Soil Phosphorus Availability of Plantation in Degraded Sub-Tropical Hilly Red Soil Region

Xia Gong,Xiaohua Wei,Xiaorui Zhao,Yuanqiu Liu,Dekui Niu,Wenyuan Zhang,Dongnan Hu,Zhi Li,Xiaomin Guo 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Improved Expression of Muscle-derived Growth Hormone Releasing Hormone from ${\alpha}$-Skeletal-muscle Actin Enhancer/Cytomegalovirus Hybrid Enhancer/Promoter

Gong, Xia,Meng, Qingyong,Jin, Weiwu,Li, Ning Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5

Growth hormone-releasing hormone (GHRH), a hypothalamic neuropeptide can stimulate the growth hormone secretion from the anterior pituitary. In this study, a porcine GHRH expression plasmid pHC-GHRH was used to enhance growth performance through ectopic expressions in muscle tissues of rats. Rats injected with the plasmid of pHC-GHRH and pCMV-GHRH exhibited cumulative weight gains 6.4% and 1% greater than controls. During a 5-day period, significant weight gain differences were observed as follows compared with that of control: during 5-10 days post-injection (DPI) period, the group pHC-GHRH on average 14.5% heavier than controls, $40.73{\pm}0.88$ g vs. $35.57{\pm}1.23$ g (p = 0.0023); during 10-15 DPI period, the group pHC-GHRH on average 13.6% heavier than controls, $37.49{\pm}2.85$ g vs. $33.00{\pm}1.56$ g (p = 0.0146); during 15-20 DPI period, the group pHC-GHRH on average 17.8% heavier than controls, $25.64{\pm}1.39$ g vs. $21.77{\pm}1.27$ g (p<0.05). In addition, plasmids-treated rats maintained higher serum IGF-I than controls. Significant differences of IGF-I were observed on 13 DPI and on 40 DPI in pHC-GHRH group compared with that of controls. This was accomplished through the use of an improved expression cassette that included the cytomegalovirus (CMV) immediate early enhancer/promoter in combination with a 1.5-kilobase portion of porcine ${\alpha}$-skeletal muscle actin promoter.

Effects of tryptophan and phenylalanine on tryptophol production in Saccharomyces cerevisiae revealed by transcriptomic and metabolomic analyses

Gong Xiaowei,Luo Huajun,Hong Liu,Wu Jun,Wu Heng,Song Chunxia,Zhao Wei,Han Yi,Dao Ya,Zhang Xia,Zhu Donglai,Luo Yiyong 한국미생물학회 2022 The journal of microbiology Vol.60 No.8

Tryptophol (TOL) is a metabolic derivative of tryptophan (Trp) and shows pleiotropic effects in humans, plants and microbes. In this study, the effect of Trp and phenylalanine (Phe) on TOL production in Saccharomyces cerevisiae was determined, and a systematic interpretation of TOL accumulation was offered by transcriptomic and metabolomic analyses. Trp significantly promoted TOL production, but the output plateaued (231.02−266.31 mg/L) at Trp concentrations ≥ 0.6 g/L. In contrast, Phe reduced the stimulatory effect of Trp, which was strongly dependent on the Phe concentration. An integrated genomic, transcriptomic, and metabolomic analysis revealed that the effect of Trp and Phe on TOL production was mainly related to the transamination and decarboxylation of the Ehrlich pathway. Additionally, other genes, including thiamine regulon genes (this), the allantoin catabolic genes dal1, dal2, dal4, and the transcriptional activator gene aro80, may play important roles. These findings were partly supported by the fact that the thi4 gene was involved in TOL production, as shown by heterologous expression analysis. To the best of our knowledge, this novel biological function of thi4 in S. cerevisiae is reported here for the first time. Overall, our findings provide insights into the mechanism of TOL production, which will contribute to TOL production using metabolic engineering strategies.

Functional Components of Bamboo Shavings and Bamboo Leaf Extracts and Their Antioxidant Activities In Vitro

Jinyan Gong,Dao-Zong Xia,Jun Huang,Qing Ge,Jian-wei Mao,Shiwang Liu,Ying Zhang 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.4

This study was designed to detect characteristic compounds and evaluate the free radical scavenging capacity of the bamboo leaves extract and bamboo shavings extract (BSE). The antioxidant capacity of bamboo leaf n-butanol fraction (AOB) exhibited the highest total phenolic content (49.93%), total flavonoids content (24.11%), and characteristic flavonoids and phenolic acids, such as chlorogenic acid, caffeic acid, ferulic acid, p-coumaric acid, orientin, homoorientin, vitexin, and isovitexin. Available data obtained with in vitro models suggested that AOB had higher free radical scavenging capacity with IC50 values of 1.04, 4.48, 5.37, and 1.12 μg/mL on DPPH•, O2•- , •OH, and H2O2, respectively, than the other two extracts, bamboo leaf water extract and BSE. The results indicated that the extracts from different parts of the bamboo possess excellent antioxidant activity, which can be used potentially as a readily accessible and valuable bioactive source of natural antioxidants.

