Investigation on the interactions of scutellarin and scutellarein with bovine serum albumin using spectroscopic and molecular docking techniques

Hao Tang,Nian-Guang Li,Zhi-Hao Shi,Yu-Ping Tang,Qian-Ping Shi,Ze-Xi Dong,Peng-Xuan Zhang,Jin-ao Duan 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10

The binding abilities of scutellarin (Scu) andscutellarein (Scue) with bovine serum albumin (BSA) wereinvestigated using equilibrium dialysis, high performanceliquid chromatography, fluorescence spectroscopy, competitivesite marker and molecular docking. The resultsshowed that the average protein binding ratios of Scu andScue with BSA were (79.85 ± 1.83) and (85.49 ± 1.21) %respectively. Under simulated physiological conditions, thefluorescence data indicated that Scu and Scue bound withBSA through a static mechanism. The thermodynamicparameters indicated that the interactions of Scu-BSA andScue-BSA mainly occurred by van der Waals forces andhydrogen bonds and it was easier for Scue to bind withBSA than Scu, indicating that the glucuronic acid moleculein Scu decreased the binding affinity. Site competitivemarker experiments showed that the binding sites of Scuand Scue mainly located within the sub-domain IIA ofBSA. Furthermore, molecular docking studies indicatedthat one BSA could bind three Scue, while one BSA couldcarry only two Scu. All these results clearly indicated theinteractions of Scu and Scue with BSA, which will lay thefoundation for further research to determine the pharmacologyand pharmacodynamics of Scu and Scue for treatingischemic cerebrovascular disease.

Mobile Robot Navigation based on Human Walking Trajectory in Intelligent Space

Peng Duan,Guohui Tian,Hao Wu 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.11

This paper presents a mobile robot navigation method based on human walking trajectory in intelligent space. Unlike the traditional navigation methods, the proposed method enables the robot to walk a meaningful path, which reflects human activity patterns in daily life. Human walking trajectories are extracted using multiple distributed vision cameras in intelligent space. To group trajectories with the same direction, trajectory similarity measurement is implemented. Key points on human walking trajectories and typical transfer points are utilized to build a topological map. To navigate a mobile robot to walk along the trajectory of human walking, global path planning based on the topological map and local path planning based on gird map are carried out with the spared information in intelligent space. Experimental results illustrate the performance of the proposed navigation method.

THE “BACKFIRE” EFFECTS OF LUXURY ADVERTISING ON SHORT-FORM VIDEO SOCIAL MEDIA

Hao Zhang,Gongming Duan 글로벌지식마케팅경영학회 2021 Global Fashion Management Conference Vol.2021 No.11

Impact of nanocomposite material to counter injury in physical sport in the tennis racket

Hao Jin,Bo Zhang,Xiaojing Duan Techno-Press 2023 Advances in nano research Vol.14 No.5

Sports activities, including playing tennis, are popular with many people. As this industry has become more professionalized, investors and those involved in sports are sure to pay attention to any tool that improves athletes' performance Tennis requires perfect coordination between hands, eyes, and the whole body. Consequently, to perform long-term sports, athletes must have enough muscle strength, flexibility, and endurance. Tennis rackets with new frames were manufactured because tennis players' performance depends on their rackets. These rackets are distinguished by their lighter weight. Composite rackets are available in many types, most of which are made from the latest composite materials. During physical exercise with a tennis racket, nanocomposite materials have a significant effect on reducing injuries. Materials as strong as graphite and thermoplastic can be used to produce these composites that include both fiber and filament. Polyamide is a thermoplastic typically used in composites as a matrix. In today's manufacturing process, materials are made more flexible, structurally more vital, and lighter. This paper discusses the production, testing, and structural analysis of a new polyamide/Multi-walled carbon nanotube nanocomposite. This polyamide can be a suitable substitute for other composite materials in the tennis racket frame. By compression polymerization, polyamide was synthesized. The functionalization of Multi-walled carbon nanotube (MWCNT) was achieved using sulfuric acid and nitric acid, followed by ultrasonic preparation of nanocomposite materials with weight percentages of 5, 10, and 15. Fourier transform infrared (FTIR) and Nuclear magnetic resonance (NMR) confirmed a synthesized nanocomposite structure. Nanocomposites were tested for thermal resistance using the simultaneous thermal analysis (DTA-TG) method. scanning electron microscopy (SEM) analysis was used to determine pores' size, structure, and surface area. An X-ray diffraction analysis (XRD) analysis was used to determine their amorphous nature.

