Effects of Supplementary Composts on Microbial Communities and Rice Productivity in Cold Water Paddy Fields

( Kai Zhi Xie ),( Pei Zhi Xu ),( Shao Hai Yang ),( Yu Sheng Lu ),( Rui Ping Jiang ),( Wen Jie Gu ),( Wen Ying Li ),( Li Li Sun ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.5

Cold water paddy field soils are relatively unproductive, but can be ameliorated by supplementing with inorganic fertilizer from animal waste-based composts. The yield of two rice cultivars was significantly raised by providing either chicken manure or cow dung-based compost. The application of these composts raised the soil pH as well as both the total nitrogen and ammonium nitrogen content, which improved the soil’s fertility and raised its nitrification potential. The composts had a measurable effect on the abundance of nitrogencycling- related soil microbes, as measured by estimating the copy number of various bacterial and archaeal genes using quantitative real-time PCR. The abundance of ammonia oxidizing archaea and bacteria was markedly encouraged by the application of chicken manure-based compost. Supplementation with the composts helped promote the availability of soil nitrogen in the cold water paddy field, thereby improving the soil’s productivity and increasing the yield of the rice crop.

Antibacterial characteristics of glycinin basic polypeptide against Staphylococcus aureus

Jie Yang,Gui-Jin Sun,Ying-Qiu Li,Kai-Yu Cui,Hai Zhen Mo 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

This paper aims to study the antibacterial action of glycinin basic polypeptide (GBP) on Staphylococcus aureus (S. aureus). Herein, the minimum inhibitory concentration (MIC) of GBP against S. aureus was 0.2 mg/mL. Atomic force microscopy (AFM) imaging showed that GBP seriously damaged the morphology of the S. aureus cells. GBP (0.8 mg/mL) enhanced the relative release of β- galactosidase to 25.48% when compared to the control. The activity of the respiratory-chain dehydrogenase of S. aureus decreased with increasing GBP concentration. GBP could cause a leakage of intracellular substances. Additionally, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that S. aureus bacterial proteins decreased in response to the time period of treating the bacterial cells with GBP. These results indicate that GBP could remarkably inhibit S. aureus and is, therefore, a potential food preservative.

Trichoderma biodiversity in major ecological systems of China

Kai Dou,Jinxin Gao,Chulong Zhang,Hetong Yang,Xiliang Jiang,Jishun Li,Yaqian Li,Wei Wang,Hongquan Xian,Shigui Li,Yan Liu,Jindong Hu,Jie Chen 한국미생물학회 2019 The journal of microbiology Vol.57 No.8

An investigation of Trichoderma biodiversity involving a large-scale environmental gradient was conducted to understand the Trichoderma distribution in China. A total of 3,999 isolates were isolated from forestry, grassland, wetland and agriculture ecosystems, and 50 species were identified based on morphological characteristics and sequence analysis of genetic markers. Trichoderma harzianum showed the largest proportion of isolates and the most extensive distribution. Hypocrea semiorbis, T. epimyces, T. konilangbra, T. piluliferum, T. pleurotum, T. pubescens, T. strictipilis, T. hunua, T. oblongisporum and an unidentified species, Trichoderma sp. MA 3642, were first reported in China. Most Trichoderma species were distributed in Jilin and Heilongjiang Provinces in northeast China and the fewest were distributed in Qinghai Province. Based on the division of ecological and geographic factors, forestry ecosystems and low-altitude regions have the greatest species biodiversity of Trichoderma.

