Endothelial Progenitor Cells’ Classification and Application in Neurological Diseases

Jing-jing Yuan,Jing Yang,Shi-lei Sun,Rui Zhang,Yu-ming Xu 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.4

The therapeutic effects of endothelial progenitor cells (EPCs) on ischemic stroke have been extensively studied in recent years. However, the differences in early EPCs and endothelial outgrowth cells (EOCs) are still unclear. Clarifications of their respective properties and specific functioning characteristics contribute to better applications of EPCs in ischemic diseases. In this review, we discuss cellular origin, isolation, culture, surface markers of early EPCs and EOCs and relevant applications in neurological diseases. We conclude that EOCs possess all characteristics of true endothelial progenitors and have potent advantages in EPC-based therapies for ischemic diseases. A number of preclinical and clinical applications of EPCs in neurological diseases are under study. More studies are needed to determine the specific characteristics of EPCs and the relevant mechanisms of EPCs for neurological diseases.

MiR-193b enhanced proliferation and migration and inhibits apoptosis through targeting RAB7A in osteosarcoma cell

Zhang Yuan-yuan,Xu Hai-yan,Dai Jing-jing 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.1

Background Accumulating study indicated that microRNA (miRNA) played critical role in the osteosarcoma (OS). The role and mechanisms of miR-193b in OS cell lines were still unknown. Objective We resolved the miR-193b expression in OS cell line and normal cell by RT-PCR assay. The effects of upregulated miR-193b on OS cell proliferation, migration, invasion and apoptosis were evaluated using CCK-8 assay, transwell assay, would-healing assay and flow cytometric analysis in vitro, respectively. We investigated the effect of upregulated miR-193b on the mRNA level of cell cycle protein CCND1 and CCNE1 using RT-PCR assay and the protein level of epithelial to mesenchymal transition (EMT)-related protein E-cadherin, vimentin, and N-cadherin by western blotting assay in MG-63 and U2SO cells. Furthermore, luciferase reporter assays were employed to identify the candidate target gene RAB7A of miR-193b. Results The expression of miR-193b was downregulated in OS cells. In MG-63 and U2SO cells, ectopic miR-193b expression inhibited cell proliferation, migration, invasion, and induced apoptosis. We found that miR-193b reduced the mRNA expression of CCND1 and CCNE1, and regulated the associated proteins of EMT including E-cadherin, vimentin and N-cadherin in MG-63and U2SO cell lines. Moreover, the candidate target gene RAB7A was negatively regulated by miR-193b. In addition, upregulated RAB7A rescued the inhibitory effect of miR-193b mimics on the development of OS cell. Conclusion In conclusion, this study suggested that miR-193b overexpression inhibited cell proliferation, migration, invasion, and induced cell apoptosis by down-regulating RAB7A in OS cell lines.

The Study of China’s Military Strategy and Satellite Development: Moving Toward “Winning the Informationized War Globally”?

Yuan-Chou Jing 한국국방연구원 2019 The Korean Journal of Defense Analysis Vol.31 No.1

China’s military strategy has evolved across different leaders’ eras. Even though China’s military strategic guideline always stays on the track of “active defense,” the essence has been shifting from tradition to informationization. Since satellites are able to provide space information support so as to fulfill informationization, once informationization becomes the focus of military strategy, it is supposed to bring momentum to the research and development of satellites. Therefore, satellite development can be considered as an index to check whether the military strategy is being implemented or not. China is continuously making progress in satellite development. Especially since the informationization was included in the guideline in the Hu Jintao era, satellites started to be able to conduct space information support missions. In conclusion, China is sincerely fulfilling its military strategy through consistent satellite development. On the other hand, “local war” is another focus of strategic guidelines, it implies that China considers its national interests mainly concentrated on this region so far. However, while China’s interests are expanding globally, China will need to cultivate its global dominance based on rapid satellite development to safeguard the global interest; “Winning the informationized war globally” might be the next focus.

A Comparison of the Fitness Functions to Identify the Motor-Table System: Simulations and Experiments

Yuan-Chou Jing,Kun-Yung Chen 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.4

The different state-error fitness functions (FFs) are proposed and compared numerically and experimentally to identify a motor-table system by using self-learning particle swarm optimization (SLPSO). Firstly, the completed mathematical model containing both of mechanical and electrical equations is successfully formulated. Secondly, the FFs containing different state-errors are compared by using PSO and SLPSO to identify the unknown parameters. It is found that the identify performance of the SLPSO algorithm by using FF with full-state error of displacement, velocity and current is the best than the other methods. Thus, the FF with full-state errors is adopted in experiments for a real mechatronic motor-table system. Then, the unknown parameters are successfully identified by the SLPSO algorithm. The contributions of this paper are: (1) the more states of the system are measured and used in the FF, the more parameters of system are accurately identified by the proposed identification approach, (2) the FF with full-state errors is performed in a real mechatronic motor-table system, and the unknown parameters are successfully identified by the SLPSO algorithm in experimental results.

