The novel herbal cocktail MA128 suppresses tumor growth and the metastatic potential of highly malignant tumor cells

KIM, AEYUNG,IM, MINJU,YIM, NAM-HIU,HWANG, YOUN-HWAN,YANG, HYE JIN,MA, JIN YEUL Spandidos Publications 2015 ONCOLOGY REPORTS Vol.34 No.2

<P>MA128, a novel herbal medicine, was previously identified and its effectiveness in the treatment of asthma and atopic dermatitis (AD) was demonstrated. In particular, post-inflammatory hyperpigmentation (PIH) in AD mice was improved by treatment with MA128. In addition, MA128 exhibited anti-melanogenic activity by inhibiting tyrosinase activity via the p38 MAPK and protein kinase A signaling pathways in B16F10 cells. In the present study, we examined whether oral administration of MA128 suppressed the in vivo tumor growth of HT1080 cells in athymic nude mice. The results showed that the daily oral administration of 75 and 150 mg/kg MA128 suppressed the tumorigenic growth of HT1080 cells efficiently. Since metastasis is a major cause of cancer-associated mortality and the greatest challenge during cancer treatment, we investigated the effect of non-toxic concentrations of MA128 on the metastatic potential of HT1080 cells. MA128 inhibited anchorage-independent colony formation, migration and invasion. Matrix metalloproteinase-9 (MMP-9) activity under resting and PMA-stimulated conditions was decreased in a dose-dependent manner by MA128 in HT1080 cells. In addition, the daily oral administration of MA128 at doses of 75 and 150 mg/kg efficiently blocked the lung metastasis of B16F10 cells that had been injected into the tail veins of C57BL/6 mice. In particular, none of the mice treated with MA128 exhibited systemic toxicity, such as body weight loss or liver and kidney dysfunction. MA128 also inhibited tumor-induced angiogenesis. Taken together, the results suggest that MA128 is a potential therapeutic agent and a safe herbal medicine for controlling malignant and metastatic cancer.</P>

Anti-melanogenic activity of the novel herbal medicine, MA128, through inhibition of tyrosinase activity mediated by the p38 mitogen-activated protein kinases and protein kinase signaling pathway in B16F10 cells

Kim, Aeyung,Ma, Jin Yeul Medknow PublicationsMedia Pvt Ltd 2014 Pharmacognosy magazine Vol.10 No.39

<P><B>Background:</B></P><P>Recently, our research group developed MA128, a novel herbal medicine, and demonstrated that MA128 is effective for the treatment of asthma and atopic dermatitis (AD). In particular, postinflammatory hyper-pigmentation in AD mice was improved with MA128 treatment. Thus, in this study, we determined the effect of MA128 on melanogenesis and its underlying mechanism in murine B16F10 melanoma cells.</P><P><B>Materials and Methods:</B></P><P>After treatment with MA128 at 100 and 250 μg/mL and/or alpha-melanocyte stimulating hormone (α-MSH) (1 μM), cellular melanin content and tyrosinase activity in B16F10 cells were measured. Using western blotting, expression levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, microphthalmia-associated transcription factor (MITF), and activation of c-AMP-dependent protein kinase (PKA), c-AMP-related element binding protein (CREB) and mitogen-activated protein kinases (MAPKs) were examined.</P><P><B>Results:</B></P><P>MA128 significantly inhibited melanin synthesis and tyrosinase activity in a resting state as well as α-MSH-stimulating condition, and significantly decreased the expression of tyrosinase, TRP-1, TRP-2 and MITF. In addition, phosphorylation of PKA and CREB by α-MSH stimulation was efficiently blocked by MA128 pretreatment. Moreover, MA128 as an herbal mixture showed synergistic anti-melanogenic effects compared with each single constituent herb.</P><P><B>Conclusion:</B></P><P>MA128 showed anti-melanogenic activity through inhibition of tyrosinase activity mediated by p38 MAPK and PKA signaling pathways in B16F10 cells. These results suggest that MA128 may be useful as an herbal medicine for controlling hyper-pigmentation and as a skin-whitening agent.</P>

Oral Administration of Novel Oriental Medicine, KIOM-C, Protect against Influenza Virus

