http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Zhu, Guan Qun,Jeon, Seung Hwan,Bae, Woong Jin,Choi, Sae Woong,Jeong, Hyun Cheol,Kim, Kang Sup,Kim, Su Jin,Cho, Hyuk Jin,Ha, U. Syn,Hong, Sung Hoo,Lee, Ji Youl,Kwon, Eun Bi,Kim, Sae Woong Hindawi 2018 Stem cells international Vol.2018 No.-
<P><B>Background</B></P><P> Mesenchymal stem cell therapy (MSCT) and defocused low-energy shock wave therapy (ESWT) has been shown to ameliorate erectile dysfunction (ED). However, the interactions and effects of action between MSCT and ESWT remain poorly understood. In this study, we investigated the mechanisms of combination therapy with MSCT and ESWT in a rat model of diabetic ED. </P><P><B>Materials and Methods</B></P><P> Eight-week-old male Sprague-Dawley rats were randomly divided into 2 parts. Diabetic rats induced by streptozotocin (65 mg/kg) were randomly divided into 4 groups: (1) DM control group, (2) DM + ESWT group, (3) DM + MSCT group, and (4) DM + ESWT + MSCT group. The sham group was a normal control group (without streptozotocin). MSCT and (or) ESWT were, respectively, administered to each group according to the proposal for 8 weeks. Immediately after recording of intracavernous pressure (ICP), the penis was then harvested for histologic analysis, ELISA, and Western blotting. </P><P><B>Results</B></P><P> The ratio of ICP/MAP was significantly higher in the DM + ESWT + MSCT group than in ESWT or MSCT treated group (<I>P</I> < 0.05). Also, the treatment stimulated angiogenesis and vasodilatation in the corpus cavernosum (<I>P</I> < 0.05). ESWT increased the quantity of MSCs in the corpus cavernosum and also induced MSCs to express more VEGF in vitro and vivo (<I>P</I> < 0.05) which activated the PI3K/AKT/mTOR and NO/cGMP signaling pathways in the corpus cavernosum. The combination approach stimulated autophagy and decreased apoptosis in the corpus cavernosum. NGF and BDNF expressions were higher in the DM + ESWT + MSCT group than in the DM control group (<I>P</I> < 0.01). Furthermore, the treatment promoted the MSC recruitment by inducing penile tissues to express more PECAM and SDF-1. </P><P><B>Conclusions</B></P><P> Combination of LI-ESWT and MSCT can get a better result than a single treatment by expressing more VEGF which can take part in autophagy by triggering the PI3K/AKT/mTOR signaling pathway. This cooperative therapy would provide a new research direction in ED treatment for the future.</P>
Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance.
Zhu, Jianhua,Jeong, Jae Cheol,Zhu, Yanmei,Sokolchik, Irina,Miyazaki, Saori,Zhu, Jian-Kang,Hasegawa, Paul M,Bohnert, Hans J,Shi, Huazhong,Yun, Dae-Jin,Bressan, Ray A National Academy of Sciences 2008 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.105 No.12
<P>Histone modification in chromatin is one of the key control points in gene regulation in eukaryotic cells. Protein complexes composed of histone acetyltransferase or deacetylase, WD40 repeat protein, and many other components have been implicated in this process. Here, we report the identification and functional characterization of HOS15, a WD40-repeat protein crucial for repression of genes associated with abiotic stress tolerance through histone deacetylation in Arabidopsis. HOS15 shares high sequence similarity with human transducin-beta like protein (TBL), a component of a repressor protein complex involved in histone deacetylation. Mutation of the HOS15 gene renders mutant plants hypersensitive to freezing temperatures. HOS15 is localized in the nucleus and specifically interacts with histone H4. The level of acetylated histone H4 is higher in the hos15 mutant than in WT plants. Moreover, the stress inducible RD29A promoter is hyperinduced and associated with a substantially higher level of acetylated histone H4 in the hos15 mutant under cold stress conditions. Our results suggest a critical role for gene activation/repression by histone acetylation/deacetylation in plant acclimation and tolerance to cold stress.</P>
Zhu, Jianhua,Fu, Xinmiao,Koo, Yoon Duck,Zhu, Jian-Kang,Jenney Jr., Francis E.,Adams, Michael W. W.,Zhu, Yanmei,Shi, Huazhong,Yun, Dae-Jin,Hasegawa, Paul M.,Bressan, Ray A. American Society for Microbiology 2007 Molecular and cellular biology Vol.27 No.14
