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      • 환경기초시설의 인 기준 강화에 따른 팔당호 유입 수계의 수질개선 효과분석

        정원구,한영한,임재명 江原大學校 産業技術硏究所 2010 産業技術硏究 Vol.30 No.B

        The influences on water quality of each river by effluents from environmental facilities located in 14 unit watersheds of North- and South-Han River, and Gyungan-cheon were analyzed. Also, the water quality modeling for study area was carried out to analyze the improvement effect of water quality by the strengthening of T-P effluent standard of environmental facilities. For the calibration and verification of model, water quality data and effluent loading calculated for 2006 were used. Data of low water period were used for calibration, and normal water period for verification. The results of calibration and verification were well matched with the real water quality dataset of revers. Also, the validity of the results were estimated using RI(Reliability Index) method. When the T-P effluent standards for environmental facilities were strengthened, T-P concentrations were predicted to improve from 0.025㎎/ℓ to 0.023㎎/ℓ in the outlet location of North-Han River, from 0.056㎎/ℓ to 0.040㎎/ℓ for South-Han River, and from 0.233㎎/ℓ to 0.146㎎/ℓ for Gyungan-cheon. Also, the T-P concentrations of tributaries including Jojong-cheon, Dal-cheong, Sumgang, Chungmi-cheon, Bokha-cheon, Heuk-cheon, and Wonju-cheon were predicted to improve from 0.063㎎/ℓ to 0.010㎎/ℓ, from 0.091㎎/ℓ to 0.053㎎/ℓ, from 0.199㎎/ℓ to 0.100㎎/ℓ, from 0.168㎎/ℓ to 0.148㎎/ℓ, from 0.186㎎/ℓ to 0.105㎎/ℓ, from 0.019㎎/ℓ to 0.013㎎/ℓ, and from 0.822㎎/ℓ to 0.236㎎/ℓ, respectively.

      • SCISCIESCOPUS

        Infrared multiple photon dissociation spectroscopy and density functional theory (DFT) studies of protonated permethylated β-cyclodextrin–water non-covalent complexes

        Lee, Sung-Sik,Park, Soojin,Kim, Ju-Young,Kim, Hye-Rim,Lee, Sungyul,Oh, Han Bin The Royal Society of Chemistry 2014 Physical chemistry chemical physics Vol.16 No.18

        <P>We present infrared multiple photon dissociation (IRMPD) spectroscopy and quantum chemical calculation results for the protonated permethylated β-cyclodextrin (CD)–water non-covalent complex, the simplest β-CD non-covalent complex, in the gas-phase. The IRMPD spectrum in the region 2700–3750 cm<SUP>−1</SUP> consisted of three strong peaks at 3096, 3315, and 3490 cm<SUP>−1</SUP>. These spectral features in the experimental IRMPD spectrum were compared with a large set of infrared absorption spectra predicted using density functional theory (DFT) calculations for the protonated β-CD–water complex. Complex III (see Fig. 4c), in which the water molecule (at the primary rim) and the proton (at the secondary rim) were separated, was found to suitably reflect the main spectral characteristics found in the experimental IRMPD spectrum. The absence of the homodromic hydrogen bond ring, due to replacement of hydroxyl groups with methoxy groups in permethylated β-CD, rendered the primary rim open compared with the unmodified β-CD ‘one-gate-closed’ lowest energy conformer. This study demonstrates that IRMPD studies combined with DFT theoretical calculations can be a good method for studying molecular interactions of large host–guest pairs.</P> <P>Graphic Abstract</P><P>Water was found to interact with permethylated β-CD through multiple hydrogen bondings with methoxy groups of the rim. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cp54841d'> </P>

      • 호기성 고정생물막 반응조에서 인의 제거

        한동준,임재명,우영국 江原大學校 産業技術硏究所 1996 産業技術硏究 Vol.16 No.-

        While the enhanced biological phosphorus removal(EBPR), in anaerobic/aerobic condition, was known to remove phosphorus by means of metabolism of poly-Pmicroorganisms, the phosphorus removed could be released in the form of ortho-P in the aerobic fixed biofilm reactor. This study was initiated to investigate the cause of ortho-P release in the aerobi c fixed biofilm reactor. The resutls indicated that the phosporus release was caused by autooxidation. The synthesis and release of phosphrous were related to the ORP and the boundary value for the phase change was about 170mV. In the synthesis phase, the phosphorus removal rate per COD removed was 0.023mgP?/mgCOD?. The phosprous contents of the microorganism were 4.3~6.0% on a dry weight basis.

