인삼을 천연배지로 하는 고등균류 배양에 관한 연구

鄭宰顯,崔元碩,曺柱鉉 충주대 2004 産業科學論文集 Vol.12 No.-

This research to develop a functional food by using higher fungi from white ginseng, investigated the higher fungi using with ginseng media, the optimal culture conditions of the solid culture and the consistent of solid-product cultured. The results obtained were as follow; 1. Hericium erinaceum, Phellinus linteus and Ganoderma lucidum were observable the growth which is most excellent among 14 strains of the higher fungi using substrate with ginseng residue extract, therefore, it were used for the breed strain. 2. The optimal culture conditions of the solid culture for mycelial growth of Hericium erinaceum, Phellinus linteus and Ganoderma lucidum were added 10%(w/w) of nutrient material as brown rice etc. supplement for maximum mycelial growth. 3. The Ginseng-H. erinaceum (Ginseng-P. linteus, Ginseng-G. lucidum) solid-product cultured under these conditions consisted that were moisture of 3.5%(3.5, 3.6), crude proteins of 7.65%(9.22, 13.57), crude lipid of 8.79%(4.66, 12.02), ash of 9.86%(8.27, 13.38) and carbohydrate of 70.246%(74.33, 57.43).

가토에서 경정맥 이식을 이용한 요도결손 재건술

정재호,이상원,설정현,김효헌 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.5

There are several methods for the reconstruction of partial urethral defect, including split-thickness skin graft, full-thickness skin graft, and preputial flap and free flap. Despite partial success using these methods, most results are unsatisfactory due to frequent complications such as postoperative infection, fistula formation, hair growth and stricture. There have been several pioneering experiments in this field using autogenous vein graft and the results have been controversial. However, the fact that the endothelial lining is replaced by natural urethral epithlium is generally accepted as a positive effect of this method. This experiment was designed to identify the possibility of using vein graft for the reconstruction of partial urethral defect. Two different types of vein graft method were performed in a total of 20 New Zealand White rabbits with partial urethral defect. Firth, for the patch-graft group, a 1×1 cm rectangular vein graft was sutured at a defective area of the same size. Second, for the tubed-graft group, a vein graft segment 1 cm long was replaced at a segmental urethral defect of the same length. Histologic study was performed at three and eight weeks postoperatively in each group. The process of transitional ell epithelial replacement within the grafted vein was uniformly observed in both experimental groups at three weeks postoperatively. At eight weeks postoperatively, the epithelial replacement was almost complete and histologically undistinguishable. In the retrograde urethrogram performed after 8 weeks, the urinary flow in the patch-graft group was normal and showed no stricture, and two of five rabbits in the tubed-graft group showed partial stricture at the graft site. In conclusion, natural urethral epithelium was restored in grafted venous segments irrespective of the type of graft. Partial stricture was observed in 40% of the tubed-graft group while complete reconstruction was possible in the patch-graft group.

젖산균발효두유의 기호성

鄭宰顯 충주대학교 1998 한국교통대학교 논문집 Vol.33 No.2

Soy milks have been compared with the fermented soymilk with lactic acid bacteria to determine if a flavor improvement has been achieved. The fermented soymilks had a satisfactory gelatinous curd an acceptable flavor and smooth texture. The fermented soymilks were virtually devoid of objectionable flavor.

Aspergillus oryzae sp.가 생산하는 amylase에 관한 연구

鄭宰顯 충주대 산업과학기술연구소 1999 産業科學論文集 Vol.7 No.2

These experiments were performed to examined the conditions for the production of amylase of Asp. oryzae sp. on the wheat bran medium and the general properties of amylase. The results obtained were as following : 1.The optimum culture time for the production of amylase on the wheat bran medium was 72 hrs. The optimum adding amount of water for the production of amylase on the wheat bran medium was about 100 %. 2.The optimum pH of amylase was pH 4.5, and the optimum temperture was about 40℃. 3.The additions of Na-oxalate. NaNO2, NaNO3, Na2CO3 and NaHCO3, respectively to the wheat bran medium were effective for the production of amylase. 4.The crude enzyme was activated slightly by the addition of Mg++, Co++, Ba++ and Ca++, but Cu++. Hg++ and Ag++ were inhibited complately.

