http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Su-Hyun Jo,Se-Young Choi,Ji-Hyun Yun,Young-Sang Koh,Won-Kyung Ho,Chin O. Lee 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.4
We investigated the effects of trinitrobenzene sulfonic acid (TNBS), an amino-group reagent, on the human ether-a-go-go-related gene (HERG) K+ channels expressed in Xenopus oocytes. TNBS neutralizes the positively charged amino-groups of peptide N-terminal and lysine residues. External application of TNBS at 10 mM for 5 min irreversibly shifted the curves for currents at the end of the pulse and tail currents of HERG to a more negative potential and decreased the maximal amplitude of the Itail curve (Itail,max). TNBS had little effect on either the activated current-voltage relationship or the reversal potential of HERG current, indicating that TNBS did not change ion selectivity properties. TNBS shifted the time constant curves of both activation and deactivation of the HERG current to a more hyperpolarized potential; TNBS’s effect was greater on channel opening than channel closing. External H+ is known to inhibit HERG current by shifting V1/2 to the right and decreasing Itail,max. TNBS enhanced the blockade of external H+ by exaggerating the effect of H+ on Itail,max, not on V1/2. Our data provide evidence for the presence of essential amino-groups that are associated with the normal functioning of the HERG channel and evidence that these groups modify the blocking effect of external H+ on the current.
Jo Su-Hyun,Choi Se-Young,Yun Ji-Hyun,Koh Young-Sang,Ho Won-Kyung,Lee Chin-O. The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.4
We investigated the effects of trinitrobenzene sulfonic acid (TNBS), an amino-group reagent, on the human ether-a-go-go-related gene (HERG) $K^+$ channels expressed in Xenopus oocytes. TNBS neutralizes the positively charged amino-groups of peptide N-terminal and lysine residues. External application of TNBS at 10 mM for 5 min irreversibly shifted the curves for currents at the end of the pulse and tail currents of HERG to a more negative potential and decreased the maximal amplitude of the $I_{tail}$ curve $(I_{tail,max})$. TNBS had little effect on either the activated current-voltage relationship or the reversal potential of HERG current, indicating that TNBS did not change ion selectivity properties. TNBS shifted the time constant curves of both activation and deactivation of the HERG current to a more hyperpolarized potential; TNBS's effect was greater on channel opening than channel closing. External $H^+$ is known to inhibit HERG current by shifting $V_{1/2}$ to the right and decreasing $I_{tail,max}$. TNBS enhanced the blockade of external $H^+$ by exaggerating the effect of $H^+$ on $I_{tail,max}$, not on $V_{1/2}$. Our data provide evidence for the presence of essential amino-groups that are associated with the normal functioning of the HERG channel and evidence that these groups modify the blocking effect of external $H^+$ on the current.
Yoon Kyung Cho,Chin Su Koh,Youjin Lee,Minkyung Park,Tae Jun Kim,Hyun Ho Jung,Jin Woo Chang,Sang Beom Jun 대한의용생체공학회 2023 Biomedical Engineering Letters (BMEL) Vol.13 No.1
Brain–machine interface (BMI) provides an alternative route for controlling an external device with one’s intention. For individuals with motor-related disability, the BMI technologies can be used to replace or restore motor functions. Therefore, BMIs for movement restoration generally decode the neural activity from the motor-related brain regions. In this study, however, we designed a BMI system that uses sensory-related neural signals for BMI combined with electrical stimulation for reward. Four-channel electrocorticographic (ECoG) signals were recorded from the whisker-related somatosensory cortex of rats and converted to extract the BMI signals to control the one-dimensional movement of a dot on the screen. At the same time, we used operant conditioning with electrical stimulation on medial forebrain bundle (MFB), which provides a virtual reward to motivate the rat to move the dot towards the desired center region. The BMI task training was performed for 7 days with ECoG recording and MFB stimulation. Animals successfully learned to move the dot location to the desired position using S1BF neural activity. This study successfully demonstrated that it is feasible to utilize the neural signals from the whisker somatosensory cortex for BMI system. In addition, the MFB electrical stimulation is effective for rats to learn the behavioral task for BMI.
