Endothelial Progenitor Cells’ Classification and Application in Neurological Diseases

Jing-jing Yuan,Jing Yang,Shi-lei Sun,Rui Zhang,Yu-ming Xu 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.4

The therapeutic effects of endothelial progenitor cells (EPCs) on ischemic stroke have been extensively studied in recent years. However, the differences in early EPCs and endothelial outgrowth cells (EOCs) are still unclear. Clarifications of their respective properties and specific functioning characteristics contribute to better applications of EPCs in ischemic diseases. In this review, we discuss cellular origin, isolation, culture, surface markers of early EPCs and EOCs and relevant applications in neurological diseases. We conclude that EOCs possess all characteristics of true endothelial progenitors and have potent advantages in EPC-based therapies for ischemic diseases. A number of preclinical and clinical applications of EPCs in neurological diseases are under study. More studies are needed to determine the specific characteristics of EPCs and the relevant mechanisms of EPCs for neurological diseases.

MiR-886-5p Inhibition Inhibits Growth and Induces Apoptosis of MCF7 Cells

Zhang, Lei-Lei,Wu, Jiang,Liu, Qiang,Zhang, Yan,Sun, Zhu-Lei,Jing, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.

Bonding Behavior of TRC-Confined Concrete and Reinforcement under Chloride Erosion Environment

Lei Jing,Shiping Yin,Henglin Lv 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.3

The improved effect of the bonding behavior of concrete and reinforcement is a critical indicator for estimating the validity of strengthening measures. The bonding behavior of unconfined or textile reinforced concrete (TRC)-confined concrete and a plain or deformed reinforcement was comparatively analyzed via the pull-out tests, and the influence of chloride wet-dry cycles also was considered on the bonding behavior. Moreover, the bonding behavior was further revealed from the view of energy. The study results indicated that the bonding failure of specimens with a deformed reinforcement was changed after TRC confinement, showing a certain ductile failure characteristic, and the integrity of the confined specimens was ensured at failure. The impact of chloride attack on the bonding behavior of concrete and a plain or deformed reinforcement was different under the unconfined or TRC-confined condition. The deterioration mechanism of the bonding behavior needs to be further investigated when the reinforcement is corroded seriously. The results from the energy analysis are consistent with the pull-out test results.

Research on Large‑scale Photovoltaic Planning Based on Risk Assessment in Distribution Network

Lei Wang,Minyu Yuan,Fan Zhang,Xuli Wang,Jing Ma,Lei Dai,Xianjun Qi,Rui Bi 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.3

Risks caused by large amounts of distributed photovoltaic (PV) feeding into distribution networks, have an impact on PV planning, which has become a critical consideration for distribution networks’ operation. In this paper, a large-scale PV planning method is proposed which based on risk assessment in distribution networks. Cluster division is used to group large-scale distributed PVs. A risk assessment is performed on the distribution network, considering the correlation between PVs and loads. The efects of load fuctuations, PV quantity, capacity and location on the operational risk of the distribution network are analyzed and discussed. A large-scale PV planning model is established with the goal of maximizing the comprehensive benefts, considering the penalty cost of node voltage over-limit and branch power fow over-limit risk. Finally, the genetic algorithm is used to solve the planning model. The simulation results demonstrate the efectiveness of the proposed method.

Overexpression of the AtSTK Gene Increases Salt, PEG and ABA Tolerance in Arabidopsis

Lei Bing,Cui-Cui Feng,Jing-Lan Li,Xiao-Xu Li,Baocun Zhao,Yin-Zhu Shen,Zhan-Jing Huang,Rong-Chao Ge 한국식물학회 2013 Journal of Plant Biology Vol.56 No.6

AtSTK (At5g02800), which is a serine-threonineprotein kinase gene of Arabidopsis thaliana, was cloned, andits function was studied. The study found that the overexpressionof AtSTK could significantly improve the ability of A. thaliana to tolerate salt, PEG, and ABA stresses. RT-PCRanalysis revealed that the expression of the AtSTK genecould be obviously induced by salt, PEG, and ABA. Theexamination of the physiological characteristics showed thatthe overexpression of AtSTK in Arabidopsis significantlyreduced the plasma membrane permeability, significantlyincreased the proline content, and decreased the MDA content. These changes may reflect the physiological mechanismsthrough which AtSTK overexpression improves stress resistancein Arabidopsis. In addition, the overexpression of the AtSTKgene significantly antagonised the inhibitory effect of highconcentrations of exogenous ABA on Arabidopsis seedgermination. The subcellular localisation results showed thatAtSTK is located in both the cytosol and the nucleus. Theexamination of its tissue-specific expression showed thatAtSTK is expressed in various Arabidopsis tissues and isparticularly strongly expressed in the vessels. The signallingpathway analysis indicated that AtSTK might transfer thesalt stress signal in Arabidopsis through the MAPK pathway.

