Active control of amplitude and phase of high-power RF systems in EAST ICRF heating experiments

Zhu Guanghui,Liu Lunan,Mao Yuzhou,Zhang Xinjun,Guo Yaoyao,Ai Lin,Jiang Runhao,Qin Chengming,Zhang Wei,Yang Hua,Yuan Shuai,Wang Lei,Ju Songqing,Wang Yongsheng,Sun Xuan,Yang Zhida,Wang Jinxin,Cheng Yan 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.2

The EAST ICRF system operating space has been extended in power and phase control with a low-level RF system for the new double-strap antenna. Then the multi-step power and periodic phase scanning experiment were conducted in L-mode plasma, respectively. In the power scanning experiment, the stored energy, radiation power, plasma impedance and the antenna's temperature all have positive responses during the short ramp-ups of PL;ICRF. The core ion temperature increased from 1 keV to 1.5 keV and the core heating area expanded from jZj 5 cmto jZj 10 cm during the injection of ICRF waves. In the phasing scanning experiment, in addition to the same conclusions as the previous relatively phasing scanning experiment, the superposition effect of the fluctuation of stored energy, radiation power and neutron yield caused by phasing change with dual antenna, resulting in the amplitude and phase shift, was also observed. The active control of RF output facilitates the precise control of plasma profiles and greatly benefits future experimental exploration.

Linkage Disequilibrium Estimation of Chinese Beef Simmental Cattle Using High-density SNP Panels

Zhu, M.,Zhu, B.,Wang, Y.H.,Wu, Y.,Xu, L.,Guo, L.P.,Yuan, Z.R.,Zhang, L.P.,Gao, X.,Gao, H.J.,Xu, S.Z.,Li, J.Y. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.6

Linkage disequilibrium (LD) plays an important role in genomic selection and mapping quantitative trait loci (QTL). In this study, the pattern of LD and effective population size ($N_e$) were investigated in Chinese beef Simmental cattle. A total of 640 bulls were genotyped with IlluminaBovinSNP50BeadChip and IlluminaBovinHDBeadChip. We estimated LD for each autosomal chromosome at the distance between two random SNPs of <0 to 25 kb, 25 to 50 kb, 50 to 100 kb, 100 to 500 kb, 0.5 to 1 Mb, 1 to 5 Mb and 5 to 10 Mb. The mean values of $r^2$ were 0.30, 0.16 and 0.08, when the separation between SNPs ranged from 0 to 25 kb to 50 to 100 kb and then to 0.5 to 1 Mb, respectively. The LD estimates decreased as the distance increased in SNP pairs, and increased with the increase of minor allelic frequency (MAF) and with the decrease of sample sizes. Estimates of effective population size for Chinese beef Simmental cattle decreased in the past generations and $N_e$ was 73 at five generations ago.

Stress field interference of hydraulic fractures in layered formation

Zhu, Haiyan,Zhang, Xudong,Guo, Jianchun,Xu, Yaqin,Chen, Li,Yuan, Shuhang,Wang, Yonghui,Huang, Jingya Techno-Press 2015 Geomechanics & engineering Vol.9 No.5

Single treatment and staged treatments in vertical wells are widely applied in sandstone and mudstone thin interbedded (SMTI) reservoir to stimulate the reservoir. The keys and difficulties of stimulating this category of formations are to avoid hydraulic fracture propagating through the interface between shale and sand as well as control the fracture height. In this paper, the cohesive zone method was utilized to build the 3-dimensional fracture dynamic propagation model in shale and sand interbedded formation based on the cohesive damage element. Staged treatments and single treatment were simulated by single fracture propagation model and double fractures propagation model respectively. Study on the changes of fracture vicinity stress field during propagation is to compare and analyze the parameters which influence the interfacial induced stresses between two different fracturing methods. As a result, we can prejudge how difficult it is that the fracture propagates along its height direction. The induced stress increases as the pumping rate increasing and it changes as a parabolic function of the fluid viscosity. The optimized pump rate is $4.8m^3/min$ and fluid viscosity is $0.1Pa{\cdot}s$ to avoid the over extending of hydraulic fracture in height direction. The simulation outcomes were applied in the field to optimize the treatment parameters and the staged treatments was suggested to get a better production than single treatment.