Lee, Hyun Joo,Nam, Yunjun,Koh, Chin Su,Im, Changkyun,Seo, In Seok,Choi, Seungjin,Shin, Hyung-Cheul The Korean Society for Brain and Neural Science 2011 Experimental Neurobiology Vol.20 No.4
<P>In this study, we characterize the hemodynamic changes in the main olfactory bulb of anesthetized Sprague-Dawley (SD) rats with near-infrared spectroscopy (NIRS, ISS Imagent) during presentation of two different odorants. Odorants were presented for 10 seconds with clean air via an automatic odor stimulator. Odorants are: (i) plain air as a reference (Blank), (ii) 2-Heptanone (HEP), (iii) Isopropylbenzene (IB). Our results indicated that a plain air did not cause any change in the concentrations of oxygenated (Δ[HbO<SUB>2</SUB>]) and deoxygenated hemoglobin (Δ[Hbr]), but HEP and IB induced strong changes. Furthermore, these odor-specific changes had regional differences within the MOB. Our results suggest that NIRS technology might be a useful tool to identify of various odorants in a non-invasive manner using animals which has a superb olfactory system.</P>
Age-associated changes in pancreatic exocrine secretion of the isolated perfused rat pancreas
Zheng-er Jiang,ChengZhe Jiang,Baihui Chen,Chin Su Koh,Jun-Hwan Yong,Dae-Hun Park,Moo-Ho Won,Yun-Lyul Lee 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.1
Gut functions, such as gastrointestinal motility, gastric secretion and pancreatic secretion, were reduced with age. Glucose tolerance is impaired, and the release of insulin and β-cell"s sensitivity on glucose are reduced with age. However, a lot of controversial data have been reported as insulin concentrations after glucose ingestion are either higher or no different in elderly and young subjects. Thus, this study was aimed to investigate whether aging could affect pancreatic exocrine secretion and its action mechanisms. An isolated perfused rat pancreatic model was used to exclude the effects of external nerves or hormones. Pancreatic secretion was increased by CCK under 5.6 mM glucose background in the isolated perfused pancreas of young (3 months), 12 months and 18 months aged rats. There was no significant difference between young and aged rats. In 3 months old rats, CCK-stimulated pancreatic secretion was potentiated under 18 mM glucose background. However, the potentiation effects of endogenous insulin and CCK were not observed in 12 and 18 months old rats. Exogenous insulin also potentiated CCKstimulated pancreatic secretion in 3 months old rats. Similarly, exogenous insulin failed to potentiate CCK-stimulated pancreatic secretion as that of 3 months old rats. Wet weight of pancreas and amylase content in pancreatic tissue were not changed with age. These results indicate that pancreatic exocrine secretion is reduced with age and endogenous insulin secretion and/or action is involved in this phenomenon.
이경숙(Lee, Kyung-Sook),고진강(Koh, Chin-Kang),김주현(Kim, Joo Hyun),손행미(Son, Haeng-Mi),송미령(Song, Mi Ryeong),유수정(Yu, Su Jeong),조경숙(Cho, Kyung Sook) 대한근관절건강학회 2013 근관절건강학회지 Vol.20 No.2
Purpose: This purpose of study was to examine the degree of the depression and to identify the affecting factors on the depression of Korean female university students. Methods: A cross-sectional descriptive study design was used. Data were collected from 448 female university students. Participants completed a self-reported questionnaire including general characteristics, depression, well-being, stress, self-efficacy, social support, coping, self-esteem, and temperament. Results: The mean of Beck’s Depression Inventory was 11.07. The affecting factors on the depression of female university students were stress, well-being, self-esteem, health status and major. The proportion of variance explained by these affecting factors was 50.0%. Conclusion: Depression among female university students were associated with various factors. Considering these factors, intervention programs should be developed to reduce depression of female university students.
