Production of Transgenic Stem Mustard Plants Co-expressing Coat Protein Genes of TUMV and CMV

De-Ping Guo,Wei Zhang,Ji-Shuang Chen,Kai Zhang,Qiao-Mei Wang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Efficacy and Safety of Intravenous Urapidil for Older Hypertensive Patients with Acute Heart Failure: A Multicenter Randomized Controlled Trial

Wei Yang,Qi Hua,Yu-Jie Zhou,Yan Fu,Jian Qin,Shu Qin,Xiao-Min Chen,Jin-Cheng Guo,De-Zhao Wang,Hong Zhan,Jing Li,Jing-Yu He 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.1

Purpose: Urapidil is putatively effective for patients with hypertension and acute heart failure, although randomized controlled trials thereon are lacking. We investigated the efficacy and safety of intravenous urapidil relative to that of nitroglycerin in older patients with hypertension and heart failure in a randomized controlled trial. Materials and Methods: Patients (>60 y) with hypertension and heart failure were randomly assigned to receive intravenous urapidil (n=89) or nitroglycerin (n=91) for 7 days. Hemodynamic parameters, cardiac function, and safety outcomes were compared. Results: Patients in the urapidil group had significantly lower mean systolic blood pressure (110.1±6.5 mm Hg) than those given nitroglycerin (126.4±8.1 mm Hg, p=0.022), without changes in heart rate. Urapidil was associated with improved cardiac function as reflected by lower N terminal-pro B type natriuretic peptide after 7 days (3311.4±546.1 ng/mL vs. 4879.1±325.7 ng/mL, p=0.027) and improved left ventricular ejection fraction (62.2±3.4% vs. 51.0±2.4%, p=0.032). Patients given urapidil had fewer associatedadverse events, specifically headache (p=0.025) and tachycardia (p=0.004). The one-month rehospitalization and all-cause mortality rates were similar. Conclusion: Intravenous administration of urapidil, compared with nitroglycerin, was associated with better control of blood pressure and preserved cardiac function, as well as fewer adverse events, for elderly patients with hypertension and acute heart failure.

Inhibition of cancer cell growth and migration by dihydroxynaphthyl aryl ketones

Wei Xiong,Yun-Feng Li,Shan Liu,Ting Chen,Hong-Tao Zhang,Zhi-Bin Yang,Ying-Ying Ding,De-Pei Gao,Guan-Shun Wang,Jian Dong,Jian Dong 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.4

Dihydroxynaphthyl aryl ketones 1-5 exhibit activity as tubulin polymerization inhibitors by targeting the colchicine binding site of microtubules making them potential anticancer drugs. Therefore, analogues 1-5 have been evaluated for their cytotoxic activity against the cancer cell lines DU-145 (prostate), T24 (bladder) and MCF-7 (breast). notable differences in biological activity were observed for compounds 1-5, most likely related to the nature of the aryl substituent bonded to the carbonyl group. among the tested compounds, only compound 5 showed selectivity for cancer cells over healthy, non-transformed cells. T24 cancer cells treated with compound 5 presented a concentration-dependent decrease in cell proliferation and a loss of migration ability. The cytotoxicity of compounds 1-5 on the selected cell-based assays is discussed in terms of it lipophilicity and polarizability parameters.

Characteristics of Carbohydrate Assimilation and Distribution in Walnut (Juglans regia L.)

Shi-Wei Wang,Cun-De Pan,Cui-Fang Zhang,Hong Chen 한국원예학회 2021 원예과학기술지 Vol.39 No.2

Based on <SUP>13</SUP>C isotope tracer technology, we investigated the <SUP>13</SUP>C abundance (δ<SUP>13</SUP>C), <SUP>13</SUP>C content(<SUP>13</SUP>Camount), and new fixed <SUP>13</SUP>C percentage (<SUP>13</SUP>CPCT) in different organs and different metabolic components in girdled fruit-bearing shoots at different stages of fruit growth and development in walnut. Our aim was to analyze the dynamic characteristics of carbohydrate assimilation, transportation, and utilization in different stages of walnut fruit growth and development. The results showed that (1) at 0 h after the feeding, δ<SUP>13</SUP>C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT in sucrose from the leaves were the highest and positively correlated with net photosynthetic rate of leaves (p < 0.05); (2) at 24 h after the feeding, δ13C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT in sucrose from the carpopodium reached their peak values; (3) δ<SUP>13</SUP>C,<SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT in sucrose from the peel were lower than those of hexose at 48 h after feeding in the fast-growing stage, but they all reached maximum levels in peel sucrose at 48 h after feeding in the oil conversion stage; and (4) the highest δ<SUP>13</SUP>C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT were recorded in all carbohydrates from the seed kernel 48 h after feeding, among which the highest δ<SUP>13</SUP>C, <SUP>13</SUP>Camount, and <SUP>13</SUP>CPCT were recorded in sucrose. These results suggested that sucrose was the main form of carbohydrate assimilation and transportation in walnut fruit, and large amounts of sucrose are accumulated in the peel and in the kernel, where it is also the base for the transformation of oil.

