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Dissanayaka, Dissanayaka M.S.,De Silva, Sembukuttige N.T.,Attanayaka, D.P.S.T.G.,Kurera, Mihidukulasuriya J.M.S.,Fernando, Charakrawarthige A.N. The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.3
The present study reports the morphological and molecular characterization of the fungal strain, CMSS06 and evaluates its raw starch hydrolyzing ability in four different agricultural substrates (rice bran, banana peel, cassava tubers, and coconut water). The potential use of each agricultural substrate to replace the expensive fermentation media was evaluated with six different fermentation media: rice bran (RB), banana peel (BP), cassava starch (CS), cassava in coconut water (CSCW), cassava in modified coconut water (CMCW), and pure Coconut water (CW). The fungal strain CMSS06 was identified as Thielaviopsis ethacetica by the analysis of the ITS sequences. The T. ethacetica alpha amylase enzyme exhibited maximum alpha amylase activity at 72 h, pH 7.0, and $40^{\circ}C$ on soluble starch. This species resulted in the highest enzyme activity (mU/ml) of 26.06, 10.89, 58.82, 14.2, and 54.67 with the RB, BP, CS, CSCW, and CMCW fermentation media, respectively. The results indicate that CS can be used as a carbon substrate and CMCW can be used to accelerate the fermentation by T. ethacetica. The enzyme was partially purified by 40-60% ammonium sulphate fraction, and it showed total enzyme activity, total protein content, specific activity, purification fold, and a recovery of 2400 mU, 30 mg, 80 mU/mg, 2.7, and 71.1%, respectively. The molecular mass of the T. ethacetica alpha amylase was estimated on SDS-PAGE, and two bands around 50 kDa and 70 kDa were identified. The present study implies that T. ethacetica can produce alpha amylase, and it can be used to hydrolyze raw starch during the fermentation processes.
( Dissanayaka M. S. Dissanayaka ),( Sembukuttige N. T. De Silva ),( D. P. S. T. G. Attanayaka ),( Mihidukulasuriya J. M. S. Kurera ),( Charakrawarthige A. N. Fernando ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 한국미생물·생명공학회지 Vol.47 No.3
The present study reports the morphological and molecular characterization of the fungal strain, CMSS06 and evaluates its raw starch hydrolyzing ability in four different agricultural substrates (rice bran, banana peel, cassava tubers, and coconut water). The potential use of each agricultural substrate to replace the expensive fermentation media was evaluated with six different fermentation media: rice bran (RB), banana peel (BP), cassava starch (CS), cassava in coconut water (CSCW), cassava in modified coconut water (CMCW), and pure Coconut water (CW). The fungal strain CMSS06 was identified as Thielaviopsis ethacetica by the analysis of the ITS sequences. The T. ethacetica alpha amylase enzyme exhibited maximum alpha amylase activity at 72 h, pH 7.0, and 40℃ on soluble starch. This species resulted in the highest enzyme activity (mU/ml) of 26.06, 10.89, 58.82, 14.2, and 54.67 with the RB, BP, CS, CSCW, and CMCW fermentation media, respectively. The results indicate that CS can be used as a carbon substrate and CMCW can be used to accelerate the fermentation by T. ethacetica. The enzyme was partially purified by 40-60% ammonium sulphate fraction, and it showed total enzyme activity, total protein content, specific activity, purification fold, and a recovery of 2400 mU, 30 mg, 80 mU/mg, 2.7, and 71.1%, respectively. The molecular mass of the T. ethacetica alpha amylase was estimated on SDS-PAGE, and two bands around 50 kDa and 70 kDa were identified. The present study implies that T. ethacetica can produce alpha amylase, and it can be used to hydrolyze raw starch during the fermentation processes.
Charuka Moremada,Chamara Sandeepa,Nadeeka Dissanayaka,Tharindu Gamage,Madhusanka Liyanage 한국통신학회 2021 Journal of communications and networks Vol.23 No.5
Due to the spread of Coronavirus disease 2019(COVID-19), the world has encountered an ongoing pandemicto date. It is a highly contagious disease. In addition to thevaccination, social distancing and isolation of patients are provento be one of the commonly used strategies to reduce the spread ofdisease. For efficient social distancing, contact tracing is a criticalrequirement in the incubation period of 14-days of the disease tocontain any further spread. However, we identify that there is alack of reliable and practical social interaction tracking methodsand prediction methods for the probability of getting the disease. This paper focuses on user tracking and predicting the infectionprobability based on these social interactions. We first developedan energy-efficient BLE (Bluetooth Low Energy) based socialinteraction tracking system to achieve this. Then, based on thecollected data, we propose an algorithm to predict the possibilityof getting the COVID-19. Finally, to show the practicality of oursolution, we implemented a prototype with a mobile app anda web monitoring tool for healthcare authorities. In additionto that, to analyze the proposed algorithm’s behaviour, weperformed a simulation of the system using a graph-based model.
( Ambrish Singh ),( Anirudh Kotlo ),( Zhiqiang Wang ),( Thusharika Dissanayaka ),( Siddharth Das ),( Benny Antony ) 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.1
Background/Aims: Conventional disease-modifying anti-rheumatic drugs have been trialed in osteoarthritis (OA). Hydroxychloroquine (HCQ), which has shown its effectiveness in rheumatoid arthritis, has been trialed for the treatment of OA; however, its efficacy and safety remain unclear. This systematic review and meta-analysis evaluate efficacy and safety of HCQ for the treatment of OA. Methods: MEDLINE, EMBASE, and Cochrane Central were searched from inception through June 2020. Two reviewers independently screened for randomized controlled trials (RCTs) comparing HCQ with placebo or other active-comparators for the treatment of knee, hand, or hip OA, extracted data, and performed Cochrane risk of bias assessments. Results: Six RCTs, four in hand OA, two in knee OA, consisting of 842 patients (436 in HCQ arm, 406 in control arm) were included. RCTs were conducted between 2012 and 2020, one each at UK, Netherlands, Germany, Italy, Iran, and Egypt; follow-up period ranged 24 to 52 weeks. High-quality evidence showed no clinically important pain reduction with HCQ compared to placebo/active-control in hand OA (standardized mean difference [SMD], 0.14; 95% confidence interval [CI], -0.20 to 0.48). Effect on pain reduction in knee and hand OA was small and non-significant (SMD, -0.09; 95% CI, -0.44 to 0.25). High-quality evidence showed no improvement in dysfunction with HCQ compared to placebo in hand OA patients (SMD, 0.08; 95% CI, -0.23 to 0.40). Effect on dysfunction improvement in knee and hand OA was modest and statistically non-significant (SMD, -0.20; 95% CI, -0.57 to 0.18). No improvement in quality of life was observed in hand OA. Conclusions: HCQ has no benefit in reducing pain and improving physical function in hand or knee OA patients.