Docetaxel-loaded RIPL peptide (IPLVVPLRRRRRRRRC)-conjugated liposomes: Drug release, cytotoxicity, and antitumor efficacy

Yoon, Ho Yub,Kwak, Seong Shin,Jang, Moon Ho,Kang, Min Hyung,Sung, Si Woo,Kim, Chang Hyun,Kim, Sung Rae,Yeom, Dong Woo,Kang, Myung Joo,Choi, Young Wook Elsevier 2017 International journal of pharmaceutics Vol.523 No.1

<P><B>Abstract</B></P> <P>We previously synthesized the RIPL peptide (IPLVVPLRRRRRRRRC) to facilitate selective delivery into hepsin-expressing cancer cells and showed that RIPL peptide-conjugated liposomes (RIPL-L) enhanced the intracellular delivery of fluorescent probes <I>in vitro</I>. In this study, docetaxel-loaded RIPL-L (DTX-RIPL-L) were prepared and evaluated for <I>in vitro</I> drug release, cytotoxicity, and <I>in vivo</I> antitumor efficacy. DTX was successfully encapsulated by pre-loading, with an average encapsulation efficiency and drug loading capacity of 32.4% and 21.39±2.05 (μg/mg), respectively. A DTX release study using dialysis showed a biphasic release pattern, i.e., rapid release for 6h, followed by sustained release up to 72h. The first-order equation provided the best fit for drug release (r<SUP>2</SUP> =0.9349). <I>In vitro</I> cytotoxicity was dose-dependent, resulting in IC<SUB>50</SUB> values of 36.10 (SK-OV-3) and 48.62ng/mL (MCF-7) for hepsin-positive, and 61.12 (DU145) and 53.04ng/mL (PC-3) for hepsin-negative cell lines. Live/dead cell imaging was carried out to visualize the proportion of viable and nonviable SK-OV-3 cells. Compared to DTX solution, DTX-RIPL-L significantly inhibited tumor growth and prolonged survival time in BALB/c nude mice with SK-OV-3 cell tumors. We suggest that DTX-RIPL-L is a good candidate for efficient drug targeting to hepsin-expressing cancer cells.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Implementation of Timing Synchronization in Vehicle Communication System

Lee, Sang-Yub,Lee, Chul-Dong,Kwak, Jae-Min The Korea Institute of Information and Commucation 2010 Journal of information and communication convergen Vol.8 No.3

In the vehicle communication system, transferred information is needed to be detected as possible as fast in order to inform car status located in front and rear side. Through the moving vehicle information, we can avoid the crash caused by sudden break of front one or acquire to real time traffic data to check the detour road. To be connecting the wireless communication between the vehicles, fast timing synchronization can be a key factor. Finding out the sync point fast is able to have more marginal time to compensate the distorted signals caused by channel variance. Thus, we introduce the combination method which helps find out the start of frame quickly. It is executed by auto-correlation and cross-correlation simultaneously using only short preambles. With taking the absolute value at the implemented synch block output, the proposed method shows much better system performance to us.

군날개 제거술 : 광범위 절제술 및 자가윤부결막이식술

곽동엽,배민철,이진기,박대진,Dong Yub Kwak,Min Chul Bae,Jin Kee Lee,Dae Jin Park 대한안과학회 2008 대한안과학회지 Vol.49 No.2

`Purpose: To evaluate the efficacy of conjunctivo-limbal autograft after wide excision of primary and recurrent pterygia. Methods: Twenty-one eyes of 18 patients with primary pterygium and 18 eyes of 18 patients with recurrent pteygium underwent conjunctivo-limbal autograft after wide excision of pterygium. All patients underwent follow-up for more than six months. Recurrence rates and complications were evaluated. Results: With a minimum of six months of follow-up, fibrovascular tissue in the excised area, not invading the cornea, was noted in one eye (5.6%) in the recurrent pterygium group but no further surgical interventions for the cosmetic problem were needed. One eye (4.8%) showed wound dehiscence, three eyes (14.3%) showed subgraft hemorrhage, and one eye (4.8%) showed subconjunctival fibrosis at the donor site in the primary pterygium group, while two eyes (11.1%) showed subgraft hemorrhage, and one eye (5.6%) showed Tenon's Capsule granuloma at the donor site in the recurrent pterygium group. Conclusions: Conjunctivo-limbal autograft after wide excision of pterygium can be considered an effective treatment with low recurrence rates for both primary and recurrent pterygia. `

군날개 제거술 : 광범위 절제술 및 조직접합제를 이용한 양막이식

곽동엽,이진기,박대진,Dong Yub Kwak,Jin Kee Lee,Dae Jin Park 대한안과학회 2008 대한안과학회지 Vol.49 No.2

