Sialoglycoprotein from Gadous morhua eggs improve high bone turnover activity via down-regulating BMP-2/Smads and Wnt/b-catenin signal pathways

Kai Wang,Lihua Han,Na Wang,Yiming Wang,Jingfeng Wang 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.5

The effect of sialoglycoprotein isolated from Gadous morhua eggs (Gm-SGP) on ovariectomized (OVX) induced osteoporosis, which is characterized by high bone turnover activity was investigated. Results revealed that Gm-SGP significantly increased bone mineral density, enhanced bone biomechanical properties and repaired the microstructure of the trabecular bone. Also, the treatment with Gm-SGP remarkably decreased biochemical marker contents or activities, such as serum BALP, PICP, BMP-2, TrACP, Cath-K, urine Ca and P, leading to the reduction in bone turnover. The elevation in the rate of bone formative process contributed in the increase of bone turnover. Both BMP-2/Smads and Wnt/b-catenin signaling pathways played an important role in osteogenesis. Gm-SGP suppressed the key factors expression in these two pathways such as BMP-2, Smad1, Smad4, Lrp-5b, Runx2, Osx, ALP, Col1, OCN and b-catenin. These findings might provide some theoretical basis for the application of Gm-SGP as a potential anti-osteoporotic drug or as functional food.

Intestinal Anti-Inflammatory Effects of Selenized Ulva pertusa Polysaccharides in a Dextran Sulfate Sodium-Induced Inflammatory Bowel Disease Model

Yifan Wang,Han Ye,Leke Qiao,Chunying Du,Zhengpeng Wei,Ting Wang,Jingfeng Wang,Ruizhi Liu,Peng Wang 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.3

The purpose of this study was to examine the alleviative effects of selenized polysaccharides from Ulva pertusa (ulvan-Se) on inflammatory bowel disease (IBD) in mice. The dextran sulfate sodium (DSS)-induced IBD mouse model was used to explore the protective effects of ulvan-Se on the intestinal mechanical and immune barrier. At doses less than 1208 mg/kg·bw ulvan-Se showed no significant damage to Institute of Cancer Research (ICR) mice in an acute toxicity test. The results showed that DSS destroyed the mechanical barrier, which includes epithelial cells, while ulvan-Se promoted mRNA expression of tight junction proteins (zonula occludens protein 1, occludin, and claudin-1) and inhibited the infiltration of white blood cells into the intestines. At 100 mg/kg·bw, ulvan-Se enhanced the antioxidant capacity of mice more effectively than the 50 mg/kg·bw ulvan-Se. Furthermore, ulvan-Se improved the intestinal immune barrier by increasing immunoglobulin A and immunoglobulin M, while regulating the levels of interleukin (IL)-1β, interferon-γ, and IL-4. Oral administration of ulvan-Se also suppressed tumor necrosis factor-α, IL-1β, IL-6, and cyclooxygenase-2 mRNA expression mediated by the nuclear factor kappa B pathway. Taken together, our findings reveal that ulvan-Se could be used as a potential alternative supplement for reducing intestinal inflammation in IBD.

Experimental and Analytical Behavior of Square CFDST Column Blind Bolted to Steel Beam Connections

Jingfeng Wang,Lei Guo 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.2

In recent years, steel or composite beam-column connections adopting blind fasteners have drawn increasing praise due to the rapid development of assembled steel structures. However, limited researches have paid attention to the experimental and analytical behavior of concrete filled double-skin steel tube (CFDST) column blind bolted joints. For investigating the structural performance and seismic behavior of this type of connection, cyclic tests on blind bolted joints to square CFDST columns were carried out to explore the effects of column hollow ratio and end plate type. Finite element analytical modelling of the semi-rigid joint was performed considering complex contact interactions and material models. Good agreement between the test and analytical results was observed in terms of the failure modes and the hysteretic behavior. Substantial parametric analyses were conducted on typical CFDST column connections to observe the influence of parameters including strength of steel tube, column hollow ratio and bolt pretension force. Furthermore, certain constructional measures commonly employed in engineering practice were also discussed. It was concluded that the CFDST column blind bolted to the steel beam joint has favorable seismic behavior and is feasible for application in high-intensity earthquake regions.

