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Characterization of a Late Gene, ORF60 from Bombyx mori Nucleopolyhedrovirus
Du, Meng-Fang,Yin, Xin-Ming,Guo, Zhong-Jian,Zhu, Liang-Jun Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6
Open reading frame 60 of Bombyx mori nucleopolyhedrovirus (Bm60) is located between 56,673 and 57,479 bp in the BmNPV genome which encodes 268 amino acid residues with predicted molecular weight of 31.0 kDa. Bm60 and its homologues have been identified in 11 completely sequenced lepidopteran NPVs. The transcript of Bm60 was detected by RT-PCR at 18-72 h post-infection (p.i.), while the corresponding protein could be detected at 24-72 h p.i. in BmNPV-infected BmN cells by Western blot analysis using a polyclonal antibody against Bm60. The expression of Bm60 was inhibited in the presence of Ara-c, an inhibitor of viral DNA synthesis. These results together indicated that Bm60 was a late gene. The size of Bm60 product was found to be a 31 kDa in BmNPV-infected BmN cells, consistent with predicted molecular weight. Immuno-fluoresence analysis showed that the Bm60 product was first detected in the cytoplasm at 24 h p.i and also located in nucleus during later infection. In conclusion, the available data suggest that Bm60 is a functional ORF of BmNPV and encodes a 31kDa protein expressed in the later stage of infection cycle.
Ning-jun Du,Guo-liang Bai,Ya-zhou Xu,Chao-gang Qin 한국콘크리트학회 2017 International Journal of Concrete Structures and M Vol.11 No.3
Tubular reinforced concrete columns of air-cooling condenser structures, which undertake the most weight of air cooling equipment, are the major components to resist lateral forces under earthquake. Once collapsed, huge casualties and economic loss would be caused. Thus, four 1/8 scaled specimens were fabricated and tested through the pseudo-static testing method. Failure modes and crack patterns of the specimens under cyclic loading were observed. Then, finite element models of tubular reinforced concrete columns were established using OpenSees and were verified with the experimental results. Finally, the influence of axial compression ratio and longitudinal reinforcement on energy dissipation capacity and stiffness degradation were studied based on the validated finite element modes. It is confirmed that tubular reinforced concrete columns of air-cooling condenser structure exhibit a moderate ability of energy dissipation, and the nonlinear finite element model could reasonably simulate its seismic behavior. Furthermore, axial compression ratio and longitudinal reinforcement are main factors which affect the seismic behavior of the tubular reinforced concrete columns. The experimental results and simulation method provide an available way to design this kind of large tubular reinforced concrete columns with thin-wall.
Explore Rural Female Elders’ Old-Age-Provision Plight and Solutions
Fangqin Du,Jun Liang,Yan Huang 숙명여자대학교 아시아여성연구원 2009 Asian Women Vol.25 No.4
By the Participatory-Need-Assessing and in-depth interviews we began to investigate the current living situation of the rural female elders at five sampled villages scattered in three of the counties or cities in Henan - a province located in Central China with a large rural population. We found a very strange and contradictory phenomenon: “Strong Son- preference” for “Raising-Son-for-Old-Age-Nurturing” VS the “Having-Son-But-Having-No-Old-Age-Provision” Plight. After many participatory discussions with the villagers, we realize that the roots lie in the patriarchic principles, which still directing the rural families. The first is the “patrilineal family succession”, or to carry on the line of the family by following the father-to-son principle; the second is the “son staying in, daughter moving out” marital residency convention, which constraints a married woman being the member of her husband’ familiy but not her native family; the third is the sex-based labor division principle of “men running without, woman within.” These lend to two vicious cycles: “Raising-Son-for-Old-Age-Nurturing” but the “Having-Son-But-Having-No-Old-Age-Provision”, the conflicts for centuries between the daughter-in-law and the mother-in-law. As one feminist action research, we are exploring to break through the patriarchal institution and promote a diversified old-age-provision model, including 1) the family-based old-ageprovision such as the son and daughter respectively or united providing their parents, the parents providing themselves; 2) the community-based old-ageprovision; 3) the government-based old-age-provision.
( Ming-liang Kong ),( Chun-lan Du ),( Hua-song Mao ),( Jun-hua Zhang ),( Kwang-min Ham ),( Jo Hyunju ) 한국환경과학회 2016 한국환경과학회지 Vol.25 No.7
This study focused on the buildings, wharves, and parks of the coastal space of Chongqing based on the ten-year construction draft plan of the alternate capital. From an investigation of these three components according to their type, structure, terrain, function, spatial layer, and so on, the following conclusions were drawn: The coastal space of Chongqing is composed of three different layers. The first and second layers consisted of the two-river intersection basin with the transportation and commemorative space at the center, the Yangtze River basin with the commercial space at the center, and the Jialing River with the military and recreational space at the center. The third-layer is centered around the commemorative space. The coastal space of Chongqing manifested the limitations of the times, its traditionality, and an integrated development process.
