Bifidobacteria에 의한 우유발효와 유산박테리아를 이용한 Bifidobacterium bifidum ATCC 11863의 이용성증진에 관한 연구

고준수,윤영호,권일경,안종건 한국낙농학회 1987 韓國酪農學會誌 Vol.9 No.4

本 硏究는 B. bifium을 利用한 醱酵 유제품 제조를 위한 기초연구로서 B. bifidum을 포함한 bifidobacteria의 醱生成能力 및 L-cysteine·HCI과 yeast extract의 첨가효과를 비교하였고, B. bifidum과 유산박테리아의 混合培養의 가능성을 규명하였다. 1. B. bifidum ATCC 11863과 B. breve는 還元脫脂乳培地에서 B. infantis와 B. adolescentis 보다 酸生成이 우수하여 bifidobacteria 種間에 차이를 나타내었다. 2. L-cysteine과 yeast extract를 환원탈지유에 첨가했을 때 bifidobacteria의 酸生成을 增進하였다. 3. B. bifidum ATCC 11863을 원유에서 醱酵할 때 L. acidophius, L. casei 및 S. thermophilus보다 酸生成 및 성장이 현저하게 낮았다. 4. B. bifidum과 乳酸박테리아를 牛乳에서 混合醱酵할 때 酸生成이 매우 增進되었으며, B. bifidum ATCC 11863과 L. casei를 혼합발효할 때 酸生成이 제일 좋았다. Acid production in reconstituted skim milk by B, bifidum ATCC 11863 was compared with that of other bacteria related to the effect of L-cysteine·HCl and yeast extract. And mixed starter cultures of B. bifidum ATC11863 and lactic acid bacteria were tried to enhance the acid production in whole milk. The results are as follows: 1. The acid production of B. bifidum ATCC 11863 in reconstituted skim milk was similar to that by B. breve, and it was better than that by B. infantis or by B. adolescentis. 2. The addition of L-cysteine·HCl and yeast extract to reconstituted skim milk enhanced acid production by bifidobacteria. 3. Compared to those by L. acidophilus, L. casei and S. thermophilus, B. bifidum ATCC 11863 evidenced a delayed growth and slower acid production in whole milk. 4. B. bifidum ATCC 11863 produced more acid when grown with L. casei, L. acidophilus or S. thermophilus than that by pure culture. The acid production was best when B. bifidum ATCC 11863 was used with L. casei.

Lactobacillus acidophilus 와 Streptococcus thermophilus 에 의한 Ultrafiltration 처리우유의 발효 촉진 : II. 발효 및 저장중의 유성분 변화 II. Conversion of Milk Constituents during Fermentation and Storage

