원자력 발전소 저압 터빈 동익 자동 초음파 검사 기술 개발

양승한,이정빈,김영호,윤병식,김용식 한국비파괴검사학회 2004 한국비파괴검사학회지 Vol.24 No.4

원자력 발전소의 터빈에서 운전 연수 증가에 따라 pin finger형 블레이드 루트부에 균열이 발생하는 문제점이 발생되었다. 블레이드 루트부에 대한 비파괴검사는 매 계획 예방 정비기간 동안 수동 초음파 검사를 통하여 수행되어 왔으나, 검사의 신뢰성을 확보하기 위해 해당 설비에 대한 자동 초음파 검사 기술 개발의 필요성이 대두되었으며, 이에 따라 자동 초음파 검사 장비 및 검사 기술을 개발하였다. 개발된 검사 장비의 적용성을 확인하기 위해 계획 예방 정비 기간 중 저압터빈 2,3단 블레이드 루트부에 대한 자동 초음파 검사를 수행하고, 다양한 형태의 터빈 검사에 확대 적용 가능성을 확인하였다. As the turbine running duration in nuclear power plants increases, cracks have been found in the pin finger type blade root area. The nondestructive examination for the blade root area has been carried out by manual ultrasonic examination during the overhaul period, but because of necessity to improve the reliability, we developed an automatic ultrasonic examination system and technique. To demonstrate the performance of the developed automatic ultrasonic examination system, low pressure turbine blades in the 2nd and 3rd stages of nuclear power plants were examined using developed system. Its applicability nuclear power plant turbine roots of various types was also confirmed.

수종의 광독성 약물투여 후 시간에 따른 피부농도 및 최소 광독량에 관한 연구

박윤기,조무연,한승경,임성빈 ( Yoon Kee Park,Moo Yon Cho,Seung Kyung Hann,Sung Bin Im ) 대한피부과학회 1991 大韓皮膚科學會誌 Vol.29 No.5

N/A The Skin Concentration and Minimal Phototoxic Dose Following Administration of Phototoxic Drugs as a Function of Time Yoon-Kee Park, M.D., Moo Yon Cho, M.D., Saung Kyung Hann, M.D., Sungbin im, M.D. Department of Dermatology Yonsei Univeraity College of Medicine, Seoul, Korea Psoralen photochemotherapy(PUVA) is defined as a treatment that depends on the interaction of a photosensitizing substance, psoralen, and long wave jltraviolet(UVA) radiation that results in therapeutically beneficial effects. The rationale of its use is to clear skin disease by repeated controlled phototoxic reactions. The efficacy of PUVA depends on the drug, the UVA dose and wave length, the individual sensitivity to the phototoxic reaction and on the disease to be treated. However, the major factor is the amount of psoralen at the site of photochemical reaction in skin. Daspite the extensive experiences which have now accumulated in the use of PUVA, the relationship between the therapeutic response and phototoxicity as it occurs in normal skin is not established. Comparative data on different psoralen molecules and their skin sensitizing ability are required. From studies on the skin concentration using HPLC and minimal phototoxic dose following oral administration, intraperitoneal administration, a bath of phototoxic drugs as a function of time in guinea pigs, the following results are obtained. 1. The skin concentration of phototoxic drugs after oral administration peaked at 1.5 hours, and the concentration of 8-methoxypsoralen(8-MOP) was 3.7 times greater than that of 5-methoxypsoralen(5-MOP). The skin concetration of 4,5,8-trimethylpsoralen(TMP) was not detected in this study(limit of sensitivity 5ng/g). 2. The skin conentration of phototoxic drugs after intraperitoneal administration peaked at 30 minutes, and the concentration of 8-MOP was slightly greater than that of 5-MOP. The skin concentration of TMP was not detected in this study(limit of sensitivity 5ng/g). 3. The skin concentration of phototoxic drugs after bathing peaked immediately after bathing, and the skin concentrations of phototoxic drugs after bathing decreased in the order of 5-MOP, TMP, and 8-MOP. 4. The minimal phototoxic dose of phototoxic drugs after oral administration was lowest at 1.5 hours, and the minimal phototoxic dose of 8-MOP was 8 times less than 5-MOP, 24 times less than TMP. The phototoxicity of 8-Mop after oral administration was strongest. The time of lowest phototoxic dose and highest skin concentration was the same. 5. The minimal phototoxic dose of phototoxic drugs after intraperitoneal administration was lowest at 30 minutes, and the minimal phototoxic dose of 8-MOP was 1.3 times less than 5-MOP, 5.7 times less than TMP. The phototoxicity of 8-MOP after intraperitoneal administration was strongest. The time of lowest phototoxic dose and highest skin concentration was same. 6. The minimal phototoxic dose of phototoxic drugs after bathing was lowest at 15 minutes. The minimal phototoxic dose of 8-MOP was not significantly different but was significantly greater than that of 5-MOP.