Decoding intravesical pressure from local field potentials in rat lumbosacral spinal cord
Im, Changkyun,Park, Hae Yong,Koh, Chin Su,Ryu, Sang Baek,Seo, In Seok,Kim, Yong Jung,Kim, Kyung Hwan,Shin, Hyung-Cheul IOP 2016 Journal of neural engineering Vol.13 No.5
<P>Chronic monitoring of intravesical pressure is required to detect the onset of intravesical hypertension and the progression of a more severe condition. Recent reports demonstrate the bladder state can be monitored from the spiking activity of the dorsal root ganglia or lumbosacral spinal cord. However, one of the most serious challenges for these methods is the difficulty of sustained spike signal acquisition due to the high-electrode-location-sensitivity of spikes or neuro-degeneration. Alternatively, it has been demonstrated that local field potential recordings are less affected by encapsulation reactions or electrode location changes. Here, we hypothesized that local field potential (LFP) from the lumbosacral dorsal horn may provide information concerning the intravesical pressure. LFP and spike activities were simultaneously recorded from the lumbosacral spinal cord of anesthetized rats during bladder filling. The results show that the LFP activities carry significant information about intravesical pressure along with spiking activities. Importantly, the intravesical pressure is decoded from the power in high-frequency bands (83.9–256 Hz) with a substantial performance similar to that of the spike train decoding. These findings demonstrate that high-frequency LFP activity can be an alternative intravesical pressure monitoring signal, which could lead to a proper closed loop system for urinary control.</P>
Lee, Yunjoo,Choi, Min-Seon,Lee, Gileung,Jang, Su,Yoon, Mi-Ra,Kim, Backki,Piao, Rihua,Woo, Mi-Ok,Chin, Joong Hyoun,Koh, Hee-Jong Springer US 2017 Rice Vol.10 No.-
<P><B>Background</B></P><P>Starch biosynthesis is one of the most important pathways that determine both grain quality and yield in rice (<I>Oryza sativa</I> L.). Sugary endosperm, <I>sugary-1</I> (<I>sug-1</I>), is a mutant trait for starch biosynthesis. Rice plants carrying <I>sug-1</I> produce grains that accumulate water-soluble carbohydrates instead of starch, even after maturity. Although this trait enhances the diversity of grain quality, sugary endosperm rice has hardly been commercialized due to the severely wrinkled grains and subsequent problems in milling. This study was conducted to identify the genes responsible for the <I>sug-h</I> phenotype through a map-based cloning technology.</P><P><B>Results</B></P><P>We induced a mild sugary mutant, <I>sugary-h</I> (<I>sug-h</I>) through the chemical mutagenesis on the Korean <I>japonica</I> cultivar Hwacheong. Grains of the <I>sug-h</I> mutant were translucent and amber-colored, and the endosperm appeared less wrinkled than <I>sug-1</I>, whereas the soluble sugar content was fairly high. These characteristics confer greater marketability to the <I>sug-h</I> mutant. Genetic analyses indicated that the <I>sug-h</I> mutant phenotype was controlled by a complementary interaction of two recessive genes, <I>Isoamylase1</I> (<I>OsISA1</I>), which was reported previously, and <I>Starch branching enzyme IIa</I> (<I>OsBEIIa</I>), which was newly identified in this study. Complementation tests indicated that <I>OsBEIIa</I> regulated the properties of sugary endosperm<I>.</I></P><P><B>Conclusions</B></P><P>Complementary interactions between the starch biosynthesis genes <I>OsISA1</I> and <I>OsBEIIa</I> determine the mild sugary endosperm mutant, <I>sugary-h</I>, in rice. Our finding may facilitate the breeding of sugaryendosperm rice for commercial benefit.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s12284-017-0172-3) contains supplementary material, which is available to authorized users.</P>