Relationships of uPA and VEGF Expression in Esophageal Cancer and Microvascular Density with Tumorous Invasion and Metastasis

Jiang, Jian-Tao,Zhang, Lan-Fang,Zhou, Bin,Zhang, Shun-Qun,Li, Shao-Min,Zhang, Wei,Zhang, Jin,Qiao, Zhe,Kong, Ran-Ran,Ma, Yue-Feng,Chen, Sheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Objective: To investigate uPA and VEGF expression in esophageal cancer and relations with tumorous invasion and metastasis. Methods: Immunohistochemistry was used to detect uPA and VEGF expression in the normal epithelial tissue of esophageal mucosa and cancer tissue and detect CD34 labeled micrangium and analyze the relationships with clinical pathological features and tumor angiogenesis. Results: Positive rates for uPA and VEGF protein expression were significantly greater in esophageal cancer than normal epithelial tissue (P < 0.05), the two being linked (P <0.05). In addition, uPA and VEGF protein expression of the high microvessel density (MVD) group was significantly lower than in the low MVD group (P < 0.05), with relation to clinical pathological staging, differentiation and lymph node metastasis (P < 0.05). Conclusion: In esophageal cancer tissue, uPA and VEGF proteins are overexpressed and promote tumor angiogenesis, indicative of a poor prognosis.

Transcriptome analysis in different chieh‑qua cultivars provides new insights into drought‑stress response

Min Wang,Xiaoming He,Biao Jiang,Wenrui Liu,Yu’e Lin,Dasen Xie,Zhaojun Liang,Lihui Chen,Qingwu Peng 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.6

Drought, one of the crucial environmental constraints, seriously threats the quality and yield in chieh-qua. Therefore, cultivat-ing drought-tolerant variety is greatly necessary for its normal growth under water deficiency. However, at present, molecular knowledge on drought resistance is mostly unclear in chieh-qua. In the study, characteristics of two diverse genetic chieh-qua variety, A39 (drought-resistance) and H5 (drought-sensitivity), were analyzed. Under drought stress, H5 exerted high water loss rate, increased malonaldehyde (MDA) content, and decreased enzyme activity of glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) compared with A39. In addition, based on the transcriptome results, we obtained a total of 1821 (511 up-regulated and 1310 down-regulated) and 2114 (1282 up-regulated and 832 down- regulated) differentially expressed genes (DEGs) in the A39 versus H5 under normal and water-deficiency stress, respectively. Several DEGs involved in the cuticle synthesis (cytochrome P450 genes: CYP94A2, CYP86B1, CYP86A7), carbohydrate metabolism, and plant hormone signal transduction (small auxin-up RNA genes: SAUR32, SAUR72; JA-induced genes: TIFY 10A, TIFY 10C; ABA related genes: PYL2, PYL4) were explored and related to drought resistance. These expression patterns observed in the RNA-seq data were further confirmed with quantitative real-time PCR (qRT-PCR). In all, these results not only provided a new insight into analyzing genes of drought response, but also laid a foundation for isolating crucial genes involved in drought stress in chieh-qua.

Quantitative Assessment of the Association between ABC Polymorphisms and Osteosarcoma Response: a Meta-analysis

Chen, Xu,Jiang, Min,Zhao, Rui-Ke,Gu, Guo-Hao Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

Background: ABC proteins are one key type of transport superfamilies which undertake majority of drug transport, which affect the osteosarcoma response to chemotherapeutics. Previous studies have suggested the association between ABC polymorphisms and osteosarcoma response. However, the results of previous studies remain controversial. Therefore, we perform a meta-analysis to get a more precise estimation of this association. The association between ABC polymorphisms and osteosarcoma response was assessed by odds ratios (ORs) together with their 95% confidence intervals (CIs). Three polymorphisms of ABC including ABCB1 rs1128503, ABCC3 rs4148416 and ABCC2 rs717620 polymorphism were investigated. Overall, significant association was observed between ABCC3 rs4148416 polymorphism and osteosarcoma response under allele contrast (T vs. C: OR=1.73, 95%CI=1.09-2.74, P=0.019), homozygote comparison (TT vs. CC: OR=2.00, 95%CI=1.25-3.23, P=0.004), recessive genetic model (TT vs. TC/CC: OR=1.80, 95%CI=1.14-2.84, P=0.011) and dominant genetic model (TT/TC vs. CC: OR=1.70, 95%CI=1.20-2.42, P=0.003). Moreover, significant association was also observed in Caucasian population rather than Asian population for ABCB1 rs1128503 polymorphism. We conclude that ABCC3 rs4148416 polymorphism was significantly associated with poor osteosarcoma response and ABCB1 rs1128503 polymorphism was significantly associated with good osteosarcoma response in Caucasian population rather than Asian population.

