동물(動物)바이러스의 새로운 분류법(分類法)

정종식 ( Jong Sik Jyeong ) 한국가축위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.1

사라리 축우페사의 원인에 관한 연구

정종식 ( Jong Sik Jyeong ),박노찬 ( No Chan Park ),김정화 ( Jung Hwan Kim ),김영환 ( Young Hoan Kim ),조광현 ( Kwang Hyun Cho ),조민희 ( Min Hee Cho ),손재권 ( Jae Kweon Son ),김영욱 ( Young Wook Kim ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.4

This study was conducted to investigate the epidemiological, clinicopathological, microbiological, pathological observations and other tests from sudden death in feedlot cattle at the region of Sarari in Korea during the period from 1994 to 1999. Massive or sporadic occurrence of sudden death has been observed in 101 heads of 47 farmhouse. There were 20.8% in spring, 29.7% in summer, 16.8% in autumn, 32.7% in winter, and 62.3% in reproductive, 27.7% in growing, 5.0% in beef cattle, 5.0% in calf in prevalence of sudden death in cattle. Enterotoxemia(88.0%), pneumonia(3.5%), intestinal diarrhea(3.5%), liver abscess(1.5%) and indigestion(1.5%) were detected from 67 heads of sudden death cattle. In clinical observations, cattle were generally died of sudden recumbency with convulsions followed anorexia, depression, ataxia, muscular tremor, tachycardia and dyspnea without any premonitory symptoms. Epidemiological surveys showed no evidence that other factors such as pesticide, insecticide, fertilizer, chemical drugs and those of others caused sudden death. Macroscopically, there were coagulation disorders of blood, congestion, edema and haemorrhage of lung, congestion and haemorrhages, watery and blood-tinged contents of small intestine. Histopathologically, we observed pulmonary congestion and haemorrhage, necrotic intestinal mucosa accompanied with haemorrhage and congestion, and also increased globule leukocytes between bronchial epithelia with mild pneumonia. Clinicopathologically, only elevation of blood glucose and aspartate aminotransferase (AST) was detected. Magnesium and calcium deficiency were not detected, but parasites were detected highly in normal and dead cattles. Microbiologically, Clostridium(Cl) perfringens were detected from small intestinal contents of 94%(63/67) of sudden death cattle and 51%(51/101) of slaughter cattle, and the population were 10(6-8)cfu/mℓ after 16~32 hours. Consequently, it was proved that the cause of death in cattle was enterotoxemia. Pathogenic test of mouse and goat inoculated with Cl perfringens type A toxin has been demonstrated as similar observation to natural cases. In antimicrobial susceptibility test, ampicillin, bacitracin, polymycin, cephalothin, penicillin, choramphenicol, erythromycin, tetracycline were highly susceptible, and amikacin, gentamicin, kanamycin, neomycin, streptomycin, sulfamethoxine, sulfamethazine were resistant. Cl perfringens were resisted for 4 hours in 3% formalin, 20 minutes in 4% phenol, 20 minutes in 05% mercuric chloride and 40 minutes in 0.1% sodium hydroxide, respectively. The useful method to prevent from occurrance of enterotoxemia in feedlot cattle was a dietary administration of antibiotics and miyari acid.

경북지역에서 수거된 식육의 Listeria sp 오염실태 조사

조종숙 ( Jong Suk Cho ),김유정 ( Yoo Jung Kim ),김성숙 ( Seong Suk Kim ),도재철 ( Jae Cheul Do ),김석한 ( Suk Hwan Kim ),이창우 ( Chang Woo Lee ),김인성 ( In Sung Kim ),정종식 ( Jong Sik Jyeong ) 한국가축위생학회 2000 韓國家畜衛生學會誌 Vol.23 No.4

Nowadays we continue to face challenges to the safety of our foods. It seems clear that contaminated meats provide the major route of listeria monocytogenes transmission from the environment to humans. L monocytogenes is the most important species associated with disease in humans among the listeria sp. The current incidence of symptomatic listeriosis caused by L morzocytogenes is uncertain. Although the number of reports in the literature on listeriosis are increasing, it is likely that they are actually unrecognized or underreported because of a lack of awareness on the part of some laboratory workers who fail to isolate or identify these organisms. To get the information of sanitary development, we survey various meats (beef, pork, etc) in Kyongbuk area. Samples were collected from local meat shop at Kyongbuk area. Total sixty six case were isolated and identified from one hundreds and seven samples. L innocua was the most abundant in listeria sp. Among 66 isolates, the number of isolated L innocua was 43(65.2%). The numbers of isolated L murrayi, L welshimeri, L monocytogenes and L seeligeri were 12 (18.2%), 7 (10.6%), 3 (4.5%) and 1 (1.5%), respectively, but L grayi, L ivanovii were not isolated.

