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( Yi Han Liu ),( Shu Ai Fan ),( Xiao Guang Liu ),( Zhi Meng Zhang ),( Jian Ling Wang ),( Zheng Xiang Wang ),( Fu Ping Lu ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.7
The stability of Bacillus licheniformis alpha-amylase (BLA) under acid condition was enhanced through direct evolution using the error-prone polymerase chain reaction. One beneficial mutation site, H281I, was obtained in BLA. The specific activity of H281I was 161/352 U/mg, which was 62.6/27.5% higher than that of the wild-type (WT) (99/276 U/mg) at pH 4.5/6.5 and 95°C. The pH optimum for H281I was decreased about 1 unit, whereas no significant changes of optimum temperature and thermostability were observed compared with the wild type (WT). The kcat/Km value of H281I was 1.7-/1.4-fold higher at pH 4.5/6.5, respectively, than that of WT. The structure model analysis indicated that the H281I mutation altered the predicted interaction between the amino acid residues at 281 and 273, thus creating a conducive local environment for substrate binding, as reflected by its decreased Km, and consequently increased the specific activity.
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( Ping Sheng ),( Yun-long Cheng ),( Chuan-chuan Cai ),( Wei-jin Guo ),( Ying Zhou ),( Ge Shi ),( Yi-ming Fan ) 대한피부과학회 2016 Annals of Dermatology Vol.28 No.6
Background: Although Becker`s nevus (BN) is a relatively common disease, the systematic studies of clinicopathological and immunohistochemical results are poorly reported. Objective: To investigate the clinicopathological features and immunohistochemical alterations of keratinocyte proliferation, melanocyte density, smooth muscle hyperplasia and nerve fiber distribution in BN. Methods: Clinical and pathological data were collected in 60 newly-diagnosed BN cases. Immunohistochemical stain of Ki-67, Melan-A, keratin 15, smooth muscle actin and protein gene product 9.5 was performed in 21 cases. Results: The median diagnostic and onset age was 17 and 12 years, respectively. Skin lesions usually appeared on the upper trunk and upper limbs. The pathological features included the rete ridge elongation and fusion and basal hyperpigmentation. Epidermal Ki-67, Melan-A and keratin 15 expression and dermal nerve fiber length were significantly higher in lesional and perilesional skin than in normal skin (p<0.05∼0.01), while smooth muscle actin expression was upregulated only in skin lesion (p<0.05). Conclusion: Although the clinical diagnosis of BN is often straightforward, histopathology is helpful to differentiate from other pigmentary disorders. The hyperproliferation of keratinocytes, melanocytes, arrector pili muscle and dermal nerve fibers could be involved in the patho-genesis of BN. (Ann Dermatol 28(6) 697∼703, 2016)
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Near-Five-Vector SVPWM Algorithm for Five-Phase Six-Leg Inverters under Unbalanced Load Conditions
Ping Zheng,Pengfei Wang,Yi Sui,Chengde Tong,Fan Wu,Tiecai Li 전력전자학회 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.1
Multiphase machines are characterized by high power density, enhanced fault-tolerant capacity, and low torque pulsation. For a voltage source inverter supplied multiphase machine, the probability of load imbalances becomes greater and unwanted low-order stator voltage harmonics occur. This paper deals with the PWM control of multiphase inverters under unbalanced load conditions and it proposes a novel near-five-vector SVPWM algorithm based on the five-phase six-leg inverter. The proposed algorithm can output symmetrical phase voltages under unbalanced load conditions, which is not possible for the conventional SVPWM algorithms based on the five-phase five-leg inverters. The cause of extra harmonics in the phase voltages is analyzed, and an xy coordinate system orthogonal to the ab z coordinate system is introduced to eliminate low-order harmonics in the output phase voltages. Moreover, the digital implementation of the near-five-vector SVPWM algorithm is discussed, and the optimal approach with reduced complexity and low execution time is elaborated. A comparison of the proposed algorithm and other existing PWM algorithms is provided, and the pros and cons of the proposed algorithm are concluded. Simulation and experimental results are also given. It is shown that the proposed algorithm works well under unbalanced load conditions. However, its maximum modulation index is reduced by 5.15% in the linear modulation region, and its algorithm complexity and memory requirement increase. The basic principle in this paper can be easily extended to other inverters with different phase numbers.
