Evaluation of the Luminex ARIES HSV 1&2 Assay and Comparison with the FTD Neuro 9 and In-house Real-Time PCR Assays for Detecting Herpes Simplex Viruses

Chun Kiat Lee,Chean Nee Chai,Sharah Mae Capinpin,Alynn Ang,Sau Yoke Ng,Peak Ling Lee,Christopher Wai Siong Ng,Gabriel Yan,Hong Kai Lee,Lily-Lily Chiu,Roland Jureen,Benedict Yan,Tze Ping Loh 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.5

Background: Human herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) are responsible for a plethora of human diseases, of which cutaneous and mucocutaneous infections are the most prevalent. In its most severe form, HSV infection can cause meningitis/encephalitis. We compared the Luminex ARIES HSV 1&2 assay (Luminex Corp., Austin, TX, USA), an automated sample-to-result molecular solution, to two non-automated HSV DNA assays. Methods: A total of 116 artificial controls were used to determine the analytical performance of the ARIES assay. Controls were prepared by spiking universal transport medium (UTM) and cerebrospinal fluid (CSF) samples from patients who tested negative for HSV by an in-house HSV-1 and -2 DNA assay with reference materials (SeraCare Life Sciences, MA, USA; ZeptoMetrix Corp., MA, USA). Another 117 clinical samples were then used to compare the clinical performance of the ARIES assay with those of an in-house assay and the FTD Neuro 9 assay (Fast Track Diagnostics, Junglinster, Luxembourg). Results: The analytical sensitivity (95% limit of detection) of the ARIES assay was 318 copies/mL (UTM samples) and 935 copies/mL (CSF samples) for HSV-1 strain 96 and 253 copies/mL (UTM samples) and 821 copies/mL (CSF samples) for HSV-2 strain 09. No cross-reactivity was observed in samples spiked with 14 non-HSV microorganisms. Compared with the reference result (agreement between the in-house and FTD Neuro 9 results), the ARIES assay had overall concordance rates of 98.2% (111/113) and 100% (113/113) for HSV-1 and HSV-2, respectively. Conclusions: The ARIES assay appears to be an excellent alternative for rapid detection and differentiation of HSV in skin and genital infections, meningitis, and encephalitis.

Photochromic Behavior and Its Stability of a New Bifunctional Dye Composed of Spirobenzopyran and a Cinnamoyl Moiety

Kai Hua Shen,Jae Hong Kim,Go Woon Kim,Min Ju Cho,Sang Kyu Lee,Dong Hoon Choi 한국고분자학회 2005 Macromolecular Research Vol.13 No.3

Nonruminant Nutrition : Evaluation of Un-fasted Pig Stomach Spent Feed as a Substitute in Finishing Pigs Diet

( Kai Kaingmean ),( Jun Yeob Lee ),( Myeong Ho Lee ),( Sang Yun Ji ),( Hong Gil Moon ),( Sang Jip Ohh ) 한국동물자원과학회(구 한국축산학회) 2011 한국축산학회지 Vol.53 No.6

Insufficient pre-slaughter fasting leaves serious amount of feed-like contents(designated here as un-fasted stomach spent feed, USSF) in the eviscerated pig stomach. This study was intended to evoke economical and environmental seriousness of USSF discharge by estimating its value as pig feed. For finishing pigs feeding trial, three levels(0, 5, and 10%) of USSF were blended with pig feed to prepare control and two treatment diets, respectively. A total of 42(21 males, 21 females) crossbred (Landrace× Yorkshire×Duroc) finishing pigs weighing 81.5±8.0kg were employed to 28d feeding trial and in vivo digestibility trial by Cr2O3 indicator method with 7 males and 7 female pigs per treatment. In vitro total tract digestion of USSF showed 70.5% and 57.6% of DM and OM digestibilities, respectively which were poorer(p<0.05) than those of pig diet. There were no differences in body weight gain, daily feed intake and feed conversion ratio among treatments although 10% USSF substitution exerted relatively poor performance. In vivo apparent digestibilities of diets containing USSF 5% and 10% were lower(p<0.05) than that of 100% pig feed. There were no differences(p>0.05) in dressing percentage and carcass grade among treatments. Results of this study showed that 5% USSF substitution in finishing pigs diet did not exert any disadvantage in terms of production performance and carcass grade. This study implied that un-fasted slaughter causing excessive excretion of USSF should be avoided. If not avoidable, the USSF should not be wasted in abattoir but could be recycled as pig feed.

