위의 상피성 종양에서 p53 암억제 유전자 및 EB 바이러스 RNA의 발현에 관한 연구

원치규,이중달 한양대학교 의과대학 1996 한양의대 학술지 Vol.16 No.2

Background: Mutant p53 gene and Epstein-Barr virus genome (EBER) are expressed in adenocarcinoma cells of the stomach, and their biologic significance involving in gastric carcinogenesis is evaluated. Method: A variety of precancerous and cancerous lesions including intestinal metaplasia(three cases), dysplasia(four cases), adenoma(three cases) and adenocarcinoma(11 cases) were investigated in formalin-fixed, paraffin-embedded tissue sections. Immunohistochemical reaction using monoclonal antibody for p53 gene and in situ hybridization using anti-sense probe for EBER were studied. Results: One of three adenomas and two of 11 adenocarcinomas expressed the p53, and EBER were expressed in one of 11 adenocarcinomas. Conclusions: Relaiton between their overexpression and histologic type of gastric adenocarcinomas was not found. Since the p53 and EBER wer overexpressed in different cases, their cooperative action involving gastric carcinogenesis was believed to be not present.

Girder의 강성을 고려한 복합 재료 교량 상판의 구조 거동

원치문,박제선,이정호,심도식 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-

Many of the bidge and building floor systems, including the girders and cross-beams, also behave a similar special orthotropic plates. Such plates are subject to the concentrate masses in the form of traffic loads, or the test equipments such as the accelerator in addition to their own masses. Analysis of such problems is usually very difficult. Most of the bridge slabs on girders have large aspect ratios. Finite difference method is used for this purpose, in this paper. The reuslt is compared with that of the beam theory.

정착장치를 이용한 RC보의 탄소섬유 휨보강 효과

원치문 한라대학교 2007 論文集 Vol.10 No.-

This paper presents the results of a test program for flexural strengthening characteristics of continuous unidirectional flexible carbon-fiber sheets(CFS) bonded or/and developed to reinforced concrete(RC) beams. A total of three 300 mm × 500 mm × 6,400 mm concrete beams were tested. Beam without any strengthening, beam bonded with CFS, and beam bonded and developed with CFS using development device were studied to determine their effects on flexural strength of the beams. From the tests, it was found that the strength increases with the development of sheets at development device. Among the three beams, beam bonded and developed with CFS using development device provided the most effective strengthening for concrete beam. Beam strengthened using this scheme showed 97.8% increase in flexural capacity as compared to the control beam without any strengthening.

안면신경초종의 수술적 치료

이원상,지장훈,전영명,박기현 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

Efficient Plant Regeneration from Shoot Tip and Young Leaf in Rhodiola sachalinensis A. Bor.

Chi,Hyung-Joon,Yoon,Jae-Ho,Yang,Deok-Chun,Song,Won-Seob 한국자원식물학회 2003 Plant Resources Vol.6 No.3

The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.

흰쥐 적출간 관류법을 이용한 벤지딘계 염료 Direct Black 38의 대사

원종욱,노재훈,김춘성,박윤정,김치년 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.1

Direct Black 38, a kind of benzidine-based azo dye, is widely used as a dye for fabric, leather, cotton, cellulosic material, paper, wool, silk, and so on. Benzidine-based azo dyes are proven as a mutagen and linked to bladder cancer. In 1978, National Institute for Occupational safety and Health recommended that three widely used benzidine-based dyes(Direct Black 38, Direct Blue 6, and Direct Brown 95) should be treated as carcinogens. In this experiment, metabolism of the benzidine-based dye, Direct Black 38 was examined by using an isolated liver perfusion system. To measure the metbolites of Direct Black 38, 8.0μM, 30.5μM and 63,3μM of Direct Black 38 was added into the recirculating perfusate of the isolated perfused rat liver. Samples were collected at 0, 10, 20, 30, 60, 90, 120 minute. They were treated with sep-pak and methanol, and the metabolites were detected and quantified with high performance liquid chromatography(HPLC). Residual non-reactive dye in the perfusate and liver was reduced to benezidine and then analyzed by HPLC. Detected metabolites of Direct Black 38 were benzidine, N-acetybenzidine, and N,N'-diacetylbenzidine. The average concentration of benzidine was 0.1μM and this concentration was maintained throughout the experimental period. The average concentration of Nacetylbenzidine was 0.22μM and took the same pattern of benzidine. When 30.5μM of Direct Black 38 was added to the perfusate, only N,N'-diacetylbenzidine increased slightly with time. From the above results we suggest that only small amount of Dirst Black 38 might be metabolized to benzidine regardless of the s, amount of the Direct Black 38. There are some possible explanations. The liver was damaged during the perparation. And the function of the perfused liver decreased rapidly because adsorbing of dye. The benzidine, acetybezidine and diacetylbenzidine metabolized to other metabolites were not detected in this experiment.