Alcohol-induced hepatic fibrosis in pig

Lee, Chang-Woo,Jyeong, Jong-Sik,Lee, Cha-Soo,Jeong, Kyu-Shik The Korean Society of Veterinary Service 2003 韓國家畜衛生學會誌 Vol.26 No.4

A number of toxicants have been incriminated as a causing hepatic disease. Among many detrimental injury, alcohol has been noted for hepatitis, fatty liver, fibrosis, and hepatic cirrhosis. The purpose of this study was to develop animal model for hepatic fibrosis in pigs fed ethanol, and to search for a new anti-fibrogenic agent via this model. Twelve male Landrace pigs were divided into 3 groups of 4 animals each. Group 1, 2 and 3 were fed with active ceramic water only, ceramic water + liquid diet containing 15% ethanol and normal tap water + liquid diet containing 15% ethanol for 12 weeks, respectively. At week 12, all pigs were immediately sacrificed for collection each tissue and blood. Serologically, serum ALT and AST levels were significantly reversed in group 2, as compared to group 3. They were normal range in pigs of group 1. Microscopically, macrovesicular lipid droplets and moderate hepatocellular necrosis were evident in the tap water + ethanol fed group 3. However, the active ceramic water treated group 1 showed normal architecture. Moreover, in group 2, mild fatty changes and necrosis were observed in hepatocytes. Collagen fibers were increased in spaces surrounding periportal and interlobular connective tissues in the group 3 of tap water + ethanol, but collagen synthesis and its thickness of fibrotic septa connecting portal tracts were markedly reduced in the group 2 of ceramic water + ethanol. Myofibroblasts were detected mainly in the interlobular connective tissues of pig liver of group 3 treated ethanol and tap water. Few to no myofibroblasts were observed in groups 1 and 2. CYP2E1 was not or rarely detected in group 1 fed ceramic water. However, group 2 showed slightly activation of CYP2E1 in the area of pericentral vein, while CYP2E1 was significantly activated in group 3 fed tap water and ethanol. Based on the above data, we believe that we have developed a unique alcohol induced fibrosis model in pig, which will be useful in developing anti-fibrotic agents and drugs. Furthermore, the active ceramic water used in our study had an inhibitory and may be protective against ethanol induced hepatic toxicity and fibrosis.