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Near-Five-Vector SVPWM Algorithm for Five-Phase Six-Leg Inverters under Unbalanced Load Conditions
Zheng, Ping,Wang, Pengfei,Sui, Yi,Tong, Chengde,Wu, Fan,Li, Tiecai The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.1
Multiphase machines are characterized by high power density, enhanced fault-tolerant capacity, and low torque pulsation. For a voltage source inverter supplied multiphase machine, the probability of load imbalances becomes greater and unwanted low-order stator voltage harmonics occur. This paper deals with the PWM control of multiphase inverters under unbalanced load conditions and it proposes a novel near-five-vector SVPWM algorithm based on the five-phase six-leg inverter. The proposed algorithm can output symmetrical phase voltages under unbalanced load conditions, which is not possible for the conventional SVPWM algorithms based on the five-phase five-leg inverters. The cause of extra harmonics in the phase voltages is analyzed, and an xy coordinate system orthogonal to the ${\alpha}{\beta}z$ coordinate system is introduced to eliminate low-order harmonics in the output phase voltages. Moreover, the digital implementation of the near-five-vector SVPWM algorithm is discussed, and the optimal approach with reduced complexity and low execution time is elaborated. A comparison of the proposed algorithm and other existing PWM algorithms is provided, and the pros and cons of the proposed algorithm are concluded. Simulation and experimental results are also given. It is shown that the proposed algorithm works well under unbalanced load conditions. However, its maximum modulation index is reduced by 5.15% in the linear modulation region, and its algorithm complexity and memory requirement increase. The basic principle in this paper can be easily extended to other inverters with different phase numbers.
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Qin Ping Yu,Ding Yuan Feng,Juan Xiao,Fan Wu,Xiao Jun He,Min Hao Xia,Tao Dong,Yi Hua Liu,Hui Ze Tan,Shi Geng Zou,Tao Zheng,Xian Hua Ou,Jian Jun Zuo 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.12
Objective: This experiment investigated meat color, myoglobin content, enzyme activities, and expression of genes associated with oxidative potential of pigs slaughtered at different growth stages. Methods: Sixty 4-week-old Duroc×Landrace×Yorkshire pigs were assigned to 6 replicate groups, each containing 10 pigs. One pig from each group was sacrificed at day 35, 63, 98, and 161 to isolate longissimus dorsi and triceps muscles. Results: Meat color scores were higher in pigs at 35 d than those at 63 d and 98 d (p<0.05), and those at 98 d were lower than those at 161 d (p<0.05). The total myoglobin was higher on 161 d compared with those at 63 d and 98 d (p<0.05). Increase in the proportions of metmyoglobin and deoxymyoglobin and a decrease in oxymyoglobin were observed between days 35 and 161 (p<0.05). Meat color scores were correlated to the proportion of oxymyoglobin (r = 0.59, p<0.01), and negatively correlated with deoxymyoglobin and metmyoglobin content (r = –0.48 and –0.62, p<0.05). Malate dehydrogenase (MDH) activity at 35 d and 98 d was higher than that at 161 d (p<0.05). The highest lactate dehydrogenase/MDH ratio was achieved at 161 d (p<0.05). Calcineurin mRNA expression decreased at 35 d compared to that at 63 d and 98 d (p<0.05). Myocyte enhancer factor 2 mRNA results indicated a higher expression at 161 d than that at 63 d and 98 d (p<0.05). Conclusion: Porcine meat color, myoglobin content, enzyme activities, and genes associated with oxidative potential varied at different stages.