고준수,안종건,박재인,권일경,김거유 한국낙농학회 1992 韓國酪農學會誌 Vol.14 No.2

Lactobacillus acidophilus와 Streptococcus thermophilus를 사용하여 UF-retentate, 전유, 농축유를 발효하였을때 가용성질소화합물, 아미노산, 탄수화물 함량의 변화와 저장 중 산 및 젖산균 변화를 무기물(FeSO₄, MgSO₄, MnCL₂)과 L-cysteine의 영향과 함께 조사하였다. UF-Retentate와 농축유가 원료유일 경우 L. acidophilus와 S. thermophilus를 함께 사용한 복합균주로 발효했을 경우와 L. acidophilus 단독균주로 발효했을 경우 모두 가용성 질소함량의 증가는 매우 적었고, 전유의 경우 L. acidophilus 단독균주를 사용했을 때에만 가용성질소가 현저하게 증가하였다. L-Cysteine는 이와같은 양상에 영향을 미치지 않았고, UF-retentate와 농축유에 무기물을 첨가했을때 L. acidophilus단독균주에 의한 가용성질소의 생성량을 증가시킬 수 있었다. 발효후에 유당함량은 현저하게 감소했으며 L-cysteine은 유당함량 변화에 영향을 주지 않았으나 무기물은 UF-retentate를 L. acidophilus 단독균주로 발효한 경우와 전유를 L. acidophilus와 S. thermophilus 복합균주로 발효 시킨 경우를 제외하고는 유당함량의 감소를 촉진하였다. 발효후 대부분의 아미노산함량이 증가했으며 특히 glutamic acid의 함량이 많이 증가하였다. L-Cysteine은 L. acidophilus 와 S. thermophilus복합균주에 의해 제조된 발효유의 저장중 산 및 생존젖산균수의 변화에 영향을 미치지 않았다. This research was performed to study the effect of minerals(FeSO₄, MgSO₄, MnCl₂)and L-cysteine on the change of constituents of fermented ultrafiltration retentate(UF-retentate), whole milk and evaporated milk during fermentation by Lactobacillus acidophilus and Streptococcus thermophilus, and during stroage at 9℃ for 96 hours. The soluble nitrogen content increased a little in UF-retentate and evaporated milk fermented by mixed culture(L. acidophilus and S. thermophilus) and single culture(L. acidophilus), but whole milk fermented by single culture showed high increase in the soluble nitrogen value. L-Cysteine did not show any effect on the change of the soluble nitrogen content during fermentation, but minerals increased the soluble nitrogen content very much in UF-retentate and evaporated milk fermented by single culture during fermentation. Lactose content was decreased significantly by fermentation. L-Cysteine had no effect on the change of lactose content, and minerals stimulated the decrease of lactose content in fermented milks except UF-retentate fermented by single culture and whole milk fermented by mixed culture during fermentation. Fermentation increased the content of almost of amino acids especially that of glutamic acid. L-Cysteine added before fermentation had no effect on the change of acidity and number of viable lactic acid bacteria in fermented milks during storage at 9℃ for 96 hours.

Lactobacillus acidophilus 와 Streptococcus thermophilus 에 의한 Ultrafiltration 처리우유의 발효 촉진 : I. 무기물과 L - cysteine 이 산생성 및 유산균 변화에 미치는 영향 I. Effect of Minerals and L - cysteine on Acid Production and Viable Lactic Acid Bacteria in Fermented Milk

고준수,김거용,제등선일 한국낙농학회 1990 韓國酪農學會誌 Vol.12 No.2

Lactobacillus acidophilus와 Streptococcus thermophilus에 의한 ultrafiltration處理 牛乳의 醱酵促進을 위해 利用된 無機物과 L-cysteine의 효과를 要約하면 다음과 같다. 1. L. acidophilus 單獨 培養時 酸生成은 全乳, UF-retentate 및 濃縮乳중 全乳에서 가장 높게 나타났으며, L. acidophilus와 S. thermophilus를 混合 培養할때의 젖산 濃度 증가는 UF-retentate에서 제일 높게 나타났다. 생존 젖산 균수는 L. acidophilus 단독 培養時에는 거의 유사하나, 混合 培養時에는 UF-retentate에서 제일 많았다. 2. L. acidophilus와 S. thermophilus 混合 培養時의 酸生成이 L. acidophilus 單獨培養時보다 더 높은 수준을 나타내었다. 생존 젖산 균수에 있어서는 L. acidophilus 單獨 培養時 더 높았으나 UF-retentate에서는 單獨 및 混合 培養時 生存 젖산 菌數가 유사하였다. 3. L. acidophilus 單獨 培養時 全乳, UF-retentate 및 濃縮乳에 無機物을 添加하면 酸生成이 促進되며, 微生物 數가 증가하였고, L. acidophilus와 S. thermophilus를 複合 培養시에는 濃縮乳에서만 徵生物 數가 증가하였다. 4. L. acidophilus 單獨 培養時 UF-retentate에 L-cysteine을 添加했을때 酸生成이 促進된다. The effects of minerals and L-cysteine on the fermentation of ultrafiltrated milk by Lactobacillus acidophilus and Streptococcus thermophilus are summerized as follows; 1. L. acidophilus inoculated solely produced more acid in whole milk than in UF-retentate and evaporated milk, mixed culture of L. acidophilus and S. thermophilus produced acid most in UF-retentate. The viable cells of lactic acid bacteria of the sole culture of L. acidophilus were similar in whole milk, UF-retentate and evaporated milk, whereas viable cells of the mixed cultures of L. acidophilus and S. thermophilus were highest in UF-retentate. 2. The mixed culture of L. acidophilus and S. thermophilus produced more acid than the sole culture of L. acidophilus. The viable cells of L. acidophilus inoculated independently usually showed the higher level, but those of the mixed and the sole cultures were similar in UFretentate. 3. Minerals added to whole milk, UF-retentate and evaporated milk, increased the acid production and viable cells of L. acidophilus. In the case of fermentation by the mixed culture of L. acidophilus and S. thermophilus, minerals only increased the viable cells in evaporated milk. 4. L-cysteine stimulated acid production in UF-retentate formented by the sole culture of L. acidophilus.

