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        Variability of matrix effects in liquid and gas chromatography-mass spectrometry analysis of pesticide residues after QuEChERS sample preparation of different food crops

        Kwon, H.,Lehotay, S.J.,Geis-Asteggiante, L. Elsevier 2012 Journal of Chromatography A Vol.1270 No.-

        Gas and liquid chromatography (GC and LC) coupled to mass spectrometry (MS) serve as the most powerful analytical tools commonly used to monitor pesticide residues in food, among other applications. However, both GC-MS and LC-MS are susceptible to matrix effects which can adversely affect quantification depending on the analyte, matrix, sample preparation, instrumentation, and operating conditions. Among the approaches that reduce matrix effects, the most common in pesticide residue applications is matrix-matched calibration because it is relatively inexpensive and simple. Also, it has been shown to work well during method validation when fortified samples are exactly matched with samples used for calibration. However, the quality of matrix-matched results in real-world analyses depends on the consistency of matrix effects among diverse samples. In this study, the variability of matrix effects was measured for 38 representative pesticides in 20 samples each (including different varieties) of rice, orange, apple, and spinach extracted using the ''quick, easy, cheap, effective, rugged, and safe'' (QuEChERS) method for analysis by LC-MS/MS and low-pressure GC-MS. Using LC-MS/MS, only oranges gave >20% matrix effects for a few pesticides. GC-MS exhibited larger matrix effects, but as in LC-MS/MS, the differences were reasonably consistent among the 20 samples tested. Main conclusions of this study are that for the conditions utilized: (1) matrix-matching was not needed for most pesticides in the simpler food matrices; and (2) for the more complex orange matrix, acceptably accurate quantitative results were achieved by using matrix-matching even with a different sample of the same type. However, full confidence cannot be extended to matrix-matched results, and for consequential applications such as regulatory enforcement, confirmatory analyses using alternate quantitative determinations should also be conducted.

      • SCISCIESCOPUS

        HIV-1 Genetic Diversity Among Incident Infections in Mbeya, Tanzania

        Billings, Erik,Sanders-Buell, Eric,Bose, Meera,Kijak, Gustavo H.,Bradfield, Andrea,Crossler, Jacqueline,Arroyo, Miguel A.,Maboko, Leonard,Hoffmann, Oliver,Geis, Steffen,Birx, Deborah L.,Kim, Jerome H. MARY ANN LIEBERT INC PUBL 2017 AIDS RESEARCH AND HUMAN RETROVIRUSES Vol.33 No.4

        <P><B>Abstract</B></P><P>In preparation for vaccine trials, HIV-1 genetic diversity was surveyed between 2002 and 2006 through the Cohort Development study in the form of a retrospective and prospective observational study in and around the town of Mbeya in Tanzania's Southwest Highlands. This study describes the molecular epidemiology of HIV-1 strains obtained from 97 out of 106 incident HIV-1 infections identified in three subpopulations of participants (one rural, two urban) from the Mbeya area. Near full-genome or half-genome sequencing showed a subtype distribution of 40% C, 17% A1, 1% D, and 42% inter-subtype recombinants. Compared to viral subtyping results previously obtained from the retrospective phase of this study, the overall proportion of incident viral strains did not change greatly during the study course, suggesting maturity of the epidemic. A comparison to a current Phase I-II vaccine being tested in Africa shows ∼17% amino acid sequence difference between the gp120 of the vaccine and subtype C incident strains. Phylogenetic and recombinant breakpoint analysis of the incident strains revealed the emergence of CRF41_CD and many unique recombinants, as well as the presence of six local transmission networks most of which were confined to the rural subpopulation. In the context of vaccine cohort selection, these results suggest distinct infection transmission dynamics within these three geographically close subpopulations. The diversity and genetic sequences of the HIV-1 strains obtained during this study will greatly contribute to the planning, immunogen selection, and analysis of vaccine-induced immune responses observed during HIV-1 vaccine trials in Tanzania and neighboring countries.</P>

      • 보건계열 중 간호과와 안경광학과 학생들의 학습 성향 분석

        최계훈,소명숙 대구보건전문대학교 2006 대구보건대학 論文集 Vol.26 No.-

        The lst grade of Nur (Department of Nursing), ACT was higher than REF, but ACT was lower than REF in the 2nd and 3rd grade. SEN was increased in the 2nd grade more than lst grade, but it was decreased in the 3rd grade, VIS as well. The case of SEQ, it developed a tendency to increase as their grade was higher. The Oph (Department of Ophthalmic Optics) showed a tendency to REF, but it changed to propensity of ACT in the 2nd grade. And also propensity of SEN was much higher than INT when they were in the lst grade, but the gap was reduced when they were 2nd grade. As the grade was higher, the students had preference of VIS over VRB and the gap of the preference between VIS and VRB was increased, the propensity of SEQ was increased as well. Therefore the first students in the Department of Nursing showed strong propensity of ACT and they changed to the propensity of REF after. The students of the Department of Ophthalmic Optics showed the opposite propensity. The other learning propensity showed that the propensity of the first grade maintained to upper grade.