Design of Birefringence Lens Arrays for Continuous Enhanced Depth of Field

Kai Xu(서개),Hong-Shik Lee(이홍식),Yong-Seok Hwang(황용석),Sang-Shin Lee(이상신) 한국통신학회 2009 한국통신학회 워크샵 Vol.2009 No.6

마르코스 노박(Marcos Novak)의 트렌스 건축(Trans Architecture) 개념에 관한 연구

이규홍(Lee, Kyu-Hong),김개천(Kim, Kai-Chun) 한국실내디자인학회 2006 한국실내디자인학회논문집 Vol.15 No.4

Comparison of Luminex NxTAG Respiratory Pathogen Panel and xTAG Respiratory Viral Panel FAST Version 2 for the Detection of Respiratory Viruses

Chun Kiat Lee,Hong Kai Lee,Christopher Wei Siong Ng,Lily Chiu,Julian Wei-Tze Tang,Tze Ping Loh,Evelyn Siew-Chuan Koay 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.3

Owing to advancements in molecular diagnostics, recent years have seen an increasing number of laboratories adopting respiratory viral panels to detect respiratory pathogens. In December 2015, the NxTAG respiratory pathogen panel (NxTAG RPP) was approved by the United States Food and Drug Administration. We compared the clinical performance of this new assay with that of the xTAG respiratory viral panel (xTAG RVP) FAST v2 using 142 clinical samples and 12 external quality assessment samples. Discordant results were resolved by using a laboratory-developed respiratory viral panel. The NxTAG RPP achieved 100% concordant negative results and 86.6% concordant positive results. It detected one coronavirus 229E and eight influenza A/H3N2 viruses that were missed by the xTAG RVP FAST v2. On the other hand, the NxTAG RPP missed one enterovirus/rhinovirus and one metapneumovirus that were detected by FAST v2. Both panels correctly identified all the pathogens in the 12 external quality assessment samples. Overall, the NxTAG RPP demonstrated good diagnostic performance. Of note, it was better able to subtype the influenza A/H3N2 viruses compared with the xTAG RVP FAST v2.

Comparison of Serum Ketone Levels and Cardiometabolic Efficacy of Dapagliflozin versus Sitagliptin among Insulin-Treated Chinese Patients with Type 2 Diabetes Mellitus

Chi-Ho Lee,Mei-Zhen Wu,David Tak-Wai Lui,Darren Shing-Hei Chan,Carol Ho-Yi Fong,Sammy Wing-Ming Shiu,Ying Wong,Alan Chun-Hong Lee,Joanne King-Yan Lam,Yu-Cho Woo,Karen Siu-Ling Lam,Kelvin Kai-Hang Yiu 대한당뇨병학회 2022 Diabetes and Metabolism Journal Vol.46 No.6