      • KCI등재

        John Dewey 敎育哲學의 批判 : 實用主義(Pragmatism)를 中心으로

        林漢永 연세대학교 인문과학연구소 1959 人文科學 Vol.4 No.-

        Since Dewey's death in 1952, many criticisms not only by American scholars but also those from Europe have been made. This article is based upon the attempts of H. H. Home, A. V. Judge, T. V. Rusk, Curtis, W. George Gergler and Russell, who have criticized Dewey's progressive philosophy of education. The criticism of main issues: 1.The main areas of criticism are denial of absolute values. 2.Lack of appreciation of factors beyond the scope of human experience. The author tries to resolve the differences between pragmatism and idealism and gets an idea to reconstruct "Neo-Deweyism for Korean-Education," which will be the main theme to be presented by the author later.

      • 내부탄소원을 이용한 돈사폐수의 탈질화

        한동준,임재명,우영국 江原大學校 産業技術硏究所 1996 産業技術硏究 Vol.16 No.-

        This reasearch aims to investigate the effects of an internal carbon source in the dentification of piggery wastewater. In this study, the raw wastewater and the effluent from each of anoxic basin and anaerobic basin were used as the internal carbon sources. The experiments were carried out in batch system and the results are as follows; i)Denitrification rastes were the highest in the raw wastewater asnd the lowest in the anaerobic effluent. ii)The piggery wastewater contained about 60 percent of the readily biodegradable organic(RDCOD), which led to a conclusion that the raw wastewater could be used as the internal carbon source for denitrification. For the efficient denitrificatuon, pre-denitrification process was found profitable. iii) In denitrification, alkalinity production rates were in the range of 3.4 to 3.6 mgCaCO?/mg NO?-N. iv) The denitritation of piggery wastewaster came out to be possible, and the rate of organic carbon consumption decreased about 10 percent.

      • SCOPUSKCI등재
      • SCIESCOPUSKCI등재
      • 肺吸蟲症에 있어서 尿를 이용한 免疫學的 診斷에 관한 硏究

        李榮喆,李駿商,林漢鍾 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.3

        Human paragonimiasis is one of the most common trematode diseases and of very important public health problems in Korea. Since neither skin test always represents current infection nor recovery of Puragovzinius westermani ova, the immunodiagnosis of human paragonimiasis is not always possible, a more feasible, sensitive and specific method is required. To investigate the feasibility and diagnostic accuracy of using the enzyme-linked immunosorbent assay (ELISA) with a urine sample in the immunodiagnosis of human paragonimiasis. 72 normal control subjects. 100 subjects with skin test positive for paragonimiasis. 19 patients with paragonimiasis. 13 subjects with serum optical density of more than 1.00 for Paragonimus antigen and clonorchiasis subjects were studied. ELISA was performed with Verona! buffered saline antigen of Paragonimus westermani. peroxidase conjugated anti-rabbit IgG and orthophenylene diamine as a substrate by micro-method. The results obtained are as follows: 1. The mean O.D. (optical density) values of ELISA of normal control subjects was 0.19±0.07 in the urine samples and 0.16 & 0.09 in the serum samples. 2. The mean O.D. values in the urine samples of 100 positives by skin test was 0.47±1 0.64 and in the serum samples was 0.65±0.72. The relation of the absorbance values between the urine (x-axis) and serum (y-axis) samples made the regression equation: y = 0.95x+0.206, and showed high correlation (r = 0.847, n = 100, p < 0.01). 3. Pseudopodia showed at the wheal by the skin test in the 12 (63%) out of 19 paragonimiasis. The mean O.D. values of 19 paragonimiasis was 1.41±0.58 in the urine samples and 1.64±0.38 in the serum samples. The O.D. values of confirmed paragocimiasis cases were 0.5 or over in the almost every cases using a urine sample. 4. Six cases of paragonimiasis which were treated effectively showed same reactions of the skin test and the O.D. values in the serum samples of other paragoniniiasis before treatment, but the O.D. values in the urine samples was a tendency of decrease. In conclusion, these findings suggested that ELISA using a urine sample was more feasible and sensitive in the immunodiagnosis of human paragonimiasis in the mass screening.

