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Minghao Zhang,Dan Cai,Qiumei Song,Yu Wang,Haiyue Sun,Chunhong Piao,Hansong Yu,Junmei Liu,Jingsheng Liu,Yuhua Wang 한국축산식품학회 2019 한국축산식품학회지 Vol.39 No.3
Lactobacillus rhamnosus GG (LGG) has low resistance to low pH and bile salt in the gastrointestinal juice. In this study, the gel made from whey protein concentrate (WPC) and pullulan (PUL) was used as the wall material to prepare the microencapsulation for LGG protection. The gelation process was optimized and the properties of gel were also determined. The results showed the optimal gel was made from 10% WPC and 8.0% PUL at pH 7.5, which could get the best protective effect; the viable counts of LGG were 6.61 Log CFU/g after exposure to simulated gastric juice (SGJ) and 9.40 Log CFU/g to simulated intestinal juice (SIJ) for 4 h. Sodium dodecyl sulphite polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that the WPC-PUL gel had low solubility in SGJ, but dissolved well in SIJ, which suggested that the gel can protect LGG under SGJ condition and release probiotics in the SIJ. Moreover, when the gel has highest hardness and water-holding capacity, the viable counts of LGG were not the best, suggesting the relationship between the protection and the properties of the gel was non-linear.
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Structural and magnetic properties of Si1−xGex thin films implanted with Fe ions
Li Wang,Deng-Lu Hou,Jingsheng Yu,Wengang Wei,Cong-Mian Zhen,Li Ma,Fengchun Hu,Chao Wang 한국물리학회 2013 Current Applied Physics Vol.13 No.4
A series of Si1-xGex (x = 1, 0.848, 0.591, 0.382, 0.209, 0.064, 0) thin films prepared by ion beam sputtering were implanted with Fe ions to different doses using the metal vapor vacuum arc technique. X-ray absorption fine structure (XAFS) was used to characterize the local microstructure around the Fe atoms in Fe-doped Si1-xGex samples. Structural analysis showed that for annealed samples of Ge-rich thin films (including pure Ge) implanted with low doses of Fe ions, almost all the Fe ions substituted at Ge sites. However, an anti-ferromagnetic Fe6Ge5 impurity phase existed in the annealed samples implanted with high doses of Fe. It was also found that the solubility of Fe ions was highest in pure Ge films and that with increasing Si concentration, the solubility decreased. Magnetic analysis showed that for the as-implanted and annealed samples of Ge-rich thin films implanted with Fe ions, room-temperature ferromagnetism was strongest in the pure Ge series of samples and that as the Ge concentration decreased, the ferromagnetism at room temperature weakened. In addition, annealing could increase the number of Fe ions at substitution sites, which resulted in the observed increase in the saturated magnetization after annealing. Experiment and theoretical analysis showed that the ferromagnetism of Fe-doped Ge-rich Si1xGex thin films samples originated from the s, ped exchange interactions between the Si1-xGex matrix and those Fe ions which substituted at Ge sites and that the ferromagnetism was mediated by carriers.
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Production of Transgenic Goats by Sperm-mediated Exogenous DNA Transfer Method
Zhao, Yongju,Wei, Hong,Wang, Yong,Wang, Lingbin,Yu, Mingju,Fan, Jingsheng,Zheng, Shuangyan,Zhao, Cong Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.1
In this study, the production of transgenic goats using sperm to integrate exogenous DNA and artificial insemination (AI) was carried out and the technical protocols for sperm-mediated gene transfer (SMGT) in the goat were optimized. The standard sperm parameters and the ability to bind foreign genes were assessed to select suitable sperm donor bucks. A total of 134 oestrous does were divided into 4 groups and inseminated using different methods and sperm numbers. The does of Groups I to III were inseminated with fresh semen ($1-2\times10^{7}$ and $10^{6}$ sperm) or frozen-thawed semen ($10^{6}$ sperm), respectively, through conventional intra-cervical AI, and the does of Group IV with frozen-thawed semen ($10^{6}$ sperm) through intrauterine AI. Total genomic DNAs were extracted from ear biopsies of the offspring. The presence of $pEGFP-N_{1}$ DNA was screened by PCR and then by Southern blotting analysis. A total of 76 live kids were produced and 8 kids were tested transgene positive on the basis of agarose gel electrophoresis of the PCR-amplified fragment. Southern blotting analysis of the samples showed 5 positive kids. A transgenic ratio of 10.53% was detected using PCR and 6.58% using Southern blotting. The positive kid rate assayed by PCR and Southern blotting of frozen-thawed goat semen was 3.61% and 9.27% higher than that of untreated semen. The results show that transgenic goats can be produced efficiently by the method of artificial insemination using sperm cells to integrate the exogenous DNA and intrauterine insemination allowed low numbers of DNA-transfected spermatozoa to be used, with satisfactory fertility.
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Ginsenoside Rk1 inhibits HeLa cell proliferation through an endoplasmic reticulum signaling pathway
Qiuyang Li,Hang Sun,Shiwei Liu,Jinxin Tang,Shengnan Liu,Pei Yin,Qianwen Mi,Jingsheng Liu,Lei yu,Yunfeng Bi 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.5
Background: Changes to work-life balance has increased the incidence of cervical cancer among youngerpeople. A minor ginseng saponin known as ginsenoside Rk1 can inhibit the growth and survival ofhuman cancer cells; however, whether ginsenoside Rk1 inhibits HeLa cell proliferation is unknown. Methods and results: Ginsenoside Rk1 blocked HeLa cells in the G0/G1 phase in a dose-dependentmanner and inhibited cell division and proliferation. Ginsenoside Rk1 markedly also activated theapoptotic signaling pathway via caspase 3, PARP, and caspase 6. In addition, ginsenoside Rk1 increasedLC3B protein expression, indicating the promotion of the autophagy signaling pathway. Protein processingin the endoplasmic reticulum signaling pathway was downregulated in Gene Ontology (GO) andKyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, consistent with tealtimequantitative PCR and western blotting that showed YOD1, HSPA4L, DNAJC3, and HSP90AA1expression levels were dramatically decreased in HeLa cells treated with ginsenoside Rk1, with YOD1was the most significantly inhibited by ginsenoside Rk1 treatment. Conclusion: These findings indicate that the toxicity of ginsenoside Rk1 in HeLa cells can be explained bythe inhibition of protein synthesis in the endoplasmic reticulum and enhanced apoptosis, with YOD1acting as a potential target for cervical cancer treatment.
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