Kocuria subflava sp. nov., isolated from marine sediment from the Indian Ocean

Jiang, Zhao,Zhang, Wei-Hua,Yuan, Chang-Guo,Chen, Jia-Yang,Cao, Li-Xiang,Park, Dong-Jin,Xiao, Min,Kim, Chang-Jin,Li, Wen-Jun Springer-Verlag 2015 Antonie van Leeuwenhoek Vol.108 No.6

Temporary formation of highly conducting domain walls for non-destructive read-out of ferroelectric domain-wall resistance switching memories

Jiang, Jun,Bai, Zi Long,Chen, Zhi Hui,He, Long,Zhang, David Wei,Zhang, Qing Hua,Shi, Jin An,Park, Min Hyuk,Scott, James F.,Hwang, Cheol Seong,Jiang, An Quan Nature Publishing Group, a division of Macmillan P 2018 NATURE MATERIALS Vol.17 No.1

Erasable conductive domain walls in insulating ferroelectric thin films can be used for non-destructive electrical read-out of the polarization states in ferroelectric memories. Still, the domain-wall currents extracted by these devices have not yet reached the intensity and stability required to drive read-out circuits operating at high speeds. This study demonstrated non-destructive read-out of digital data stored using specific domain-wall configurations in epitaxial BiFeO<SUB>3</SUB> thin films formed in mesa-geometry structures. Partially switched domains, which enable the formation of conductive walls during the read operation, spontaneously retract when the read voltage is removed, reducing the accumulation of mobile defects at the domain walls and potentially improving the device stability. Three-terminal memory devices produced 14 nA read currents at an operating voltage of 5 V, and operated up to T = 85 °C. The gap length can also be smaller than the film thickness, allowing the realization of ferroelectric memories with device dimensions far below 100 nm.

Applications of Hilbert-Huang transform to structural damage detection

Dung-Jiang Chiou,Wen-Ko Hsu,Cheng-Wu Chen,Chih-Min Hsieh,Jhy-Pyng Tang,Wei-Ling Chiang 국제구조공학회 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.39 No.1

This study investigates the feasibility of detecting structural damage using the HHT method. A damage detection index, the ratio of bandwidth (RB) is proposed. This index is highly correlated or approximately equal to the change of equivalent damping ratio for an intact structure incurring damage from strong ground motions. Based on an analysis of shaking table test data from benchmark models subjected to adjusted Kobe and El Centro earthquakes, the damage detection index is evaluated using the Hilbert-Huang Transform (HHT) and the Fast Fourier Transform (FFT) methods, respectively. Results indicate that, when the response of the structure is in the elastic region, the RB value only slightly changes in both the HHT and the FFT spectra. Additionally, RB values estimated from the HHT spectra vs. the PGA values change incrementally when the structure response is nonlinear i.e., member yielding occurs, but not in the RB curve from the FFT spectra. Moreover, the RB value of the top floor changes more than those from the other floors. Furthermore, structural damage is detected only when using the acceleration response data from the top floor. Therefore, the ratio of bandwidth RB estimated from the smoothed HHT spectra is an effective and sensitive damage index for detecting structural damage. Results of this study also demonstrate that the HHT is a powerful method in analyzing the nonlinear responses of steel structures to strong ground motions.

Adsorptive Granulocyte and Monocyte Apheresis in the Treatment of Ulcerative Colitis: The First Multicenter Study in China

( Ya-min Lai ),( Wei-yan Yao ),( Yao He ),( Xuan Jiang ),( Yu-bei Gu ),( Min-hu Chen ),( Yu-lan Liu ),( Yao-zong Yuan ),( Jia-ming Qian ) 대한소화기학회 2017 Gut and Liver Vol.11 No.2

Background/Aims: Patients with active ulcerative colitis (UC) have elevated levels of activated myeloid-derived leukocytes as a source of inflammatory cytokines. The selective deple-tion of these leukocytes by adsorptive granulocyte/monocyte apheresis (GMA) with an Adacolumn should alleviate inflammation, promote remission and enhance drug efficacy. However, studies have reported contrasting efficacy out-comes based on patients` baseline demographic variables. This study was undertaken to understand the demographic features of GMA responders and nonresponders. Methods: This was a multicenter study in China involving four institutions and 34 patients with active UC. Baseline conventional medications were continued without changing the dosage. The treatment efficacy was evaluated based on the endo-scopic activity index and the Mayo score. Results: Thirty of the 34 patients completed all 10 GMA treatment sessions. The overall efficacy rate was 70.59%. The receiver operating characteristic analysis showed that the area under the curve was approximately 0.766 for a Mayo score of ≤5.5 with 0.273 specificity and 0.857 sensitivity (Youden index, 0.584) for GMA responders. No GMA-related serious adverse events were observed. Conclusions: The overall efficacy of GMA in patients with active UC who were taking first-line medications or were corticosteroid refractory was encouraging. Addition-ally, GMA was well tolerated and had a good safety profile. (Gut Liver 2017;11:216-225)

Isoperistaltic side-to-side anastomosis for the surgical treatment of Crohn disease