유지방구로부터 분리한 Lipase 의 안정성과 활성에 미치는 Casein 의 영향

고준수,권일경,강창기,김거유 한국낙농학회 1994 韓國酪農學會誌 Vol.16 No.2

유지방구로 부터 분리 정제한 lipase의 안정성 및 활성에 미치는 케이신의 영향에 대하여 검토 하였으며 그 결과는 다음과 같다. 1. α_(s-), β-, κ- 케이신은 정제 lipase의 활성저하에 대한 보호효과를 나타내었다. 2. 효소와 기질만의 단순한 반응조건에서는 케이신의 첨가에 의하여 활성이 증가 하였으며 활성화 효과는 반응온도 0℃ 보다 37℃에서 높은 결과를 나타내었다. 3. 1 % BSA를 기질에 첨가하였을 경우, 각 케이신에 의한 활성의 변화는 없었으며, 0.1% BSA를 첨가하였을 경우에는 다시 활성이 증가하였다. 반응온도 37℃의 경우 β-케이신의 첨가에 의한 활성화 효과는 나타나지 않았다. The effects of casein on the stability and activity of the lipase from milk fat globules were investigated. 1. The protective effect of α_(s-), β-, κ-casein against inactivation of purified lipase was significant. 2. In the case of simple substrate system, i. e., absence of added blood serum albumin(BSA), the addition of casein increased the activity of lipase. Activation effect of the casein was more higher at 37℃ than at 0℃. 3. The lipase activity was not changed when coconut oil containing of 1% BSA was used instead of coconut oil as substrate. But in the presense of 0.1% BSA, casein fractions except β-casein at 37℃ increased the lipase activity.

Poster Exhibition : High Expression of Microrna-15b Predicts a Low Risk of Tumor Recurrence Following Curative Resection of Hepatocellular Carcinoma (초)

Goh Eun Chung,Jung Hwan Yoon,Sun Jung Myung,Jeong Hoon Lee,Sung Hee Lee,Soo Mi Lee,Seung Jun Kim,Seung Yong Hwang,Hyo Suk Lee,Chung Yong Kim 대한간학회 2009 Clinical and Molecular Hepatology(대한간학회지) Vol.15 No.4(S)

원유의 지방분해에 미치는 온도활성화 및 교반의 영향

고준수,권일경,김거유 한국낙농학회 1995 韓國酪農學會誌 Vol.17 No.4

High expression of microRNA-15b predicts a low risk of tumor recurrence following curative resection of hepatocellular carcinoma.

Chung, Goh Eun,Yoon, Jung-Hwan,Myung, Sun Jung,Lee, Jeong-Hoon,Lee, Sung-Hee,Lee, Soo-Mi,Kim, Seung-Jun,Hwang, Seung Yong,Lee, Hyo-Suk,Kim, Chung Yong National Hellenic Research Foundation 2010 ONCOLOGY REPORTS Vol.23 No.1

<P>MicroRNAs (miRNA) have recently been implicated in carcinogenesis and tumor progression. Hepatocellular carcinoma (HCC) is well known for frequent relapses following curative resection. We attempted to identify the miRNAs associated with HCC recurrence. We analyzed miRNA expression profiles in 25 pairs of HCC and adjacent non-tumor liver tissues from HCC patients using miRNA microarray. Out of 449 miRNAs assayed, we identified seven miRNAs associated with HCC recurrence. In particular, the highest ranked miR-15b was negatively correlated with recurrence. MiR-15b inhibitor transfection increased HCC cell proliferation and inhibited TRAIL-induced apoptosis, while miR-15b precursor transfection decreased proliferation and enhanced apoptosis. Bcl-w was identified as a target molecule regulated by miR-15b. These results indicate that miR-15b expression in HCC tissues may predict a low risk of HCC recurrence. In addition, the modulation of miR-15b expression may be useful as an apoptosis-sensitizing strategy for HCC treatment.</P>