경주지역 소에서 분리한 탄저균에 관한 연구

김우현,정종식,서을원 안동대학교 기초과학연구소 1995 基礎科學 硏究論文集 Vol.6 No.1

1994년 2월 12일 경주시 백반동의 폐사한우에서 분리한 Bacillus anthracis로 여러가지 실험을 실시하여 다음과 같은 결과를 얻었다. 1. 생화학적 검사에서 그람양성, 아포와 협막을 형성하고, 탄저침강소 반응, 카타라제, V-P반응, 질산염 환원, 젤라틴 액화, 전분 가수분해, 혐기하에서 성장, 글루코스 분해, 카제인 가수분해, PH 5.7에서 성장, 7% NaCl 성장 등에서 양성이며, 운동성, 우레아제, 시트르산 이용, 인돌 산생, 아리비노즈,만니톨,키실로즈 분해, 45-65℃에서 성장 등에서는 음성이었다. 2. 각종 배지에서의 배양성은 24시간까지는 BA, Br A, TSA에 가장 좋았고, 72시간까지는 BA, BR A, NA, TSA에서 배양성이 좋았다. Colony배양성으로는 BA대용으로 Br A가 가장 좋았다. 3. 각종 동물 적혈구에 대한 β-용혈성시험은 대부분의 동물 적혈구가 36시간부터 용혈이 시작되어 48시간에 완전히 일어났으며, 산양 적혈구는 60시간부터 용혈이 시작되었다. 4. 항균제에 대한 감수성은 AM,CR,TE,ENR에 감수성이 가장 컷고, 그 다음은 GM,AN,DFX,S,P,TyLO,N,KM,C,E,Lins+SP,NN,CC,CFP,CB의 순이었으며, L,XNL,TIA에는 저항성을 나타내었고, CL,SXT에는 전혀 감수성이 없었다. 5. 탄저균을 피하접종하였을 때 rat와 chicken은 전혀 감수성이 없었고, mouse가 가장 감수성이 좋았고, 그 다음은 goat, hamster, guinea pig, rabbit의 순이 었다. 6. 탄저균 접종으로 폐사된 사체를 37℃ 부란기에 보존하였을 때 개복하지 않은 경우에는 80시간이후부터 탄저균이 검출되지 않았고, 개복한 상태에는 360시간이후에도 검출되었다. 7. 탄저균을 실험동물에 접종시 대체적으로 접종균수가 많을수록 빨리 폐사하였으며, 단지 420개의 피하주사에서도 폐사하였다. 8. 탄저균을 접종한 hamster의 혈액도말표본을 37℃에 보존시는 5시간, 32℃에서는 6시간, 실온에서는 9시간, -4℃와 -20℃에서는 10시간째에 아포를 형성하기 시작했다. 9. 탄저균을 실험동물의 피하 및 경구접종하였을 때 피하접종한 mouse, rat의 분변에서는 탄저균이 회수되지 않았고, 경구접종한 mouse,rat,chicken의 분변에서는 탄저균이 회수되었다. 10. 탄저균에 대한 가장 좋은 소독제는 formalin과 승흥이었으며, phenol과 cresol은 소독력이 약하였으며, NaOH는 소독력이 거의 없었고, ethanol은 전혀 소독력이 없었다. This study was conducted to investigate the characteristics of B, anthracis isolated from Anthrax in the Baeban Dong, Kyong-Ju in Feb. 12. 1994. 1. In biochemical features, B. anthracis showed a gram-positive rod, non-motility, sporulation and capsulation. It was positive in catalase, V-P reaction, nitrate reduction, gelatin liquefaction, starch hydrolysis, anaerobic growth, acid from glucose, casein hydrolysis, growth at PH 5.7, growth in 7% NaCl, but negative in urease, citrate utilization, indole production, acid from arabinose,manitol,xylose, growth at 45-65℃. 2. B. anthracis grew well on BA, Br A and TSA after incubation for 24 hours. The organism grew well on BA, Br A, NA and TSA after incubation for 72 hours. The good media for colony was Br A instead of BA. 3. On 5% blood agar by laboratory animal, β-hemolysis was produced from 36 hours to 48 hours incubation. There was perfect β-hymolysis after incubation for 48 hours. On the other side β- hemolysis was begun on 5% goat blood agar after incubation for 60 hours. 4. In the test of antimicrobial susceptibility, B. anthracis was very sensitive to AM, CF, TE and ENR. The orders of sensitivity were as follows: GM, AN, DFX, S,P, TYLO, N, KM, C, E, Lins+Sp, NN, CC, CFP and CB. B. anthracis was not sensitive to L, XNL, TIA, CL and SXT. 5. B. anthracis had no sensitivity to direct inoculation of rat and chicken, after subcutaneous injection. It was very sensitive to mouse. And the orders of sensitivity were as follows : goat, hamster, guinea pig and rabbit. 6. The dead laboratory animal which had been inoculated with B. anthracis preserved at 37℃ incubation, B. anthracis did not cultivate on non-dissected animal after 80 hours but cultivate on dissected animal after 360 hours. 7. The rapid death of animals could cause by high concentration of B. anthracis, even died from 420 after S.C. 8. In the blood smeared samples of hamster from inoculation with B. anthracis, the spore germinated in 37℃ after 5 hours, in 32℃ after 6 hours, in room temperature after 9 hours and in -4℃ to -20℃ after 10 hours. 9. B. anthracis inoculated to laboratory animal after S.C or P.O. mice and rats feces did not cultivated with B. anthracis after S.C, but cultivated with B. anthracis after P.O. 10. In the test of disinfectant, the most effective disinfectant was HgCl2 and formalin Phenol and cresol was less effective than HgCl2 and formalin. NaOH and ethanol was non-effective.