Jin Yeul Ma, Eun Ha Kim, Jun Han Lee, Min-Suk Song, Yun Hee Baek, Young Ki Choi 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2

The influenza virus is an important respiratory risk affecting humans, and effective treatments are needed. Some oriental medicines are currently applied for treatment of common colds as well as influenza infection. Previous studies have reported that the therapeutic properties of MA-128 are effective for treatment of psoriasis antiasthmatic and atopic dermatitis. In this study, we investigated the therapeutic properties of the novel herbal medicine, MA-128, for treatment of influenza virus infection by oral administration. MA-128 is an active natural biological compound from herbal-marine origin. The results showed that oral administration of MA-128 in mice could confer a survival benefit against Type A influenza virus infection. Daily oral administration of MA- 128 resulted in delayed death in infected mice for three days against mouse adapted H3N2 (A/Philippines/2/82). However, it protected more than 60% of mice from lethal infection of 2009 pandemic H1N1 (A/Korea/CJ01/2009) influenza virus. In addition, lung viral titers were significantly reduced at seven days post infection (~100 times) compared with mock-treated mice and viruses were cleared at 9 dpi only in the MA-128 treated groups. This study demonstrated the potential of the novel herbal medicine, MA-128, as an herbal remedy against influenza A viruses.

EC地域에 대한 投資家能性과 展望

馬鎭浩,崔炳龍,孫炳海,李東萬 慶北大學校 經商大學 經濟學科 1979 慶商大學論集 Vol.7 No.-

海外直接投資가 貿易에 미치는 效果

馬鎭浩 慶北大學校 經商大學 經濟學科 1983 慶商大學論集 Vol.11 No.-

低開發國의 工業化過程에 關한 硏究

馬鎭浩 慶北大學校 1976 論文集 Vol.22 No.-

There have been many differences in understanding the true meaning of industrialization, and how the process of economic development in underdeveloped countries should be. Korea, since starting the first five-Year Economic Development Plan in 1962, was rapidly developed; its average annual rate of growth was over 9 percent in this decade, and the ratio of the contribution of the manufacturing and mining sector to the GNP rose from 16.5 percent in 1962 to 29.0 percent in 1974, and the export expansion was dramatically increased over 40 percent at an average annual rate through those years. However, its dependency on the foreign sector is also increasing rapidly; that is, its foreign trade/GNP ratio continued to rise from 24.7 percent on the average through the First Five-Year Plan to 75.3 percent in 1974. It is well known that such an economy heavily depending on the foreign sector could be damaged severly if the world economy should slump into a depression. This paper attempts to inquire the true meaning of industrialization and the demerits of expart-biased industrialization, and also to study how the process of industrialization in underdeveloped countries should be. The results of the study are summarized as follows: 1. the true meaning of industrialization lies on advancing the labor productivity of the economy. 2. In early stages of industrialization in underdeveloped countries it is inevitable that there is a bias towards exportable consumer goods because they need to get more foreign currency to purchase capital goods from advanced countries, and also the consumer goods industry would easily become a comparative advantage industry due to its labor intensity, 3. In the next stage, however, the policy aim should be converted into promoting the import substition of durable goods for avoiding the difficulties of international balance of payments, increasing the carry-over effects, and also advancing its labor productivity. 4. If these processes of industrialization in underdeveloped countries were accomplished successfuly, and the dependeney on foreign sector decreased sufficiently, the country could get the power of self-sustained growth, and step into the third stage competing with the other advanced countries.

