http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hyeong-Ju You,Ga-Yeon Kim,Seung-Yeon Kim,Man-Jong Kang 한국동물생명공학회(구 한국동물번식학회) 2021 Journal of Animal Reproduction and Biotechnology Vol.36 No.3
Increasing the efficiency of HR (homologous recombination) is important for a successful knock-in. Rad51 is mainly involved in homologous recombination and is associated with strand invasion. The HR-related mismatch repair system maintains HR fidelity by heteroduplex rejection and repair. Therefore, the purpose of this study is to control Rad51, which plays a critical role in HR, through UV-induced DNA damage. It is also to confirm the effect on the expression of MMR related genes (Msh2, Msh3, Msh6, Mlh1, Pms2) and HR-related genes closely related to HR through treatment with the MMR inhibitor CdCl2. The mRNA expression of Rad51 gene was confirmed in both HC11 cells and mouse testes, but the mRNA expression of Dmc1 gene was confirmed only in mouse testes. The protein expression of Rad51 and Dmc1 gene increased in UV-irradiated HC11 cells. After 72 hours of treatment with 1 μm of CdCl2, the mRNA expression level of Msh3, Pms2, and Rad51 decreased, but the mRNA expression level of Msh6 and Mlh1 increased in HC11 cells. There was no significant difference in Msh2 mRNA expression between CdCl2 untreated-group and the 72 hours treated group. In conclusion, HR-related gene (Rad51) was increased by UV-induced DNA damage. Treatment of the MMR inhibitor CdCl2 in HC11 cells decreased the mRNA expression of Rad51.
You, Hyeong-Ju,Kim, Ga-Yeon,Kim, Seung-Yeon,Kang, Man-Jong The Korean Society of Animal Reproduction and Biot 2021 한국동물생명공학회지 Vol.36 No.3
Increasing the efficiency of HR (homologous recombination) is important for a successful knock-in. Rad51 is mainly involved in homologous recombination and is associated with strand invasion. The HR-related mismatch repair system maintains HR fidelity by heteroduplex rejection and repair. Therefore, the purpose of this study is to control Rad51, which plays a critical role in HR, through UV-induced DNA damage. It is also to confirm the effect on the expression of MMR related genes (Msh2, Msh3, Msh6, Mlh1, Pms2) and HR-related genes closely related to HR through treatment with the MMR inhibitor CdCl<sub>2</sub>. The mRNA expression of Rad51 gene was confirmed in both HC11 cells and mouse testes, but the mRNA expression of Dmc1 gene was confirmed only in mouse testes. The protein expression of Rad51 and Dmc1 gene increased in UV-irradiated HC11 cells. After 72 hours of treatment with 1 ㎛ of CdCl<sub>2</sub>, the mRNA expression level of Msh3, Pms2, and Rad51 decreased, but the mRNA expression level of Msh6 and Mlh1 increased in HC11 cells. There was no significant difference in Msh2 mRNA expression between CdCl<sub>2</sub> untreated-group and the 72 hours treated group. In conclusion, HR-related gene (Rad51) was increased by UV-induced DNA damage. Treatment of the MMR inhibitor CdCl<sub>2</sub> in HC11 cells decreased the mRNA expression of Rad51.
벤조디아제핀과 수면제한이 정신운동기능에 미치는 영향에 대한 예비연구
변준형(Joon-Hyeong Byun),배경열(Kyung-Yeol Bae),진유양(You-Yang Jin),강희주(Hee-Ju Kang),이주연(Ju-Yeon Lee),김성완(Sung-Wan Kim),김재민(Jae-Min Kim),신일선(Il-Seon Shin),김선영(Seon-Young Kim),윤진상(Jin-Sang Yoon) 대한생물치료정신의학회 2014 생물치료정신의학 Vol.20 No.2
Objectives:The use of benzodiazepine and sleep deficiency both impair psychomotor function. Individuals with insufficient sleep often take benzodiazepine during the day for various reasons, such as anxiety. However, few studies have examined the extent to which the psychomotor effects of benzodiazepine interact with insufficient sleep. Therefore, this study examined the interaction of benzodiazepine and sleep deprivation on psychomotor function in healthy young males. Methods:Four healthy males(age 23-26 years) participated in this study. Using a double-blind, placebo-controlled, crossover design, each subject was administered one of the following four conditions in a random order at 1-week intervals : 8 h in bed–placebo ; 8 h in bed–0.5 mg lorazepam ; 4 h in bed–placebo ; or 4 h in bed–0.5 mg lorazepam. Psychomotor functions were assessed at baseline and five times over 8.5 h after taking the study medications using the Critical Flicker Fusion Threshold, Choice Reaction Time, Compensatory Tracking Task, Digit Symbol Substitution Test, and Color-Word Stroop Tests. Results:On the Color Stroop Test, there were significant differences among the conditions at 6.5(T4)(χ2=8.100, p= 0.044) and 8.5(T5)(χ2=8.100, p=0.044) h post-dose. In post hoc tests, 4 h in bed–0.5 mg lorazepam tended to cause more impairment than the other conditions. Conclusion:There was some evidence that benzodiazepine-induced psychomotor impairment is potentiated by sleep deprivation. Future studies with larger sample sizes are required to validate this finding.