Integrin-linked Kinase Functions as a Tumor Promoter in Bladder Transitional Cell Carcinoma

Wang, De-Lin,Lan, Jian-Hua,Chen, Liang,Huang, Biao,Li, Zeng,Zhao, Xiu-Min,Ma, Qiang,Sheng, Xia,Li, Wen-Bin,Tang, Wei-Xue Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

The aim of this study was to elucidate the role of the integrin-linked kinase (ILK) gene in development of human bladder transitional cell carcinoma (BTCC). Expression of ILK protein and ILK mRNA in 56 cases of human BTCC tissue and in 30 cases of adjacent normal bladder tissue was detected by immunohistochemistry S-P and reverse transcription polymerase chain reaction (RT-PCR), respectively. Four specific miRNA RNAi vectors targeting human ILK were synthesized and transfected into BIU-87 cells by liposome to obtain stable expression cell strains. The influence of ILK on proliferation of BTCC was detected by MTT, FCM on athymic mouse tumorigenesis. The positive rate of ILK protein in BTCC tissue (53.6%) was much higher than adjacent normal bladder tissue (10.0%) (p<0.05). Similarly, expression of ILK mRNA in BTCC tissue ($0.540{\pm}0.083$) was significantly higher than in adjacent normal bladder tissue ($0.492{\pm}0.070$) (p<0.05). MTT showed that the proliferation ability of miRNA-ILK transfected group was clearly decreased (p<0.05), the cell cycle being arrested in G0/G1-S, an tumorigenesis in vivo was also significantly reduced (p<0.05). ILK gene transcription and protein expression may be involved in the development of BTCC, so that ILK might be the new marker for early diagnosis and the new target for gene treatment.

Arm Exoskeleton Rehabilitation Robot with Assistive System for Patient after Stroke

Guan De Lee,Wei-Wen Wang,Kai-Wen Lee,Sheng-Yen Lin,Li-Chen Fu,Jin-Shin Lai,Wen-Shiang Chen,Jer-Junn Luh 제어로봇시스템학회 2012 제어로봇시스템학회 국제학술대회 논문집 Vol.2012 No.10

Exercise dosage is proven to be an important factor in the physical treatment. Robot assistive approach can improve the rehabilitation quality and evaluate patient’s recovery quantitatively. This paper presents kinematic structure, assistive control system, and integrated F/T sensor for an upper limb rehabilitation robot. By using the human arm dynamic, there are three rehabilitation modes presented in this paper: active mode, assistive mode, and passive mode. In assistive mode, we have two strategies to implement it. One is to amplify interactive torque, and the other is to apply assist-as-needed concept. The goal of this mode is to assist patients to finish motion tasks. The rehabilitation robot under investigation has 7 degree of freedom (DOF) actuated by DC motors, which are programmed to drive the robot arm in the 3D space. To validate our control design, some realistic experiments are conducted and its satisfactory performance is demonstrated. This work is approved clinical testing by the Department of Health, Executive Yuan, R.O.C. So far, we are demonstrating the effect of our controller.

Prevalence of Spina Bifida Occulta and Its Relationship With Overactive Bladder in Middle-Aged and Elderly Chinese People

Jun Wei Wu,Yu Rong Xing,Yi Bo Wen,Tian Fang Li,Quan De Feng,Xiao Ping Shang,Yun Long Li,Jin Jin Feng,Xin Xin Wang,Rong Qun Zhai,Xiang Fei He,Tao Chen,Xin Jian Liu,Jian Guo Wen 대한배뇨장애요실금학회 2016 International Neurourology Journal Vol.20 No.2

