螢光抗體間接法과 鷄腦脊髓炎 바이러스의 抗原性 및 再現性에 관한 硏究

崔源弼 慶北大學校 1976 論文集 Vol.21 No.-

An indirect fluorescent antibody technique (IDFAT) for the detection of avian encephalomyelitis (AE) antibody was recently reported by Choi and Miura^(6). They indicated that the test was more sensitive and specific than virus-neutralization test. This note deals with the preparation of antigen, antigenicity of stock-antigen and the reliability of an IDFAT to examine AE antibody in chickens. The results obtained are summerized as follows: 1. The antigen of AE for IDFAT was produced by the susceptible eggs which were inoculated (yolk-sac-method) with 10^3.0 EID_50/0.1ml and 10^5.5 EID_50/0.1ml of AEV-VR strain after 6 days of incubation, and the cerebrum was removed 8-12 days and 6-10 days later to make slide smears. 2. By inoculation with 10^5.0 EID_50/0.1ml of AEV-VR strain, the AE antigen for IDFAT could be prepared from embryos which are produced by so-called AE resistant chicken flocks, as well as embryos from susceptible flocks. 3. The fixed antigens could be stored at 4℃ for 40 days and -20℃ for 6 months or longer without change of the antigenicity. 4. Reappearene of the IDFAT for the detection of AE-virus antibody showed a high reliability.

디지털카메라를 이용한 3차원 측량 기법 개발에 관한 연구

崔哲淳,崔勝弼,韓元浩,金東熙 관동대학교 1997 關大論文集 Vol.25 No.2

Photographic survey is possible that it can survey the simultaneous three-dimensional survey about so many points, therefore it is so fast and precisely. We takes a snap the objects with digital carema, and input the data to the computer from this data. We can take the three-dimensional coordinate through relative orientation, absolute orientation and have to check the merit of three-dimensional suveying. Otherwise we can get the error in one centimeter after analysis three-dimension coordinate and we can get the higher precise accuracy cause to set the camera angle of 20∼45 degrees.

鷄腦脊髓炎感染鷄血淸抗體의 免疫化學的인 性狀

崔源弼 慶北大學校 1977 論文集 Vol.24 No.-

This paper deals with the antibody activities and antibody classes in the chickens infected with avian encephalomyelities (AE) virus. Antibody activities of serum fractions were titerated by indirect flourescent antibody technique(IDFAT) and in part by virus neutralization test (VNT). the classes of antibodies which showed immune activitiy by IDFAT or/and VNT were identified by 0.1 M 2-mercaptoethanol (2-ME) sensitivity test, sucrose density ultracentrifugation, immunoelectrophoresis, immunodiffusion test. The results obtained are summarized as follows: 1) Antibody activities were inactivated by 2-ME treated in the serum at the early immune stage. However, 2-ME sensitive antibody remained for a long time after AE infection. 2) Maternal antibodies in the eggs and 2-day old chickens were identified with IgG which showed 2-ME resistant. 3) Antibody activities of AE antiserum was found in both IgM (16.5∼19S) and IgG(7S). 4) IDFAT and VNT activities were found in both IgM and IgG, respectively. However, IgM was more active to IDFAT than VNT.

鷄腦脊髓炎에 있어서 螢光抗體間接法과 中和試驗의 比較硏究

崔源弼 慶北大學校 1976 論文集 Vol.22 No.-

Authors^7),8) reported on indirect fluorescent antibody technique(IDFAT) to titrate the avian encephalomyelitis(AE) virus antibody of chickens. This paper describes the practical use of IDFAT to detect AE-virus antibodies in chicken flocks and their product, and the comparision of an IDFAT, virus-neutralization test(VNT) and embryo-susceptibility test(EST), 1) In the 323 serum samples(135 birds), all of 233 serum samples with log neutralizing indices(NI) of 1.1 more, showed positive reaction IDFA titer;1:20 to 1:640), and among the 90 serum samples of NI of less than 1.1, 55 samples showed positive reaction of 1:5∼1: 40 by IDFAT. 2) In the 175 serum samples(78birds) of positive IDFAT, the correlation between the results of IDFAT and VNT was demonstrated as a stright line. 3) The serum antibodies were detected in 1 week by IDFAT and the titers increased almost parallel with that of the NI. The highest titer of serum antibodies was deserved in 5∼6 weeks after the inoculation. 4) Evident fouctuations of the antibody titers were found in the observation period of 21∼34 months after the infection in 【certain chickens of AE-infected flocks. Otherwise, serum antibodies disappeared at the 5 and 11 months by VNT, and 16 months by IDFAT after infection. 5) After oral inoculation of AE virus in laying hens, maternal antibodies of eggs appeared in 2 weeks by the IDFAT. But the neutralizing antibody was detected in egg yolks in 3 weeks. 6) All of the sera and chloroform extracts of eggs, which came from AE-vaiccinated chicken flocks, became positve, soon or later, by the IDFAT and VNT. Eighty percent or more resistant embryos were found in the flocks. These data indicate that the IDFAT is a more rapid, accurate and sensible method to an in vitro serological test and recognize the immune status of certain chicken flocks for AE than VNT and EST.

