韓國人에서 重合酵素反應으로 檢索되는 COL2A1 遺傳座位의 對立遺傳子 頻度

黃迪駿,明賢君,李羲碩,郭明宰 大韓法醫學會 1994 대한법의학회지 Vol.18 No.2

With the human genomic DNAs obtained from 128 unrelated Korean, the VNTR region 3' to the collagen type Ⅱ gene(COL2A1) was amplified in vitro by the polymerase chain reaction(PCR), and separated by 3% agarose(2% Nusieve and 1% Seakem) and 4% polyacrylamide gel' and analyzed to get the population genetic data for COL2A1 VNTR allele such as allele and genotype frequencies, and hetrozygosity were calculated based on the amplified fragments length polymorphism(AMP-FLP). 1. In 128 unrdlated Korean, 4 allelic variants(K2, K3, K4, and K5) at COL2A1 VNTR locus are observed, but K1 allele which was also observed in other study was not. 2. The observed allele frequencies for K4, K5, k3 and k2 are 0.519, 0.383, 0.094 and 0.008, respectively. 3. Of 10 possible genotypes, only 4 heterozygous genotypes(K5/K4, K5/K3, k4/K3, K4/K2) and 2 homozygous genotypes(K5/K5, K4/K4) are observed. However, the observed numgers of homozygous and heterozygous genotypes(46 and 82, respectively) are not significatly different from their expectations, 53.3 and 74.7. 4. The observed genotype frequencies are ordered as K5/K4(0.445), K4/K4(0,242), K5/K5(0.117), K5/K3(0.086) and K4/K2(0.086), which are in accordance with the Hardy-weinberg prediction based on the observed allele frequencies. 5. The overall heterozygosity at this locus is 0.563, while its expectation based on the allele frequencies is 0.584

Application of an IPM-based Spray Program to Protected Cultivation of Strawberry in Korea

Myeong Hyeon Nam,Hyeon Suk Kim,Won Keun Lee,Yeol Kyu Seong,Mark. L. Gleason,Jeong Young Song,Hong Gi Kim 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.5

An IPM-based spray program (ISP), based on ecological and agronomic information, is necessary for reducing pesticide sprays and producing strawberries safely. In this study, the applicability of ISP to safe production of strawberries was compared with a conventional spray program (Con-SP) during the 2005-2006 and 2006-2007 seasons in plastic houses in Nonsan, Korea. The ISP program consisted of fungicide, insecticide, and miticide treatments as well as release of natural enemies before and after harvest. The ISP treatment schedule was as follows: dipping transplants in azoxystrobin before transplanting (mid-September); spraying with abamectin and thiacloprid when black mulch was applied (early October); spraying with pyraclostrobin + acetamiprid and tetraconazole + abamectin before blooming in early and mid-November, respectively; transplanting banker plants colonized by Aphidius colemani in mid-February (2005-2006 season) or November (2006-2007 season); and releasing Phytoseiulus persimilis in mid-March. Number of pesticide sprays per season for ISP and Con-SP was five and nine, respectively. Anthracnose crown rot, caused by Colletotrichum gloeosporioides, did not occur in ISP in either season, but incidence was ≤ 3.3% in Con-SP during the 2005-2006 season. Powdery mildew (Sphaerotheca aphanis var. aphanis) did not occur during harvest in ISP, but occurred during early and late harvest stages in Con-SP in both years. Incidence of Botrytis fruit rot did not differ between ISP and Con-SP. Two-spotted spider mite (Tetranychus urticae) did not appear until March in ISP; subsequently, spider mite density remained at ≤-0.5 mite per leaf until the last harvest period in both years. The number of spider mites in ISP and Con-SP during the harvest did not differ. Populations of cotton aphids (Aphis gossypii) were also higher in ISP than Con-SP but decreased from March to April, accompanied by production of aphid mummies by A. colemani. The results showed that ISP was more effective than Con-SP, and required fewer pesticide sprays.

Effect of Fungicides in the Control of Anthracnose of Strawberry Plants Caused by Colletotrichum gloeosporioides

Myeong Hyeon Nam,Hyeon Suk Kim,In Ha Lee,Won Keun Lee,Yeol Kyu Seong,Natalia Peres 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

쯔쯔가무시병에 동반된 간질성 폐렴에 관한 연구

이현숙(Hyeon Suk Lee),김대수(Dae Soo Kim),진영주(Young Joo Chin),김성진(Seong Jin Kim),송형근(Hyeong Keon Song),성노현(No Hyeon Seong),이상도(Sang Do Lee),김명수(Myeong Su Kim) 대한내과학회 1996 대한내과학회지 Vol.50 No.1