Content of Dietary Fiber in Zizania Galls and Its Reaction to Different Types of Fertilizer

Jie-zeng Jiang,Kai-feng Huang,Yu-lian Qin,Kai Yang,Bei-sheng Cao 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Investigation of the Effect Mechanism of Cutting Sequence on Spiral Milling of CFRP/Ti Laminates Under Ultrasonic-Assisted Conditions

Kai Yang,Jie Pan,Ziyang Cao,Yiwen Zhu 한국정밀공학회 2024 International Journal of Precision Engineering and Vol.25 No.4

The hole-making mechanism of carbon fiber reinforced plastic (CFRP)/titanium alloy stacks is different from that of monolayer materials. In order to reveal the effects of different cutting sequence strategies on the hole-making quality and machining efficiency of the stacked materials, valuable insights are provided for the design of the stacking machining process and the selection of the most suitable cutting sequence. The researchers conducted ultrasonic vibration-assisted helical milling and conventional helical milling experiments with different milling parameters, and explored the basic influence mechanisms of CFRP → Ti and Ti → CFRP on the hole-making in both ways. The results show that different cutting sequences have significant effects on the axial force and chip morphology changes during CFRP/Ti stack machining. The dimensional accuracy and consistency of the holes cut from the Ti side are better, and the quality of the entrance and exit from the Ti side under the ultrasonically assisted condition is close to the quality level of the holes cut from the CFRP side under the normal helical milling, and the ultrasonically assisted condition plays a good role in improving the quality of the holes made. In addition, the tearing coefficients of CFRP hole entry/exit in both machining modes increased with the increase of feed per tooth in tangential direction and decreased with the increase of spindle speed. The tearing coefficients of entry/exit holes in CFRP direction were smaller than those in titanium direction.

Identification of HDAC9 as a viable therapeutic target for the treatment of gastric cancer

Kai Xiong,Hejun Zhang,Yang Du,Jie Tian,Shigang Ding 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Histone deacetylase inhibitors (HDACis) are a new class of anticancer drugs confirmed to have good therapeutic effects against gastric cancer (GC) in preclinical experiments, but most HDACis are non-selective (pan-HDACis), with highly toxic side effects. Therefore, it is necessary to screen HDAC family members that play key roles in GC as therapeutic targets to reduce toxic side effects. In this study, we evaluated the targeting specificity of the HDACi suberoylanilide hydroxamic acid (SAHA) for GC via fluorescence molecular imaging (FMI). In vitro FMI results showed that SAHA had higher binding affinity for GC cells than for normal gastric cells. In vivo FMI of gastric tumor-bearing mice confirmed that SAHA can be enriched in GC tissues. However, there was also a high-concentration distribution in normal organs such as the stomach and lungs, suggesting potential side effects. In addition, we found that among the HDAC family members, HDAC9 was the most significantly upregulated in GC cells, and we verified this upregulation in GC tissues. Further experiments confirmed that knockdown of HDAC9 inhibits cell growth, reduces colony formation, and induces apoptosis and cell cycle arrest. These results suggest that HDAC9 has an oncogenic role in GC. Moreover, HDAC9 siRNA suppressed GC tumor growth and enhanced the antitumor efficacy of cisplatin in GC treatment by inhibiting the proliferation and inducing the apoptosis of GC cells in vitro and in vivo. Our findings suggest that the development of HDAC9-selective HDACis is a potential approach to improve the efficacy of chemotherapy and reduce systemic toxicity.

Antibacterial characteristics of glycinin basic polypeptide against Staphylococcus aureus

Yang, Jie,Sun, Gui-Jin,Li, Ying-Qiu,Cui, Kai-Yu,Mo, Hai Zhen 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

This paper aims to study the antibacterial action of glycinin basic polypeptide (GBP) on Staphylococcus aureus (S. aureus). Herein, the minimum inhibitory concentration (MIC) of GBP against S. aureus was 0.2 mg/mL. Atomic force microscopy (AFM) imaging showed that GBP seriously damaged the morphology of the S. aureus cells. GBP (0.8 mg/mL) enhanced the relative release of ${\beta}$-galactosidase to 25.48% when compared to the control. The activity of the respiratory-chain dehydrogenase of S. aureus decreased with increasing GBP concentration. GBP could cause a leakage of intracellular substances. Additionally, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that S. aureus bacterial proteins decreased in response to the time period of treating the bacterial cells with GBP. These results indicate that GBP could remarkably inhibit S. aureus and is, therefore, a potential food preservative.

