Candidate Genes with Ovulation by Differential Display PCR in Small Tail Han Sheep

Liu, Shufang,Li, Hongbin,Song, Xuemei,Wang, Aihua,Wei, Caihong,Du, Lixin Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.9

To find the candidate genes concerned with ovulation rate of sheep, Differential Display Reverse Transcription Polymerase Chain Reaction was employed to find the differently expressed cDNA controlling ovulation in the Small Tail Han sheep of polyembryony and in Tan sheep of single birth. Twenty-four primer pairs of three anchored primers and eight arbitrary primers were assembled to amplify the specialized bands from these sheep. Positive cross tests were applied to optimize the ascertainable PCR conditions in which different special bands can be identified by silver strain in one PCR tube. After eliminating the false positive PCR products by Northern hybridization, 24 differential display bands were acquired from the ovary in the Small Tail Han sheep. These EST bands were sequenced and 18 different ESTs were found in which five ESTs had several copies and 13 ESTs had only one copy. Comparing these ESTs with homologous sequences by BLAST in the GenBank, there were six ESTs with known open reading frame (ORF) and function, three ESTs with known ORF and no function, and 9 ESTs without homologous sequence. These ESTs partly represent several genes such as NOS2, tensin, TCRA, CDKN1A, ESR1 and ACTB which express especially in Small Tail Han sheep.

유도탄 발사 초기 구조건전성 평가를 위한 공압시험

권혁민(Hyeokmin Kwon),장홍빈(Hongbin Jang),윤지수(Jisu Yoon),구송회(Song-Hoe Koo) 한국추진공학회 2018 한국추진공학회 학술대회논문집 Vol.2018 No.12

유도탄 발사 초기 발사관 내압이 발달하는 상황에서의 유도탄 구성품의 구조건전성 평가를 위해 공압시험을 수행하였다. 노즐마개의 파열은 상용 파열판을 이용해 모사하였다. 파열판의 파열 압력은 전산유체해석을 통해 목표하는 발사관 후방 압력을 형성하기에 충분한 값으로 설정하였다. 총 2회의 시험을 수행하였으며, 그 결과를 통해 유도탄 구성품의 구조건전성을 평가하였다. Cold-gas Test is conducted to evaluate structural integrity of missile components at the initial phase of the missile launch, in which canister internal pressure develops. The removal of a nozzle closure is simulated using a commercial rupture disk. Through computational fluid dynamic analysis, rupture pressure of the disk is set to a value sufficient to form target canister rear end pressure. The test is conducted twice, and the structural integrity of the missile components is evaluated using these test results.

Sintering technology and properties of sponge city permeable bricks prepared from electrical porcelain was

Yue Liu,Yun Xiang,Jieguang Song,Dongliang Zhang,Hongbin Wen,Yuzhen Wang 한양대학교 청정에너지연구소 2022 Journal of Ceramic Processing Research Vol.23 No.6

The generated electric porcelain waste increases with the rapid development of the electric porcelain industry. Unreasonableutilization results in the waste of resources and environmental pollution. However, at present, many coal gangues and fly ashesare wasted and underutilized in my country. In this paper, electric porcelain waste is used as the main raw material, and itssintering temperature, holding time, and heating rate are discussed to study the performance of the permeable brick preparedfrom electric porcelain waste. Results show that with the increase in sintering temperature, the porosity and waterpermeability coefficient of permeable bricks decrease, and their volume shrinkage rate and compressive strength show anincreasing trend. Furthermore, porosity decreases with the increase in holding time. The volume shrinkage rate andcompressive strength increase, and the water permeability coefficient decreases. The volume shrinkage rate of the permeablebrick decreases, and its porosity, water permeability coefficient, and compressive strength increase with the heating rate. Combined with the comprehensive consideration of the porosity, volume shrinkage rate, water permeability coefficient, andcompressive strength of permeable bricks, the best sintering process for preparing permeable bricks from electrical porcelainwaste is presented with a sintering temperature of 1225 °C, holding time of 1 h, and heating rate of 6 °C/min. The porosityof the prepared permeable brick is 79.5%, the volume shrinkage rate is 25.79%, the water permeability coefficient is 2.82×10-2cm/s, and the compressive strength is 5.79 MPa.

Interaction Between Serum/Glucocorticoid-Regulated Kinase 1 and Interleukin-6 in Chronic Rhinosinusitis

Lai Yuting,Hu Li,Yang Lu,Hu Xianting,Song Xiaole,Yang Jingyi,Li Hongbin,Chen Kun,Li Huabin,Wang Dehui 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.5