Seismic Response Investigation and Nonlinear Numerical Analysis of Cold-Formed Thin-Walled Steel Tube Truss Shear Walls

Jingfeng Wang,Wanqian Wang,Shuai Pang,Bo Yu 한국강구조학회 2019 International Journal of Steel Structures Vol.19 No.5

In order to satisfy the requirements of seismic capacity of cold-formed thin-walled steel structure in low- and multi-rise buildings, an innovative cold-formed thin-walled steel tube truss (CFSTT) shear wall sheathed with both-side oriented strand board (OSB) was proposed in this paper. Five full-scale specimens of CFSTT shear walls which composed of square cold-formed thin-walled steel tubes, tracks, galvanized V-shaped connectors and OSB panels were tested under low-cyclic loading. The seismic performance of CFSTT shear wall was evaluated in terms of hysteretic behavior, envelop curves, ductility and energy dissipation, etc. Then, a practical nonlinear simplifi ed analysis method of CFSTT shear walls under low-cyclic reversed lateral loading was proposed based on the principle of equivalent tie rod model. The double- and fourlimb lattice studs were also simplifi ed as single steel tubes. Besides, a pivot modeling approach was suggested to stimulate the sheathing-to-frame connection. Subsequently, the fi nite element models of CFSTT shear walls were established based on this simplifi ed method and verifi ed by the experimental data. Numerical and experimental results indicated that CFSTT shear walls exhibited excellent seismic performance, and the accuracy of the simplifi ed of lattice stud was verifi ed. Furthermore, the simplifi ed method proposed in this paper will highly facilitate conducting the nonlinear analysis for shear walls.

Cyclic Experimental Behavior of CFST Column to Steel Beam Frames with Blind Bolted Connections

Jingfeng Wang,Haitao Wang 한국강구조학회 2018 International Journal of Steel Structures Vol.18 No.3

This paper aims to investigate the seismic behavior of blind bolted end plate composite frames between square or circular concrete fi lled steel tubular (CFST) columns and steel beams. Two composite frames were tested under a constant axial load on the CFST columns and a lateral cyclic load on the frame. Each specimen composed of CFST columns and steel beams was selected to represent a plan frame in an assembly building. Failure pattern of the type of frames was analyzed to comprehend the structural response. The seismic resistance ability of the blind bolted CFST frames was also estimated in terms of hysteretic curves, ductility and energy dissipation etc. The eff ect of column section type and end plate type on the type of semi-rigid CFST frames was studied. The test results showed that at the same steel ratio of the column section, the bearing capacity and energy dissipation of square CFST frame were higher than those of circular CFST frame at ultimate state. Strain response of main members in each CFST frame was also estimated. The internal force analysis of the test CFST frames with semi-rigid connections was discussed and evaluated the eff ect of the bending moment distribution. It was concluded that the novel typed CFST frames exhibited excellent seismic performance and structural internal force redistribution. The experimental studies will be useful for design and application of the blind bolted CFST frames in fabricated steel structure building.

Global Lagrange Stability for Takagi-Sugeno Fuzzy Cohen-Grossberg BAM Neural Networks with Time-varying Delays

Jingfeng Wang,Lixin Tian,Zaili Zhen 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.4

This paper concerns the globally exponential stability in Lagrange sense for Takagi-Sugeno (T-S) fuzzy Cohen-Grossberg BAM neural networks with time-varying delays. Based on the Lyapunov functional method and inequality techniques, two different types of activation functions which include both Lipschitz function and general activation functions are analyzed. Several sufficient conditions in linear matrix inequality form are derived to guarantee the Lagrange exponential stability of Cohen-Grossberg BAM neural networks with time-varying delays which are represented by T-S fuzzy models. Finally, simulation results demonstrate the effectiveness of the theoretical results.

Seismic Response Investigation and Analyses of End Plate Moment-Resisting CFST Frames Under Pseudo-Dynamic Loads

Jingfeng Wang,Xuebei Pan,Xiao Peng,Jiaxin Wang 한국강구조학회 2019 International Journal of Steel Structures Vol.19 No.6

This paper reported an innovative semi-rigid composite frame comprised of concrete-fi lled steel tubular (CFST) columns and H-shaped steel beams assembled using fl ush or extended end plates with blind fasteners. Two specimens of two-story single-bay end plate moment-resisting square or circular CFST frames were investigated by a series of pseudo-dynamic tests. To analyze dynamic response of this type of structure under the earthquake action, time history response and story drift were investigated. The infl uence of the column section type and the end plate type on seismic performance of semirigid moment-resisting CFST frames under diff erent earthquake loading levels were also discussed according to hysteretic behavior, rigidity degradation and energy dissipation capacity. The experimental and analytical results indicated that at the same section width and steel ratio of the column, the rigidity of end plate moment-resisting square CFST frame was greater than that of circular CFST frame, while the story drift of square CFST frame was smaller than that of circular CFST frame. The type of end plate moment-resisting CFST frames with blind fasteners, which can provide good seismic behavior and energy dissipation ability, can be well used in a seismic region.