N/A Objectives: Interstitial penumonia is a major cause of death in scrub typhus but little is known about its pathology and pathogenesis. To study the clinical findings, pathology and pathogenesis of interstitial pneumonia in scrub typhus, we performed this study. Methods: We performed clinical evaluation, laboratory tests, radiological evaluation including high resolution CT and pulmonary function tests in 53 scrub typhus patients. Bronchoalveolar lavage(BAL) and transbronchial lung biopsy(TBLB) were done in patients with interstitial pneumonia and electron microscopic examination and immunofluorescence study including monoclonal antibody against Rickettsia tsutsugamushi were done. Results: 1) Interstitial pneumonia was found in 13(26%) patients. 2) Radiological findings: High-resolution CT showed diffuse ground glass opacity, thickening of bronchovascular bundie and prominent central core of secondary pulmonary lobule with minimal thickening of interlobular septum and there were no findings suggesting vasculitis 3) Cells in HAL fluid: total cell count, (46±9)×104/ml, alveolar macrophage, 68±17%, lymphocyte, 28±15% and neutrophil, 5±3% Pathologic finding(TBLH): 4) Light microscope: interstitial edema with mononuclear cell infiltration, mainly lymphocyte, without any evidence of vasculitis or perivasculitis 5) Immunofluorescence and eletron microscope ' IF study, using anti-Ig G,A,M, anti-Cg, anti-fibrinogen and anti-rickettsial Ab, revealed negative reaction. Electron photomicrograph revealed no rickettsial organism. Conclusion: In interstital pneumonia associated with scrub typhus we noted lymphocytosis in BAL fluid and interstitial inflammation in histopathology without any evidence of vasculitis. Thus vasculitis is not the only main pathology but other mechanism including immunopathogenesis may operate in the pathogenesis of intersititial pneumonia in scrub typhus

살균제와 미생물제 처리시기에 따른 딸기 잿빛곰팡이병 방제효과

남명현(Myeong Hyeon Nam),김현숙(Hyeon Suk Kim),이원근(Won Keun Lee),Mark L. Gleason,김홍기(Hong Gi Kim) 한국원예학회 2011 원예과학기술지 Vol.29 No.2

Botrytis cinerea에 의한 딸기 잿빛곰팡이병은 최대 50%까지 발생하여 큰 피해를 주는 딸기의 주요 병해이다. 약제 살포시기는 잿빛곰팡이병의 성공적인 방제를 좌우하는 필수적 요소이다. 딸기 ‘설향’ 품종에 대한 잿빛곰팡이병 방제효과 검정을 위해 fenhexamid + imonoctadine tris, cyprodinil + fludioxonil, Bacillus subtilis QST713을 ex vivo에서 개화전, 개화기, 미착색과, 성숙과 단계부터 처리하여 효과를 비교하였다. 또한 ‘설향’ 품종을 공시하여 ex vivo와 재배포장에서 cyprodinil + fludioxonil 혹은 fludioxonil을 저온(0℃, 5시간) 전후 처리하여 잿빛곰팡이병 방제효과를 조사하였다. 딸기 꽃과 성숙과실은 미착색과실보다 B. cinerea에 더 감수성이었다. ex vivo에서 개화단계별 약제방제효과는 fenhexamid + iminoctadine tris는 개화직전과 개화기, cyprodinil + fludioxonil은 개화기에 효과적이었으나 Bacillus subtilis QST713은 처리간 유의성이 없었다. 2010년 딸기 잿빛곰팡이병 방제효과는 fludioxonil을 개화기에 1주일 간격으로 2회 처리시 효과적이었다. 딸기 ‘Redpearl’과 ‘설향’ 품종은 ‘매향’과 ‘Akihime’ 품종보다 저온에 더 감수성이어서 주의를 요했으며 cyprodinil + fludioxonil을 저온처리 전에 살포시 90%이상의 방제효과를 나타냈다. 2010년 재배포장에서 잿빛곰팡이병 방제효과는 저온 발생 전 혹은 저온 발생 후 1주일 간격 2-3회 fludioxonil처리가 효과적이었다. 이런 결과로 딸기잿빛곰팡이병 방제를 위한 약제의 효과적인 처리시기는 개화와 저온이 밀접한 관련이 있음을 알 수 있었다. The fungus Botrytis cinerea causes fruit rot of strawberry and the damages can result in harvest losses upto 50%. Proper timing of fungicide application is essential for successful control of Botrytis fruit rot, fenhexamid plus iminoctadine tris, cyprodinil plus fludioxonil, fludioxonil alone, and Bacillus subtilis QST713 were applied to individual buds, flowers, and green and red fruit of cultivar ‘Seolhyang’ ex vivo. Cyprodinil plus fludioxonil or fludioxonil alone was applied i) before and after a 5-hr period of low-temperature (0℃) incubation ex vivo ii) in field trials. Strawberry flowers and red fruit were more susceptible to B. cinerea than the green fruits. Incidence of Botrytis rot with fenhexamid plus iminoctadine tris and cyprodinil plus fludioxonil was the lowest at flowering, whereas B. subtilis QST713 did not significantly among treatments. In 2010, incidence of Botrytis fruit rot was significantly reduced when fludioxonil was applied two times at 1 week intervals from 50% bloom in field trials. Cultivars Redpearl and Seolhyang were more susceptible to low-temperature than cvs. Maehyang and Akihime. Cyprodinil plus fludioxonil application was effective when applied before onset of the low-temperature treatment period. Fludioxonil showed the most effective when it was sprayed one and more than two times in before and post low-temperature condition, respectively. These results demonstrate that fungicide selection and timing can interact with stage of fruit development and low-temperature in determining effectiveness of suppression of Botrytis fruit rot.