A pathological observation on the experimental rabbit viral hepatitis

정종식,권영란,이차수,신태균,Jyeong, Jong-sik,Kwon, Young-ran,Lee, Cha-soo,Shin, Tae-kyun The Korean Society of Veterinary Science 1992 大韓獸醫學會誌 Vol.32 No.4

This paper dealt with the clinical and pathological observations on the experimental rabbit viral hepatitis. Rabbits with 2-14 months of ages were intramuscularly inoculated with virus suspension. The results were summarized as follows. Ninety percents of experimental rabbits inoculated with virus died within 96 hours postinoculation. Common clinical signs were inappetence, increase in body temperature, depression, mild diarrhea and in three cases, bloody foam from nostrils was recognized. At necropsy, in most of the experimental cases, there were hyperemia or haemorrhages in many organs and pale liver. Intestinal catarrh and retention of turbid urine in urinary bladder were seen in some cases. Histopathologically, liver necrosis was found in all the cases died of this disease. However, there was a difference in the severity of hepatic necrosis. Haemorrhages were Iecognized in lungs, heart, liver, spleen, kidneys and thymus, in order. Liver necrosis was marked even in the cases with no haemorrhage. Perivascular cuffing in brain and catarrhal enteritis in small intestine were seen in many cases. From these results, consistent and primary lesion in this viral disease is hepatitis in susceptible rabbits. It was concluded that rabbit hepatitis virus might have the properties of hepatotropism and consequently induce peripheral necrosis in the liver leading to acute viremia with haemorrhages. 국내에서 발생된 토끼 virus성간염의 병리학적 소견을 명확히 규명하기 위하여 2~14개월령의 재래토끼에 간염에 이환된 토끼의 간조직 유제를 접종한후 임상 및 병리학적으로 관찰하였던 바 다음과 같은 결과를 얻었다. 접종한 30마리중 90%인 27마리가 감염되어 접종후 96시간이내에 폐사되었고 2~3월령 3두는 감염이 일어나지 않았다. 임상증상으로는 식욕부진, 체온상승, 연변 등이 나타났고 3두에서는 비강내로부터 혈액성 포말이 관찰되었다. 부검소견으로는 장기의 충출혈, 간장의 변성이 전예에서 관찰되었고 소장의 카타르성 장염과 방광내 혼탁한 뇨충만도 관찰되었다. 광학현미경적 소견으로는 간괴사가 정도의 차이가 있기는 하나 폐사된 27두 전예에서 관찰되었고, 출혈빈도는 폐가 가장 많았고 심장, 간장, 비장, 신장 및 흉선 순으로 나타났다. 한편 출혈이 관찰되지 않은 예에서도 간의 괴사소견은 현저하였다. 소장의 카타르성 장염과 뇌의 혈관주위 임파구 침윤도 관찰되었다. 이상의 결과를 종합해 볼 때 본 질병에서 출혈소견은 개체에 따라 변화가 심하였으나 중요한 소견은 간염으로 나타났고 원인 virus는 일차적으로 간에 친화성을 가지며 동시에 viremia를 일으켜 전신장기에 출혈을 야기하는 것으로 추정된다.

Further characterization of the causative virus of rabbit viral hepatitis, so-called rabbit haemorrhagic disease in Korea

정종식,정규식,이차수,신태균,Jyeong, Jong-sik,Jeong, Kyu-sik,Lee, Cha-soo,Shin, Tae-kyun The Korean Society of Veterinary Science 1992 大韓獸醫學會誌 Vol.32 No.3

국내에서 발생한 토끼 바이러스성 간염 소위 토끼 출혈병의 원인 바이러스를 감염토끼의 간조직으로 부터 분리정제한 후 바이러스의 핵산과 구성 단백질의 특징을 관찰하였던 바 다음과 같은 결과를 얻었다. 토끼간염바이러스는 분자량이 약 54 kilodalton인 한개의 구조단백을 가진 RNA 바이러스이며 바이러스 핵산의 크기는 약 7.5 kilobases로 나타났고 바이러스의 RNA는 배양세포에서는 감염을 일으키지 않았다. 바이러스 구성단백의 양상과 핵산의 크기 등을 종합해 볼 때 토끼의 간염 바이러스는 Caliciviridae에 속하는 것으로 간주된다. The causative virus causing rabbit hepatitis has been further characterized by evaluating viral proteins and viral nucleic acids of purified viruses from the liver of the experimentally infected rabbits. Rabbit hepatitis virus has one major structural protein of 54 kilodaltons and some minor proteins. Vrial RNA was resistant to DNAse I. The size of viral nucleic acid of this virus was calculated to be about 7.5 kilobases. These findings indicate that rabbit hepatitis virus belongs to the family Caliciviridae.