Performance of a Large Volume Liquid Scintillation Detector for the Measurement of Fast Neutrons

Jing-Jun Zhu,J.I. Lee,곽정원,Jin Li,Ke-Jun Kang,M.J. Hwang,M.J. Lee,S.C. Kim,S.S. Myung,S.Y. Kim,Y.D. Kim,Yuan-Jing Li,J. Lee,김선기,방형찬,H.J. Kim,이현수,한인식,권영준 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.47 No.1

The KIMS collaboration is an experimental group searching for the Weakly Interacting Massive Particle (WIMP) which is one of the strongest candidates of dark matter. In a WIMP search experiment, the neutron is an important background because the nuclear recoil signal of a neutron is indistinguishable from that of a WIMP. We have constructed a 20-liter liquid scintillation detector to measure the neutron background. We present the performance of the neutron detector, as well as its structure and electronics system. We have achieved a good neutron/gamma separation anda good position measurement. A position dependent energy calibration has also been performed to correct the nonlinear response of the detector for different particle incident positions.

Threat Perception and Chinese Security Policy after the Cold War

Yuan, Jing-dong The Center for International Studies, Inha Univers 1998 Pacific Focus Vol.13 No.1

First-principles Study of the Electronic Structure of Nonmetal-doped Anatase TiO2

Yuan Wang,Jing Ma,Jian-Ping Zhou,Xiao-Ming Chen,Jing-Zhou Wang 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.3

In this paper, we present a detailed study of the structure, defect formation energy, and electronic and magnetic properties of nonmetal-doped TiO2 by using the first-principles projector augmented wave (PAW) potential within the generalized gradient approximation (GGA). The formation energy reduces with increasing electronegativity of the dopant. After doping with nonmetal elements, some band gaps of the doped-TiO2 become narrow, and others become wide, in which impurity states appear in the band gap. The relative positions of the impurity states are much different, mainly caused by the different electronegativities of the nonmetal elements F, O, B, C and N. When H is added to achieve a charge balance, the impurity states approach the valence band maximum, because the electronegativity difference among the nonmetal elements is decreased. Therefore, nonmetal and H codoping is an effective way to improve the visible-light catalytic activity of anatase TiO2. In addition, N-doping and C-doping can cause spin polarization of the TiO2 electronic structure and form 1.0 μB and 2.0 μB magnetic moment, respectively.

A Switched Quasi-Z-Source Inverter with Continuous Input Currents

Jing Yuan,Yongheng Yang,Frede Blaabjerg 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

This paper proposes a modified Switched quasi-ZSource Inverter (Sq-ZSI). The proposed Sq-ZSI achieves continuous input currents with a larger conversion ratio by replacing one diode of the Diode-assisted Switched Boost Inverter (DASBI) with a capacitor. Moreover, the voltage stress of the power switches in the proposed Sq-ZSI is lower and the voltage stress of the capacitors remains the same. The proposed topology is benchmarked with other Z-source inverters using active switches in terms of conversion ratio, voltage gain, and stresses on the power switches and capacitors. Simulation and experimental tests are provided to validate the analysis.

2,5-Hexanedione induces apoptosis via a mitochondriamediated pathway in PC12 cells

Yuan Qi,Shuang-yue Li,Feng-yuan Piao,Zhe-min Wang,Ruo-lin Chen,Shuang Liu,Jing-shun Shen 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.1

2,5-Hexanedione (HD) is the main active metabolite of n-hexane and mediates the neurotoxicity of the parent compound. Studies suggested that apoptosis involved in HD neurotoxicity. However, the mechanism of HD-induced neuronal apoptosis remains unknown. To explore its underlying mechanism, we treated PC12 cells with 5, 10 and 20 mM HD for 24 h, respectively. We found that HD induced apoptotic death in PC12 cells in a dose-dependent manner. Moreover, HD down-regulated Bcl-2 expression, up-regulated Bax expression and Bax/Bcl-2 ratio, promoted the disruption of mitochondrial transmembrane potential, induced the release of cytochrome c from mitochondria, and increased the activity of caspase-3 in PC12 cells, which were all the key regulators of intrinsic apoptotic pathway. These results indicate that HD induces apoptosis via a mitochondria- mediated pathway in PC12 cells.

Muscle fatigue: general understanding and treatment

Jing-jing Wan,Zhen Qin,Peng-yuan Wang,Yang Sun,Xia Liu 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Muscle fatigue is a common complaint in clinical practice. In humans, muscle fatigue can be defined as exercise-induced decrease in the ability to produce force. Here, to provide a general understanding and describe potential therapies for muscle fatigue, we summarize studies on muscle fatigue, including topics such as the sequence of events observed during force production, in vivo fatigue-site evaluation techniques, diagnostic markers and non-specific but effective treatments.