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Park, Ky-Yeon,Lee, Jin-Sook,Choi, Soon-Young,Cho, Dae-Ho,Cheon, Choong-Ill,Song, Eunsook,Min, Kyung-Hee,Chung, Hyunk,Lee, Heung-Sick,Lee, Myeong-Sok 한국유전학회 2003 Genes & Genomics Vol.25 No.4
The aroD gene was cloned from Corynebacterium glutamicum by complementation of Escherichia coli aromatic amimo acid auxotroph. The E. coli aroD gene codes for 3-dehydroquinte dehydratase (dehydroquinase), an enzyme involved in the aromatic amino acid biosynthesis pathway. The cloned insert DNA fragments in the recombinant plasmids, named pROD5, pROD8, and pROD14, were physically mapped with several restriction endonucleases. We determined the DNA nucleotide sequences of the fragment and reported here the sequences of the aroD gene. The complete nucleotide sequences of the aroD gene showed an open reading frame encoding a protein of 145 amino acids with a deduced molecular weight of about 16 kDa. Comparison of this deduced amino acid sequences of C. glutamicum aroD gene with those of the enzyme from Pseudomonas aeruginosa, Mycobacterium tuberculosis and Actinobacillus pleuropneumonia revealed 51%, 53% and 54% similarity, respectively.
Su-Jin Jang,Ky Young Park,Gwang-Yeon Gi,Kyong-Ju Choi,Sang Ki Choi,Yong-Bum Kim 한국원예학회 2006 Horticulture, Environment, and Biotechnology Vol.47 No.3
To examine the possible involvement of polyamines on the postharvest longevity of cut rose, the endogenous polyamine levels, ethylene production, the effect of polyamine on the postharvest longevity and the change of S-adenosylmethionine decarboxylase (SAMDC) were analyzed in the cut rose flowers and leaves. Putrescine was the major product among analyzed polyamines in flowers and leaves in two different cut rose cultivars, ‘Cardinal’ and ‘Vital’, and rapidly decreased in 3 days. The exogenous spermine among polyamines (putrescine, spermidine, and spermine) could effectively induce the postharvest longevity of the cut roses. In addition, the incubation of the cut flower with water increased both SAMDC activity and spermine content by 2 times, which were followed by ethylene production. The exogenous polyamines suppressed ethylene production or delayed the onset of the ethylene production. These results provide evidence that spermine may be able to increase the longevity of cut rose. Additionally, the increase of the endogenous spermine with water results from enhancing SAMDC activity. Further, we discuss that the increase of spermine content and SAMDC activity may play role as a resistance mechanism against fungal and bacterial infection and the development of transgenic rose overexpressing SAMDC may be a useful tool to improve the current rose cultivars for the commercial usage.
The expression of ketohexokinase is diminished in human clear cell type of renal cell carcinoma
Hwa, Jeong Seok,Kim, Hyun Joon,Goo, Bo Mee,Park, Hyo Jin,Kim, Choong Won,Chung, Ky Hyun,Park, Hyung Chul,Chang, Se-Ho,Kim, Yeon Woong,Kim, Deok Ryong,Cho, Gyeong Jae,Choi, Wan Sung,Kang, Kee Ryeon WILEY-VCH Verlag 2006 Proteomics Vol.6 No.3