치과용 디지털 색상 분석용 기기의 정확성과 재현 능력에 대한 평가

정중재,박수정,조현구,황윤찬,오원만,황인남 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.4

본 연구에서는 치과용 디지털 색상 분석기기 중 하나인 ShadeScan (CYNOVAD, Montreal, Canada)의 색상 분석의 정확성과 재현 능력을 평가하였다. 균일한 치아 배열을 가진 20대 성인 남자 8명과 여자 8명의 상악 인상을 고무인 상제 (Exaflex, GC, Japan)를 이용하여 채득하고, 인상에 A2 색상의 임시 크라운 제작용 자가중합형 레진을 주입하여 치아 모델을 완성하고, ShadeScan을 이용해 각 치아의 영상을 얻어 컴퓨터의 ShadeScan 프로그램으로 분석하였다. 그리고 측정 결과의 재현 능력를 평가하기 위해 2개의 치아 모델을 임의로 선택하여 각 치아마다 10회의 색상 측정을 시행하여 분석 결과를 비교하였다. 또한 분석된 영상의 동일 shade 간의 색차를 확인하기 위해 광중합 복합레진인 Gradia Direct (GC, Tokyo, Japan)와 shade guide의 CIE L^(*)a^(*)b^(*) 값을 백색과 흑색 배경판 상에서 spectrophotometer (Spectrolino, GretagMacbeth, USA)로 측정하고, ShadeScan에 의해 동일한 shade로 분석된 shade guide간의 색차를 계산하여 다음의 결과를 얻었다. 모든 치아에서 모델 제작 재료와 동일한 A2 색상므로 분석된 경우는 없었으며, 개개의 치아에서 전체 순면이 동일한 색상으로 분석된 예는 없었다. 또한 동일한 치아군에서 치아의 크기 , 형태 , 순면의 굴곡 등에 따라 서로 다른 색상 분포를 보였다. 재현성 평가에서 각 치아의 중앙부는 비교적 일정한 재현성출 보였으나, 절단부와 주변 부위는 재현성이 낮았으며, 동일한 shade로 분석된 shade guide 간에는 3 이상의 색차 (ΔE^(*))를 보였다. 이상의 결과는 치과용 디지털 색상 분석기기는 비록 높은 재현 능력은 가지고 있지만 정확성에 오류가 있음을 시사하며 , 따라서 이러한 기기를 이용한 색상 분석 시 좀 더 세밀한 주의와 다른 방법의 추가 사용이 필요하리라 사료된다. This study was done to evaluate the reliability of the digital color analysis system (ShadeScan, CYNOVAD, Montreal, Canada) for dentistry. Sixteen tooth models were made by injecting the A2 shade chemical cured resin for temporary crown into the impression acquired from 16 adults. Surfaces of the model teeth were polished with resin polishing cloth. The window of the ShadeScan handpiece was placed on the labial surface of tooth and tooth images were captured, and each tooth shade was analyzed with the ShadeScan software. Captured images were selected in groups, and compared one another. Two models were selected to evaluate repeatability of ShadeScan, and shade analysis was performed 10 times for each tooth. And, to ascertain the color difference of same shade code analyzed by ShadeScan, CIE L^(*)a^(*)b^(*)values of shade guide of Gradia Direct (GC, Tokyo, Japan) were measured on the white and black background using the Spectrolino (GretagMacbeth, USA), and Shade map of each shade guide was captured using the ShadeScan. There were no teeth that were analyzed as A2 shade and unique shade. And shade mapping analyses of the same tooth revealed similar shade and distribution except incisal third. Color difference (ΔE^(*)) among the Shade map which analyzed as same shade by ShadeScan were above 3. Within the limits of this study, digital color analysis instrument for dentistry has relatively high repeatability, but has controversial in accuracy.

혈액투석 환자에게서 발생한 재생불량성 빈혈 1예

정지용,윤나라,강대웅,오종찬,장재현,선길홍,박경희,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Aplastic anemia is a disease characterized by pancytopenia in the peripheral blood and hypocellular bone marrow with the fatty infiltration. The causes of the acquired aplastic anemia were usually related to drugs and immune-related diseases. A 34-years old man was admitted due to general weakness. He has been going hemodialysis for 2 years because of end stage renal disease (ESRD) due to chronic glomerulonephritis. At admission, laboratory tests showed hemoglobin 6.2 g/dL, hematocrit 18.0 %, WBC 5,710mm (neutrophil 71.8%, lymphocyte 19.1%, monocyte 5.9%), reticulocyte 1.9%, platelet 93,000/mm, Fe 12.8 ug/dL, TIBC 204 ug/dL, ferritin 941.47 ng /ml, haptoglobin 0.72 g/L, vitamin B12 508.17 pmol/L, folate 24 ng/mL, total protein 6.54 g/dL, albumin 3.76 g/dL, Alk. pohsphatase 79 IU/L, AST 30.0 IU/L, ALT 39.7 IU/L, total bilirubin 0.57 mg/dl, BUN 79.4 mg/dl and creatinine 10.18 mg/dl. Peripheral blood smear showed the normocytic normochromic anemia with anisocytosis. Anemia was diagnosed but it was irresponsive to the recombinant human erythropoietin (EPO) and blood transfusion. In the bone marrow aspiration smear were there the hypoplastic myeloid and erythroid precursors. The bone marrow biopsy section showed the hypoplasia of all components (10-20%) and the fatty infiltration. We have experienced one case of the idiopathic aplastic anemia in a patient going through hemodialysis and we just report it with documentary records. 저자들은 혈액 투석 중인 환자에서 약물, 면역계 이상등의 원인을 발견할 수 없었음에도 재생 불량성 빈혈이 발생한 1예를 경험하여 문헌 고찰과 함께 보고하는 바이다.