`Purpose: To compare the efficacy and safety of fibrin glue and sutures for amniotic membrane transplantation after wide excision of primary pterygium. Methods: Forty-three eyes of 37 patients underwent amniotic membrane transplantation for primary pterygium with a minimum follow-up period of six months. Twenty-three eyes of 18 patients underwent surgery with fibrin glue and 20 eyes of 19 patients underwent surgery with sutures. Recurrence rates, complications, operating time were evaluated. Results: With a minimum of six-month of follow-up, fibrovascular tissue in the excised area, not invading the cornea (conjunctival recurrence), was noted in four eyes (17.4%) and fibrovascular tissue invading the cornea (corneal recurrence) was noted in two eyes (8.7%) in the fibrin glue group. Conjunctival recurrence was noted in five eyes (25%) and corneal recurrence was noted in one eye (5%) in the suture group. There was no significant difference in the recurrence rates between the two groups. Mean operating time in the fibrin glue group (25.2±3.5 minutes) was significantly shorter than in the suture group (40.5±3.6 minutes) (p=0.001, Students t-test). Complications included sub-amniotic membrane hemorrhage in three eyes (13%), and granuloma in one eye (4.3%) in the fibrin glue group, sub-amniotic membrane hemorrhage in four eyes (20%), granuloma in three eyes (15%), and wound dehiscence in one eye (5%) in the suture group. Conclusions: Using fibrin glue instead of sutures in amniotic membrane transplantation after wide excision of pterygium can be considered an effective treatment with shorter operating time and fewer complications, although there is no significant difference in recurrence rates.`

한 눈 중등도 일차하사근기능항진 환자에서 하사근후전술 후 발생한 반대편 눈의 하사근기능항진

문성혁,곽동엽,김명미.Sung Hyuk Moon. MD. Dong Yub Kwak. MD. Myung Mi Kim. MD. PhD 대한안과학회 2013 대한안과학회지 Vol.54 No.12

Purpose: To investigate the incidence rate of inferior oblique muscle overaction (IOOA) in the contralateral eye and the effect of inferior oblique (IO) muscle recession of the contralateral eye in the patients who received IO muscle recession for unilateral moderate (+2 or +3) primary IOOA. <br /> Methods: Medical records of 88 patients with unilateral primary IOOA who underwent unilateral IO muscle recession were retrospectively reviewed and observed during a follow-up period of more than 1 year. Graded recession of the IO muscle was performed according to the degree of IOOA. If postoperative IOOA was below +1, the surgery was considered successful. If IOOA in the contralateral eye was more than +2 after surgery, the IOOA was considered to have occurred.<br /> Results: In cases where IOOA was +2 and +3 before the surgery, the success rate was 98.2% and 100%, respectively, showing an overall success rate of 98.8%. The incidence of contralateral IOOA after recession of the unilateral IO muscle was 24.1% in the +2 group and 16.6% in the +3 group with an overall incidence rate of 21.6% and when the IO muscle recession of the contralateral eye was performed, the success rate was 100%. <br /> Conclusions: The incidence of contralateral IOOA was 21.6% after the unilateral IO muscle recession in moderate unilateral primary IOOA. A satisfactory outcome was obtained through an additional IO muscle recession in the contralateral eye. <br /> J Korean Ophthalmol Soc 2013;54(12):1888-1892

학교수준의 부가가치모델을 통한 학교 효율성 척도 연구

홍사훈 ( Saahoon Hong ),류지훈 ( Ji Hoon Ryoo ),곽노훈 ( Nohoon Kwak ),이동엽 ( Dong Yub Lee ) 경북대학교 중등교육연구소 2017 중등교육연구 Vol.65 No.3

본 연구에서는 한국교육종단연구 1-3차년도 데이터를 사용하여 중학생들의 학업성취도에 영향을 미치는 학교단위 변인들을 탐색하였다. 학교단위의 변인들을 바탕으로 네 가지의 학교단위 부가가치모델(value-added model)을 구현함으로써 학교 효율성 척도를 산출하였다. 특히 전체학생들과 학업성취도 수준이 낮은 학생들에 대한 학교의 효율성(effectiveness)을 구분함으로써 모집단이 달라짐에 따른 학교 효율성 차이를 확인하고자 하였다. 연구 결과, 제시된 네 가지 부가가치모델은 통계적으로 유의미한 차이를 보였다. 같은 과목 내에서의 모델 간 학교 효율성 척도 상관관계는 대부분 0.8 이상으로 높았지만, 각 모델 내에서 영어와 수학에 대한 학교 효율성 척도 상관관계는 0.39부터 0.44로 낮았다. 이는 부가가치모델이 학교 간의 차이를 나타내는 다양한 변인들을 포함할 수 있음을 보여준다. 또한 과목 간의 낮은 상관관계를 통해 알 수 있듯이 부가가치모델을 통한 효율성 척도는 각 과목별로 해석되고 활용되어져야 함을 의미한다. As student learning gains depend on the quality of school, effective school must be a primary measure of its quality. However, the evaluation of school is at best inconsistent, which is mainly because of the lack of reliable and valid evaluation tools. This study examines four value-added models (VAMs) to measure school accountability by using panel data from the first, second, and third waves of the Korean Education Longitudinal Study. VAM estimates yields the academic growth rates of groups of all students in middle school and low performing students, respectively, from year to year. The results indicate that VAM estimates from four different VAMs are significantly different from one another. In particular, correlations between Math and Reading in each VAM vary between 0.39 and 0.44, while correlations among VAM estimates within each subject are above 0.8. The findings suggest that critical school variables could be taken differently to compare the school effectiveness in Math and Reading, respectively. VAM estimates also need to be interpreted by subjects, Math and Reading, separately. Recommendations for policy associated with VAMs and future research topics are discussed.

PDP 투명전극의 응용을 위한 ZnO:Al 박막의 제작 및 평가

박강일(Kang-il Park),김병섭(Byung-Sub Kim),김현수,임동건(Dong-Gun Lim),박기엽(Gi-Yub Park),이세종(Se-Jong Lee),곽동주(Dong-Joo Kwak) 대한전기학회 2003 대한전기학회 학술대회 논문집 Vol.2003 No.7