Human SNF2L Gene Is Regulated Constitutively and Inducibly in Neural Cells via a cAMP-Response Element

Yu Xia,Laicheng Wang,Chunyan Ma,Yaoqin Gong,Yueran Zhao 연세대학교의과대학 2013 Yonsei medical journal Vol.54 No.3

Purpose: SNF2L belongs to Imitation Switch family and plays an essential role in neural tissues and gonads. In our previous studies, we have demonstrated that the basal transcription of human SNF2L gene is regulated by two cis-elements, cAMP response element (CRE)- and Sp1-binding sites. Recent studies suggested that cyclic adenosine monophosphate (cAMP) stimulation significantly up-regulated SNF2L expression in ovarian granulose cells. These data suggested that protein kinase-mediated signal pathways might also regulate SNF2L expression in neural cells. We therefore investigated the effects of agents that activate protein kinases A on SNF2L gene expression in neural cells. Materials and Methods: To increase intracellular cAMP levels, all neural cells were treated with forskolin and dbcAMP, two cAMP response activators. We exmined the effects of cAMP on the promoter activity of human SNF2L gene by luciferase reporter gene assays, and further examined the effects of cAMP on endogenous SNF2L mRNA levels by qPCR. Results: Transient expression of a luciferase fusion gene under the control of the SNF2L promoter was significantly increased by treatment of rat primary neurons with forskolin or dbcAMP, but not PC12, C6 and SH-SY5Y cells. Consistently, treatment with forskolin or dbcAMP could enhance endogenous SNF2L mRNA levels also only in rat primary neurons. Conclusion: These results suggest that the CRE consensus sequence in the SNF2L proximal promoter most likely confers constitutive activation and regulation by cAMP in neural cells.

Geographic Distribution and Epidemiology of Lung Cancer During 2011 in Zhejiang Province of China

Lin, Xia-Lu,Chen, Yan,Gong, Wei-Wei,Wu, Zhao-Fan,Zou, Bao-Bo,Zhao, Jin-Shun,Gu, Hua,Jiang, Jian-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Background: To explore etiology for providing scientific clues for the prevention of lung cancer. Materials and Methods: Data for lung cancer incidence and meteorological geographic factors from 25 counties in Zhejiang province of China during 2011 were studied. Stepwise multiple regression and correlation analysis were performed to analyze the geographic distribution and epidemiology of lung cancer. Results: 8,291 new cases (5,998 in males and 2,293 females) of lung cancer during 2011 in Zhejiang province were reported in the 25 studied counties. Reported and standardized incidence rates for lung cancer were 58.0 and 47.0 per 100,000 population, respectively. The incidence of lung cancer increased with age. Geographic distribution analysis shows that the standardized incidence rates of lung cancer in northeastern Zhejiang province were higher than in the southwestern part, such as in Nanhu, Fuyang, Wuxing and Yuyao counties, where the rates were more than 50 per 100,000 population. In the southwestern Zhejiang province, for instance, in Yueqing, Xianju and Jiande counties, the standardized incidence rates of lung cancer were lower than 37 per 100,000 population. Spearman correlation tests showed that forest coverage rate, air quality index (AQI), and annual precipitation level are associated with the incidence of lung cancer. Conclusions: Lung cancer in Zhejiang province shows obvious regional differences. High incidence appears associated with low forest coverage rate, poor air quality and low annual precipitation. Therefore, increasing the forest coverage rate and controlling air pollution may play an important role in lung cancer prevention.

PSO-based Resource Allocation in Software-Defined Heterogeneous Cellular Networks

( Wenrong Gong ),( Lihua Pang ),( Jing Wang ),( Meng Xia ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.5

A heterogeneous cellular network (HCN) is useful to increase the spectral and energy efficiency of wireless networks and to reduce the traffic load from the macro cell. The performance of the secondary user equipment (SUE) is affected by interference from the eNodeB (eNB) in a macro cell. To decrease the interference between the macro cell and the small cell, allocating resources properly is essential to an HCN. This study considers the scenario of a software-defined heterogeneous cellular network and performs the resource allocation process. First, we show the system model of HCN and formulate the optimization problem. The optimization problem is a complex process including power and frequency resource allocation, which imposes an extremely high complexity to the HCN. Therefore, a hierarchical resource allocation scheme is proposed, which including subchannel selection and a particle swarm optimization (PSO)-based power allocation algorithm. Simulation results show that the proposed hierarchical scheme is effective in improving the system capacity and energy efficiency.

