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Study on Spinnability of PP/PU Blends and Preparation of PP/PU Bi-component Melt Blown Nonwovens
Yanfen Zhou,Liang Jiang,Huiying Jia,Xiaolu Xing,Zhenhua Sun,Shaojuan Chen,Jianwei Ma,Stephen Jerrams 한국섬유공학회 2019 Fibers and polymers Vol.20 No.6
Melt blown polymer blends offers a good way to combine two polymers in the same fiber generating nonwovenswith new and novel properties. In this study, polypropylene (PP) and polyurethane (PU) were blended to prepare PP/PU bicomponentmelt blown nonwovens. The spinnability of PP/PU composites was investigated and PP/PU bi-componentnonwovens with compositions of 95/5, 90/10, 80/20 and 70/30 were prepared by using the melt blowing technique. The meltblown fibers exhibited a ‘sea-island’ structure with PP as the continuous phase and PU as the dispersed phase. When thecontent of PU in the blend was above 40 %, PP/PU melt blown nonwovens could not be produced due to fiber breaking. ForPP/PU (90/10) nonwovens, it was found that the average fiber diameter decreased with increasing die to collector (DCD) andelevated hot air pressure.
Shuyi Yuan,Yanfen Liu,Yun Mu,Yongshen Kuang,Shaohong Chen,Yun-Tao Zhao,You Liu The Korean Society of Veterinary Science 2024 Journal of Veterinary Science Vol.25 No.1
Background: Peste des petits ruminants (PPR) is a contagious and fatal disease of sheep and goats. PPR virus (PPRV) infection induces endoplasmic reticulum (ER) stress-mediated unfolded protein response (UPR). The activation of UPR signaling pathways and their impact on apoptosis and virus replication remains controversial. Objectives: To investigate the role of PPRV-induced ER stress and the IRE1-XBP1 and IRE1-JNK pathways and their impact on apoptosis and virus replication. Methods: The cell viability and virus replication were assessed by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay, immunofluorescence assay, and Western blot. The expression of ER stress biomarker GRP78, IRE1, and its downstream molecules, PPRV-N protein, and apoptosis-related proteins was detected by Western blot and quantitative reverse transcription-polymerase chain reaction, respectively. 4-Phenylbutyric acid (4-PBA) and STF-083010 were respectively used to inhibit ER stress and IRE1 signaling pathway. Results: The expression of GRP78, IRE1α, p-IRE1α, XBP1s, JNK, p-JNK, caspase-3, caspase-9, Bax and PPRV-N were significantly up-regulated in PPRV-infected cells, the expression of Bcl-2 was significantly down-regulated. Due to 4-PBA treatment, the expression of GRP78, p-IRE1α, XBP1s, p-JNK, caspase-3, caspase-9, Bax, and PPRV-N were significantly downregulated, the expression of Bcl-2 was significantly up-regulated. Moreover, in PPRV-infected cells, the expression of p-IRE1α, p-JNK, Bax, and PPRV-N was significantly decreased, and the expression of Bcl-2 was increased in the presence of STF-083010. Conclusions: PPRV infection induces ER stress and IRE1 activation, resulting in apoptosis and enhancement of virus replication through IRE1-XBP1s and IRE1-JNK pathways.
Zhiqing Jiang,Liang Jiang,Huiying Jia,Yanfen Zhou,Jianwei Ma,Shaojuan Chen 한국섬유공학회 2018 Fibers and polymers Vol.19 No.8
The surface of polytetrafluoroethylene (PTFE)-fiberglass composite film was modified with polydopamine (PDA) in order to improve hydrophilic properties and hence to expand its perspective usage for biomedical and blood-contacting applications. Scanning electron microscopy, atomic force microscopy, energy dispersive spectrometer and fourier transform infrared spectra were employed to analyse the surface morphology and the chemical structures of the modified PTFEfiberglass composite films. Hydrophilic property of PTFE-fiberglass composite films was investigated by using water contact angle measurement. The effect of treatment time on the surface morphology and hydrophilicity of PTFE-fiberglass composite films was investigated. The results showed that a dense layer of PDA was formed on PTFE-fiberglass composite films, the water contact angle decreased gradually with the increase in modification time. Moreover, the fastness of PDA layers deposited on the PTFE-fiberglass composite films was studied by using UV/VIS/NIR spectrometer. It was revealed that the PDA layer was stable in distilled water, 0.1 M hydrochloric acid solution and alcohol, but had a poor resistance to 0.1 M sodium hydroxide solution.
Parallel Gene Loss and Acquisition Among Strains of Different Brucella Species and Biovars
Zhijun Zhong,Yufei Wang,Jie Xu,Yanfen Chen,Yuehua Ke,Xiaoyan Zhou,Xitong Yuan,Dongsheng Zhou,Yi Yang,Ruifu Yang,Guangneng Peng,Hai Jiang,Jing Yuan,Hongbin Song,Buyun Cui,Liuyu Huang,Zeliang Chen 한국미생물학회 2012 The journal of microbiology Vol.50 No.4
The genus Brucella is divided into six species; of these, B. melitensis and B. abortus are pathogenic to humans, and B. ovis and B. neotomae are nonpathogenic to humans. The definition of gene loss and acquisition is essential for understanding Brucella’s ecology, evolutionary history, and host relationships. A DNA microarray containing unique genes of B. melitensis Type strain 16MT and B. abortus 9-941 was constructed and used to determine the gene contents of the representative strains of Brucella. Phylogenetic relationships were inferred from sequences of housekeeping genes. Gene loss and acquisition of different Brucella species were inferred. A total of 214 genes were found to be differentially distributed,and 173 of them were clustered into 15 genomic islands (GIs). Evidence of horizontal gene transfer was observed for 10GIs. Phylogenetic analysis indicated that the 19 strains formed five clades, and some of the GIs had been lost or acquired independently among the different lineages. The derivation of Brucella lineages is concomitant with the parallel loss or acquisition of GIs, indicating a complex interaction between various Brucella species and hosts.