Wenhao Chen,Junjie Zhou,Min Chen,Congqing Jiang,Qun Qian,Zhao Ding 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.103 No.1

Purpose: Increasing evidence has shown an association of surgical technique, particularly anastomotic configuration, with postoperative recurrence of CD. This pilot study aimed to evaluate short-term outcomes of isoperistaltic side-to-side anastomosis (ISSA) employed on Crohn disease (CD) patients. Methods: Data were retrieved from a prospectively maintained database. Postoperatively, all patients were followed up with close endoscopic (ileocolonoscopy) surveillance. Results: From January 2017 to May 2021, 30 patients diagnosed with CD who underwent ISSA were compared with 45 CD patients who underwent antiperistaltic side-to-side anastomosis (ASSA). The 2 groups were comparable in baseline demographics and clinical characteristics. No significant differences were observed between groups regarding postoperative safety issues, including anastomotic leak, abdominal/pelvic abscess, length of hospital stay, readmission rate within 30 days, etc. At postoperative 24th month, reduced endoscopic recurrence was observed in the ISSA group compared with that in the ASSA group (18 of 24, 75.0%, . 36 of 38, 94.7%; P = 0.024). Regarding surgical recurrence, there was 0% in the ISSA group . 4.4% (2 of 45) in the ASSA group (P = 0.510). Conclusion: In this study, we aimed to explore the influence of ISSA on postoperative recurrence in CD patients, and the preliminary results show that ISSA was technically safe and feasible, and appears to be effective in reducing postoperative recurrence in CD patients. However, our conclusion was underpowered due to small sample size and inadequate follow- up. We proposed ISSA be considered as another alternative option in the toolbox of inflammatory bowel disease surgeons when performing anastomosis on CD patients.

Effect of Heat Treatment on the Properties of a High Alloying Maraging Steel Fabricated by Laser Metal Deposition

Linjun Jiang,Changjun Chen,Min Zhang 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.1

Traditional maraging steels are not able to satisfy the development of modern industry. Optimizing the properties of maragingsteels is now a fascinating field. In this paper, a novel high alloying element maraging steel (HA-MS1) is successfullyprepared by laser metal deposition (LMD). Then differential scanning calorimetry (DSC) phase transformation analysis onthe as-deposited (AF) specimens was conducted and the reasonable aging heat treatment (AT) and solution + aging heattreatment (ST + AT) were subsequently proposed. These specimens were characterized by scanning electron microscopy(SEM), electron back-scattered diffraction (EBSD), X-ray diffraction (XRD), hardness test, wear test and potentiodynamicpolarization test for microstructure, phase, hardness, tribological and corrosive properties. The grain refinement and a largenumber of Ni3(Ti, Mo) intermetallic compounds in the matrix after AT resulted in superior hardness and wear resistance,while leading to poor corrosion resistance. Thereinto, specimens subjected to 460 °C AT possessed the ideal hardness andwear resistance of 638 HV and 1.75 × 10−6 mm3/Nm, respectively, with an increase of 68% and 118% compared to theas-deposited specimens. The austenite ( ) content of the ST + AT specimens increased drastically, reaching 23.1% for the820 °C ST + 460 °C AT specimens. This phenomenon resulted in a decreasing compatibility of mechanical properties thanthe AT specimens, but the specimens aged at 490 °C after solution treatment at 820 °C had the best corrosion resistance,mainly due to their good surface and more densification.

Secondary Metabolites with Anti-complementary Activity from the Stem Barks of Juglans mandshurica Maxim

( Zi-jiang Li ),( Shilin Chen ),( Xiang-hao Yang ),( Rui Wang ),( Hee-jeong Min ),( Lei Wu ),( Chuan-ling Si ),( Young-soo Bae ) 한국목재공학회 2018 목재공학 Vol.46 No.2

Juglans mandshurica is a fast growing hard species, which is a tree in family of Juglandaceae and has a wide distribution in China, Korea and eastern Russia. Plant materials from J. mandshurica have extensively been used in folk medicines to prevent or cure gastric, esophageal, lung and cardiac cancer. As one chain of our searching for anticomplementary agents from natural sources, two epimeric ellagitannins, [2,3-O-4,4′,5,5′,6,6′,-hexahydroxydiphenoyl (HHDP))-(α,β)-D-glucose] (I) and pedunculagin (II) were purified from 70% acetone extracts of the stem barks of J. mandshurica by Thin Layer Chromatography and Sephadex LH-20 column chromatography approaches. The chemical structures of the isolated compounds were characterized by MS, NMR, and a careful comparation with published literatures. The epimeric ellagitannins I and II exhibited inhibitory properties against a classical pathway of complementary system with 50 % inhibitory concentrations (IC₅₀) values of 65.3 and 47.7 μM, respectively, comparing with riliroside (IC₅₀=104 μM) and rosmarinic acid (IC₅₀=182 μM), which were used as positive controls. Thus, the work indicated both the two secondary metabolites possess excellent inhibitory activity and might be developed as potential anticomplementary chemicals.