최근 5년간 요로감염의 원인균 및 항생제 감수성

김세웅,임필빈,정웅교,이승주,이지열,조용현,윤문수 대한화학요법학회 2002 대한화학요법학회지 Vol.20 No.3

배경 : 최근 요로감염 원인균에 대한 내성 증가로 항생제 감수성에 변화를 가져와 요로감염 환자에게 경험적 1차 치료를 위한 항생제 선택에 어려움을 주고 있다. 단순 및 복잡성 요로감염으로 진단 받은 환자들을 대상으로 원인균과 그에 따른 항생제의 감수성을 살펴봄으로써 향후 단순 및 복잡성 요로감염에 대한 항생제의 선택에 도움을 받고자 한다. 대상 및 방법 : 1996년 6월부터 2001년 5월까지 요배양 검사상 요로 감염균이 10^5cfu/ml 이상의 집락이 형성된 200명을 대상으로 해부학적 또는 구조적인 장애 유무에 따라 단순 요로감염 및 복잡성 요로 감염으로 나누어 각각의 원인균과 그에 따른 항생제의 감수성을 후향적으로 분석하였다. 결과 : 복잡성 요로감염의 가장 흔한 원인질환은 신경인성 방광이었다. 단순 요로감염의 가장 흔한 원인균은 E. coli (61.35%)였고 복잡성 요로감염에서는 E. coli 이외의 균이 E. coli 보다 더 흔한 원인균이었다(58.9%). 단순 및 복잡성 오료감염 중 그람 양성균에서는 vancomycin, penicillin 이 높은 감수성을 보인 반면 ciprofloxacin, imipenem이 낮은 감수성을 보였고 그람음성균에서는 amikacin, imipenem이 높은 감수성을 보인 반면 ampicillin, bactrim이 낮은 감수성을 보였다. 결론 : 단순 및 복잡성 요로 감염의 주 원인균으로 그람 음성균주 특히, Ecoli에 의한 감염이 많은 비중을 차지하고 있으나 빈도가 점차적으로 감소하는 추세이다. 또한 병원성 감염 및 도뇨관의 장기 유치에 따른 교대균의 증가, 비병원성 세균의 출현 등으로 인한 E. coli 이외의 기타 그람음성균 및 그람양성균의 증가하는 추세이다. 향후 임상에서 요로감염의 일치 치료제로 많이 사용되고 있는 ampicillin과 bactrim 등의 항생제의 감수성이 낮으므로 사용시 필요하다고 사료된다. Backround : The increasing frequency of failure of urinary tract infections (UTIs) may be due to emerging resistance to commonly prescribed antimicrobials. We studied the causative microorganisms and their antibiotic sensitivities in uncomplicated and complicated UTIs. Methods : We analyzed 219 microorganisms and their antibiotic sensitivities of 200 outpatients who had more than 10^5cfu/ml on urine from June 1996 to May 2001 retrospectively. The patients weredivided into 2 groups, uncomplicated UTI group and commplicated UTI group according to with or without underlying disease. Results : The most common underlying disease of complicated UTI was neurogenic bladder. The most common pathogenic microorganism was Escherichia coli (E. coli) (61.35%) in uncomplicated UTI group but in complicated UTI, non-E, coli(58.9%) was more common than E. coli. In uncomplicated and complicated UTI, vancomycin, penicillin showed relatively higher sensitivity of gram positive organisms, while ciprofloxacin, imipenem showed relatively lower sensitivity, and imipenem, amikacin showed relatively higher sensitivity of gram negative organisms, while bactrim, amipicillin showed relatively lower sensitivity. Conclusion : We consider that gram negative microorgansisms, especially E. coli, is the main cause of UTI. But, we should concern about the increase of gram positive organsms and other gram negative organisms besides E. coli. Bactrim, amipicllin which were prescribed empirically in UTIs, showed relatively lower sensitivity. Regarding tom the choice of adequate drug in the treatment of UTI, it is necessary to consider the change of pathologic microorganisms.