Physicochemical properties of fish-meat gel from Paralichthys olivaceus

Myeong Sook KWON,Ya GAO,Jung Hwan OH,Ga Hyun YU,Se Jong KIM,Hyung Kwang KIM,Jun Mo JUNG,Ji Hyeon CHEON,Chang-Suk KONG 한국생물공학회 2016 한국생물공학회 학술대회 Vol.2016 No.4

Laparoscopic primary repair of gastric perforation caused by umbilical trocar insertion

( Hyeon Myeong Hong ),( Joong Sub Choi ),( Jae Man Bae ),( Won Moo Lee ),( Un Suk Jung ),( Jeong Min Eom ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-

Objective: Present an unusual case Target: Adanced practicioners Methods: Background and Aims: We present a case of iatrogenic gastric perforation caused by umbilical trocar insertion with subsequent laparoscopic primary repair. Design: Case study. Patients: A 58-year-old Korean woman with no history of abdominal surgery was scheduled to undergo a laparoscopic assisted vaginal hysterectomy for myoma ureti. Interventions: Laparoscopy. Results: A 58-year-old Korean woman had a laparoscopic assisted vaginal hysterectomy on June 26, 2019. During abdominal entry, a 5-mm trocar was then placed by direct trocar insertion method, but introduction of the laparoscope revealed that it had been placed within the lumen of the stomach. We inserted nasogastric tube to remove gas and liquid from the stomach. The stomach perforation, which had been marked by leaving the original trocar in place and appeared to be an 5-mm clean injury with minimal bleeding The perforation site was repaired laparoscopically with 2-0 vicryl sutures, using intracorporeal interrupted full-thickness suture. Her nasogastric tube was removed on postoperative day one. The patient did well postoperatively and recovered without complications. Conclusion: Our experience indicate that laparoscopic primary repair of gastric perforation caused by umbilical trocar insertion is safe and feasible.

Successful repair of rectal perforation during the laparoscopic sacrocolpopexy in patient of vaginal vault prolapse

( Hyeon Myeong Hong ),( Yeon Kyoung Kim ),( Jeong Min Eom ),( Joong Sub Choi ),( Jaeman Bae ),( Won Moo Lee ),( Un Suk Jung ) 대한산부인과학회 2021 대한산부인과학회 학술대회 Vol.107 No.-

The Effects of Hijikia fusiforme on the Skin Regeneration and Antioxidant Activities

Min-Suk Kang,Bo-Kyung Kim,Myeong-Jeong Jeon,Mihyang Kim,Sang-Hyeon Lee 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10

Improved PCR for identification of Pseudomonas aeruginosa

Choi, Hyeon Jin,Kim, Myeong Ho,Cho, Min Seok,Kim, Byoung Kyu,Kim, Joo Young,Kim, ChangKug,Park, Dong Suk Springer-Verlag 2013 Applied microbiology and biotechnology Vol.97 No.8

<P>The aim of the present study was to develop a noble and specific marker for a quantitative polymerase chain reaction (PCR) assay for the species-specific detection of Pseudomonas aeruginosa based on the O-antigen acetylase gene. It is an important challenge to characterize populations of the bacterium P. aeruginosa, an opportunist by virtue of its physiological and genetic adaptability. However, molecular and serological methods currently available for sensitive and specific detection of P. aeruginosa are by no means satisfactory because there have been critical defects in the diagnosis and identification of P. aeruginosa strains in that these assays also detect other Pseudomonas species, or do not obtain amplified products from P. aeruginosa strains. Therefore, a primer set was designed based on the O-antigen acetylase gene of P. aeruginosa PA01 because it has been known that this gene is structurally diverse among species. The specificity of the primer set was evaluated using genomic DNA from six isolates of P. aeruginosa, 18 different species of Pseudomonas, and 23 other reference pathogenic bacteria. The primer set used in the PCR assay amplified a 232-bp amplicon for only six P. aeruginosa strains. The assay was also able to detect at least 1.41??10(3)?copies/μl of cloned amplified target DNA using purified DNA, or 2.7??10(2) colony-forming unit per reaction when using calibrated cell suspension. In conclusion, this assay can be applied as a practical diagnostic method for epidemiological research and the sanitary management of water with a low level or latent infection of P. aeruginosa.</P>