<P>For identification and targeting of tumor-associated marker proteins, the proteome of clear cell type of renal cell carcinoma (RCC) and normal kidney tissues was analyzed by 2-DE. Ketohexokinase (also called fructokinase), which catalyzes the phosphorylation of fructose to fructose 1-phosphate, was identified by MALDI-TOF MS and found to be expressed at low rates in the renal tumor tissues. We found a decreased amount of ketohexokinase mRNA in RCC compared to that observed in the normal kidney tissues by Northern blot. The activity of ketohexokinase in 20 clear cell RCC specimens and the 20 corresponding normal kidneys was investigated, and its activity was shown to be approximately 1.4-fold lower in the RCC specimens than in the normal kidney. Ketohexokinase activity in tumor stage pT3 RCC was 1.5-fold lower than in pT1 RCC. The level of ketohexokinase activity in histological grade 3 RCC was 1.8-fold lower than that in grade 1 cancer. In addition, using in situ hybridization, it was revealed that ketohexokinase in the normal kidney tissue was confined to the proximal tubular epithelial cells, while the expression of ketohexokinase in RCC tissues was extremely low. Our research results show that the expression of human ketohexokinase was diminished in clear cell RCC.</P>
이선형,원종건,최장수,안덕종,최기연,이우경,박소득,손재근,Lee Sun Hyung,Won Jong Gun,Choi Jang Soo,Ahn Duok Jong,Choi Ky Yeon,Lee Woo Gyeong,Park So Deuk,Son Jae Keun 한국작물학회 2005 Korean journal of crop science Vol.50 No.suppl1
This study was carried out to provide the geographic information based on the distribution of rice qualities and palatability in Gyeoungbuk province of Korea. The rice grain quality and environmental factors were analyzed using 513 sampling sites based on different five-agroclimtic regions of Gyeoungbuk province during three years from 2002 to 2004. In rice grain quality characteristics, the average palatability was low in South eastern coastal and Tabaek semi alpine regions as $67.6\~68.3$ and the coefficient of variation (CV) was relatively high as $6.2\~7.4\%$. The average head rice rates were low in South and Central eastern coastal regions as $87.3\~88.2\%$ and CV was high as $8.2\~8.3\%$. The average protein content was high in Central eastern coastal regions as $8.0\%$ and CV was high as $8.2\~8.3\%$. In case of palatability, the variation was differed clearly between high and low agroclimatic regions; it means that it is possible to divide the same agroclimatic region of high CV into two or three areas by CV of palatability. As the results of subdividing each existing agroclimatic regions based on the palatability, the variation of grain quality characteristics was become lower than that of existing five-agroclimatic regions. Therefore, the re-establishing of agroclimatic region based on rice grain quality was very important for precise cultivation for rice. 경북 지역 농업기후 지대를 미질 및 식미치를 근거로 하여 다시 세분화된 지대를 설정하고자 2002년부터 2004년까지 3년간 경북 전 지역에서 가장 많이 재배되고 있는 품종 중 중만생종인 일품벼, 주남벼, 남평벼, 중생종인 화영벼, 조생종인 오대벼를 재배하고 있는 논 513개소의 쌀을 수집하여 분석 조사한 결과를 요약하면 다음과 같다. 1. 지대별 식미치의 평균은 동해안 남부지대가 67.6로 다른 지대에 비해 약간 낮았으며, 완전미 비율은 동해안 남부지대 및 동해안 중부지대가 각각 $87.3\%,\;88.2\%$로 낮았으며 변이계수도 각각 $8.2\~8.3\%$로 높았다. 단백질 함량은 동해안 중부지대가 $8.0\%$로 다른 지대에 비해 약간 높았고, 변이계수도 $8.8\%$ 높았다. 2. 특정지역을 세분하여 쌀 품질을 조사해보면 전체 평균의 변이계수보다 높게 나타나는 지역과 낮게 나타나는 지역으로 뚜렷하게 구분된다. 변이 계수가 크다는 것은 특정 지역 내에서도 쌀 품질의 세분화된 지역간 차이가 심하므로 고품질 쌀 생산을 위해서는 세분화된 벼 재배 구역 설정하여 적지적작의 정밀농업이 필요하였다. 3. 식미치를 근거로 세분화된 지대의 쌀 품질 요인에 대한 연차 간 변이계수는 기존 농업기후지대의 변이계수보다 상당히 낮아져, 세분화된 지대별 고품질 쌀 생산을 위한 특정 지역의 정밀 기술 개발 및 보급의 기초 자료로 활용할 수 있을 것으로 사료되었다.