Alcohol-induced hepatic fibrosis in pig

( Chang Woo Lee ),( Jong Sik Jyeong ),( Cha Soo Lee ),( Kyu Shik Jeong ) 한국가축위생학회 2003 韓國家畜衛生學會誌 Vol.26 No.4

A number of toxicants have been incriminated as a causing hepatic disease. Among many detrimental injury, alcohol has been noted for hepatitis, fatty liver, fibrosis, and hepatic cirrhosis. The purpose of this study was to develop animal model for hepatic fibrosis in pigs fed ethanol, and to search for a new anti-fibrogenic agent via this model. Twelve male Landrace pigs were divided into 3 groups of 4 animals each. Group 1, 2 and 3 were fed with active ceramic water only, ceramic water + liquid diet containing 15% ethanol and normal tap water+liquid diet containing 15% ethanol for 12 weeks, respectively. At week 12, all pigs were immediately sacrificed for collection each tissue and blood. Serologically, serum ALT and AST levels were significantly reversed in group 2, as compared to group 3. They were normal range in pigs of group 1. Microscopically, macrovesicular lipid droplets and moderate hepatocellular necrosis were evident in the tap water+ethanol fed group 3. However, the active ceramic water treated group 1 showed normal architecture. Moreover, in group 2, mild fatty changes and necrosis were observed in hepatocytes. Collagen fibers were increased in spaces surrounding periportal and interlobular connective tissues in the group 3 of tap water+ethanol, but collagen synthesis and its thickness of fibrotic septa connecting portal tracts were markedily reduced in the group 2 of ceramic water+ethanol. Myofibroblasts were detected mainly in the interlobular connective tissues of pig liver of group 3 treated ethanol and tap water. Few to no myofibroblasts were observed in groups 1 and 2. CYP2E1 was not or rarely detected in group 1 fed ceramic water. However, group 2 showed slightly activation of CYP2E1 in the area of pericentral vein, while CYP2E1 was significantly activated in group 3 fed tap water and ethanol. Based on the above data, we believe that we have developed a unique alcohol induced fibrosis model in pig, which will be useful in developing anti-fibrotic agents and drugs. Furthermore, the active ceramic water used in our study had an inhibitory and may be protective against ethanol induced hepatic toxicity and fibrosis.

소의 이상산(異常産)에 대한 형청항체가 검사

김영환 ( Young Hwan Kim ),정종식 ( Jong Sik Jyeong ),원문부 ( Bunhu Hara ),죽하성일랑 ( Sei I ChroTake Sida ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.1

The cattle serum was investigated many cases of deformity calf in kyung-ju area. The serum antibody titer of Aino virus, which was not reported in domestic, was showed 3 cattle to ×2~64.

닭 Leucocytozoon병(病)의 집단발생(集團發生)과 병리학적(病理學的) 관찰(觀察)

곽수동,정종식,Kwak, Soo-Dong,Jyeong, Jong-Sik 대한수의학회 1983 大韓獸醫學會誌 Vol.23 No.2

The clinical and histopathological observation of leucocytozoonosis was carried out during July, 1982 at a poultry compound in Gyeongbuk province and the results were summarized as follows: 1. Leucocytozoonosis was observed in 36,900 of 59,900 chickens (61.6%) and the mortality rate was 18.3% (6,760 of 36,900 chickens infected). 2. Clinical findings were anorexia, dyspnea, paleness of comb, greenish diarrhea and reduction of egg production. 3. Gross lesions were yellowish white spots scattered on parenchymal organs and swelling of liver and spleen. In addition, petechiation and ecchymosis of small intestine, egg yolk and subcutaneous tissues were observed. 4. Histological findings were infiltration of mononuclear cells in liver, heart and lung, appearance of megaloschizonts in heart, liver, proventriculus and pancreas, proliferation of foreign body giant cells in heart, and hyperemia and swelling of all parenchymal organs.