煎湯에 의한 黃芩 成分 移行率에 관한 硏究I

고병섭,주혜정,마진열,박갑주,안상우 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

In order to improve the qualities of boiling extract the availability of standard compounds was investigated in extract of Scutellariae Radix. The standard compounds used baicalin(1), baicalein(2), and wogonin(3). The availabilities were analyzed by thin layer chromatography(TLC) and high performance liquid chromatography(HPLC) on C18 column. The extract of RS-4(W/V=10g/100ml) showed the highest availabilities as 7.95% of baicalin(1), 1.04% of baicalein(2), and 0.31% of wogonin(3).

多樣한 黃芩藥鍼製劑의 安全性 및 效能에 關한 硏究

김호경,마진열,전원경,윤수영,강은정,주혜정,고병섭 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

In order to detect the safety and effect of various aqua-acupunctures from Scutellariae Radix, the modifications of boiling, filtration and dilution were employed for the manufacture of aqua-acupunctures. We injected 0.2cc of aqua-acupunctures into Joksamri(足三里) of rat, repeatedly. we compared subacute toxicity of them with saline group, distilled water(D.W.) group, acupuncture group and control group. The results were summarized as follows: 1. The groups were all healthy and alive, and there was no special abnormality in physical condition and autopsy. And there were not any toxic symptoms in repeating application of aqua-acupunctures to the rat, including changes of body weight, organ weight, haematological examination and serum biochemical test. 2. There was slight change of body weight in acupuncture group : We could see significance after 3 days (p<0.05) and after 7 days (p<0.001) in body weight loss. After 9 days, all tested groups were suppressed in body weight increment. 3. Result of organ weight: In Palking aqua-acupuncture(D-2 group), saline group and acupuncture group there were some statistical significance. Especially, acupuncture group revealed significant result in liver and spleen than aqua-acupunctures. From this result, we could suggest that the efficacy of acupuncture was preceded herbal medicine. 4. In serum biochemical test, we examined glucose(GLU), triglyceride(TG) and cholesterol(CHOL). In comparison with control group, the diluted 10 times of hwanggum aqua-acupuncture (× 10 group) was recognized significant decrease of glucose, but the diluted 100 times of Hwanggum aqua-acupuncture (× 100 group), D-2 group, saline group were confirmed significant increment. There was not any meaningful change of CHOL in all of tested group, excepting the acupuncture group was exhibited statistically significant decrease(p<0.05). In TG level all tested group except complex injection of standard compound (CPA group) and HG, there were significant value in statistically. The diluted solution was more significant decrease than Hwanggaum aqua-acupuncture(HG). The mutual relationship of components of aqua-acupuncture tended to decrease level of TG, regardless of its concentration. In acupuncture group, we gained some interesting result in meaningful decrease in TG. 5. Haematological examination showed significant increment of gramulocytes(GR) in all tested groups except Hwanggum aqua-acupuncture. And the diluted solutions of HG expressed very high increment of them(p<0.001). The GR and Mean Corpuscular Volume(MCV) of acupuncture group showed statistical significance.

Ojayeonjonghwan, an oriental medicine composed of five seeds, protects against vasomotor and neurological disorders in estrogen-deficient rats

Ko, Byoung-Seob,Ryuk, Jin Ah,Hwang, Joo Tae,Zhang, Ting,Wu, Xuangao,Park, Sunmin SAGE Publications 2019 Experimental biology and medicine Vol.244 No.3

<P> The different ojayeonjonghwan remedies all contain five fruit and seed water extracts, and they have been used for reproductive health in men and women. We hypothesized that the two OJa remedies would differently improve the early menopause-related vasomotor and neurological symptoms in estrogen-deficient animals. Ovariectomized (OVX) rats had either 0.5% dextrin (OVX-control), conjugated equine estrogen (150 μg/kg body weight; positive-control), 0.5% ojayeonjonghwan remedy-1 (OJa1), or 0.5% ojayeonjonghwan remedy-2(OJa2) in high-fat diet for 12 weeks. Normal-control rats (sham operation) were fed the same high-fat diet as OVX-control rats. Tail skin temperature, depressiveness, memory function, and body composition were determined. The mRNA expressions of hippocampal serotonin receptor (5HT)1A and 5HT2A and brain-derived neurotrophic factor(BDNF) were measured. OJa1 and OJa2 groups had lower tail skin temperatures than OVX-control. Bone mineral density (BMD) and lean body mass (LBM) measured by DEXA increased only in OJa2, and were similar to the positive- and normal-controls ( P < 0.05). In the forced swim test immobile time, an index of depressiveness was much lower in OJa1 and OJa2 than the control group. Memory as measured by passive avoidance, water maze, and Y maze tests was impaired in the OVX-control group, compared to the normal-control ( P < 0.05), but normalized in OJa1 comparable to the positive- and normal-control groups. The neurological impairments were associated with serum serotonin levels and 5HT2A mRNA expression in the midbrain, and decreased hippocampal BDNF mRNA and protein expressions in the OVX-control group compared to normal-controls ( P < 0.05). OJa1 increased serum serotonin levels and 5HT2A expression in the midbrain, and hippocampal BDNF expression to similar levels as normal-controls ( P < 0.05). In conclusion, OJa1 and OJa2 improved hot flashes and depression and maintained BMD and LBM. OJa2 prevented the impairment of memory function in OVX rats. OJa1 and OJa2 have the potential to be effective therapies for postmenopausal vasomotor and neurological symptoms. </P><B>Impact statement</B><P> Menopausal symptoms impair the quality of life of many women, and although conventional treatments are often effective, their use is limited by adverse effects. Ojayeonjonghwan, OJa, is a traditional Oriental medicine that is used for both male and female reproductive health and has a long history of safe use. We evaluated the effectiveness of two variations of OJa (OJa1 and OJa2) for treating menopausal symptoms in ovariectomized (OVX) rats. Both OJa preparations were effective for relieving indicators of hot flashes and depression, and for preventing loss of bone mineral density and lean body mass. Only OJa 2 prevented memory dysfunction. These results show that the traditional Oriental medicine, Ojayeonjonghwan, has the potential to relieve menopausal symptoms in women and should be further evaluated in human clinical trials as an alternative to convention therapies in women for whom conventional therapies are not indicated or found to be ineffective. </P>