<B>ABSTRACT</B><P>The myristoylated calcium sensor SOS3 and its interacting protein kinase, SOS2, play critical regulatory roles in salt tolerance. Mutations in either of these proteins render <I>Arabidopsis thaliana</I> plants hypersensitive to salt stress. We report here the isolation and characterization of a mutant called <I>enh1-1</I> that enhances the salt sensitivity of <I>sos3-1</I> and also causes increased salt sensitivity by itself. <I>ENH1</I> encodes a chloroplast-localized protein with a PDZ domain at the N-terminal region and a rubredoxin domain in the C-terminal part. Rubredoxins are known to be involved in the reduction of superoxide in some anaerobic bacteria. The <I>enh1-1</I> mutation causes enhanced accumulation of reactive oxygen species (ROS), particularly under salt stress. ROS also accumulate to higher levels in <I>sos2-1</I> but not in <I>sos3-1</I> mutants. The <I>enh1-1</I> mutation does not enhance <I>sos2-1</I> phenotypes. Also, <I>enh1-1</I> and <I>sos2-1</I> mutants, but not <I>sos3-1</I> mutants, show increased sensitivity to oxidative stress. These results indicate that ENH1 functions in the detoxification of reactive oxygen species resulting from salt stress by participating in a new salt tolerance pathway that may involve SOS2 but not SOS3.</P>
Bae, Woong Jin,Zhu, Guan Qun,Choi, Sae Woong,Jeong, Hyun Cheol,Bashraheel, Fahad,Kim, Kang Sup,Kim, Su Jin,Cho, Hyuk Jin,Ha, U Syn,Hong, Sung Hoo,Lee, Ji Youl,Oh, Hyun-A,Koo, Hye Cheong,Kim, Do Ram,Hw Hindawi 2017 Oxidative medicine and cellular longevity Vol.2017 No.-
<P>The Korean herbal formulation Ojayeonjonghwan is used for improving late-onset hypogonadism (LOH) symptoms such as erectile dysfunction (ED). A previous research suggested that a modified Ojayeonjonghwan (KH-204) could be used as an alternative to the treatment for ED. The pharmacological effects were examined in different conditions, including in vitro and in vivo. We measured the survival rate of TM3 Leydig cells under the oxidative stress condition. The s.c. injection of leuprorelin was used to induce androgen deprivation. We measured serum testosterone levels, oxidative stress, and apoptosis. The results of the treatment by KH-204 (1) preserved TM3 cells from oxidative stress by improving the expression of nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1); (2) lowered the expression of transforming growth factor-beta (TGF-<I>β</I>) 1/SMAD; (3) increased the average of serum testosterone in androgen-deprived male rats; (4) kept the activation of spermatogenesis; (5) upgraded the contents of 8-hydroxy-20-deoxyguanosine (8-OHdG) and degraded the contents of superoxide dismutase (SOD); and (6) reduced apoptosis. We studied that KH-204 improved testicular dysfunction in LOH. It is likely, at least in part, to degrade oxidative stress through the Nrf2/HO-1 pathway. These findings may offer credible evidences for the use of new alternative therapies to treat LOH.</P>
Sae Woong Choi,Seung Hwan Jeon,Eun Bi Kwon,Guan Qun Zhu,Kyu Won Lee,Jin Bong Choi,Hyun Cheol Jeong,Kang Sup Kim,Sang Rak Bae,Woong Jin Bae,Su Jin Kim,Hyuk Jin Cho,U-Syn Ha,Sung Hoo Hong,Sung Yeoun Hwa 대한남성과학회 2019 The World Journal of Men's Health Vol.37 No.1
Purpose: Testosterone replacement therapy is an effective treatment for late-onset hypogonadism (LOH) despite a few con-traindications and side-effects. The aim of this study was to determine whether modified Ojayeonjonghwan (KH-204, Korean herbal formula) improved LOH. KH-204 is a strong antioxidant herbal formula. We evaluated the effect of Korean herbal pre-scription on androgen receptor (AR) expression in an aged rat model of LOH. Materials and Methods: Eighteen-month-old rats were used as aged LOH rat models. Eighteen Sprague-Dawley rats were ran-domly divided into three equal groups of six animals each and treated with one of the following: 1) normal control group (oral administration with distilled water, n=6), 2) KH-204 200 group (oral administration with 200 mg/kg of KH-204, n=6), and 3) KH-204 400 group (oral administration with 400 mg/kg of KH-204, n=6). After four weeks of treatment (once daily, distilled water or KH-204), serum testosterone levels, changes in testicular and epididymal weight, Western blotting analysis of AR ex-pression and measurement of oxidative stress were examined. Results: Treatment with the herbal formulation KH-204 200 mg/kg and 400 mg/kg (1) increased the weights of testis and epi-didymis; (2) increased the level of serum testosterone; (3) increased the level of superoxide dismutase and reduced the level of 8-hydroxy-20-deoxyguanosine; and (4) upregulated AR expression in testicular tissue. Conclusions: KH-204 might be an effective alternative for LOH. It improves antioxidant mechanisms and increases testicular AR expression without side-effects.