A Stochastic Frontier Analysis of Trade Efficiency for the Sino-Korea Trade

Wen-Chao Gong,Kanyong Li,Wen-Xia Wang 한국무역학회 2022 Journal of Korea trade Vol.26 No.1

Purpose – This paper intends to make theoretical analysis and empirical test on the factors influencing China's export to South Korea, and draw conclusions about China's export efficiency and trade potential. Based on the conclusions, the reasons for China's trade deficit with South Korea are found, and a solution is put forward for solving the problem of China's trade deficit with South Korea. Design/methodology – Based on the data of 2004-2017 years in China, this paper uses the stochastic frontier gravity model to analyze the influencing factors of China's export to South Korea, as well as the export efficiency of each province and the export potential that can be explored. Findings – First, in terms of the factors affecting China's export trade to South Korea, the GDP of the provinces and cities in China, the FDI of South Korea to the provinces and cities in China, the GDP of South Korea, the population and education level of provinces and cities in China can significantly promote the export scale of Chinese provinces and cities to South Korea. The distance between Chinese provincial capitals and the South Korean capital significantly hinders Chinese exports to South Korea; Second, in terms of export trade efficiency, the trade exchange rate of the economically developed cities along the eastern coast of China and several provinces that are close to South Korea is higher than that of the cities in the central and western regions; Third, economic globalization makes trade more convenient, the average export trade efficiency of China's exports to South Korea showed an upward trend. However, under the influence of the 2008 global financial crisis, the export trade efficiency declined from 2008 to 2009, indicating that the impact of the financial crisis on the trade efficiency cannot be ignored. Originality/value – This paper finds out the influencing factors of China's export to South Korea, analyzes the export efficiency of different provinces and cities, excavates the export potential, and puts forward some suggestions for the balanced development of China and South Korea trade in the next step.

Synthesis and negative inotropic effects evaluation of 7-substituted-4,5-dihydro-[1,2,4]oxadiazolo[4,3-a]quinolin- 1-ones

Lan Hong,Guo-Hua Gong,Li Yu,Ming-Xia Song,Xun Cui,Zhe-Shan Quan 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.11

A series of 7-alkoxy-4,5-dihydro-[1,2,4]oxadiazolo[4,3-a]quinolin-1-ones was synthesized and theirnegative inotropic effects were evaluated by measuring theleft atrium stroke volume in isolated rabbit heart preparations. All compounds moderated the cardiac workload bydecreasing heart rate and contractility (inotropic effects). Among them, compound 6 was found to be best potent witha -28.89 ± 1.91 % decrease in the stroke volume at aconcentration of 3 9 10-5 M in our in vitro study.

Exploring the Factors Affecting Behavioral Intentions of Spectators: Case Study of 2019 FIBA Basketball World Cup

( Shihao Huang ),( Yinggang Chen ),( Xia Xu ),( Yingying Gong ),( Bin Xu ) 아시아스포츠융합과학회 2021 Asia Pacific Journal of Applied Sport Sciences Vol.2 No.3

PURPOSE In this study, The event-destination image transfer model was used to predict and test the behavioral intention of spectators watching major sports events. Based on The Event-destination image transfer model, this study added place attachment as a new variable to investigate The influencing factors of audience decision-making process. METHOD This study sampled 500 spectators of the 2019 FIBA Basketball World Cup held in Beijing, China. The 2019 FIBA Basketball World Cup was held in Beijing, Guangzhou, Nanjing, Shanghai, Wuhan, Shenzhen, Foshan and Dongguan on September 15, 2019. RESULT After spectators attend a mega-event held in a tourism destination, the event image positively affected the destination image. Destination image directly and positively affected spectators' behavioral intentions toward the destination. Tourists' overall attitude toward the destination was influenced positively by both the destination image and event image. CONCLUSION There are three significant antecedents factors for the behavioral intention of the destination, namely the image of the destination, tourists' overall attitude towards the destination and place attachment. The destination image directly and positively affects place attachment. Place attachment directly and positively affects tourists' behavioral intentions toward the destination.