An uncertainty representation based sampling method for metamodeling in auto-motive design applications

Junqi Yang,Zhenfei Zhan,Kai Zheng,Chong Chen,Jie Hu,Ling Zheng 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.10

Meta-model is frequently employed as the surrogate of high-fidelity finite element models in computer aided engineering. It helps to achieve the trade-off between computational efficiency and predictive accuracy. To improve the predictive capability of meta-models, we developed an uncertainty representation based sampling method to schedule Design of experiment (DOE) for meta-modeling. Several datasets were first generated through a modified Bootstrap method for datasets acquisition, then the influence of the input uncertainty on the output was quantified as weighting factors. The weighting factors were used to integrate the represented distributions into a single one for further sampling. Finally, the sampling results then served as the elements of the DOE matrix to construct meta-models. The proposed method was demonstrated through an analytical case and a real-world vehicle crashworthiness design problem.

MicroRNA-146a Enhances Helicobacter pylori Induced Cell Apoptosis in Human Gastric Cancer Epithelial Cells

Wu, Kai,Yang, Liu,Li, Cong,Zhu, Chao-Hui,Wang, Xin,Yao, Yi,Jia, Yu-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Helicobacter pylori (H. pylori) infection induces apoptosis in gastric epithelial cells, and this occurrence may link to gastric carcinogenesis. However, the regulatory mechanism of H. pylori-induced apoptosis is not clear. MicroRNA-146a has been implicated as a key regulator of the immune system. This report describes our discovery of molecular mechanisms of microRNA-146a regulation of apoptosis in human gastric cancer cells. We found that overexpression of microRNA-146a by transfecting microRNA-146a mimics could significantly enhance apoptosis, and this upregulation was triggered by COX-2 inhibition. Furthermore, we found that microRNA-146a density was positively correlated with apoptosis rates in H. pylori-positive gastric cancer tissues and intratumoral microRNA-146a density was negatively correlated with lymph node metastasis among H. pylori-positive gastric cancer patients. Understanding the important roles of microRNA-146a in regulating cell apoptosis in H. pylori infected human gastric cancer cells will contribute to the development of microRNA targeted therapy in the future.

Two-dimensional lamellar phosphogypsum/polyethylene glycol composite PCM: Fabrication and characterization

Jinfen Lou,Kai Zhang,Shuhao Qin,Yang Lei,Yufei Liu,Min He,Jie Yu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

In this work, a nanocomposite phase change material (PCM) has been designed by combining twodimensionallamellar anhydrous calcium sulfate with polyethylene glycol (PEG). We report a facile strategyto controllably fabricate two-dimensional lamellar anhydrous calcium sulfate (LAH) with the averagethickness of 28.63 nm from phosphogypsum (PG) through ethylenediamine tetraacetic acid disodium(Na2EDTA) induction in glycerol and ethylene glycol solutions at 98 C. The obtained 2D lamellarCaSO4 was a slit-type mesoporous material stacked by the nanosheet of calcium sulfate. It has a specificsurface area of 70.02 m2/g, which is 10 times larger than phosphogypsum. Na2EDTA acts as a crystalhabit-directing agent to regulate crystal morphology through nonclassical nanoparticle-mediated crystallizationprocesses, resulting in the crystalline morphology tending to be lamellar. Lamellar anhydrouscalcium sulfate phase change composites (LAHPCMs) were prepared with 2D lamellar anhydrous nano-CaSO4 and polyethylene glycol (PEG). The LAHPCMs had a high latent heat storage capacity (92.99 J/g). Lamellar anhydrous calcium sulfate phase change composites have good thermal stability and durability,structure stability, and good liquid leakage resistance. These results provide the possibility for phosphogypsumto be used for energy storage and thermal insulation.