CHARACTERISTICS OF GEOPHYSICAL FIELD IN THE EAST CHINA AND ADJACENT REGIONS AND ITS GEOTECTONIC EVOLUTION

HAO TIANYAO,LIU YIKE,DUAN CHANG 대한지질학회 1997 대한지질학회 학술대회 Vol.1997 No.

Concurrent Hypermethylation of SFRP2 and DKK2 Activates the Wnt/β-Catenin Pathway and Is Associated with Poor Prognosis in Patients with Gastric Cancer

Hao Wang,Xiang-Long Duan,Xiao-Li Qi,Lei Meng,Yi-Song Xu,Tong Wu,Peng-Gao Dai 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.1

Aberrant hypermethylation of Wnt antagonists has been observed in gastric cancer. A number of studies have focused on the hypermethylation of a single Wnt antagonist and its role in regulating the activation of signaling. However, how the Wnt antagonists interacted to regulate the signaling pathway has not been reported. In the present study, we systematically investigated the methylation of some Wnt antagonist genes (SFRP2, SFRP4, SFRP5, DKK1, DKK2, and APC) and their regulatory role in carcinogenesis. We found that aberrant promoter methylation of SFRP2, SFRP4, DKK1, and DKK2 was significantly increased in gastric cancer. Moreover, concurrent hypermethylation of SFRP2 and DKK2 was observed in gastric cancer and this was significantly associated with increased expression of -catenin, indicating that the joint inactivation of these two genes promoted the activation of the Wnt signaling pathway. Further analysis using a multivariate Cox proportional hazards model showed that DKK2 methylation was an independent prognostic factor for poor overall survival, and the predictive value was markedly enhanced when the combined methylation status of SFRP2 and DKK2 was con-sidered. In addition, the methylation level of SFRP4 and DKK2 was correlated with the patient’s age and tumor differentiation, respectively. In conclusion, epigenetic silencing of Wnt antagonists was associated with gastric carcinogenesis, and concurrent hypermethylation of SFRP2 and DKK2 could be a potential marker for a prognosis of poor overall survival.

Apply Partition Tree to Compute Canonical Labelings of Graphs

HAO Jian-Qiang,GONG Yun-Zhan,Tan Li,Duan Da-Gao 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.5

This paper establishes a theoretical framework by defining a set of concepts useful for classifying graphs and computing the canonical labeling Cmax(G) of a given undirected graph G, which including the partition tree PartT(G), maximum partition tree MaxPT(G), centre subgraph Cen(G), standard regular sequence SRQ(G), standard maximum regular sequence SMRQ(G), and so on. The implementations of algorithms 1 to 5 show how to calculate them accordingly. The worst time complexities of algorithms 1, 2, 4, and 5 are O(n2) respectively. The time complexity of Algorithm 3 is O(n). By Theorem 3, all leaf nodes of PartT(G) and MaxPT(G) are the regular subgraphs. By Theorem 4 and 5, there exists only one Cen(G) in G. Regular Partition Theorem 6 shows that there exists just one corresponding PartT(G), SRQ(G), MaxPT(G), and SMRQ(G). One can use Classification Theorem 7 to category graphs. Theorem 8 and 9 establish the link between the Cen(G) and the calculation of the first node u1 added into MaxQ(G) corresponding to the canonical labeling Cmax(G) of G. Further, it utilizes the Cen(G) to calculate the first node u1 added into MaxQ(G). The proposed methods can be extended to deal with the directed graphs and weighted graphs.