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Wang, Yi-Chang,Fan, Ping-Chin INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1972 YONSEI REPORTS ON TROPICAL MEDICINE Vol.3 No.1
O. H. chiui infected with either Formosan(Changhea) or Philippine strain of S. japonicum were divided into three series to submerge into dechlorinated tap water in 5-ml vials at 7 a.m., 3p.m., and 11 p.m. of theday of experiment, then they were examined 2-hourly when exposed either to continuously fluorescent light or to day light and darkness at night for 40 hours to datermine the sequence of their cercarial emergence. Results showed a afternon (3∼11p.m.) cycle for cercariae of Philippine strain of S. japonicum and a tendency of diurnal periodicity for cercariae of Formosan (Changhua) strain of S. japonicum to emerge from O. h. chiui. After snails of O.h. chiui wer submerged in water, s. japonicum cercariae of Formosan (Changhua) strain were much earlier than those of Philippine strain to emerge from their snail hossts. Light sstimulated cercariae of both Formosan(Changhua) strain and Philipoppine strain of S. japonicum carlier to emerge from their snail hosts. Factors might affect cercarial shedding of S. japonicum were also discussed in the present aper.
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Yu, Qin Ping,Feng, Ding Yuan,He, Xiao Jun,Wu, Fan,Xia, Min Hao,Dong, Tao,Liu, Yi Hua,Tan, Hui Ze,Zou, Shi Geng,Zheng, Tao,Ou, Xian Hua,Zuo, Jian Jun Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.11
Objective: This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula's extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of myosin heavy chain (MyHC) gene expression in myocytes. Methods: In a completely randomized design, ninety pigs were assigned to three diets with five replications per treatment and six pigs per pen. The diets included the basal diet (control group), TCMF1 (basal diet+2.5 g/kg TCMF) and TCMF2 (basal diet+5 g/kg TCMF). The psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining. Cell proliferation was measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye and cytometry. Isoforms of MyHC gene expression were detected by real-time quantitative polymerase chain reaction. Results: The final body weight and carcass weight of finishing pigs were increased by TCMF1 (p<0.05), while the psoas major muscle cross-sectional area was increased by TCMF (p<0.05). The cross-sectional area and diameter of psoas major muscle fiber Ι, IIA, and IIB were increased by TCMF2 (p<0.05). The cross-sectional area and fiber diameter of psoas major muscle fiber IIA and IIB were increased by diet supplementation with TCMF1 (p<0.05). Psoas major muscle fiber IIA and IIB fiber density from the pigs fed the TCMF1 diet and the type IIB fiber density from the pigs fed the TCMF2 diet were lower compared to pigs fed the control diet (p<0.05). Pigs fed TCMF2 had a higher composition of type Ι fiber and a lower percentage of type IIB fiber in the psoas major muscle (p<0.05). The expression levels of MyHC Ι, MyHC IIa, and MyHC IIx mRNA increased and the amount of MyHC IIb mRNA decreased in the psoas major muscle from TCMF2, whereas MyHC Ι and MyHC IIx mRNA increased in the psoas major muscle from TCMF1 (p<0.05). Peroxisome proliferator-activated receptor ${\gamma}$ $coactivator-1{\alpha}$ and CaN mRNA expression in the psoas major muscle were up-regulated by TCMF (p<0.05). Porcine skeletal muscle satellite cell proliferation was promoted by $4{\mu}g/mL$ and $20{\mu}g/mL$ TCMF water extraction (p<0.05). Both $1{\mu}g/mL$ and $5{\mu}g/mL$ of TCMF water extraction increased MyHC IIa, MyHC IIb, and MyHC IIx mRNA expression in porcine myocytes (p<0.05), while MyHC Ι mRNA expression in porcine myocytes was decreased by $5{\mu}g/mL$ TCMF water extraction (p<0.05). Porcine myocyte MyHC Ι and MyHC IIx mRNA expression were increased, and MyHC IIa and MyHC IIb mRNA expression were down-regulated by $5{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). MyHC Ι and MyHC IIa mRNA expression in porcine myocytes were increased, and the MyHC IIb mRNA expression was decreased by $1{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). Four isoforms of MyHC mRNA expression in porcine myocytes were reduced by $5{\mu}g/mL$ TCMF petroleum ether extraction (p<0.05). MyHC IIa mRNA expression in porcine myocytes increased and MyHC IIb mRNA expression decreased by $1{\mu}g/mL$ in a TCMF petroleum ether extraction (p<0.05). Conclusion: These results indicated that TCMF amplified the psoas major muscle cross-sectional area through changing muscle fiber characteristics in finishing pigs. This effect was confirmed as TCMF extraction promoted porcine cell proliferation and affected isoforms of MyHC gene expression in myocytes.
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