Potential Mechanisms of Benzyl Isothiocyanate Suppression of Invasion and Angiogenesis by the U87MG Human Glioma Cell Line

Zhu, Yu,Zhang, Ling,Zhang, Guo-Dong,Wang, Hong-Ou,Liu, Ming-Yan,Jiang, Yuan,Qi, Li-Sha,Li, Qi,Yang, Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Glioma is one of the most common tumors in China and chemotherapy is critical for its treatment. Recent studies showed that benzyl isothiocyanate (BITC) could inhibit the growth of glioma cells, but the mechanisms are not fully understood. This study explored the inhibitory effect of BITC on invasion and angiogenesis of U87MG human glioma cells in vitro and in vivo, as well as potential mechanisms. It was found that BITC could inhibit invasion and angiogenesis of human glioma U87MG cells by inducing cell cycle arrest at phase G2/M. It also was demonstrated that BITC decreased expression of cyclin B1, p21, MMP-2/9, VE-cadherin, CD44, CXCR4 and MTH1, the activity of the telomerase and $PKC{\zeta}$ pathway. Microarray analysis was thus useful to explore the potential target genes related to tumorigenic processes. BITC may play important roles in the inhibition of invasion and angiogenesis of human glioma cells.

Human lactoferrin efficiently targeted into caprine beta-lactoglobulin locus with transcription activator-like effector nucleases

Yu-Guo Yuan,Shao-Zheng Song,Meng-Ming Zhu,Zheng-Yi He,Rui Lu,Ting Zhang,Fei Mi,Jin-Yu Wang,Yong Cheng 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8

Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT). Results: The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23%) were confirmed pregnant at 30 d. In second round SCNT, 7 (53%), 4 (31%), and 3 (23%) recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion: This finding signifies the combined use of TALENs and SCNT can generate bi-allelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.

Plasma Post-operative miR-21 Expression in the Prognosis of Gastric Cancers

Ma, Guo-Jian,Gu, Rong-Min,Zhu, Ming,Wen, Xu,Li, Jin-Tian,Zhang, Yuan-Ying,Zhang, Xiao-Mei,Chen, Sen-Qing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Tumor-associated microRNAs have been detected in serum or plasma, but whether plasma microRNA-21 (miR-21) could be a potential circulating biomarker for gastric cancer (GC) prognosis in Chinese is still uncertain. Real-time quantitative reverse transcription PCR (qRT-PCR) was employed in this study to compare the relative expression of miR-21 between pre-operative and post-operative paired plasmas from 42 patients with primary GCs. The results showed that the expression levels of miR-21 in the post-operative plasmas were significantly reduced by an average of 18.2 times in all patients when compared to the pre-operative plasmas, and by 22.1 times in the subgroup of patients without family history, while only 1.76 times in the subgroup of patients with a family history. With respect of clinicopathological characteristics, the plasma miR-21 expression was highly associated with differentiation degree and lymph node metastasis rate. The results suggested plasma miR-21 could be a novel potential biomarker for GC prognosis and evaluation of surgery outcomes, especially in patients without a family history.

Seismic behavior of energy dissipation shear wall with CFST column elements

Hao Su,Lihua Zhu,Yaohong Wang,Lei Feng,Zeyu Gao,Yuchen Guo,Longfei Meng,Hanquan Yuan 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.43 No.1

To develop high-efficiency lateral force resistance components for high-rise buildings, a novel energy dissipation shear wall with concrete-filled steel tubular (CFST) column elements was proposed. An energy dissipation shear wall specimen with CFST column elements (GZSW) and an ordinary reinforced concrete shear wall (SW) were constructed, and experimented by low-cycle reversed loading. The mechanical characteristics of these two specimens, including the bearing capacity, ductility, energy dissipation, and stiffness degradation process, were analyzed. The finite-element model of the GZSW was established by ABAQUS. Based on this finite-element model, the effect of the placement of steel-plate energy dissipation connectors on the seismic performance of the shear wall was analyzed, and optimization was performed. The experiment results prove that, the GZSW exhibited a superior seismic performance in terms of bearing capacity, ductility, energy dissipation, and stiffness degradation, in comparison with the SW. The results calculated by the ABAQUS finite-elements model of GZSW corresponded well with the results of experiment, and it proved the rationality of the established finite-elements model. In addition, the optimal placement of the steel-plate energy dissipation connectors was obtained by ABAQUS.