B3GNT2, a Polylactosamine Synthase, Regulates Glycosylation of EGFR in H7721 Human Hepatocellular Carcinoma Cells

Qiu, Hao,Duan, Wei-Ming,Shu, Jie,Cheng, Hong-Xia,Wang, Wei-Ping,Huang, Xin-En,Chen, Hui-Li Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

The epidermal growth factor receptor (EGFR) is an important surface receptor with N-glycans in its extracellular domain, whose glycosylation is essential for its function, especially in tumor cells. Here, we demonstrated that polylactosamine is markedly increased in H7721 hepatocellular carcinoma cells after treatment with EGF, while it apparently declined after exposure to all-trans retinoic acid (ATRA). In the study of the enzymatic mechanism of this phenomenon, we explored changes in the expression of poly-N-acetyllactosamine (PLN) branching glycosyltransferases using RT-PCR. Among the four glycosyltransferases with altered expression, GnT-V was most elevated by EGF, while GnT-V and B3GNT2 were most declined by ATRA. Next, we conducted co-immunoprecipitation experiments to test whether B3GNT2 and EGFR associate with each other. We observed that EGFR is a B3GNT2-targeting protein in H7721 cells. Taken together, these findings indicated that the altered expression of B3GNT2 will remodel the PLN stucture of EGFR in H7721 cells, which may modify downstream signal transduction.

NUMERICAL SIMULATION AND PREDICTION OF NEGATIVE STORM SURGE IN THE YANGTZE RIVER ESTUARY

Zeng Hao Qin,Yi Hong Duan,De Bao Hu,Mao Xing Gong 한국해안해양공학회 1999 학술강연회 발표논문초록집 Vol.1 No.1

An attempt is made by using both statistical model and multi-layer simplified ECOM-Si model with temperature and salinity effects neglected to investigate numerically the negative storm surge (SS) in the Yangtze River estuary. An operational system for predicting the negative SS are constructed in conjunction of ECOM-Si model with the operational limited-area fine-mesh numerical weather prediction (NWP) model developed by the Shanghai Typhoon Institute(STI).To test the reliability of ECOM-Si model and to understand the computational feature of the model in simulating sea surface elevation, both semi-diurnal tide computation at the river mouth tidal station and numerical SS simulations under the action of idealized easterly and westerly winds are completed. Based on these experiments, a series of negative SS simulations induced by the cold wave, tropical and extratropical cyclones are carried out respectively, in which the objective analysis data of atmospheric forcings on the sea surface are offered by STI NWP model. Case experiments show that the predicted peak negative SS are coincident with the real ones and the phase differences between them are within a acceptable limit. The system is hopeful in operational use for predicting negative SS in the Yangtze River estuary.

Virtual Environment Modeling for Battery Management System

Chang-hao Piao,Qi-fan Yu,Chong-xi Duan,Ling Su,Yan Zhang 대한전기학회 2014 Journal of Electrical Engineering & Technology Vol.9 No.5

The offline verification of state of charge estimation, power estimation, fault diagnosis and emergency control of battery management system (BMS) is one of the key technologies in the field of electric vehicle battery system. It is difficult to test and verify the battery management system software in the early stage, especially for algorithms such as system state estimation, emergency control and so on. This article carried out the virtual environment modeling for verification of battery management system. According to the input/output parameters of battery management system, virtual environment is determined to run the battery management system. With the integration of the developed BMS model and the external model, the virtual environment model has been established for battery management system in the vehicle’s working environment. Through the virtual environment model, the effectiveness of software algorithm of BMS was verified, such as battery state parameters estimation, power estimation, fault diagnosis, charge and discharge management, etc.