Verrucellin , a new diterpenoid from the gorgonian branching Verrucella umbraculum

Byoung Seob Ko,Paul J . Scheuer 한국응용생명화학회(구 한국농화학회) 1994 한국응용생명화학회 학술발표회 Vol.1994 No.1

Wnt-Signaling-Mediated Antiosteoporotic Activity of Porcine Placenta Hydrolysates in Ovariectomized Rats

Ko, Byoung-Seob,Kim, Da Sol,Kang, Suna,Lee, Na Ra,Ryuk, Jin Ah,Park, Sunmin Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-

<P>Anti-osteoporotic effects of two types of porcine placenta hydrolysates (PPH) were evaluated in ovariectomized (OVX) rats orally administered PPH without (WPPH) or with (NPPH) ovarian hormones (1 g/kg bw/day). PPH groups were compared with OVX rats with estrogen replacement (0.1 mg/kg bw conjugated estrogen; EST), or dextrose (placebo; OVX-control) All rats received high-fat/calcium-deficient diets for 12 weeks. NPPH contained less estrogen and progesterone, but more essential amino acids, whereas the opposite was true for WPPH. NPPH decreased body weight and peri-uterine fat pads, and maintained uterus weight. NPPH rats had higher femur and lumbar spine bone mass density compared to controls; but less than those of EST rats. Serum phosphorus and urinary calcium and phosphorus levels were reduced in NPPH rats compared to OVX-controls. Serum bone-specific alkaline phosphatase, osteocalcin, and bone turnover marker levels were reduced NPPH rats compared to OVX-controls. WPPH produced results similar to those of NPPH, but less significant. Both NPPH and estrogen upregulated low-density lipoprotein receptor-related protein 5 and <I><I>β</I></I>-catenin in OVX rats, while the expression of dickkopf-related protein 1 was suppressed. In conclusion, NPPH exerted anti-osteoporotic effects by activating osteogenesis and stimulating Wnt signaling, possibly mediated by the various amino acids and not ovarian hormones.</P>

The Sikchi and the recorded cases of Seungjeongwon-Ilgi

Ko Byoung Seob 한국식품연구원 2023 Journal of Ethnic Foods Vol.10 No.9

\Sikchi begins with mankind's efforts to maintain life while consuming natural food. The history of Korean food, a long experience of adapting to nature, can be found since prehistoric times. For example, Bangudae (a rock picture of hunting whales) showed how individuals gained knowledge on edible things in nature to survive. Also, Korea's specific vegetation, climate, and culture led to development of the Sikchi suitable for Koreans. The myth of Dangun of Gojoseon dynasty demonstrated the first historical record of the Sikchi. Although the Sikchi theory was established in China, Korean food therapy was systematized and developed into a unique diet that reflects our hyangyak (Korean herbal medicine) and constitution. For the king and royal family's health, Seungjeongwon-Ilgi (national-level documents of royal records) and other medical books recorded many examples of Sikchi practice, which were different from China or Japan. The wide spread of the royal Sikchi practice among citizens promoted the notion that food was a useful way to promote health and treat disease. The Sikchi should be seen as not only a kind of life-sustaining method but medicine and food-based medicinal food therapy. Further, the Sikchi refers to the treatment process of adjusting the gaps between medicine and the patient's condition with the mercenary technique called sumgoreugi (take a breather). Sikchi begins with mankind's efforts to maintain life while consuming natural food. The history of Korean food, a long experience of adapting to nature, can be found since prehistoric times. For example, Bangudae (a rock picture of hunting whales) showed how individuals gained knowledge on edible things in nature to survive. Also, Korea's specific vegetation, climate, and culture led to development of the Sikchi suitable for Koreans. The myth of Dangun of Gojoseon dynasty demonstrated the first historical record of the Sikchi. Although the Sikchi theory was established in China, Korean food therapy was systematized and developed into a unique diet that reflects our hyangyak (Korean herbal medicine) and constitution. For the king and royal family's health, Seungjeongwon-Ilgi (national-level documents of royal records) and other medical books recorded many examples of Sikchi practice, which were different from China or Japan. The wide spread of the royal Sikchi practice among citizens promoted the notion that food was a useful way to promote health and treat disease. The Sikchi should be seen as not only a kind of life-sustaining method but medicine and food-based medicinal food therapy. Further, the Sikchi refers to the treatment process of adjusting the gaps between medicine and the patient's condition with the mercenary technique called sumgoreugi (take a breather).

