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Gene Cloning and Expression of Cephalosporin-C Deacetylase from Bacillus sp.KCCM10143
Choi, Duk Ho,Kim, Yong Duk,Chung, Il Sun,Lee, Sang Hun,Kang, Sang Mo,Kwon, Tae Jong,Han, Kum Soo 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.2
Cephalosporin-C deacetylase (CAH) catalyzes the deacetylation of cephalosporin derivatives. A novel gene encoding the CAH from Bacillus sp. KCCM10143 was cloned and sequenced. The nucleotide sequence contained an open reading frame encoding a polypeptide consisting of 217 amino acids and a molecular weight of 24kDa which was in good agreement with the value obtained by sodium dodecylsulfate-polyacrylamide gel electrophoresis. An expression plasmid was constructed by inserting the CAH gene into the region of the pTrc99A expression vector. An active form of the CAH protein was expressed in the soluble fraction obtained after cell disruption. In fermentation using a 5-1 jar fermenter, the transformant E. coli JM 109 (pDST654) produced 4.12 U of CAH per ml of culture during 16h of incubation.
Sung, Chang Ohk,Ahn, Geunghwan,Song, Sang Yong,Choi, Yoon-La,Bae, Duk-Soo Ovid Technologies (Wolters Kluwer) - Lippincott Wi 2009 International journal of gynecological pathology Vol.28 No.6
<P>Atypical leiomyomas are characterized by moderately to severely pleomorphic atypical tumor cells with low mitotic counts and no coagulative tumor cell necrosis. Despite the worrisome histologic features, most tumors have shown benign behavior. However, most studied patients had total hysterectomies, and very few patients who had myomectomy alone have had long-term follow-up. The behavior of atypical leiomyomas treated with myomectomy alone is still not known. We present a study of 13 atypical leiomyomas treated with myomectomy alone with long-term follow-up results and immunohistochemical findings for p16 (p16), p53, Ki-67, estrogen receptors, and progesterone receptors. The lengths of the postoperative intervals ranged from 20 to 151 months (mean=76.1 mo). None of the patients had developed a metastasis of her atypical leiomyoma. However, 1 patient showed local recurrence at the past myomectomy site 22 months after initial myomectomy. Immunostaining for p16 showed moderate to strong diffuse immunoreactivity in 11/13 cases (84.6%) of atypical leiomyomas, and 38.5% showed immunoreactivity for p53, including 1 case with strong, diffuse positive p53. When an atypical leiomyoma is discovered in a myomectomy specimen, the patient should be carefully followed up with or hysterectomy could be recommended. Larger numbers of cases and more long-term follow-up studies are needed to confirm the safety of myomectomy for atypical leiomyomas.</P>
Duk-Yeon Cho,Yon-Suk Kim,Sang-Ho Seol,Dong-Kug Choi 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
PL a traditional plant in oriental countries is known for its pharmacological benefits for hemiplegia, blood clots, abdominal pain, rheumatoid arthritis and cold sweat. Here we examined PL ethanol extract would be efficacious in protecting RAW264.7 macrophages from lipopolysaccharide (LPS)-induced stress signaling. RAW264.7 cells were pretreated with an PL ethanol extract prior to exposure to LPS. PL inhibits LPS-induced production of nitric oxide(NO). The mRNA and protein levels of inducible nitric oxide synthase and cyclooxygenase-2 also decreased dose dependently following PL ethanol extract treatment. The production of proinflammatory cytokines such as tumor necrosis factor-α, interleukin-1β and IL-6 were inhibited by suppressing their transcriptional activity. Further mechanistic study revealed that PL ethanol extract suppressed nuclear factor-κB activation by blocking p65 of phosphorylation. Taken together, our findings demonstrate that PL ethanol extract might be developed as a promising candidate to treat various inflammatory disease.
(Sung Hyun Kwon),(Duk Soo Choi),(Dae Chul Cho) 한국물환경학회 2000 한국물환경학회지 Vol.16 No.1
Biodegradability of pentachlorophenol and its biological kinetic parameters were investigated using a chemical oxygen demand (COD) analysis. The experiments were performed in continuous stirred tank reactors with synthetic wastewater containing 30 and 60㎎/L of pentachlorophenol (PCP) and other carbon sources. Mean cell residence time (MCRT) and hydraulic residence time (HRT) were maintained from 4.8 to 26.2 days and 4 to 17 hours, respectively. While synthetic wastewater containing 30 ㎎/L of PCP showed over 90% S-COD removal efficiencies except at the 4.8 and 20.3 days of MCRT, 55% to 75% removal efficiencies were observed for 60 ㎎/L of PCP concentration. The respective values of Y and k_d were 0.15 and 0.010 day^(-1) for 30 ㎎/L PCP (r²= 0.90), and 0.13 and 0.014 day^(-1) for 60 ㎎/L PCP (r² = 0.86), respectively. In a substrate inhibition kinetics, Haldane model, the respective values of K_s, K_i and k were 60.8 ㎎ COD/L, 297.5 ㎎ COD/L and 0.93 day^(-1) for 30 ㎎/L PCP (r² = 0.30), and 262.2 ㎎ COD/L, 997.2 ㎎ COD/L and 0.053 day^(-1) for 60 ㎎/L PCP (r² = 0.78), respectively.
