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- 저자 : JeKal S.J. (검색결과 3 건)
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장선일,김형진,황기명,권태오,제갈승주,김윤철,배현옥,최병민,심재석,곽준수,정헌택,윤용갑,Jang S.I.,Kim H.J.,Hwang K.M.,Kwon T.O.,JeKal S.J.,Kim Y.C.,Pae H.O.,Choi B.M.,Shim J.S.,Kwak J.S.,Chung H.T.,Yun Y.G. 대한한의학방제학회 2002 大韓韓醫學方劑學會誌 Vol.10 No.2
The inhibitory effects of Injinseulki including extracts of melanian snail plus phytomedicinal plants on $CCl_4$-induced fibrosis, $IFN-{\gamma}$ plus LPS-induced production of NO, PMA-stimulated production $O_2$, and tacrine-induced hepatic injury were investigated in rats, RAW 264.7 or HepG2 cells. The ethanol extracts of melanian snail significantly inhibited tarcrine-induced inury of HepG2 cells. Injinseulki inhibited the production of NO and $O_2$ in a dose-dependent manner in activated RAW 264.7 macrophages. Injinseulki significantly inhibited chemical-induced fibrosis in Rats. These results show that Injinseulki including extracts of melanian snail plus phytomedicinal plants may explain some known biological activities of Injinseulki including their anti-inflammatory and anti-fibrotic effect, and is of considerable benefit in the treatment for live diseases.
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제갈승주 ( S J Jekal ) 대한임상검사과학회 1993 대한임상검사과학회지(KJCLS) Vol.25 No.1
With increasing interest being shown in nucleolar organizer region(NORs) in pathology, it was considered of great importance to evaluate the effect of various conditons on the staining properties. NORs can be demonstrated in paraffin sections by a silver technique(AgNOR method) which was developed from a method used by cytogeneticists for the demonstration of NORs in chromosome spreads. The degree of staining is dependent on the staining conditons including fixation regime employed and results may vary greatly. To evaluate the stainability of AgNORs according to the fixation regime, silver concentration, staining temperature and staining time, the author have done AgNORs staining in the normal liver tissue. Twenty Sprague-Dawley adult female rat were divided into four group; 10% neutral buffered formalin, formal-calcium, Carnoy and Bouin. the rats were sacrified, and the liver removed, fixed by immersion, and processed for light microscopy. The fixative schadules and post-treatments used in this study were based on standard sequences from literature and other staining conditions were determined in our option. We have shown that, m general, formalinbased fixatives(lO% neutral buffered formalin and 10% formal-calcium) give optimal results. Although it badly revealed morphological aspect, Carnoy and Bouin fixative were similar to formalin-based fixation in the AgNORs counting. But, Carnoy fixation have shown significant differences in mean AgNOR count per nucleus when compaired with the other groups(p <0.01). We have shown also that it give adequate results at 20%~30%, 20 OC and 40~ 60 min in silver concentration, staining temperature and staining time, respectively.
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