猫의 白癬菌症에 관한 硏究

崔源弼 慶北大學校 1979 論文集 Vol.28 No.-

This experiment was undertaken to determine the causative agent of dermatophytosis occured in the cat. Direct microscopic examination, culture and pathogenicity test were performed for the sample (hair and scale) obtained from the skin lesion of the affected cat. The causative agent was identified as Trichophyton mentagrophytes and the present report describes the first case fo the cat ringworm in Korea.

혼합 유전자형 검출(HLA-DQα, D1S80 and Vwa Loci)에 관한 연구

강필원,박기원,이경룡,최상규 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

국내 동물 유래 Trichophyton속 균의 생물학적 성상 및 Trichophyton mentagrophytes의 아형의 분류

박진희,최원필 한국수의공중보건학회 2002 예방수의학회지 Vol.26 No.4

국내에서 분리된 8종의 Trichophyton속의 생물학적 성상 및 T. mentagrophytes 아형의 분류는 아래와 같았다. BCP-MS-G 배지에서 T.mentagrophytes (60주), T. terrestre (3주)는 알칼리성으로 변하고 잘 자랐으며 T. tubrum (3주), T. raubischekii (2주), T. tonsurans (5주), T. equinum (3주), T. verrucosum (8주)은 알칼리성으로 변하지 않고 잘 자라지 않았다. 특히 T. verrucosum은 배지가 투명해졌으며, T. ajelloi (3주)는 알칼리성으로 변하지 않고 잘 자랐다. Urease 산생 검사에서 T. mentagrophytes, T. raubitschekii. T. tonsurans, T. equinum, t. ajelloi, T. terrestre는 양성이었으며 T. rubrum은 음성이었고, T. verrucosum은 배양 10일 째에는 모두 양성이었다. 모발 천공성 검사에서 T. mentagrophytes, T. tonsurans, T. ajelloi는 양성이었고, T. rubrum, T. raubitschekii, T. equinum, t. terrestre, T. verrucosum은 음성이었다. Trichophyton Ⅰ~Ⅳ배지에서 균 종간에 영양요구성의 차이가 인정되었다 이상의 생물학적 성상 검사는 육안적 및 현미경적으로 구별이 어려운 Trichophyton속 종간의 감별에 유용하였다. T. mentagrophytes 60주는 집락의 육안적 성상의 차이로 과립형 41주 (68.B%), 분말형 10주 (16.7%), 도실형 2주 (3.3%), 융모형 7주 (11.7%)의 4가지 아형으로 나누어졌다. 동물 유래 T. mentagrophytes 57주 중 과립형은 71.9%, 분말형은 17.5%, 도실형은 3.5% 및 융모형은 7%이며 과립형, 분말형 및 도실형은 대분생자, 소분생자 및 나선상 기관의 출현 빈도의 차이가 인정되었고 융모형은 소분생자만 관찰되었다. The present study was conducted to examine biological characteristics of 8 species of Trichophyton (T.) isolated from animals in Korea - T. equinum (3 strains), T. mentagrophytes (60 strains), T. raubitschekii (2 strains), T. rubrum (3 strains), T. tonsurans (5 strains), T. verrucosum (8 strains), T. ajelloi (3 strains), T. terrestre (3 strains) - and to classify subtypes of T. mentagrophytes according to their colony morphology. Biological characteristics on bromocresol purple-milk solids-glucosemedium, urea broth and Trichophyton media and hair perforation test were useful for the identification and differentiation of T. equinum, T. mentagrophytes, T. raubitschekii, T. rubrum and T. tonsurans even though they had similar morphology. Sixty 10 strains of T. mentagrophytes were classified into 4 subtypes: granular type (41 strains, 68.3%), powdery type (10 strains 16.7%), purple-red type (2 strains, 3.3%) and cottony type (7 strains, 11.7%). Granular type was the most common subtype in the strains isolated from animals.

