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CRISPR/Cas9-mediated editing of PhMLO1 confers powdery mildew resistance in petunia
Xu Junping,Naing Aung Htay,강현희,Lee Su Young,Li Weilan,정미영,김창길 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.5
Mildew Locus O (MLO), which encodes a seven-transmembrane domain protein, is responsible for the development of powdery mildew disease symptoms in many plant species. Hence, we knocked out PhMLO1 from Petunia hybrida cv. Mirage Rose at the genomic level using the Clustered regularly interspaced short palindromic repeat/CRISPR-associated nuclease 9 (CRISPR/Cas9) system and investigated involvement of the gene in powdery mildew disease development in the petunia. The MLO1 transcript levels observed in all mlo1 mutants were significantly lower than that in the wild type (WT). Specifically, the transcript level was the lowest in the mutants (line no. 1, 25, 33, and 85), followed by the mutants (line no. 14, 17, and 81), and line no. 6. Disease development was positively linked to the higher transcript levels of PhMLO1, because the disease severity observed in WT was greater than that observed in the mutants (line no. 6,14, 17, and 81), and disease symptoms were almost not observed in the mutants (line no. 1, 25, 33, and 85). In addition, the transmission of the edited alleles from the T0 to T1 generation was also confirmed, and the disease severity results were identical to those observed in the T0 mutant lines. Overall, the editing of PhMLO1 completely or partially prevented the development of this disease in this petunia, depending on MLO1 transcript level of the mutants. This study highlights the role of PhMLO1 in the development of powdery mildew disease in petunia and points to the need to edit the MLO genes in other ornamental plants to improve ornamental quality against powdery mildew disease.
Junping Xu,Hye Ryun An,Pil Man Park,Yae Jin Kim,Su Young Lee 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
Phalaenopsis is an economically important horticultural crop as cut flowers and potted plants, and it has achieved commercial-scale production in many countries. Plant tissue culture technology is widely used in large-scale propagation of Phalaenopsis, which can not only maintain the stability of genotype and phenotype, but also effectively and rapidly reproduce on a large scale. In the present study, we tested the planting density (1, 4, 7, and 10 seedlings per bottle) of two commercial varieties P. ‘Lovely Angle’ and ‘Univivace’ in tissue culture production, and optimized the size (80mm, 110mm and 140mm in height), material (plastic and glass), and ventilation (with or without filter) of culture vessels for standard production. Our results showed that good ventilation was helpful to plant growth. ‘Lovely Angle’ has the highest rooting rate in plastic vessels compared with glass vessels, in contrast, ‘Univivace’ has 100% rooting rate in glass vessels. We also observed that the optimum height of the vessel should be 110mm with 7 ‒ 10 plants. This study could provide a basis for the standardized Phalaenopsis production for the P. ‘Lovely Angle’ and ‘Univivace’.
Xu, Feili,Wang, Junping,Guo, Yunchang,Fu, Ping,Zeng, Huawei,Li, Zhigang,Pei, Xiaoyan,Liu, Xiumei,Wang, Shuo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
This study firstly analyzed the antibiotic resistance, biochemical typing, and pulsed-field gel electrophoresis typing of 45 Bifidobacterium strains commonly used in health foods. Most strains were resistant to antibiotics but their antibiotic resistance rates were not high: Fos (56.52%), TET (43.48%), CRO (21.74%), AMC (15.22%), GEN (13.04%), RIF (10.87%), CHL (8.7%), CTX (6.52%), VAN (4.35%), and ERY (4.35%). The 45 strains could be divided into 14 pulsed-field gel electrophoresis types, of which the strain numbers of six pulsed-field gel electrophoresis types were more than one. All the Bifidobacterium strains could be divided into nine types by API50CHL biochemical identification. The same species displayed same biochemical typings, expect for B. animalis. Furthermore, the results confirmed that the same pulsed-field gel electrophoresis-type strains had closer antibiotic resistance patterns, and the same biochemical-type strain also had similar antibiotic resistance patterns.
Feili Xu,Junping Wang,Yunchang Guo,Huawei Zeng,Ping Fu,Zhigang Li,Xiaoyan Pei,Xiumei Liu,Shuo Wang 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
This study firstly analyzed the antibiotic resistance, biochemical typing, and pulsed-field gel electrophoresis typing of 45 Bifidobacterium strains commonly used in health foods. Most strains were resistant to antibiotics but their antibiotic resistance rates were not high: Fos (56.52%), TET (43.48%), CRO (21.74%), AMC (15.22%), GEN (13.04%), RIF (10.87%), CHL (8.7%), CTX (6.52%), VAN (4.35%), and ERY (4.35%). The 45 strains could be divided into 14 pulsed-field gel electrophoresis types, of which the strain numbers of six pulsed-field gel electrophoresis types were more than one. All the Bifidobacterium strains could be divided into nine types by API50CHL biochemical identification. The same species displayed same biochemical typings, expect for B. animalis. Furthermore, the results confirmed that the same pulsed-field gel electrophoresis-type strains had closer antibiotic resistance patterns, and the same biochemicaltype strain also had similar antibiotic resistance patterns.