      • 커패시터 필터를 갖는 3 상 다이오드 정류회로의 불평형전원에서의 입력전류 특성

        이동기(Dong-Gei Lee),박기원(Gei won Park),정승기(Seong Gei Jung) 전력전자학회 2001 전력전자학술대회 논문집 Vol.2001 No.7

        The three-phase diode rectifier with capacitive filter is highly-sensitive to line voltage unbalance. Because of its inherent nonlinear characteristics, small line voltage unbalance may cause highly unbalanced line current, causing detrimental effects on power quality This paper presents a theoretical basis on this 'unbalance amplification effect' and derives an analytical model of line current characteristics under unbalanced line voltage condition for various modes of operatioa The results provide a basic and important guideline for optimal design of a three-phase diode rectifier with capacitive filter that is most commonly used for interfacing various power conversion equipments to power lines.

      • KCI등재
      • The Detection Model of Malignant Query and Personal Information Leakage based on Log Analysis

        Gei-Young Kim,Kyung-Jin Jung,Yongtae Shin,Sangphil Kim,Jong-Bae Kim 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.11

        Many behaviors happen in information protection control, threatening from unauthorized change, destruction, and exposure to integrity, confidentiality, and availability of database, which is the final and core object of control. Like this it approaches database through numerous paths like many applications and home pages and execute query which search, modify, and delete the data. Some of it executes normal queries, but sometimes it maliciously executes the queries for leakage of information, and gives load to database server by executing the query which uses large amount of hardware resources. Traditionally it has limits, using only to find the reason for the problems, such as malignant queries, by collecting security log. Analyzing malignant queries and personal information leakage in diversified views through multidimensional analysis of data is necessary in order to use security log in more various ways. Therefore, this treatise is going to design multidimensional analysis modeling and suggest the technology to analyze in diversified views as an application plan of existing security log so that we can detect malignant queries and personal information leakage through security log analysis. We established the standard of analysis as follows for various analyses. First, we made linkage analysis available, which we cannot know with only simple history search, through analysis of database examination history. Second, we analyze if it repeatedly approached important table for a long time through detection of abnormal pattern or long term leakage via database abnormal access analysis. Third, we understood the flow of elements and data which weigh impact on specific database assets through database impact analysis and made analysis of database assets correlation and data flow analysis available. For analysis this treatise analyzed the log collected by using OLAP tools and used experiment data and operation data in order to verify the efficiency of database security log analysis technology suggested. Also we showed that the analysis method suggested by this treatise is excellent in availability and credibility in detection of malignant queries and personal information leakage, by comparing traditional data analysis method and the analysis method suggested by this treatise.

      • KCI등재

        Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides

        Geise Camila de Araujo Ribeiro,Pedro Fernandes,Dayse Alessandra Almeida Silva,Hugo Neves Brandao,Sandra Aparecida de Assis 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.7

        The crude extract containing inulinase fromRhodotorula mucilaginosa was obtained by submergedfermentation. Inulinase was immobilized on chicken eggshellby physical adsorption and covalent crosslinking,using glutaraldehyde as a crosslinking reagent, and Celiteby adsorption. Fructooligosaccharides production wasperformed using immobilized inulinase (5%, w/v) andinulin substrate solution under experimental conditionsevaluated through Doehlert experimental design. The productionof inulinase was optimized for concentrations ofD-glucose and yeast extract at 12.5 and 0.5 g/L, respectively,resulting in an optimal activity of 0.62 U. Theoptimal pH and temperature for enzyme activity were 8.0and 75 C, respectively, leading to an optimal activity of3.54 U. The highest immobilization efficiency (46.27%)was obtained upon immobilization on Celite. Immobilizationby adsorption to eggshell allowed for specific activityof 4.15 U/g, and adsorption to Celite resulted in specificactivity of 3.70 U/g. The highest titer in fructooligosaccharideswas obtained with an initial inulin concentrationof 250 g/L (25%, w/v), and a reaction time of 16 h. Hence,immobilized inulinase proved to be a promising catalyst forfructooligosaccharides production since the formulation isperformed through a simple, low-cost, and large-scaleapplicable methodology.

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