Background: Insulin-treated patients with long duration of type 2 diabetes mellitus (T2DM) are at increased risk of ketoacidosis related to sodium-glucose co-transporter 2 inhibitor (SGLT2i). The extent of circulating ketone elevation in these patients remains unknown. We conducted this study to compare the serum ketone response between dapagliflozin, an SGLT2i, and sitagliptin, a dipeptidyl peptidase-4 inhibitor, among insulin-treated T2DM patients.Methods: This was a randomized, open-label, active comparator-controlled study involving 60 insulin-treated T2DM patients. Participants were randomized 1:1 for 24-week of dapagliflozin 10 mg daily or sitagliptin 100 mg daily. Serum β-hydroxybutyrate (BHB) levels were measured at baseline, 12 and 24 weeks after intervention. Comprehensive cardiometabolic assessments were performed with measurements of high-density lipoprotein cholesterol (HDL-C) cholesterol efflux capacity (CEC), vibration-controlled transient elastography and echocardiography.Results: Among these 60 insulin-treated participants (mean age 58.8 years, diabetes duration 18.2 years, glycosylated hemoglobin 8.87%), as compared with sitagliptin, serum BHB levels increased significantly after 24 weeks of dapagliflozin (P=0.045), with a median of 27% increase from baseline. Change in serum BHB levels correlated significantly with change in free fatty acid levels. Despite similar glucose lowering, dapagliflozin led to significant improvements in body weight (P=0.006), waist circumference (P=0.028), HDL-C (P=0.041), CEC (P=0.045), controlled attenuation parameter (P=0.007), and liver stiffness (P=0.022). Average E/e’, an echocardiographic index of left ventricular diastolic dysfunction, was also significantly lower at 24 weeks in participants treated with dapagliflozin (P=0.037).Conclusion: Among insulin-treated T2DM patients with long diabetes duration, compared to sitagliptin, dapagliflozin modestly increased ketone levels and was associated with cardiometabolic benefits.

혼합영양생물인 Scenedesmus acuminatus의 반연속 배양 시 성장특성 연구

고시원(Suyan Gao),홍가이(Kai Hong),이태윤(Taeyoon Lee) 大韓環境工學會 2017 대한환경공학회지 Vol.39 No.6

본 연구는 혼합영양생물인 Scenedesmus acuminatus의 최적배양조건을 찾기 위한 연구로서 최적 공기주입량, 아세테이트 주입량, CO2 주입에 대해 실험을 진행하였다. 공기주입량은 0.72 vvm에서 최대 비성장속도와 최대바이오매스 생산량을 얻을 수 있었으며, 0.3 M의 아세테이트를 주입하였을 때 최대 비성장속도와 최대바이오매스 생산량을 얻었다. 반연속배양에서는 추가로 CO2 주입이 배양에 미치는 영향을 파악하였다. CO2 주입 실험에서는 아세테이트로 50% 치환한 후에 최대비성장속도(0.460 d<SUP>-1</SUP>)와 최대바이오매스 생산성(0.936 g L<SUP>-1</SUP> d<SUP>-1</SUP>)을 얻을 수 있었으며 이후 치환이 계속될수록 최대비성장속도와 최대바이오매스 생산성은 지속적으로 감소하였다. 공기주입 실험에서는 JM 배지로 배양할 때 가장 높은 비성장속도(0.381 d<SUP>-1</SUP>)와 최대바이오매스 생산성(0.253 g L<SUP>-1</SUP> d<SUP>-1</SUP>)을 보여주었지만 이후 아세테이트를 함유한 배지로 50% 치환하였을 때 오히려 초기값보다 감소하는 것을 관찰하였다. The purpose of this study was to determine optimum value of aeration, acetate dosage, and CO2 input for the cultivation of Scenedesmus acuminatus. Highest specific growth rate and maximum biomass productivity was obtained by the aeration of 0.72 vvm and lower specific growth rates and maximum biomass productivity were obtained for other aeration tests. When putting 0.3 M of ammonium acetate in JM medium, the highest specific growth rate and maximum biomass productivity were obtained. CO2 input tests were performed during semi-continuous culturing tests. The highest specific growth rate (0.460 d<SUP>-1</SUP>) and maximum biomass productivity (0.936 g L<SUP>-1</SUP> d<SUP>-1</SUP>) were obtained after replacing 50% of solution with 0.3 M of acetate solution for CO2 input tests. However, more dilutions after the first dilution resulted in lower specific growth rate and maximum biomass productivity. In aeration tests, the highest specific growth rate (0.381 d<SUP>-1</SUP>) and maximum biomass productivity (0.253 g L<SUP>-1</SUP>d<SUP>-1</SUP>) were obtained when cultivating it with JM medium, but the specific growth rate and maximum biomass producitivty were significantly decreased when 50% of solution was replaced by acetate containing solution.