Enhanced Production of Epothilones by Carbon Sources in sorangium cellulosum
( Sang Woo Park ),( Sue Hyung Choi ),( Yeo Joon Yoon ),( Dong Hyun Lee ),( Duk Joon Kim ),( Ji Heung Kim ),( Young Kwan Lee ),( Guang Jin Choi ),( Ick Tae Yeom ),( Sang Jun Sim ) 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.4
( Sung-eun Choi ),( Dong-jin Park ),( Ji-hyoun Kang ),( Kyung-eun Lee ),( Haimuzi Xu ),( Ji Shin Lee ),( Yoo-duk Choi ),( Shin-seok Lee ) 대한류마티스학회 2019 대한류마티스학회지 Vol.26 No.1
Objective. Although intravenous cyclophosphamide (IVC) is generally accepted as the standard therapy for induction treatment of active proliferative lupus nephritis (LN), several clinical trials have suggested that mycophenolate mofetil (MMF) is at least as effective as IVC. Because few Asian studies have compared the two treatment modalities, we compared the efficacies of MMF and IVC as LN remission induction treatments in Korean patients. Methods. We enrolled 39 patients with class III and IV LN who received MMF or IVC as LN induction therapy. The renal outcomes (i.e., complete response [CR], partial response [PR], and no response [NR]) at 6 and 12 months were defined using the ACR 2006 response criteria. Results. Of 39 patients, 23 (59.0%) were treated with IVC, and 16 (41.0%) were treated with MMF. Demographics, clinical characteristics, laboratory data, and adverse events did not significantly differ between the two groups. However, C3 levels were lower and activity scores in renal biopsy were higher in IVC-treated patients. CRs were achieved by 11 (47.8%) of the patients receiving IVC and 7 (43.8%) of the patients receiving MMF after 6 months of treatment (p=0.961) and by 11 (47.8%) of those who received IVC and 9 (56.2%) of those who received MMF at 12 months of treatment (p=0.713). Neither the PR rate nor the NR rate differed significantly at 6 or 12 months between the two groups. Conclusion. The efficacy of MMF does not differ from that of IVC in terms of induction of LN remission in Korean patients. (J Rheum Dis 2019;26:57-65)
Sang Won Jeon,Changsu Han,Young-Hoon Ko,Seo Young Yoon,Chi-Un Pae,Joonho Choi,Yong Chon Park,Jong-Woo Kim,Ho-Kyoung Yoon,Seung-Duk Ko,Ashwin A. Patkar,Mark Zimmerman 대한정신약물학회 2017 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.15 No.1
Objective: This study was aimed at evaluating the diagnostic validity of the Korean version of the Clinically Useful Depression Outcome Scale (CUDOS) with varying follow-up in a typical clinical setting in multiple centers. Methods: In total, 891 psychiatric outpatients were enrolled at the time of their intake appointment. Current diagnostic character-istics were examined using the Structured Clinical Interview for DSM-IV (41% major depressive disorder). The CUDOS was meas-ured and compared with three clinician rating scales and four self-report scales. Results: The CUDOS showed excellent results for internal consistency (Cronbach’s α ,0.91), test-retest reliability (patients at intake, r =0.81; depressed patients in ongoing treatment, r =0.89), and convergent and discriminant validity (measures of depres-sion, r =0.80; measures of anxiety and somatization, r =0.42). The CUDOS had a high ability to discriminate between different levels of depression severity based on the rating of Clinical Global Impression for depression severity and the diagnostic classi-fication of major depression, minor depression, and non-depression. The ability of the CUDOS to identify patients with major depression was high (area under the receiver operating characteristic curve=0.867). A score of 20 as the optimal cutoff point was suggested when screening for major depression using the CUDOS (sensitivity=89.9%, specificity=69.5%). The CUDOS was sensitive to change after antidepressant treatment: patients with greater improvement showed a greater decrease in CUDOS scores ( p <0.001). Conclusion: The results of this multi-site outpatient study found that the Korean version of the CUDOS is a very useful measure-ment for research and for clinical practice.
