http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
겨우살이 첨가 김치의 발효양상과 항돌연변이 및 암세포성장저해효과
최선미,길정하,전영수,박건영 부산대학교 김치연구소 2003 김치의 과학과 기술 Vol.9 No.-
Fermentation characteristics, and antimutagenic and anticancer effects of mistletoe added kimchi were studied. The changes in pH, acidity and the value of prevalence test of the mistletoe added kimchi during the fermentation were determined. Ames antimutagenicity test against aflatoxin B_(1) (AFB_(1)) and N-methyl-N'nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA100, and MTT assay using HT-29 human colon carcinoma cells and AGS human gastric adenocarcinoma cells were employed to test the chemopreventive effect of the kimchis. Mistletoe added kimchis were fermented at 15℃ for 1 days and then ripened at 5℃ to reach optimum pH of 4.3. Mistletoe added kimchis fermented slowly compared to the control kimchi when the changes of pH and acidity of the kimchis were compared. Sour flavor of mistletoe in the kimchis was decreased during the fermentation. Mistletoe added kimchis exhibited higher inhibitory effect against the mutagenicities induced by AFB_(1) and MNNG in the Ames test than the control kimchi. Especially, 2% mistletoe added kimchi showed the highest inhibitory effect (75%, 68%) in the Ames test (p<0.05), Two % mistletoe added kimchi showed the highest anticancer effect against HT-29 human colon cells (p<0.05). Mistletoe added kimchis exhibited significantly higher anticancer effect than the control kimchi in AGS cells, too, however, there was no significant different effect among the different levels of mistletoe (0.5∼2.0%) addition (p<0.05). It can be concluded that addition of mistletoe in kimchi increased preservation period of the kimchi and enhanced antimutagenic and anticancer effects of the kimchi.
2-Bromoacetylnaphthalene을 螢光誘導體化劑로 利用한 Alclofenac의 HPLC 分析에 관한 硏究
李允中,曺正吉,河仁植,金容熙 成均館大學校 科學技術硏究所 1990 論文集 Vol.41 No.1
A simple, rapid and high sensitive method for determination of alclofenac is described. 2-Bromoacetylnaphthalene was used as the pre-column fluorescent derivatizing reagent for high performance liquid chromatography. Alclofenac was derivatized quantitatively into fluorescent compound by reacting with 2-bromoacetylnaphthalene in the presence of 18-crown-6 ether in acetonitrile. The optimum conditions for the derivatization such as concentration of KOH, 18-crown-6 and 2-bromoacetylnaphthalene, reaction temperature and reaction time were investigated. The structure of alcolofenac derivative was confirmed from IR, NMR and mass spectra. The fluorescence properties of alclofenac derivative were examined. The derivative was separated on a reverse phase column (Lichrosorb RP-8) in isocratid elution mode using the secondary mixture of acetonitrile and water as the mobile phase. The effluent was monitored by fluorometer.(Ex. 303, Em. 418 nm). The calibration plots for the peak area versus concentration of alclofenac observed to be linear(r =0.999).
Effects of Kimchi on Stomach and Colon Health of Helicobacter pylori-Infected Volunteers
Kil, Jeung-Ha,Jung, Keun-Ok,Lee, Hyo-Sun,Hwang, In-Kyung,Kim, Yun-Jin,Park, Kun-Young The Korean Society of Food Science and Nutrition 2004 Preventive Nutrition and Food Science Vol.9 No.2
The effects of kimchis intake on Helicobacter pylori infection in the stomach, the counts of lactic acid bacteria in the large intestine, and bacterial enzymes ($\beta$-glucosidase, $\beta$-glucuronidase) and pH in feces were examined. A total of 20 participants (age range 34 ∼ 57) were assessed for H. pylori infection status by Be urea breath test. Fourteen participants were eliminated because they were H. pylori-negative. This study consisted of 4 consecutive phase, each of which lasted 4 weeks. Three hundred grams of kimchi were administered to H. pylori-infected subjects during the kimchi phase, followed by 4 weeks of control phase. During the control phase, subjects consumed 60 g of kimchi, the minimum amount in their customary diets. All participants were found to be H. pylori-positive during all experimental periods. During the kimchi phase, delta over baseline (DOB) level was lower than during the control phase, although significant difference between the kimchi and control phases were not found (p=0.9439). However, the counts of Lactobacillus sp. and Leuconostoc sp. significantly (p < 0.0005) increased during the kimchi phase. $\beta$-Glucosidase and $\beta$-glucuronidase activities and pH were significantly decreased by kimchi intake compared to control (p=0.000l). These results suggested that kimchi consumption did not show any therapeutic effect on H. pylori in the stomach. However, kimchi seemed to be a good food for colon health, since it increased the beneficial bacteria such as lactobacillus and decreased toxic enzyme ($\beta$-glucosidase and $\beta$-glucuronidase) activity and pH.