차량용 디젤 기관의 연소에서 알콜첨가의 영향에 관한 연구

김치원,김판호,우학남 경남대학교 신소재연구소 1997 論文集 Vol.9 No.-

현재 차량용 동력발생장치로써 연료절약과 배기가스의 문제 때문에 압축점화 디젤기관이 승용차 시장분야에서 뿐만아니라 대형트럭과 선박엔진의 시장에서 관심이 고조되고 있다. 본 연구에서는 메탄올이 터보 과급기 및 중간냉각기 부착 디젤기관에서 매연배출을 감소시키는 데 유용할 뿐만아니라 출력 및 연소성능을 향상시키는데 현저한 효과가 있음을 확인하였으며 바람직한 결과를 얻었다. For the power generation of the new vehicle engine, the share of the compression ignition Diesel engine increases in the ppassenger car market as well as their more traditional use for the heavy duty trucks and marine engines. In the persent study, Methyl alcohol is a very useful not only for reducing the smoke concentration of Diesel engine but also in Turbo intercooler charged engine for improving the efficiency of the combustion and power.

메탄올-가솔린 混合燃料의 使用시 機關牲態에 미치는 因子에 관한 硏究

김치원 慶南大學校 附設 工業技術硏究所 1990 硏究論文集 Vol.8 No.-

本 硏究에서는 機關性能 및 排氣組成에 미치는 여러 因子들의 거동과 그 效果에 대한 解析을 하고자 電氣點火機關을 대상으로 사이클 시뮤레이션을 통하여 實驗的 硏究를 행하였다. C₂H?OH와 CH₄O 成分의 混合燃料를 燃燒시킬때 機關의 出力과 燃燒特性에 영향을 주는 因子에 관하여 實驗硏究하기 위하여 4氣筒 4行程 가솔린기관, 排氣 및 燃燒分析장치가 사용되었다. With many of the hydrocarbon fuels utilized in spark ignition automotive engine, a specific ratio of air to fuel is required for maximum preformance. SI engine tests, with computerized at data acquisition, of ethanol and methanol fuel blends were conducted to identify engine operating conditions and fuel compositions. In this paper, fuel blends(volume precentages) respectively in 4 cylinders and 4 cycle gasoline engine are examined experimentally. As the significant result, this study describes which determines the quantative effect of engine operating parameters and fuel blends on power output and combustion characteristics in SI automotive internal combustion engine.

혼합연료 분사제어 방식에 의한 연비 및 배기저감에 관한 연구

김치원 경남대학교 환경문제연구소 1997 환경연구 Vol.20 No.-

Diesel engines, because of better fuel economy, are rapidly gaining a larger share of the market for passenger car application in addition to their more traditional use for the heavy duty trucks and marine engines. In this experimental study. Methanol is a very useful not only reduce the smoke concentration of Diesel engine, but also replace the diesel fuel has been used in Turbo intercooler charged engine for analyzing combustion characteristics, engine performance and exhaust emission. Mostly, the problem of exhaust emission from compression ignition engine has been focused mainly in the view point of environment.

제한된 Bin Packing Problem의 유전자알고리즘 해법

장재원,한치근 경희대학교 산학협력기술연구원 1998 산학협력기술연구논문집 Vol.4 No.-

The Bin Packing Problem is to minimize the numper of bins in which a set of items are inserted. The Constrained Bin Packing Problem(CBPP) has a constraint that no two items with the same attribute are packed to a bin. CBPP has been shown to be NP-Complete. To solve CBPP, in this paper, we try to find a near optimal soultion using Genetic Algorithm. But, when applying general Genetic Algorithm to CBPP, the solution may not satisfy the feasibility because genetic operators used to manipulate the chromosomes yield infeasible offsprings. Hence, to solve CBPP, we used a Repair Operator to generate feasible choromosomes in the algorithm.