특성

남기우,박소순,안석환 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.3

The degradation characteristics of the base metal and weld of STS316L were investigated by the experimental method of ultrasonic exposure with parameters such as ultrasonic velocity, attenuation factor and frequency. Through this research, degradation characteristics could be evaluated by the combinations of experimental ultrasonic parameters. According to the increase of grain sizes, standard deviation and ferrite contents, the ultrasonic velocities and frequencies were decreased, while the ultrasonic attenuation factors were increased. This results can be to predict the welded structures life time assesmant according to the aged degradation and damage without disjointing or stopping in-service.

신생검증례에 의한 사구체신질환의 분석

최두환,구시선,배승훈,박병현,안선호,송주흥 圓光大學校 醫科學硏究所 1996 圓光醫科學 Vol.12 No.2

To elucidate the incidences and the clinical characteristics of glomerular diseases in our hospital, we reviewed the clinical records and histological findings of the 117 cases, which were diagnosed as one of the glomerular disease in renal biospy for about 6 years since 1991. In these 117 cases, 74 cases (63.2 %) fell into primary glomerulonephritis(GN), 18 cases (15.4%) into secondary GN, 2 cases (1.7 %) into end stage kidney, 4 cases (3.4%) into tubulointerstitial disease and the biopsy samples were inadequate for diagnosis in 19 cases. In primary glomerular diseases, most common pathologic diagnosis was IgA nephropathy (36.5 %}, followed by minimal change nephrotic syndrome (29.7%). The incidence of IgA nephropathy would be increased if we had biopsied every asymptomatic urinary abnormality (AUA) patients. Systemic lupus erythematosus (SLE) . hepatitis B accounted for most of secondary GN in our cases. One case of progressive scleroderma was included in this secondary GN. Minimal change nephrotic syndrome (22 cases) was the most common cause of nephrotic syndrome, followed by membranous GN (9 cases) and IgA nephropathy (8 cases). 2 cases of primary amyloidosis were presented with nephrotic syndrome. Nephrotic syndrome as presenting symptom in our IgA nephropathy patients was higher than in other studies, which may be due to indication bias for renal biopsy. We suggest that renal biopsy should be done in adult nephrotic syndrome and in SLE, because various kind of glomerular diseases can develop nephrotic syndrome in adult, renal biopsy can affect the treatment and prognosis in each patients and renal biopsy also determines the extent of renal involvement in SLE on which the treatment depends. In AUA urinary patients, renal biopsy could be reserved for the patients who show declining of GFR or rising of proteinuria. Because IgA nephropathy account for most of AUA. and there is no specific treatment for IgA nephropathy, and renal biopsy in those cases is helpful in ruling out the patients who need no treatment.

사비의 교정에 있어서 내시경의 이용

박대환,김태모,이재욱,송철홍,한동길,안기영 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.1

Owing to the complexity of the deviated structures and the septal deformities accompanying the external nasal deformities, the complete correction of the deviated nose is regarded as a difficult one. The approach to the nasal bone and osteotomy in classic corrective rhinoplasty is almost blind technique, where the results depends on the feeling by surgeon's hand. To overcome these drawback, endoscopic-assisted corrective rhinoplasty and septoplasty performed for 8 cases of deviated nose between January 1996 and May 1997. Average follow-up period was 10 months. All patients were evaluated by symmetrical nasal pyramid, recurrence of the bony deflection and septal deviation. The postoperative courses were satisfactory in most of cases with few complications. It appears that endoscopic control during corrective rhinoplasty and septoplasty is a big step toward obtaining better results in bony and cartilage resection with extreme precision under monitor control and magnification. This technique is not an open approach but permits one to see more of the nasal skeleton and bony septum what is causing deformity, and the immediate effect of the corrective measures used. The use of endoscope in corrective rhinoplasty will provide expanded field of vision, direct manipulation of lesions, and better aesthetic an6 functional results.