Synthesis and Evaluation of Succinic Acid Derivatives as Prolyl Endopeptidase Inhibitors

( Seung Bin Yoon ),( Jae Sung Yang ),( Kyung Ho Lee ),( Hyeon Gyu Han ),( Ah Reum Han ),( Young Sook Paik ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.5

Many synthetic peptide-based prolyl endopeptidase (PEP) inhibitors are depended on the acyl-Lprolyl-pyrrolidine structure. The pyrrolidine ring at P1 and a large variety of lipophilic acyl groups at P3 have been reported to be important for potent PEP inhibitory activity. Upon exploration of a new lead compound for PEP inhibition, succinic acid was introduced at the P2 position to replace the L-proline in acyl-L-prolyl-pyrrolidine. A series of amido-succinylpyrrolidine compounds (1-9) were synthesized, and their PEP inhibitory activities were evaluated. Of these, compound 9 showed the most potent PEP inhibitory activity with IC50 of 6.4±0.6 nM.

Synthesis and Evaluation of Succinic Acid Derivatives as Prolyl Endopeptidase Inhibitors

Seung-Bin Yoon,Jae-Sung Yang,Kyung-Ho Lee,Hyeon-Gyu Han,Ah-Reum Han,Young-Sook Paik 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.5

Many synthetic peptide-based prolyl endopeptidase (PEP) inhibitors are depended on the acyl-Lprolyl-pyrrolidine structure. The pyrrolidine ring at P1 and a large variety of lipophilic acyl groups at P3 have been reported to be important for potent PEP inhibitory activity. Upon exploration of a new lead compound for PEP inhibition, succinic acid was introduced at the P2 position to replace the L-proline in acyl-L-prolyl-pyrrolidine. A series of amido-succinylpyrrolidine compounds (1-9) were synthesized, and their PEP inhibitory activities were evaluated. Of these, compound 9 showed the most potent PEP inhibitory activity with IC50 of 6.4±0.6 nM. Many synthetic peptide-based prolyl endopeptidase (PEP) inhibitors are depended on the acyl-Lprolyl-pyrrolidine structure. The pyrrolidine ring at P1 and a large variety of lipophilic acyl groups at P3 have been reported to be important for potent PEP inhibitory activity. Upon exploration of a new lead compound for PEP inhibition, succinic acid was introduced at the P2 position to replace the L-proline in acyl-L-prolyl-pyrrolidine. A series of amido-succinylpyrrolidine compounds (1-9) were synthesized, and their PEP inhibitory activities were evaluated. Of these, compound 9 showed the most potent PEP inhibitory activity with IC50 of 6.4±0.6 nM.

Developmental competence of bovine early embryos depends on the coupled response between oxidative and endoplasmic reticulum stress.

Yoon, Seung-Bin,Choi, Seon-A,Sim, Bo-Woong,Kim, Ji-Su,Mun, Seong-Eun,Jeong, Pil-Soo,Yang, Hae-Jun,Lee, Youngjeon,Park, Young-Ho,Song, Bong-Seok,Kim, Young-Hyun,Jeong, Kang-Jin,Huh, Jae-Won,Lee, Sang-R Society for the Study of Reproduction [etc.] 2014 BIOLOGY OF REPRODUCTION Vol.90 No.5