Inhibition of lncRNA KCNQ1OT1 Improves Apoptosis and Chemotherapy Drug Response in Small Cell Lung Cancer by TGF-β1 Mediated Epithelial-to-Mesenchymal Transition

Deyu Li,Qin Tong,Yuane Lian,Zhizhong Chen,Yaru Zhu,Weimei Huang,Yang Wen,Qiongyao Wang,Shumei Liang,Man Li,Jianjing Zheng,Zhenhua Liu,Huanxin Liu,Linlang Guo 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose Drug resistance is one of the main causes of chemotherapy failure in patients with small cell lung cancer (SCLC), and extensive biological studies into chemotherapy drug resistance are required. Materials and Methods In this study, we performed lncRNA microarray, in vitro functional assays, in vivo models and cDNA microarray to evaluate the impact of lncRNA in SCLC chemoresistance. Results The results showed that KCNQ1OT1 expression was upregulated in SCLC tissues and was a poor prognostic factor for patients with SCLC. Knockdown of KCNQ1OT1 inhibited cell proliferation, migration, chemoresistance and promoted apoptosis of SCLC cells. Mechanistic investigation showed that KCNQ1OT1 can activate transforming growth factor-β1 mediated epithelial-to-mesenchymal transition in SCLC cells. Conclusion Taken together, our study revealed the role of KCNQ1OT1 in the progression and chemoresistance of SCLC, and suggested KCNQ1OT1 as a potential diagnostic and prognostic biomarker in SCLC clinical management.

Dynamic changes in carbohydrate metabolism and endogenous hormones during Tulipa edulis stolon development into a new bulb

Yuanyuan Miao,Zaibiao Zhu,Qiaosheng Guo,Xiaohua Yang,Li Liu,Yuan Sun,Changlin Wang 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

The stolon is the main asexual reproductive organ of Tulipa edulis (Miq.) Baker. It has a special morphology and can develop into a new bulb for propagation. In the current greenhouse experiment, the dynamic changes in carbohydrates and related enzymes, protein and endogenous hormones during T. edulis stolon development were investigated. The results showed that soluble sugar levels were basically declining, whereas starch and protein content rose continuously during stolon development. The adenosine diphosphoglucose pyrophosphorylase (AGPase) activity peaked in the initial swelling stage and stayed a relative high level in the middle swelling stage; sucrose synthase (SS), soluble starch synthase (SSS) and granule-bound starch synthase (GBSS) activities followed the same law that showed rising trends during stolon development. SS activity was significantly inversely related to sucrose content but had significantly positive relations with starch content, SSS and GBSS activities. Gibberellin (GA), indole-3-acetic acid (IAA) and zeatin riboside (ZR) peaked in the initial swelling stage and maintained high levels in the middle swelling stage; they then decreased significantly in the later swelling stage. A substantial increase was observed in abscisic acid (ABA) content until the middle swelling stage, followed by a significant reduction in the later swelling stage. The ratios of ABA to IAA, GA and ZR reached their lowest levels in the initial swelling stage. In conclusion, T. edulis stolon development is a process of new bulb morphogenesis along with the starch accumulation catalyzed by AGPase, SSS and GBSS, using the product of sucrose cleavage caused by SS. Initial low ABA content and low ratios of ABA to IAA, GA and ZR, together with the GA, IAA and ZR of high-content, soluble sugars worked more efficiently to induce new bulb formation.

An Experimental Model for Induction of Lung Cancer in Rats by Chlamydia Pneumoniae

Chu, De-Jie,Guo, Shui-Gen,Pan, Chun-Feng,Wang, Jing,Du, Yong,Lu, Xu-Feng,Yu, Zhu-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Objective: To assess induction effects of Chlamydia pneumoniae (Cpn) on lung cancer in rats. Methods: A lung cancer animal model was developed through repeated intratracheal injection of Cpn (TW-183) into the lungs of rats, with or without exposure to benzo(a)pyrene (Bp). Cpn antibodies (Cpn-IgA, -IgG, and -IgM) in serum were measured by microimmunofluorescence. Cpn-DNA or Cpn-Ag of rat lung cancer was detected through polymerase chain reaction or enzyme-linked immunosorbent assay. Results: The prevalence of Cpn infection was 72.9% (35/48) in the Cpn group and 76.7% (33/43) in the Cpn plus benzo(a)pyrene (Bp) group, with incidences of lung carcinomas in the two groups of 14.6% (7/48) and 44.2% (19/43), respectively (P-values 0.001 and <0.000 compared with normal controls). Conclusions: A rat model of lung carcinoma induced by Cpn infection was successfully established in the laboratory for future studies on the treatment, prevention, and mechanisms of the disease.