      • Immunoblot법을 이용한 Anisakis증의 혈청학적 진단

        이영주,주경환,정명숙,임한종 고려대학교 의과대학 1990 고려대 의대 잡지 Vol.27 No.1

        Acute gastric anisakiasis is rather easily diagnosed by endoscopy or radiological technique. But preoperational diagnosis of acute intestinal anisakiasis is very difficult. Furthermore, the diagnosis of chronic anisakiasis is more difficult because the worm invade the stomach or intestinal wall and finally to degenerate. Serodignosis may helpful in such diagnostic difficulties. However, any of the serodiagnostic method in anisakiasis has limitations for practical use due to cross reactions. In this context, the study was performed to observe the specifically reacting antigenic bands of crude extract Anisakis larval worm antigen and their reaction to the serum antibody obtained from experimentally immunized and infected rabbit, confirmed human anisakiasis and other helminthic infection cases using SDS-PAGE and EITB. The results obtained were as follows; 1. Among 12 cases of confirmed anisakiasis, eleven showed positive reactions by ELISA. The average 0. D. value of the test was 1.035. Two from 4 D. latum infected cases and one from 4 A. lumbricoides infected cases showed positive reaction respectively. 2. Silver stained SDS-PAGE of crude Anisakis larval worm antigen revealed more than 45 antigenic protein bands. Among them, 186, 76, 72, 65, 59~62, 54, 52, 50, 49, 46, 43, 40, 33, 32, 29, 24, 23, 18, 17, and 13Kd bands were major bands. Protein bands of 12Kd relative molecular weight was observed in A. suum extract antigen, on the other hand 29 and 46Kd bands were not observed. 3. Major antigenic bands of crude Anisakis worm antigen which reacted with serum antibody of confirmed anisakiasis cases were 186, 116, 72, 52, 46, 40, 33 and 32Kd band. Most of them also reacted with the sera of D. latum, A. lumbricoides, T. trichiura infected cases except 46Kd band which showed positive resuits only with sera of anisakiasis. So, this band could be considered as diagnostic band for anisakiasis especially for the identification of cross reactions against A. lumbricoides and D. lafum infection. 4. Although, low molecular weight bands such as 33 and 32Kd were observed as species specific, the sensitivity of that fractions were obviously low. Consequently, the diagnostic use of the low molecular weight protein bands were not promising. 5. Serum of rabbit immunized with Anisakis larval worm antigen also showed positive reaction on 46Kd bands, but that of A. suum and T. canis did not. These results were in accord with the results in human cases. In conclusion, 46Kd bands of crude Anisakis larval worm antigen can be considered as a diagnostic one for the identification of cross reactions using immunoblot technique.

      • 肝吸蟲症에 있어서 尿를 이용한 免疫學的 診斷에 관한 硏究

        金榮容,李駿商,林漢鍾 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.3

        Human clonorchiasis is one of the most common trematode disease and of very important public health problems in Korea. In this study, the enzyme-linked immunosorbent assay (ELISA) was employed in the diagnosis by use of urine samples in human clonorchiasis. ELISA was performed with veronal buffered saline antigen of Clonorchis, peroxidase conjugated antirabbit IgG and orthophenylenediamine as a substrate by micro-method. The results obtained are as follows; 1. The mean O.D. (optical density) values of ELISA in the urine samples of non-infected human cases was 0.037±0.087. If we proposed the O.D. value 0.1 as a differential point of positive reaction, the specificity ELISA in the urine samples was 93% (187 negative reaction out of 201 non-infected human cases). 2. The mear. O.D. valuses in the urine samples of clonorchiasis was 0.580±0.574 and in the serum samples of clonorchiasis was 1.930±0.623. The sensitivity of ELISA was 87.0% in the urine samples (409 positive reaction out of 470 clonorchiasis cases) and was 88.4% in the serum samples. 3. In the distribution of the O.D. values in the urine samples of clonorchhiasis, false positive rates of cross reaction between C. sinensis and P.westermant were 34.7% in the urine samples of clonorchiasis. 4. When the results were compared between the urine and the serum samples of 181 clonorchiasis showed that 88.7% gave same reactions. In the clonorchiasis cases, the O.D. values between the urine and the serum simples were significant at a level of p<0.01. 5. In rabbits, the O.D. values of the urine and the serum samples in 16weeks after infection with C. sinensis metacercariae to were greatly increased than those of the urine samples in preinfection. The O.D. values in the urine samples increased accoording to intensity and course of infection. In conclusion, results of this study suggest that ELISA using the urine samples is more applicable in the diagnosis of human clonorchiasis in mass screening.

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