강박장애환자의 Rey-Osterrieth Complex Figure Test에서의 정보처리유형과 회상율에 관한 연구

박수진 ( Soo Jin Park ),이영호 ( Young Ho Lee ),권준수 ( Jun Soo Kwon ),김창윤 ( Chang Yoon Kim ),고진경 ( Jin Kyung Goh ),하태현 ( Tae Hyon Ha ) 한국정신병리진단분류학회 2003 精神病理學 Vol.12 No.1

CSS/ToA 기반 RTLS의 현장 적용 시험

고형준 ( Hyung-jun Goh ),류대현 ( Dae-hyun Ryu ),두주영 ( Ju-young Du ),김연수 ( Yeon-soo Kim ) 한국정보처리학회 2008 한국정보처리학회 학술대회논문집 Vol.15 No.2

유비쿼터스 환경에서 ‘상황인식 서비스(context-aware service)’를 구현하기 위해서는 위치 정보 및 이를 기반으로 한 주변의 상황에 대한 정보, 그리고 거기에 적합한 서비스 제공 인프라를 구축하는 것이 필요하다. 본 연구에서는 최근 IEEE 802.15.4/a에서 표준화가 완료된 CSS(Chirp Spread Spectrum) 기반의 RTLS를 구축하고 성능을 평가한다.

Treatment Efficacy of Endoscopic Variceal Obturation for Gastric Variceal Bleeding According to the Type of Varices

( Hyun Gil Goh ),( Yeon Seok Seo ),( Han Ah Lee ),( Tae Hyung Kim ),( Sang Jun Suh ),( Young Kul Jung ),( Ji Hoon Kim ),( Hyunggin An ),( Hyung Joon Yim ),( Jong Eun Yeon ),( Kwan Soo Byun ),( Soon Ho 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: This study was performed to evaluate the treatment efficacy of endoscopic variceal obturation (EVO) in patients with gastric variceal (GV) bleeding according to the type of varices. Methods: All patients who treated with EVO for GV bleeding were included. Patients with previous history of endoscopic treatment for GV bleeding (n=4) and those combined with portal vein invasion by HCC (n=9) or other malignancy (n=7) were excluded. In addition, patients with IGV2 were also excluded because the number was too small for analysis (n=2). Results: Age was 59.4 ± 12.4 years and 72 patients (79.1%) were men. Alcoholic liver disease (47 patients, 51.6%) was most common cause of liver disease, followed by chronic hepatitis B (24 patients, 26.4%). HCC was combined in 28 patients (30.8%). Type of varices was GOV1, GOV2, and IGV1 in 33 (36.3%), 31 (34.1%), and 27 (29.7%) patients, respectively. Hemostasis was successful in 88 patients (96.7%). Hemostasis success rates according to the type of varices were 100%, 96.8%, and 92.6% in GOV1, GOV2, and IGV1, respectively, which were comparable among three groups (P=0.279). Hemostasis was successful in 88 patients (96.7%). GV was obliterated in 81 patients (89.0%). Number of EVO session was comparable among three groups (1.5 ± 0.7, 1.3 ± 0.4, and 1.7 ± 0.7 in patients with GOV1, GOV2, and IGV1, respectively), while amount of histoacryl was significantly smaller in patients with GOV1 (1.4 ± 0.7 ml) compared to those with GOV2 (2.6 ± 1.3 ml, P=0.042) and IGV1 (2.7 ± 1.4 ml, P=0.041). Among 81 patients with GV obliteration, GV recurrence rate at 24 months was 43.9%. GV recurrence was significantly lower in patients with GOV1 (18.7%) than those with GOV2 (65.0%; P=0.005) and those with IGV1 (47.0%; P=0.046). VB recurrence rate at 24 months was 21.3%. VB recurrence rate was significantly lower in patients with GOV1 (12.1%) than those with GOV2 (40.5%; P=0.021), but it was comparable with patients with IGV1 (11.6%; P-0.929). Conclusions: Size of varices and amount of histoacryl for GV obliteration were significantly lower in patients with GOV1 than those with GOV2 or IGV1. GV recurrence, GV rebleeding were significantly lower in patients with GOV1 than those with GOV2.