<P>The stress produced by the coupling of reactive oxygen species (ROS) and endoplasmic reticulum (ER) has been explored extensively, but little is known regarding their roles in the early development of mammalian embryos. Here, we demonstrated that the early development of in vitro-produced (IVP) bovine embryos was governed by the cooperative action between ROS and ER stress. Compared with the tension produced by 5% O2, 20% O2 significantly decreased the blastocyst formation rate and cell survival, which was accompanied by increases in ROS and in levels of sXBP-1 transcript, which is an ER stress indicator. In addition, treatment with glutathione (GSH), a ROS scavenger, decreased ROS levels, which resulted in increased blastocyst formation and cell survival rates. Importantly, levels of sXBP-1 and ER stress-associated transcripts were reduced by GSH treatment in developing bovine embryos. Consistent with this observation, tauroursodeoxycholate (TUDCA), an ER stress inhibitor, improved blastocyst developmental rate, trophectoderm proportion, and cell survival. Moreover, ROS and sXBP-1 transcript levels were markedly decreased by supplementation with TUDCA, suggesting a possible mechanism governing the mutual regulation between ROS and ER stress. Interestingly, knockdown of XBP-1 transcripts resulted in both elevation of ROS and decrease of antioxidant transcripts, which ultimately reduced in vitro developmental competence of bovine embryos. Based on these results, in vitro developmental competence of IVP bovine embryos was highly dependent on the coupled response between oxidative and ER stresses. These results increase our understanding of the mechanism(s) governing early embryonic development and may improve strategies for the generation of IVP embryos with high developmental competence.</P>

Record Effective Mobility Obtained From In_0.53Ga_0.47As/In_0.52Al_0.48As Quantum-Well MOSFETs on 300-mm Si Substrate

Seung-Woo Son,Jung Ho Park,Ji-Min Baek,Do-Kwyn Kim,Seung Ryul Lee,Sang-Moon Lee,Jieon Yoon,Jungtaek Kim,Woo-Bin Song,Sunjung Kim,Dong Suk Shin,Yihwan Kim,Seung Heon Shin,Tae-Woo Kim,Jung-Hee Lee,Dae-H IEEE 2017 IEEE electron device letters Vol.38 No.6

<P>In this letter, we have investigated the properties of In0.53Ga0.47As quantum-well (QW) metal-oxide-semiconductor field-effect-transistors (MOSFETs) on a 300-mm(100) Si wafer. We have explored the impact of scaling down In0.53Ga0.47As channel thickness (t(ch)) from 15 to 5 nm. The fabricated devices show excellent electrostatic integrity, such as subthreshold-swing (SS) < 80 mV/decade and drain-induced barrier-lower (DIBL) < 20 mV/V. Furthermore, we have extracted the effective mobility (mu(n_eff)) of the fabricated devices to investigate the carrier transport properties of the InGaAs MOSFETs with different values of t(ch). The device with t(ch) = 15 nm displayed a value of mu(n_eff) = 2, 190 cm(2)/V-s at room temperature. This value was found to decrease as tch was scaled down.</P>

Primate resource infrastructure for biomedical and basic science

Seung-Bin Yoon,Younghoon Son,Dong-Ho Lee,Ja-Rang Lee,Sangil Lee,Ki Jin Kim,Sang-Yong Lee,Se-Yong Kim,Taeho Kwon,Min Seong Kim,Byoung-Jin Park,Jun-Ki Lee,Yu-Jin Jo,Ji-Su Kim 한국실험동물학회 2020 한국실험동물학회 학술발표대회 논문집 Vol.2020 No.7

Effective superovulation method according to weight and age of cynomolgus monkey (Macaca fascicularis)

Seung-Bin Yoon,Sim Bo-Woong,Jeong Kang-Jin,Yeonghoon Son,Ja-Rang Lee,Dong-Ho Lee,Sang-Il Lee,Ki Jin Kim,Ji-Su Kim,Sun-Uk Kim 한국실험동물학회 2018 한국실험동물학회 학술발표대회 논문집 Vol.2018 No.7

Synthesis and Evaluation of Succinic Acid Derivatives as Prolyl Endopeptidase Inhibitors

Yoon, Seung-Bin,Yang, Jae-Sung,Lee, Kyung-Ho,Han, Hyeon-Gyu,Han, Ah-Reum,Paik, Young-Sook The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.5

Many synthetic peptide-based prolyl endopeptidase (PEP) inhibitors are depended on the acyl-L-prolyl-pyrrolidine structure. The pyrrolidine ring at P1 and a large variety of lipophilic acyl groups at P3 have been reported to be important for potent PEP inhibitory activity. Upon exploration of a new lead compound for PEP inhibition, succinic acid was introduced at the P2 position to replace the L-proline in acyl-L-prolyl-pyrrolidine. A series of amido-succinyl-pyrrolidine compounds (1-9) were synthesized, and their PEP inhibitory activities were evaluated. Of these, compound 9 showed the most potent PEP inhibitory activity with $IC_{50}$ of 6.4${\pm}$0.6 nM.