Purpose: To investigate the prevalence of spina bifida occulta (SBO) and its relationship with the presence of overactive bladder (OAB) in middle-aged and elderly people in China. Methods: A cross-sectional community-based survey was carried out at 7 communities in Zhengzhou City, China from December 15, 2013 to June 10, 2014, where residents aged over 40 years were randomly selected to participate. All of the participants underwent lumbosacral radiographic analysis and relevant laboratory tests. A questionnaire including basic information, past medical history and present illness, and the OAB symptom score was filled out by all participants. Chi-square tests and logistic regression were used for data analysis with a P-value of <0.05 denoting statistical significance. Results: A total of 1,061 subjects were qualified for the final statistical analysis (58.8±11.7 years; male, 471 [44.4%]; female, 590 [55.6%]). The overall prevalence of SBO was 15.1% (160 of 1,061): 18.3% (86 of 471) in men and 12.5% (74 of 590) in women. Among these subjects, 13.7% (145 of 1,061) had OAB: 13.2% (62 of 471) in men and 14.1% (83 of 590) in women. The results of logistic regression showed that age, SBO, history of cerebral infarction (HCI), and constipation were risk factors for OAB (P<0.05), while sex, history of childhood enuresis (HCE), body mass index (BMI), and diabetes mellitus (DM) were not (P>0.05). In men, age, SBO, and constipation were risk factors for OAB (P<0.05), while HCE, BMI, DM, HCI, and benign prostate hyperplasia were not (P>0.05). In women, age, SBO, and HCI were risk factors for OAB (P<0.05), while HCE, BMI, DM, vaginal delivery, and constipation were not (P>0.05). Conclusions: The prevalence of SBO is high and it is related to OAB in middle-aged and elderly people in China.

Investigation of the Photoinduced Anisotropy of the Bacteriorhodopsin/Polymer Composite Film by Using Probing-Pumping Beams of Various Wavelengths

Liang-Yao Chen,Su-Xing Pan,Yun-Fei Wu,Lai Wei,Yu-Xiang Zheng,You-Yuan Zhao,De-Wang Ma 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.4

We have investigated the probing beam transmitance due to the photon-induced anisotropy effect in the bacteriorhodopsin/polymer composite film(bR/PC film) by using the probing-pumping beam method under different laser wavelengths. By using a theoretical simulation, we give the characteristics of the birefringence Δn and the dichroism ΔD for various wavelengths of the probing and the pumping beams.

Research on Three-Component Geomagnetic Field Differential Measurement Method for Underwater Vehicle

Zhao Ta,Chen Yu Wei,Zhou Zhi Jian,Cheng De Fu 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.2

The precise measurement of the geomagnetic element is the key to realize navigation and positioning with the development of geomagnetic navigation technology. In this paper, a kind of underwater vehicle three-component geomagnetic field differential measurement method is presented based on the ideas of the difference. The threecomponent geomagnetic field mathematical model of traditional measurement method is improved and new differential measurement model(DMM) is established. Difference expressions of measurement magnetic field in the DMM obviously reduce the impact of interference magnetic field in the process of geomagnetic field measurement and improve the measuring precision of the three-component geomagnetic field. Finally, the method’s effectiveness is validated by simulation. The precision of DMM method is 10 ± 5nT which is about two times that of traditional measurement model (TMM) method under the condition of ± 25nT stray outside interference magnetic field. The method effectively improves the geomagnetic field measurement precision and has stronger antiinterference ability. It is of significance for practical application of underwater geomagnetic navigation method.

Enhanced Performance of Pseudo-Bilayer Organic Photovoltaic Devices via Small Molecule Doping

Syu, Yu-Wei,Huang, Peng-Yi,Li, Husan-De,Hsu, Ching-Ling,Chiu, Kuan-Cheng,Kim, Choongik,Chen, Ming-Chou,Chao, Yu-Chiang American Chemical Society 2014 The Journal of Physical Chemistry Part C Vol.118 No.19

<P>Controlling both the film crystallinity of the active layer for better charge transport and the interdiffusion between donor and acceptor materials for optimal bicontinuous networks is essential in producing pseudo-bilayer polymer solar cells. In this work, we investigated the influence of a doping solution-processable small molecule with high carrier mobility, 5,11-bis(triethylsilylethynyl) anthradithiophene (TES-ADT), on the performance of pseudo-bilayer polymer solar cells made of an underlayer of poly(3-hexylthiophene) (P3HT) and an upper layer of [6,6]-phenyl-C61-butyric acid methyl ester (PCBM). By analysis of the X-ray diffraction and UV–vis absorbance spectra of P3HT:TES-ADT blend films it was demonstrated that the film crystallinity was enhanced by TES-ADT doping in the P3HT underlayer. The hole mobility extracted from the current density–voltage curves of hole-only devices based on P3HT:TES-ADT demonstrated an optimized value with proper TES-ADT doping and thermal annealing. An intermixed photoactive layer was observed for the annealed device, indicating the occurrence of interdiffusion with a large interfacial area. With improved film crystallinity and interdiffusion, the optimal device performance was obtained when 5% TES-ADT was blended with P3HT and a thermal annealing treatment at 150 °C for 1 min was conducted. At that optimal condition, the mean crystallite size was increased by 35%, and hence the enhancement of 8% and 14% in power conversion efficiency and short-circuit current density was observed, respectively.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2014/jpccck.2014.118.issue-19/jp502331x/production/images/medium/jp-2014-02331x_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jp502331x'>ACS Electronic Supporting Info</A></P>