A 70% Ethanol Extract of Mistletoe Rich in Betulin, Betulinic Acid, and Oleanolic Acid Potentiated <i>β</i> -Cell Function and Mass and Enhanced Hepatic Insulin Sensitivity

Ko, Byoung-Seob,Kang, Suna,Moon, Bo Reum,Ryuk, Jin Ah,Park, Sunmin Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>We investigated that the long-term consumption of the water (KME-W) and 70% ethanol (KME-E) mistletoe extracts had antidiabetic activities in partial pancreatectomized (Px) rats. Px rats were provided with a high-fat diet containing 0.6% KME-E, 0.6% KME-W, and 0.6% dextrin (control) for 8 weeks. As normal-control, Sham-operated rats were provided with 0.6% dextrin. In cell-based studies, the effects of its main terpenoids (betulin, betulinic acid, and oleanolic acid) on glucose metabolism were measured. Both KME-W and KME-E decreased epididymal fat mass by increasing fat oxidation in diabetic rats. KME-E but not KME-W exhibited greater potentiation of first-phase insulin secretion than the Px-control in a hyperglycemic clamp. KME-E also made <I>β</I>-cell mass greater than the control by increasing <I>β</I>-cell proliferation and decreasing its apoptosis. In a euglycemic-hyperinsulinemic clamp, whole-body glucose infusion rate and hepatic glucose output increased with potentiating hepatic insulin signaling in the following order: Px-control, KME-W, KME-E, and normal-control. Betulin potentiated insulin-stimulated glucose uptake via increased PPAR-<I>γ</I> activity and insulin signaling in 3T3-L1 adipocytes, whereas oleanolic acid enhanced glucose-stimulated insulin secretion and cell proliferation in insulinoma cells. In conclusion, KME-E prevented the deterioration of glucose metabolism in diabetic rats more effectively than KME-W and KME-E can be a better therapeutic agent for type 2 diabetes than KME-W.</P>

<i>Prunus mume</i> and <i>Lithospermum erythrorhizon</i> Extracts Synergistically Prevent Visceral Adiposity by Improving Energy Metabolism through Potentiating Hypothalamic Leptin and Insulin Signalling in Ovariectomized Rats

Ko, Byoung-Seob,Kim, Da Sol,Kang, Suna,Ryuk, Jin Ah,Park, Sunmin Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>We investigated the antiobesity and hypoglycemic properties of <I>Prunus mume Sieb. et Zucc </I>(PMA; Japanese apricot) and <I>Lithospermum erythrorhizon Sieb. et Zucc</I> (LES; gromwell) extracts in ovariectomized (OVX) rats that impaired energy and glucose homeostasis. OVX rats consumed either 5% dextrose, 5% PMA extract, 5% LES extract, or 2.5% PMA+2.5% LES extract in the high fat diet. After 8 weeks of treatment, PMA+LES prevented weight gain and visceral fat accumulation in OVX rats by lowering daily food intake and increasing energy expenditure and fat oxidation. PMA+LES prevented the attenuation of leptin and insulin signaling by increasing the expression of leptin receptor in the hypothalamus in OVX rats. PMA+LES significantly reversed the decrease of energy expenditure in OVX rats by increasing expression of UCP-1 in the brown adipose tissues and UCP-2 and UCP-3 in the quadriceps muscles. PMA+LES also increased CPT-1 expression and decreased FAS, ACC, and SREBP-1c in the liver and quadriceps muscles to result in reducing triglyceride accumulation. PMA+LES improved insulin sensitivity in OVX rats. In conclusion, PMA+LES synergistically prevented the impairment of energy, lipid, and glucose metabolism by OVX through potentiating hypothalamic leptin and insulin signaling. PMA+LES may be a useful intervention for alleviating the symptoms of menopause in women.</P>

Contributed Papers ( Poster Abstracts ) : A Unique Fatty Acids from Marine annelid , Neanthes japonica Izuka : The C22 Diene

Byoung Seob Ko,Keun Hyung Park,Takayuki Oritani 한국응용생명화학회(구 한국농화학회) 1994 한국응용생명화학회 학술발표회 Vol.1994 No.2

Development of Biological Active Substances and Oriental Treatment of Non-insulin Dependent Diabetes Mellitus from the Recorded Herbs on the Donguibogam

Byoung Seob Ko 한국응용생명화학회 2008 Applied Biological Chemistry (Appl Biol Chem) Vol.51 No.5