Zhu, Yingfang,Schluttenhoffer, Craig M.,Wang, Pengcheng,Fu, Fuyou,Thimmapuram, Jyothi,Zhu, Jian-Kang,Lee, Sang Yeol,Yun, Dae-Jin,Mengiste, Tesfaye American Society of Plant Biologists 2014 The Plant cell Vol.26 No.10
<P>This work explores the intriguing roles of Mediator subunit CYCLIN-DEPENDENT KINASE8 (CDK8) in plant immune responses to fungal infection. CDK8 regulates jasmonate-responsive gene expression and cuticle development via interactions with MEDIATOR COMPLEX SUBUNIT25 and transcription factor WIN1, respectively, while other interactions suggest evolutionary conservation of the Mediator kinase module.</P><P>CYCLIN-DEPENDENT KINASE8 (CDK8) is a widely studied component of eukaryotic Mediator complexes. However, the biological and molecular functions of plant CDK8 are not well understood. Here, we provide evidence for regulatory functions of <I>Arabidopsis thaliana</I> CDK8 in defense and demonstrate its functional and molecular interactions with other Mediator and non-Mediator subunits. The <I>cdk8</I> mutant exhibits enhanced resistance to <I>Botrytis cinerea</I> but susceptibility to <I>Alternaria brassicicola</I>. The contributions of CDK8 to the transcriptional activation of defensin gene <I>PDF1.2</I> and its interaction with MEDIATOR COMPLEX SUBUNIT25 (MED25) implicate CDK8 in jasmonate-mediated defense. Moreover, CDK8 associates with the promoter of <I>AGMATINE COUMAROYLTRANSFERASE</I> to promote its transcription and regulate the biosynthesis of the defense-active secondary metabolites hydroxycinnamic acid amides. CDK8 also interacts with the transcription factor WAX INDUCER1, implying its additional role in cuticle development. In addition, overlapping functions of CDK8 with MED12 and MED13 and interactions between CDK8 and C-type cyclins suggest the conserved configuration of the plant Mediator kinase module. In summary, while CDK8’s positive transcriptional regulation of target genes and its phosphorylation activities underpin its defense functions, the impaired defense responses in the mutant are masked by its altered cuticle, resulting in specific resistance to <I>B. cinerea</I>.</P>
Performance of a Large Volume Liquid Scintillation Detector for the Measurement of Fast Neutrons
Jing-Jun Zhu,J.I. Lee,곽정원,Jin Li,Ke-Jun Kang,M.J. Hwang,M.J. Lee,S.C. Kim,S.S. Myung,S.Y. Kim,Y.D. Kim,Yuan-Jing Li,J. Lee,김선기,방형찬,H.J. Kim,이현수,한인식,권영준 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.47 No.1
The KIMS collaboration is an experimental group searching for the Weakly Interacting Massive Particle (WIMP) which is one of the strongest candidates of dark matter. In a WIMP search experiment, the neutron is an important background because the nuclear recoil signal of a neutron is indistinguishable from that of a WIMP. We have constructed a 20-liter liquid scintillation detector to measure the neutron background. We present the performance of the neutron detector, as well as its structure and electronics system. We have achieved a good neutron/gamma separation anda good position measurement. A position dependent energy calibration has also been performed to correct the nonlinear response of the detector for different particle incident positions.
Zheng, Xiang-Guo,Kang, Jong-Seong,Kim, Yong,You, Young-Jae,Jin, Guang-Zhu,Ahn, Byung-Zun The Pharmaceutical Society of Korea 1999 Archives of Pharmacal Research Vol.22 No.4
Thirty-four glutathione conjugates of 5,8-dimethoxy-1,4-naphthoquinones (DMNQ) were synthesized and their structure was determined. The yield of GSH conjugate was dependent on size of alkyl group; the longer the size of alkyl group was, the lower was the yield. It was also found that the length of alkyl side chain influenced the chemical shift of quinonoid protons; the quinonoid protons of 2-glutathionyl DMNQ derivatives with R=H to propyl, 6.51-6.59 ppm vs. other ones with R=butyl to heptyl, 6.64-6.68 ppm. this was explained to be due to a folding effect of longer alkyl group. Glutathione (GSH) reacted with DMNQ derivative first to form a 1,4-adduct (2- or 3-glutathionyl-1,4-dihydroxy-5,8-dimethoxynaphthalenes) and then the adduct was autooxidized to 2- or 3-glutathionyl-DMNQ derivatives. Moreover, GSH reduced DMNQ derivatives to their hydrogenated products. It was suggested that such an organic reaction might play an important role for a study of metabolism or toxicity of DMNQ derivative sin the living cells.