In Vivo Antitumor Effects of Ready-to-Eat Raw Powdered Diets
Kil,Jeung Ha,Park,Kun Young 한국생명과학회 2001 한국생명과학회 학술발표회 Vol.33 No.-
Antitumor effects of ready-to -eat raw powdered diets were tested using sarcoma-180 cells transplanted Balb/c mouse. The raw diets used in this study were cancer preventive diets which were developed in our laboratory (Diet I, II) and commercial ready-to-eat raw powdered diets(Diet A, B) in Korea. The cancer preventive raw diet recipes were prepared using the food exchange system, and were mixed with various ingredients such as Brown rice, Job's tears, sorghum(cereals group), black soybean(legumes group), perilla seeds(oil seeds group), pine needle, Angelica utilis, purslane, kale, sea tangle and small water dropwort(vegetables group). The tumor formation, hepatic glutathione S-transferase(GST) activity and glutathione(GSH) levels were determined from the sarcoma-180 cell transplanted mice that were treated with methanol extracts from various raw diets. The tumor growth induced by the sarcoma-180 cells were significantly suppressed in the mice that fed the cancer preventive diets. The hepatic GST activity and GSH level were shown to be increased in these groups(p<0.05). The cancer preventive raw diets(Diet I and II) exhibited higher antitumor effect than the commercial raw diets(Diet A and B). Among the diet groups, Diet II was the most effective. The increased antitumor effect of the Diet II is probably due to the higher contents of cereal group and the pine needle.
Yong Ha Chu(朱勇河),Jeung Haing Oh(吳正行),Je Cheon Chae(蔡濟天),Yeaul Kyu Seung(成烈圭),Beom Young Son(孫範永),Kil Woong Chung(鄭吉雄) 한국육종학회 1996 한국육종학회지 Vol.28 No.3
This experiment was conducted to select useful breeding materials among eighty lines or varieties of summer type soybeans tested in the experiment field, DanKook University, Cheonan in 1993. Fourty-four to sixty-two days were observed as the range of days from planting to flowering and the average was 53 days. The range of days from planting to maturity was 112 to 127 days and the average was 117 days. Eighty-six percentage of tested soybean materials was white color of flower and green color of hypocotyl, while the remains were purple in both. Soybean entries with ovate, long circular and circular type of leaf were 6%, 73% and 21%, respectively. Large, medium and small leaf in the leaf area were 34%, 32% and 34%, respectively. Light green leaf color was observed as 15%, and green and dark green color were 70% and 15% among tested soybeans, respectively. Soybeans with tan pods were observed as 49%, brown and dark brown color were 14% and 37%. Erect type of pubescence was 36%, whereas curly and appressed type were 54% and 10%, respectively. Sparse, normal and dense pubescence density were 16%, 41% and 43%, respectively. Most soybean entries(63%) showed gray pubescence and the remains were brown color. Days from planting to flowering(DPF) showed highly negative correlation with days from flowering to maturity(DFM) and days from planting to maturity (DPM), whereas it was highly positively correlated with leaf area. Days from flowering to maturity was highly positively associated with days from planting to maturity, while it showed highly negatively correlated with leaf area. Days from planting to maturity was highly negative correlation with leaf area.