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Ferritin-Based New Magnetic Force Microscopic Probe Detecting 10 nm Sized Magnetic Nanoparticles
Kim, Duckhoe,Chung, Nak-Kwan,Allen, Stephanie,Tendler, Saul J. B.,Park, Joon Won American Chemical Society 2012 ACS NANO Vol.6 No.1
<P>A single-molecule ferritin picking-up process was realized with the use of AFM, which was enhanced by employing controlled dendron surface chemistry. The approach enabled the placement of a single ferritin protein molecule at the very end of an AFM tip. When used for magnetic force microscopy (MFM) imaging, the tips were able to detect magnetic interactions of approximately 10 nm sized magnetic nanoparticles. The single ferritin tip also showed the characteristics of a “multifunctional” MFM probe that can sense the magnetic force from magnetic materials as well as detect the biomolecular interaction force with DNAs on the surface. The multifunctional tip enabled us not only to investigate the specific molecular interaction but also to image the magnetic interaction between the probe and the substrate, in addition to allowing the common capability of topographic imaging. Because the protein engineering of ferritin and the supporting coordination and conjugation chemistry are well-established, we envisage that it would be straightforward to extend this approach to the development of various single magnetic particle MFM probes of different compositions and sizes.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2012/ancac3.2012.6.issue-1/nn203464g/production/images/medium/nn-2011-03464g_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn203464g'>ACS Electronic Supporting Info</A></P>
Immobilizing a single DNA molecule at the apex of AFM tips through picking and ligation
Kim, Duckhoe,Chung, Nak-Kwan,Kim, Jung Sook,Park, Joon Won Royal Society of Chemistry 2010 SOFT MATTER Vol.6 No.16
<P>A proper surface treatment of the substrate and the AFM tip with a structured molecule provided a new approach to pick a single DNA molecule with reasonable success rate (75%) by AFM. Picking a single molecule by AFM tip and realizing the single interaction will likely be useful for single molecule dynamic force spectroscopy, because it avoids the formation of problematic multiple interactions. As an extension, a new single DNA was allowed to hybridize with the picked DNA, and conjugated with the picker DNA by use of a ligase. The attachment of a single DNA at the apex was confirmed by measuring the force between the new DNA and the complementary DNA on the generic surface. Various applications of the tip for manipulating single molecules and preparing new nanomaterials are envisaged.</P> <P>Graphic Abstract</P><P>Single molecule picking up was enhanced by employing AFM tips and substrates modified with dendrons. The dendron modification guaranteed the single DNA–DNA interaction and realized the attachment of a single DNA at the apex of the tip. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c001722a'> </P>
김덕호(Duckho Kim),하재기(Jaekee Ha) 한국자동차공학회 2008 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-
Air pollution has recently become more serious, and the most critical factor for air pollution is the exhaust gas from diesel engines. It is proved that the exhaust gas in the air has bad influence on human body, so new technology has been developed to reduce the exhaust gas. Among the new technology an exhaust gas aftertreatment system is the most efficient way for exhaust gas, compared to the engine improvement in quality. In this study, trends of technology which reduces the exhaust gas mostly from diesel engines and prospects of future studies were investigated.
김덕호,하재기 김천과학대학 2007 김천과학대학 논문집 Vol.33 No.-
Air pollution has recently become more serious, and the most critical factor for air pollution isthe exhaust gas from diesel engines. It is proved that the exhaust gas in the air has bad influence on human body, so new technology has been developed to reduce the exhaust gas. Among the new technology an exhaust gas after treatment system is the most efficient way for exhaust gas, compared to the engine improvement in quality. In this study, trends of technology which reduces the exhaust gas mostly from diesel engines and prospects of future studies were investigated.
The Use of LIF-based Instrument with 405 nm for Real-time Monitoring of Aerosolized Bio-particles
Sung Nyo Yoon,Jaekyung Lee,Duckho Kim,Hyun Sang Yoo,Kyung Yool Min,Min Cheol Kim 한국대기환경학회 2019 Asian Journal of Atmospheric Environment (AJAE) Vol.13 No.3
Bio-aerosols can affect public health depending on the origin of bio-particles (bacteria, virus etc.). Here, we attempted to assess the applicability of laser-induced fluorescence (LIF) instrument with 405 nm to real-time monitoring of bacteria and viruscontaining aerosols. For the purpose, the LIF-based BDS (Bio-aerosol Detection System) was used. The bio-particle monitoring of the BDS is based on fluorescence signals from two wavelength ranges [short wavelength range (SWR): 430-550 nm & long wavelength range (LWR): 500-600 nm] and the scattering signal. Firstly, auto-fluorophores (NADH, riboflavin, tyrosine, tryptophan) were tested to expect the monitoring ranges of the BDS for the auto-fluorophores. NADH and riboflavin showed fluorescence signals from two wavelength ranges, and the fluorescence efficiency of NADH was higher in the SWR than in the LWR and that of riboflavin was reversed. While tyrosine and tryptophan showed negligible fluorescence signals from two wavelength ranges as expected. Next, the lyophilized powders of Bacillus subtilis (BS), virus vaccines [ND (Newcastle Disease), IB (Infectious Bronchitis)] and the bacteriophage MS2 were tested to investigate the monitoring ranges of the BDS for the bio-particles. Individual virus and bacteriophage have been expected no fluorescence signals because of the absence of NADH and riboflavin fluorescing by 405 nm. Nonetheless, all the tested samples showed the fluorescence signals in the size range of 2 to 15 μm, generally known as bio-aerosol size. Considering that atmospheric virus particles are released through the respiratory organs of their hosts, just as virus vaccines from chicken embryo and MS2 from E. coli, it can be thought in turn that the BDS can also monitor bio-aerosols including virus as well as bacteria. Taken together, we suggests that the BDS, LIF-based instrument with 405 nm, is applicable for real-time monitoring of virus-containing aerosols as well as other bio-aerosols by counting the fluorescence particles and resolving their particle sizes.