( Sang Il Choi ),( Joong-won Park ),( Bo Hyun Kim ),( Ju Hee Lee ),( Seung Duk Lee ),( In Joon Lee ),( Tae Hyun Kim ),( Seong Hoon Kim ),( Young Hwan Koh ),( Hyun Beom Kim ),( Eun Kyung Hong ),( Chang 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: In South Korea, an endemic area of hepatitis B virus (HBV), the incidence and mortality of HCC is gradually decreasing by HBV vaccination, antiviral treatment, early tumor detection, improved treatment options, and evidence-based guidelines. This study investigated the changes over time in the baseline characteristics and clinical outcomes of HCC patients at a single center. Methods: Patients initially diagnosed with and treated for HCC at the National Cancer Center between 2000 and 2015 (n=4291) were followed-up until February 2017. Difference in patient characteristics and outcomes were compared in patients diagnosed in 2000-2004 (n=1157), 2005-2009 (n=1678), and 2010-2015 (n=1456). Results: The median age of the patient cohort was 57 years (range 13-98) years, and most were male (3502, 81.6%). HBV infection was the most common etiology (74.7%). The percentage of patients with Child class A (69.1%, 79.9%, and 87.1%, respectively) and BCLC stage 0 (4.1%, 5.2%, and 9.8%, respectively) increased significantly over time. Median overall survival also increased significantly over time, from 14.4 months (95% confidence interval [CI] 12.0-16.8 months) in 2000-2004, to 22.9 months (95% CI, 20.3-25.5 months) in 2005-2009, to 53.6 months (95% CI, 45.7- 61.5 months) in 2010-2015 (p<0.001). OS of patients with modified UICC stage IV (3.6 vs. 4.7 vs 6.1 months, p<0.001) and with HBV etiology (12.7 vs 20.4 vs 64.5 months, p<0.001) was observed in patients diagnosed in 2000-2004, 2005-2009 and 2010-2015, respectively. Conclusions: Survival of patients with HCC has improved significantly over time in a single center in South Korea. Analysis is ongoing to determine the causes of the improved outcomes.
( Sang Duk Ji ),( Gyoo Byung Sung ),( Pil Don Kang ),( Kee Young Kim ),( Yong Soo Choi ),( Nam Suk Kim ),( Soon Ok Woo ),( Sang Mi Han ),( In Pyo Hong ),( Nam Gyu Ha ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.3
This study was conducted to confirm the possible use of female Yangwonjam as a host for synnemata production of Isaria tenuipes in eight local areas in Korea. Silkworm pupation rate, infection rate and synnemata characteristics of I. tenuipes were examined. Normal silkworms had a higher pupation rate than silkworms inoculated with I. tenuipes. The pupae survival percentage of normal silkworm in cocoons was 92.5~97.6%, whereas it ranged from 91.1~95.6% in silkworms sprayed with I. tenuipes. Female Yangwonjam showed the highest survival percentage at 97.6% among the silkworm varieties tested. I. tenuipes infection rate of larvae of 5th instar newly-exuviated silkworms was 89.2~90.7% in the spring rearing season and 98.2~99.3% in the autumn rearing season. Synnemata production of I. tenuipes was excellent in female Yangwonjam with an incidence rate of 98.0% followed by male Yangwonjam (94.1%) and Baegokjam (93.3%) in the spring rearing season. Synnemata living weight ranged from 1.44~0.94 g in the spring rearing season. The female Yangwonjam had the heaviest synnemata weight (1.44 g) in the spring rearing season. The synnemata of I. tenuipes produced on pupae were white or milky-white in color, and were similar in shape and color to wild synnemata collected in Korea.
( Duk Hwan Kim ),( Bun Kim ),( Jae Hyuk Choi ),( Soo Jung Park ),( Sung Pil Hong ),( Tae Il Kim ),( Won Ho Kim ),( Jae Hee Cheon ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Fecal calprotectin (FC) concentration directly represents the degree of intestinal infi ammation. It is established that FC level predicts the clinical course of in- fi ammatory bowel disease. However, little is known about the impact of FC in patientswith intestinal Behcet`s disease (BD). Methods: fifteen consecutive patients with systemic BD who undertook colonoscopy for evaluation of gastrointestinal symptoms were prospectively enrolled between November, 2012 and March, 2013 in Severance hospital, Seoul, Korea. Fecal specimens from the patients were obtained one day before starting bowel preparation. FC level was compared with colonoscopic outcomes, disease activity index for intestinal BD (DAIBD), and laboratory markers. Results: Median age of the patients was 43 (31-68) and nine (60%) were male. Of them, 11 (73.3%) showed intestinal ulcers (fi ve typical and six atypical ulcers). Terminal ileum was the most frequent location (81.8%). Three defi nite intestinal BD (27.3%) and eight probable intestinal BD (72.7%) were diagnosed from the established criteria. Median FC level in patients who had typical intestinal ulcers was signifi cantly higher than in those with atypical ulcers or without ulcers (567.83 μg/g (327.12-1604.39), 51.75 μg/g (20.14-95.18) and 58.36 μg/g (6.04-103.53), respectively; P=0.004 and 0.016, respectively). However, serum CRP level and DAIBD in patients with typical ulcers were not signifi cantly different from those in patients with atypical ulcers or without ulcers. Conclusions: High FC level was clearly correlated with typical intestinal BD ulcers. FC level might have a signifi cant role as a non-invasive surrogate marker of intestinal involvement of BD.