Quantitative Peptidomics Study Reveals That a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato

Chen, Ying-Lan,Lee, Chi-Ying,Cheng, Kai-Tan,Chang, Wei-Hung,Huang, Rong-Nan,Nam, Hong Gil,Chen, Yet-Ran American Society of Plant Biologists 2014 The Plant cell Vol.26 No.10

<P>CAPE1, a conserved peptide elicitor derived from tomato PR-1, was induced by wounding and found to regulate immune responses against biological threats. As PR-1 is highly conserved across many organisms and the putative peptide from AtPR1 was also found to be bioactive in <I>Arabidopsis</I>, the results suggest that this peptide may be useful for enhancing resistance to stress in other plant species.</P><P>Many important cell-to-cell communication events in multicellular organisms are mediated by peptides, but only a few peptides have been identified in plants. In an attempt to address the difficulties in identifying plant signaling peptides, we developed a novel peptidomics approach and used this approach to discover defense signaling peptides in plants. In addition to the canonical peptide systemin, several novel peptides were confidently identified in tomato (<I>Solanum lycopersicum</I>) and quantified to be induced by both wounding and methyl jasmonate (MeJA). A wounding or wounding plus MeJA-induced peptide derived from the pathogenesis-related protein 1 (PR-1) family was found to induce significant antipathogen and minor antiherbivore responses in tomato. This study highlights a role for PR-1 in immune signaling and suggests the potential application of plant endogenous peptides in efforts to defeat biological threats in crop production. As PR-1 is highly conserved across many organisms and the putative peptide from At-PR1 was also found to be bioactive in <I>Arabidopsis thaliana</I>, our results suggest that this peptide may be useful for enhancing resistance to stress in other plant species.</P>

혼합영양 배양조건에서의 Anabena 배양을 위한 유기탄소(acetate 종류 및 농도) 선정 연구

홍카이,고시원,이태윤,Hong, Kai,Gao, Siyuan,Lee, Taeyoon 한국화학공학회 2018 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.56 No.1

본 연구는 혼합영양 배양 조건에서 acetate의 주입이 Anabena azollae의 성장에 미치는 영향을 파악하기 위해 수행되었다. 4가지 종류의 acetate 중 ethyle acetate가 Anabena azollae의 성장에 가장 효과적이라고 밝혀졌으며, 주입한 ethyle acetate의 농도가 증가할수록 성장속도는 증가하는 것을 확인하였다. 40 mM의 ethyl acetate의 경우 비성장속도는 $0.979day^{-1}$, 최대바이오매스 생산성은 $0.293g\;L^{-1}\;d^{-1}$로 본 연구에서 배양속도가 가장 빠른 것으로 판명되었다. Acetic acid와 butyl acetate의 경우 Anabena azollae 성장을 방해하는 것으로 나타났다. Aetration의 경우 0.54 vvm에서 성장속도가 가장 빨랐다. 반연속배양에서는 aeration 실험이 끝난 후 연속하여 ethyle acetate 주입을 하여 배양을 하였다. 회분식실험에 비해 반연속배양에서의 비성장속도와 최대바이오매스 생산성은 모두 감소하였지만 최대 농도는 5.91 g/L로 가장 큰 값을 나타내었다. The main objective of this study was to evaluate the effects of acetate on the cultivation of anabena under mixotrophic condition. Four different types of acetates were used for the anebena cultivation. Among them, ethyl acetate was found to be the most effective and the growth rates linearly increased as the amount of ethyl acetate increased. When 40 mM of ethyl acetate was used, the highest values of specific growth rate of $0.979day^{-1}$ and maximum biomass productivity of $0.293g\;L^{-1}\;d^{-1}$ were obtained. On the contrary, input of acetic acid and butyl acetate inhibited the growth of anabena. For aeration tests, 0.54 vvm was optimum for anabena cultivation. For a semi-continuous cultivation test, ethyl acetate was used after 0.54 vvm test was finished. Then, test continued under 0.54 vvm and 40 mM of ethyl acetate. Lower specific growth rate and maximum biomass productivity were obtained compared to those from batch cultivation tests. However, the greatest maximum concentration of 5.91 g/L was obtained during the semi-continuous cultivation test.