포도 Delaware의 無核果生産을 위한 Streptomycin 利用試驗(II)

裵基煥,安相烈 진주산업대학교 1986 論文集 Vol.24 No.-

前 試驗에 이어 SM混用區의 GA_3 濃度를 50ppm으로 높이고 處理時期의 폭을 滿開前 18, 16, 14, 12, 10일로 하여 GA_3 單用區의 대체여부를 試驗한 結果는 다음과 같다. 1. 滿開期는 兩主區 모두 無處理보다 2~3일 빨랐다. 2. 熟期는 兩主區 모두 無處理보다 25일 정도 早熟되었고 單用區가 混用區에 비하여 1~2일 早熟되었으며 各細區間에 處理時期가 빠를수록 1~2일 정도 早熟되는 경향이 있었다. 3. 無核化率은 混用區는 40개區 모두 有核粒이 없었고, 單用區는 40개區 中 19개區에서 1~6개의 有核粒이 나타났다. 4. 收穫果房狀態는 單用區가 混用區에 比해서 1%水準 以上의 有意差로, 果房長은 길고 5%水準 以上의 有意差로, 平均 果粒重은 무거웠다. The studies were conducted continuously following previous test to determine the possibility of utilization with streptomycin for seedless berries production in Delaware grapes. The main plots which were applied with different chemicals(combind solution of GA_2(50ppm)+SM(200ppm), GA_3(100ppm) alone solution) as pre-bloom application and five split plots according to days applied before full bloom were designed. The results obtained were as follows; 1. The days of full bloom in both main plots was accelerated by two to three days compared with that in the untreatment. 2. The day of maturity showed tendency to delay one to two days in combined solution plot compared with that in alone solution plot. 3. In all combined solution plots, seed-holding berry was not found, but, in alone solution plots, they were found in nineteen out of forty plots. 4. The length of cluster harvested in combined solution plot was short and mean weight of berry was light compared with that in alone solution plot

은행잎 플라보놀배당체에 대한 셀루라제류의 영향

배기환,민병선,백흠영,안병준 충남대학교 암연구소 1991 癌共同硏究所 硏究誌 Vol.1 No.1

The extractability and stability of ginkgoflavonolglycosides under presence of several cellulase preparations were investigated. The enzymes used were macerosin, cellulase C and cellulase NC. The content variation of the glycosides was measured with HPLC method, using caffeic acid as an internal standard. The methanol extract of ginkgo leaf, containing the total flavonolglyco-sides of 4.46%, was used for the content comparison. By extraction with the enzymes, each or mixed, the peak levels of all the glycosides began to decrease after 1 or 2 hours. After 24 hour extraction, most of the glycosides were degraded to minor components. The flavonolglycosides in ginkgo leaf were also hydrolysed simply by the water extraction. After 24 hour extraction with water at 40°C, the peak levels of major glycosides were distinctly decreased. Rutin was hydrolysed by enzyme treatment or by ginkgo leaf itself. As a result, it was concluded that the commercially available cellulases and the ginkgo leaf itself contain the activities of β-glycosidase and α-rhamnosi-dase. Kaempferol-3-0-(6' -0-p-coumaroylglucosyl)-rhamnoside and four other ginkgo flavonolglycosides were not hydrolysed under the same condition."

내시경을 이용한 관골궁골절 정복술

박대환,이재욱,장경수,송철홍,한동길,안기영 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.1

Although the zygomatic arch can be employed as a key landmark to the accurate alignment of a displaced zygoma fracture, it has been traditionally avoided because of the need for a bicoronal incision. Exposure of the zygomatic arch by means of the conventional bicoronal incision has several possible disadvantages, including an increased risk of blood loss, alopecia, loss of sensation posterior to the incision, and traction palsy of the facial nerve. Endoscopic-assisted exposure of a zygomatic arch can largely obviate the disadvantages of a bicoronal incision and yield this site accessible to reduction and internal fixation in the routine treatment of displaced zygoma fractures. 9 cases of endoscope-assisted open reduction of zygomatic arch fracture and 3 cases of internal fixation of a moderately displaced zygoma fracture are presented. To accomplish this technique, a rigid 4 mm, 30 degree down-angled endoscope, trocar and cannula, endoscopic forehead lift instrument were used. The postoperative courses were satisfactory with few complications. The use of endoscope in the treatment of zygoma reduction provided an expanded field of vision, direct manipulation of lesions, minimal postoperative scar. In particular, exposure and fixation of the zygomatic arch were performed without the need for a bicoronal incision.