출생 전 스트레스와 감금 스트레스가 흰쥐 편도복합체 별아교세포에 미치는 영향 : I. 별아교세포의 세포체에 미치는 영향

이지용,최병영,김동희,정원석,조병필,양영철 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

신경계통의 가소성은 신경세포의 변화에 의해 이루어질 뿐 아니라 신경아교세포의 변화에 의해서도 이루어진다. 신경아교세포 중 별아교세포는 신경세포의 기능을 조절하므로 정상적인 뇌의 기능을 유지하는데 매우 중요하다. 뇌에서 편도복합체는 위험 혹은 유해한 일련의 감각정보를 받아들이는 구역으로 받아들인 일련의 이와 같은 정보를 통합하고 변환시켜 공포라는 감정을 만들어낸다. 이런 과정은 편도복합체 신경세포에서 분비되는 신경전달물질의 균형변화에 의해 이루어지며, 신경전달물질의 조절에 별아교세포가 관여하므로 본 연구에서는 출생 전 스트레스와 성장 후에 받은 스트레스가 편도복합체 별아교세포의 세포체에 어떤 영향을 미치는지 조사하였다. 이를 위해 흰쥐를 스트레스를 받지 않은 대조군 (CON), 성장 후 스트레스를 받은 군(CONR), 출생 전 스트레스를 받은 군(PNS), 출생 전 스트레스와 성장 후 스트레스를 모두 받은 군 (PNSR)으로 구분하였다. 별아교세포는 GFAP 항체를 이용한 면역조직화학 염색을 시행하여 확인하였으며, methylene blue/azure II로 대조 염색하였다. Neurolucida 프로그램을 이용하여 계측한 별아교세포의 세포체는 일부 편도복합체 신경핵에서 출생 전 스트레스를 받은 PNS군이 대조군에 비하여 면적이 증가하였으며, 이런 경향은 출생전 스트레스를 받고 다시 성장 후 스트레스를 받은 PNSR군에서 더욱 증가하였다. 따라서 흰쥐의 편도복합체에 분포하는 별아교세포는 스트레스에 영향을 받아 비대해지는 경향을 보인 것으로 나타났으며, 출생 전 스트레스가 성장 후에도 영향을 미치는것으로 사료된다. The plasticity of nervous system is generated not only due to changes in neurons but also due to changes in neuroglial cells. Astrocyte is important for maintaining the normal brain function and controlling the neuronal functions. The amygdala receives an array of important sensory information of danger signals. This information is further transduced and integrated to produce the highly adaptive emotion, fear. In this study, morphometric changes in the cell bodies of astrocytes in the amygdala, induced by prenatal stress and restraint stress were examined. For this purpose, rats were classified into 4 groups; control group (CON), only restraint-stressed (starting on P90 for 3 days) group (CONR), prenatally-stressed group (PNS), and prenatally and restraint (on P90 for 3 days) stressed group (PNSR). Astrocytes were verified with anti-GFAP immunohistochemistry, counter stained with methylene blue/azure II and were examined using the Neurolucida. Results showed that astrocytes in the amygdala of PNS rats had significantly larger cell bodies than did CON rats and this was enhanced further by restraint stress. Thus this data showed that hypertrophy of the astrocytic cell bodies of amygdala complex is induced by prenatal and restraint stress.

韓國 在來山羊의 消化管 및 膵臟內 寄生蟲 調査

李鉉凡,李熙碩,崔源弼 慶北大學校 1977 論文集 Vol.24 No.-

Present study was undertaken in order to clarify the species and infection rate of internal parasites in Korean native goats. Abomasum of 100 goats slaughtered in Daejun and abomasum, intestinal tracts, and pancreata of 40 goats slaughtered in Taegu prefecture were inspected. The parasites recovered were classified morphologically with following results. 1) A total of 7 species of parasite was identified. Their habitats and infection rates were as follows; Abomasum-Haemonchus contortus: 20% in Taegu and 42% in Daejun, and Trichostrongylus sp. : 2.5% in Taegu and 3% in Daejun. Small intestine-Moniezia expansa: 12.5%. Large intesine Oesophagostomum columbianum: 27.5%, Oesophagostomum venulosum: 17.5%, and Trichuris ovis: 5%. Pancreas-Eurytrema coelomaticum: 15%. 2) Characteristic nodule formation on the walls of cecum, colon, and ileum was observed in 5 out of 11 goats infected with Oesophagostomum columbianum. No nodule formation, however, was observable in goats infected with Oesophagostomum venulosum. 3) the maximum worm burdens of Oesophagostomum columbianum, Eurytrema coelomaticum, and Haemonchus contortus were higher (above 83) than those of the other parasites. 4) the numbers of female nematodes were greater than of male except Oesophagostomum venulosum.

Cytochrome b 유전자분석법을 이용한 동물의 종 식별

김순희,강필원,백형순,이양한,최상규,한면수 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-