Analytical Performance and Design of CECFST Column Assembled to PEC Beam Joint

Jingfeng Wang,Zhihan Hu,Lei Guo,Bo Wang,Yonggan Yang 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.7

Concrete-encased concrete-filled steel tube (CECFST) columns and partially encased composite (PEC) beams are both steel-concrete composite members which have outstanding advantages and have been applicated in some engineering constructions. This paper makes an attempt to employ blind bolting technique to assemble CECFST columns to PEC beams. For the purpose of exploring the mechanical performance of this type of assembled joints, firstly, a nonlinear finite element (FE) model was established and validated, where complicated contact interaction was taken into consideration. Plenty of parametric analysis were adopted to find out the effect of ten parameters on strength and initial stiffness of the assembled joint. Numerical analysis showed that mechanic behavior is notably affected by axial load ratio, width and thickness of end-plate, bolt pretension force, bolt diameter, and steel ratio of CECFST column. Full-range analysis was then carried out to observe stress and strain developments and failure modes. Load transfer mechanism in CECFST column was also studied. Design methods to estimate the flexural capacity and initial stiffness of the connections were proposed in accordance with the component models. In comparison, it could be concluded that the calculation results fit well with the analytical results, which confirms that the design formulas could provide a favorable basis in practical design.

Experimental and Numerical Analyses of Blind Bolted Momentresisting Composite Joints to CFST Columns with RC Slab

Jingfeng Wang,Yue Pang 한국강구조학회 2017 International Journal of Steel Structures Vol.17 No.3

In this paper, experimental and numerical analyses were conducted to investigate the structural properties and failure modes of the blind bolted flush or extended end plate joints between concrete-filled steel tubular (CFST) columns and composite beams. The experimental program and the main results of the tests were elaborated. Four full-scale subassemblages beam-tocolumn exterior joints to hot-rolled and cold-formed CFST columns were tested. The failure modes and moment-rotation relation curves of the joints were evaluated. The test results showed that all specimens exhibited favorable strength and stiffness, and larger deformation capacities. Meanwhile, finite element analysis (FEA) modeling of the specimens was built and analyzed. The results obtained from the FEA modeling were satisfactory agreement with the experimental tests results. Then, an extensive parametric study was conducted to investigate the influence of various parameters on the moment capacity and rotation stiffness of composite joints under positive or negative moment. The experimental studies and numerical analyses can improve the design of the typed joints to be used in moment-resisting composite frames

Seismic Behavior Investigation on Blind Bolted CFST Frames with Precast SCWPs

Jingfeng Wang,Qihan Shen,Beibei Li 한국강구조학회 2018 International Journal of Steel Structures Vol.18 No.5

To explore seismic behavior of blind bolted concrete-fi lled steel tube (CFST) frames infi lled with precast sandwich composite wall panels (SCWPs), a series tests of blind bolted square CFST frames with precast SCWPs under lateral low-cyclic loading were conducted. The infl uence of the type of wall concrete, wall-to-frame connection and steel brace setting, etc. on the hysteretic curves and failure modes of the type of composite structure was investigated. The seismic behavior of the blind bolted CFST frames with precast SCWPs was evaluated in terms of lateral load–displacement relation curves, strength and stiff ness degradation, crack patterns of SCWPs, energy dissipation capacity and ductility. Then, a fi nite element (FE) analysis modeling using ABAQUS software was developed in considering the nonlinear material properties and complex components interaction. Comparison indicated that the FE analytical results coincided well with the test results. Both the experimental and numerical results indicated that setting the external precast SCWPs could heighten the load carrying capacities and rigidities of the blind bolted CFST frames by using reasonable connectors between frame and SCWPs. These experimental studies and FE analysis would enable improvement in the practical design of the SCWPs in fabricated CFST structure buildings.