Preparation and Properties of Alginate/Polyaspartate Composite Hydrogels

Lei, Jing,Kim, Ji-Heung,Jeon, Young-Sil The Polymer Society of Korea 2008 Macromolecular Research Vol.16 No.1

This study examined the swelling behavior and in vitro release of a model drug, tetracycline-HCl, from alginate and alginate-polyaspartate (Alg-PASP) composite gel beads. The alginate and Alg-PASP composite beads were prepared using an ionic crosslinking method with aqueous $Ca^{2+}$. Their microporous morphology was observed by scanning electron microscopy. The swelling ratio of the beads in different media varied according to their composition, cross-linking density ($Ca^{2+}$ concentration), and pH of the aqueous medium. The in vitro release experiment of the tetracycline-HCl encapsulated beads in different media suggests that the release of the drug is governed mainly by the swelling properties of the polymer network. The presence of PASP was found to significantly influence the swelling properties and drug release profile.

Preparation and Properties of Alginate/Polyaspartate Composite Hydrogels

Jing Lei,Ji Heung Kim,Young Sil Jeon 한국고분자학회 2008 Macromolecular Research Vol.16 No.1

Vibration characteristics of functionally graded carbon nanotube-reinforced composite double-beams in thermal environments

Jing-Lei Zhao,Xu Chen,Gui-Lin She,Yan Jing,Ru-Qing Bai,Jin Yi,Hua-Yan Pu,Jun Luo 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.43 No.6

This paper presents an investigation on the free vibration characteristics of functionally graded nanocomposite double-beams reinforced by single-walled carbon nanotubes (SWCNTs). The double-beams coupled by an interlayer spring, resting on the elastic foundation with a linear layer and shear layer, and is simply supported in thermal environments. The SWCNTs gradient distributed in the thickness direction of the beam forms different reinforcement patterns. The materials properties of the functionally graded carbon nanotube-reinforced composites (FG-CNTRC) are estimated by rule of mixture. The first order shear deformation theory and Euler-Lagrange variational principle are employed to derive the motion equations incorporating the thermal effects. The vibration characteristics under several patterns of reinforcement are presented and discussed. We conducted a series of studies aimed at revealing the effects of the spring stiffness, environment temperature, thickness ratios and carbon nanotube volume fraction on the nature frequency.

전복내장추출물의 항산화 및 human dermal fibroblasts에 대한 항피부노화 효과

이정뢰 ( Jing Lei Li ),동도 ( Tao Tong ),고두옥 ( Du Ock Ko ),정동옥 ( Dong Ok Chung ),정원철 ( Won Chul Jeong ),김지은 ( Ji Eun Kim ),강성국 ( Seong Gook Kang ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2012 한국식품저장유통학회지 Vol.19 No.4

In this study, the anti-oxidant and anti-elastase activities of four abalone viscera extracts were investigated to screen the most promising extract. This extract was further studied in terms of its anti-skin-aging properties. In the DPPH-scavenging assay, the Tris-HCl extract showed a 58.60±0.88% radical-scavenging activity, which was followed closely by the ethanol extract that had a 55.40±0.62% scavenging activity. In the anti-elastase assay, however, the ethanol extract showed the significantly highest elastase inhibition activity. Furthermore, none of the extracts had a harmful effect on the human dermal fibroblast, as revealed in the MTT assay. In the cell study, the effect of the ethanol extract at various concentrations on the human dermal fibroblast was investigated. At the 10 μg/mL concentration, the ethanol extract boosted the pro-collagen type I synthesis to 705.30±3.06 ng/mL and reduced the MMP-1 to 54.30±0.80 ng/mL, which was considered the optimum concentration. This is the first study that focused on the anti-oxidant and anti-skin-aging effects of abalone viscera extract. Its results may provide fundamental data for further study.

Fotemustine, Teniposide and Dexamethasone in Treating Patients with CNS Lymphoma

Wu, Jing-Jing,Wang, Xin-Hua,Li, Ling,Li, Xin,Zhang, Lei,Sun, Zhen-Chang,Fu, Xiao-Rui,Ma, Wang,Chang, Yu,Zhang, Xu-Dong,Han, Li-Juan,Zhang, Ming-Zhi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Purpose: We developed and evaluated a regimen including fotemustine, teniposide and dexamethasone (FTD) for treating patients with central nervous system (CNS) lymphoma based on pharmacokinetic properties of individual agents and in combination. Patients and Methods: In a comparison study, 8 patients with primary CNS lymphoma (PCNSL) and 8 with secondary CNS lymphoma (SCNSL) were treated with FTD (comprising fotemustine 100 mg/m2, 1h infusion, day 1; teniposide 60 mg/m2, >0.5 h infusion, on day 2, 3, 4; dexamethasone 40 mg, 1h infusion, on day 1, 2, 3, 4 and 5; and methotrexate 12 mg, cytosine arabinoside 50 mg plus dexamethasone 5 mg intrathecally, on day 2 and 7). Cycles were repeated every 3 weeks. After response assessment, patients received whole brain radiotherapy. Results: Of the 8 PCNSL patients, 4 (50%) achieved CR and 3 (38%) PR, an overall response rate of 88%. Four patients (50%) were in continuing remission at the end of this study after a median follow-up of 30 months (range 10 to 56 months). Of the 8 SCNSL patients the overall response rate was 63% (CR+PR: 38%+25%). All responses were achievable with predictable toxicity mainly reflecting reversible myelosuppression. Conclusion: This study suggests that FTD could be an effective treatment for CNS lymphoma, and is worthy of further evaluation.