Bacillus polymyxa가 生産하는 β- amylase에 關한 硏究

鄭宰顯 忠州大學校 1988 한국교통대학교 논문집 Vol.22 No.-

These experiments were conducted to investigate the purification and the properties of β-amylase producted by Bicallus polymyxa. The results obtained were as follows : 1. The optimum culture time, initial pH and culture temperature were 100hrs., pH7.0 and 30℃, respectively. 2. The crude enzyme was purified by starch absorption, ammonium sulfate fractionation, DEAE-cellulose column chromatography, CM-cellulose column chromatography and Bio Gel p-150 column chromatography. The specific activity of the purified enzyme was 143u/mg. protein and the yield of enzyme activity was 10.1% 3. The purified enzyme showed a single band on polyacrylamide gel electroporesis. Molecular weight of the purified enzyme was estimated to be approximately On the production conditions of α-amylase from Bacillus subtilis MY-1012. 4. The optimum pH, temperature, the stable pH and temperature range of the purified enzyme were pH 7.0∼8.0, 40℃, pH4.0∼9.0 and below 55℃, respectively.

반응표면 방법에 의한 느타리버섯 (Pleurotus ostreatus) 균사체 생산의 최적화

鄭宰顯 忠州大學校 1996 한국교통대학교 논문집 Vol.31 No.2

For the improvement of mycelial productivity from pleurotus ostreatus through the submerged cultivation, cultural conditions was optimized, using the central composite design with 5 variables and 5 levels, by the response surface methodology computer program. It was shown that the production of mycelia was highly influenced by the concentration of wheat flour, temperature and inoculum size. The interaction between concentration of wheat flour and temperature was higher than other imdependent variables. Stationary points for the production of mycelia was as follows : wheat flour 75.0g/l, nitrogen source(yeast extract : KNO₃(2 : 1))3.0g/l, temperature 30.0℃, pH 6.5, inoculum size 7.5%(v/v).

Bacillus Circulans IAM 1112로부터 Maltotetraose와 Maltopentaose를 생산하는 Amylase의 특성

鄭宰顯 忠州大學校 1994 한국교통대학교 논문집 Vol.29 No.2

The maltooligosaccharide producing amylase was extracted from culture broth of Bacillus circulans IAM 1112 and was puried by ammonium sulfate fractionation and DEAE-cellulose column chromatography. The puried anzyme was homogenous on polyacrylamide gel disc electrop-horesis. The optimum temperature and pH of reaction enzyme were 50℃ and 6.0, respectively pH stability range and themal stability of puried enzyme were pH 5.0-10.0 and stable below 55℃. The enzyme was perfectly inhibited by metal ions such as Hg??,Ag?? and not inhibited Ca??.

Penicillium oxalicum Var.가 生産하는 Glucoamylase에 關한 硏究

鄭宰顯 忠州大學校 1987 한국교통대학교 논문집 Vol.20 No.2

These experiments were conducted to investigate the purification and the properties of glucoamylase produced by Penicilliun oxalicum Var. The results obtained were as follows. 1. Two forms of glucoamylase from Penicillium oxalicum were purified by (NH₄)₂SO₄fractionation, acetone fractionation and succesive column chromatography on DEAE-cellulose, and they were designated glucoamylase Ⅰ(GⅠ) and glucoamylase Ⅱ(GⅡ), respectively. 2. The specific activities of glucoamylase Ⅰ and glucoamylase Ⅱ toward soluble starch were 178 u/mg protein, and 51.5 u/mg protein, and the yields of them were 178 u/mg protein, and 51.5u/mg protein, and the yields of them were 5.8% and 1.8%, respectively. 3. The two purified enzyme were proved to show a single band by polyacrylamide disc gel electrophoresis, and molecular weights of glucoamylase Ⅰ and glucoamylase Ⅱ were estimated to be approximated 84,000 and 86,000 by through Sephadex G-100 Gel filtration, respectively. 4. The optimum pH of glucoamlase Ⅰ and glucoamylase Ⅱ were pH 5.0 and 4.5 respectively. The stable pH ranges of them were pH 3.0-6.5, and optimum temperature of them were 55-60℃ and 60℃, respectively. The stable temperature of them were below 55℃, respectively. 5. Both of the glucoamylase hydrolyzed amylose amylopectin, glycogen and soluble starch, but did not act on isomaltotriose, isomaltose, cellebiose, lactoss, turanose and dextran. 6. Both of the glucoamylase were inhibited by the addition Hg??, Pb??, and the hydrolysis product toward soluble starch of the enzymes were proved to be only glucose by paper chromatography.