貿易構造 決定原理에 관한 硏究

馬鎭浩 慶北大學校 經商大學 經濟學科 1973 慶商大學論集 Vol.1 No.-

Vibrio parahemolyticus에 대한 한방처방 [方藥合編] 및 그 단미제의 항균활성에 관한 연구

마진열,김진숙,신순식,정규용,박갑주 한국한의학연구원 1999 한국한의학연구원논문집 Vol.5 No.1

Vibrio are become prevailing if superficial temperature of ocean is raised and their activities of area are expanded and most of ocean creatures (fishes, oysters etc) are polluted with vibrio. The one who has taken these polluted fishes and aysters uncooked caused foodpoisoning and diarrhea from Vibrio. Frequencise of these diseases breakout is disposed in westsea shore of Korea. According to ancient and traditional Korean medical book -『Bangyak Happyeon』(Collection of Local Medicines, 1884) - and their single prescibes, we carried out experiment check the activities of natural medicinal effects on Vibrio parahemolyticus. The prescriptions of trial materials are processed from extraction boiling water and 80% methanol and followed freeze dried and adsorbed to every discs in dosage of 10mg. Gentamycin of 10mg were used for control. The result of compound prescription displayed special diseases in antimicrobial activities of boiling water and MeOH extraction compared with control. In compound prescription, extraction MeOH of Sashinhwan(clear zone : 17mm) presented extraordinaire antimicrobial activity. In single prescription, extraction of boiling water (clear zone : 16mm) and MeOH(clear zone : 18mm) of Fructus Chebulae presented extraordinaire antimicrobial activity. The MBC of Fructus Chebulae extracts was expressed in boiling water (1.28mg/ml) and MeOH(0.64mg/ml).

龜尾地域의 工業化에 따른 諸問題點과 그 對策에 關한 硏究 : 經濟的 側面 an economic perspective

馬鎭浩 慶北大學校 東洋文化硏究所 1977 東洋文化硏究 Vol.4 No.-

This study explores and analyze some problems in the process of industrialization of Gumi area and then recommends some preparatory measures to cope with these circumstances. Chapter One present an introductory role to understanding the Gumi industrial complex by tracing its history, location and present conditions. Chapter Two explores the socio-economic effects brought about by industrialization of Gumi Area in three parts: (1) the effects on employment; (2) the effects on income growth; (3) the effects on export increase. The effects on employment enlargement goes to the extent that the industrialization of the Gumi area has greatly reduced the disguised unemplaymenis which had been existed in this once rural area; especially the reduction is remarkable for the unmarried female labor force who are the main man-power of the industrial complex. Not only those unmarried female laborers in this community but also those in the neighboring communities are diffused to be employed. At present it is estimated that this portion of labor force are almost fully employed. In the second place, the industrialization of the Gumi area has made a great contribution to the income growth of the people of this area. Despite the existing factors that might cause to decrease the income level such as the erosion of arable land, the increase of tax burden, and the rise of prices of commodity, it is estimated that the industrialization of the Gumi area has brought increase in income of the people of the area due to the increase of the employment, increase in property income owing to the rise of property prices and prosperity in various kinds of service industry. Therefore, factors leading to the increase of income overwhelm those leading to the opposite. Thus, the net increase of income per year is estimated to reach about five billions won in total which means 200,000 won of yearly income per household of this area. Thirdly, goal achievement has not been satisfactory in the area of export increment effect. Despite its purpose as an export industrial complex, only one third-of its total products has been exported. Out of 100 operating companies, twentyfour companies have never exported their production to foreign countries. Chapter Three analytically discusses various problems caused by industrilaizing Gumi area in terms of three perspectives: (1) efficiency aspect of the Gumi industrial complex management; (2) the problems of supporting projects (3) supply and demand of labor forces. The chapter also presents some measures to cope with these problems. First, in the discussion of the efficiency of the industrial complex management, several points including inefficiency of land use, unsatisfactory condition of export, lack of cooperation and production line arrangement between firms have been discussed with the proposal of some recommendations to remedy these conditions and problems. Secondly, problems pointed toward the supporting projects include the shortage of transportation instruments, housing and others. These problems are seriously discussed with the proposal of recommendations to be implemented both in short term and long term. Thirdly, the discussion of the problems of supply and demand in labor force analyzes and explores the prospective demand of labor force and the possibility of supply of labor force. It is expected that the near future will see shortage of labor force. Some measures to meet these problems have been proposed both on a short term and long term basis. Lastly in the concluding chapter, we have discussed some difficulties found in the process of industrialization of the Gumi area and summarizes our recommendations, We have conclusively made it clear that achieving a smooth progress in the industrialization of this area, most of all, requires formation of background cities surrounding the Gumi area.

美國의 學問硏究動向 調査硏究 : 經濟學과 Artaud 의 演劇을 中心으로 In the Fields of Economics and Artaud's Theatre

馬鎭浩,朴奉斗,許榮 경북대학교 1981 새마을 硏究論叢 Vol.1 No.-