Purpose: Serum/glucocorticoid-regulated kinase 1 (SGK1) has recently emerged as a critical regulator of inflammatory diseases. In this study, we examined SGK1 expression and its possible pathogenic roles in chronic rhinosinusitis (CRS). Methods: Immunohistochemistry, western blotting, Bio-Plex assay, enzyme-linked immunosorbent assays, and quantitative real-time polymerase chain reaction were performed to assess protein and gene expression levels. The mRNA expression levels of SGK1 and interleukin-6 (IL-6) were extracted from a CRS database to perform correlation analysis. Stable cell lines with SGK1 overexpression (16HBE) and knockdown (A549) were constructed to investigate the interaction between SGK1 and IL-6 in vitro. Results: SGK1 exhibited strong cytoplasmic and nuclear staining in the epithelial layers and the lamina propria of nasal polyps (NPs) and in the mucosal tissues of CRS without nasal polyps (CRSsNP). The mRNA and protein expression levels of SGK1 and IL-6 were significantly increased in NPs and CRSsNP tissues, compared to control tissues. SGK1 phosphorylation was significantly greater in NPs than in CRSsNP tissues (P < 0.01). The mRNA levels of SGK1 and IL-6 were significantly correlated (P < 0.001, r = 0.649). Exposure to IL-6 significantly increased SGK1 expression in cultured dispersed NP cells, 16HBE cells, and A549 cells. IL-6 expression was significantly down-regulated in SGK1-overexpressing 16HBE cells (P < 0.01) and significantly up-regulated in SGK1-knockdown A549 cells (P < 0.05). Administration of GSK650394, a SGK1 inhibitor, significantly increased IL-6 self-induced mRNA expression in cultured dispersed NP cells and 16HBE cells. Conclusions: The interaction between SGK1 and IL-6 may play an anti-inflammatory role in IL-6-induced inflammation in the pathogenesis of CRS.

Parallel Gene Loss and Acquisition Among Strains of Different Brucella Species and Biovars

Zhijun Zhong,Yufei Wang,Jie Xu,Yanfen Chen,Yuehua Ke,Xiaoyan Zhou,Xitong Yuan,Dongsheng Zhou,Yi Yang,Ruifu Yang,Guangneng Peng,Hai Jiang,Jing Yuan,Hongbin Song,Buyun Cui,Liuyu Huang,Zeliang Chen 한국미생물학회 2012 The journal of microbiology Vol.50 No.4

The genus Brucella is divided into six species; of these, B. melitensis and B. abortus are pathogenic to humans, and B. ovis and B. neotomae are nonpathogenic to humans. The definition of gene loss and acquisition is essential for understanding Brucella’s ecology, evolutionary history, and host relationships. A DNA microarray containing unique genes of B. melitensis Type strain 16MT and B. abortus 9-941 was constructed and used to determine the gene contents of the representative strains of Brucella. Phylogenetic relationships were inferred from sequences of housekeeping genes. Gene loss and acquisition of different Brucella species were inferred. A total of 214 genes were found to be differentially distributed,and 173 of them were clustered into 15 genomic islands (GIs). Evidence of horizontal gene transfer was observed for 10GIs. Phylogenetic analysis indicated that the 19 strains formed five clades, and some of the GIs had been lost or acquired independently among the different lineages. The derivation of Brucella lineages is concomitant with the parallel loss or acquisition of GIs, indicating a complex interaction between various Brucella species and hosts.

Comparison of cecal microbiota composition in hybrid pigs from two separate three-way crosses

Yang, Yuting,Shen, Liyan,Gao, Huan,Ran, Jinming,Li, Xian,Jiang, Hengxin,Li, Xueyan,Cao, Zhenhui,Huang, Ying,Zhao, Sumei,Song, Chunlian,Pan, Hongbin Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.7

Objective: The intestinal microbiota plays an important role in host physiology, metabolism, immunity, and behavior. And host genetics could influence the gut microbiota of hybrid animals. The three-way cross model is commonly utilized in commercial pig production; however, the use of this model to analyse the gut microbial composition is rarely reported. Methods: Two three-way hybrid pigs were selected, with Saba pigs as the starting maternal pig: Duroc× (Berkshire×Saba) (DBS) pig, Berkshire×(Duroc×Saba) (BDS) pig. One hundred pigs of each model were reared from 35 days (d) to 210 d. The body weight or feed consumption of all pigs were recorded and their feed/gain (F/G) ratio was calculated. On day 210, 10 pigs from each three-way cross were selected for slaughter, and cecal chyme samples were collected for 16S rRNA gene sequencing. Results: The final body weight (FBW) and average daily gain (ADG) of DBS pigs were significantly higher than those of BDS pigs (p<0.05), while the F/G ratios of DBS pigs were significantly lower than those of BDS pigs (p<0.05). The dominant phyla in DBS and BDS pigs were Bacteroidetes (55.23% vs 59%, respectively) and Firmicutes (36.65% vs 34.86%, respectively) (p>0.05). At the genus level, the abundance of Prevotella, Roseburia, and Anaerovibrio in DBS pigs was significantly lower than in BDS pigs (p<0.01). The abundance of Eubacterium, Clostridium XI, Bacteroides, Methanomassiliicoccus, and Parabacteroides in DBS pigs was significantly higher than in BDS pigs (p<0.05). The FBWs and ADGs were positively correlated with Bacteroides, ClostridiumXI, and Parabacteroides but negatively correlated with the Prevotella, Prevotella/Bacteroides (P/B) ratio, Roseburia, and Anaerovibrio. Conclusion: These results indicated that host genetics affect the cecal microbiota composition and the porcine gut microbiota is associated with growth performance, thereby suggesting that gut microbiota composition may be a useful biomarker in porcine genetics and breeding.