Flexible cascaded multilevel inverter with multiple operation modes
Wang, Yaoqiang,Du, Guanyu,Liang, Jun,Qin, Ming The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.3
In this paper, a flexible cascaded multilevel inverter is proposed with a wide operational range. The inverter can change its topology structure to operate in three modes by a bidirectional switch unit. The nine-level or five-level mode of the inverter is adopted to optimize the output waveform when the input power is low. To decrease power losses, the three-level mode of the inverter is adopted to reduce the number of active switch devices when the input power is high. The topology and modulation strategy of the proposed inverter are presented and analyzed. The total losses and current THD of the inverter are calculated. In addition, simulations and experiments are conducted. The obtained simulation and experimental results indicate the correctness and feasibility of the proposed inverter and its modulation strategy.
Zhen-Xian Du,Hai-Yan Zhang,Da-Xin Gao,Hua-Qin Wang,Yong-Jun Li,Guo-Liang Liu 생화학분자생물학회 2006 Experimental and molecular medicine Vol.38 No.3
apoptosis protein (IAP) family, which is known to be over-expressed in various carcinomas and associated with their biologically aggressive characteristics. The aim of this study was to investigate survivin expression in human medullary thyroid carcinoma (MTC) and a MTC cell line TT, correlate suvivin expression with clinicopathologic features of MTC, and test effects of antisurvivin oligonucleotides (ASODNs) on growth and apoptosis of TT cells. Survivin expression was immunohistochemically determined in formalin-fixed and paraffinembedded specimens obtained from 10 cases of normal thyroid (NT) and 10 cases of MTC, and in TT cells. In TT cells, we confirmed survivin expression and its down-regulation by ASODNs using RT-PCR and Western blot analyses, and investigated effects of ASODNs on viability and growth by MTT assay and apoptosis by apoptotic analyses including DNA laddering assay, acridine orange/ethidium bromide staining and flow cytometric cell cycle analysis. Immunohistochemical analysis showed high survivin expression in MTC and TT cells, whereas no immunoreactivity was detectable in NT. Statistical analyses revealed no significant correlation of survivin expression with the clinicopathologic features of MTC. In TT cells, survivin expression at both mRNA and protein levels was confirmed and could be down-regulated by ASODNs concomitant with decrease in viability and growth, and increase in apoptosis. Our results suggest that survivin plays an important role in MTC independent of the conventional clinicopathologic factors, and ASODNs is a promising survivin- targeted gene therapy for MTC.
The development of training platform for CiADS using cave automatic virtual environment
Jin-Yang Li,Jun-Liang Du,Long Gu,You-Peng Zhang,Xin Sheng,Cong Lin,Yongquan Wang Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.7
The project of China initiative Accelerator Driven Subcritical (CiADS) system has been started to construct in southeast China's Guangdong province since 2019, which is expected to be checked and accepted in the year 2025. In order to make the students in University of Chinese Academy of Sciences (UCAS) better understand the main characteristic and the operation condition in the subcritical nuclear facility, the training platform for CiADS has been developed based on the Cave Automatic Virtual Environment (CAVE) in the Institute of Modern Physics Chinese Academy of Sciences (IMPCAS). The CAVE platform is a kind of non-head mounted virtual reality display system, which can provide the immersive experience and the alternative training platform to substitute the dangerous operation experiments with strong radioactivity. In this paper, the CAVE platform for the training scenarios in CiADS system has been presented with real-time simulation feature, where the required devices to generate the auditory and visual senses with the interactive mode have been detailed. Moreover, the three dimensional modeling database has been created for the different operation conditions, which can bring more freedom for the teachers to generate the appropriate training courses for the students. All the user-friendly features will offer a deep realistic impression to the students for the purpose of getting the required knowledge and experience without the large costs in the traditional experimental nuclear reactor.
Highly efficient removal of three red dyes by adsorption onto Mg–Al-layered double hydroxide
Ran-ran Shan,Liang-guo Yan,Yan-ming Yang,Kun Yang,Shu-jun Yu,Hai-qin Yu,Bao-cun Zhu,Bin Du 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1
The Mg–Al-CO3-LDH with Mg2+/Al3+ molar ratio of 2 was prepared via coprecipitation method atconstant pH of 9–10 and used to remove three red dyes by batch adsorption method. The results showedthe Mg–Al-LDH were well crystallized and can adsorb the red dyes effectively. The optimal adsorbentdosage and contact time were 1.0 g and 60 min, respectively. Adsorbed amount hardly changed whenpH < 10. The adsorption kinetics fit the pseudo-second order kinetic models well and isothermscorrespond to Langmuir model strictly. All the relevant mechanisms were studied and manifested asanion exchange and also electrostatic attraction for CR.